Restriction map for
pFN21AB6232
(Circular) (Six-base) MAPSORT of: pFN21AB6232.seq Check: 2670 from: 1 to: 6733
pFN21AB6232 6733 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4543 278
Size: 53 83 186 936 3007 2468
Fragments arranged by size:
3007 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4076 1937
Size: 2139 4594
Acc65I G'GTAC_C
Cuts at: 4054 4054
Size: 6733
AclI AA'CG_TT
Cuts at: 1563 4860 5233 1563
Size: 3297 373 3063
Fragments arranged by size:
3297 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4796 6180 6448 1632
Size: 3164 1384 268 1917
Fragments arranged by size:
3164 1917 1384 268
AfeI AGC'GCT
Cuts at: 2940 2940
Size: 6733
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6536
AflIII A'CryG_T
Cuts at: 1203 2635 5632 6609 1203
Size: 1432 2997 977 1327
Fragments arranged by size:
2997 1432 1327 977
AgeI A'CCGG_T
Cuts at: 5564 5564
Size: 6733
AhdI GACnn_n'nnGTC
Cuts at: 5462 5462
Size: 6733
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2379 3170 3345 1184
Size: 1195 791 175 4572
Fragments arranged by size:
4572 1195 791 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 6701
AlwNI CAG_nnn'CTG
Cuts at: 2267 3928 4476 6048 2267
Size: 1661 548 1572 2952
Fragments arranged by size:
2952 1661 1572 548
ApaI G_GGCC'C
Cuts at: 2020 2067 2121 3184 2020
Size: 47 54 1063 5569
Fragments arranged by size:
5569 1063 54 47
ApaLI G'TGCA_C
Cuts at: 2650 3654 4790 5946 2650
Size: 1004 1136 1156 3437
Fragments arranged by size:
3437 1156 1136 1004
ApoI r'AATT_y
Cuts at: 1493 4042 4268 4420 6529 1493
Size: 2549 226 152 2109 1697
Fragments arranged by size:
2549 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5287 160
Size: 872 4255 1606
Fragments arranged by size:
4255 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2147 2595 2919 3017 3122 3401
Size: 73 117 87 448 324 98 105 279
Cuts at: 3401 3887 4058 1870
Size: 486 171 4545
Fragments arranged by size:
4545 486 448 324 279 171 117 105
98 87 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2624 2657 2624
Size: 33 6700
BamHI G'GATC_C
Cuts at: 4063 4430 4063
Size: 367 6366
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2272 2591 3991 4054
Size: 325 217 627 282 203 319 1400 63
Cuts at: 4054 5509 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1400 627 325 319 282 217
203 63
BanII G_rGCy'C
Cuts at: 1391 2020 2058 2067 2121 2522 2852 3184 3329
Size: 629 38 9 54 401 330 332 145
Cuts at: 3329 3543 4052 1391
Size: 214 509 4072
Fragments arranged by size:
4072 629 509 401 332 330 214 145
54 38 9
BbeI G_GCGC'C
Cuts at: 2073 3995 2073
Size: 1922 4811
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5762
BbvCI CC'TCA_GC
Cuts at: 2030 4011 2030
Size: 1981 4752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4924 4958 1903
Size: 34 2987 34 3678
Fragments arranged by size:
3678 2987 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4625 5835 4625
Size: 1210 5523
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6733
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2062 3179 3802
Size: 107 122 71 631 605 390 1117 623
Cuts at: 3802 5344 136
Size: 1542 1525
Fragments arranged by size:
1542 1525 1117 631 623 605 390 122
107 71
BglII A'GATC_T
Cuts at: 6728 6728
Size: 6733
BlpI GC'TnA_GC
Cuts at: 2781 2879 3290 4150 2781
Size: 98 411 860 5364
Fragments arranged by size:
5364 860 411 98
Bme1580I G_kGCm'C
Cuts at: 1790 2020 2067 2121 2349 2596 2654 3184 3658
Size: 230 47 54 228 247 58 530 474
Cuts at: 3658 4794 5950 1790
Size: 1136 1156 2573
Fragments arranged by size:
2573 1156 1136 530 474 247 230 228
58 54 47
BmgBI CAC'GTC
Cuts at: 1318 3727 1318
Size: 2409 4324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2496 4464 5422
Size: 416 53 83 399 24 1079 1968 958
Cuts at: 5422 442
Size: 1753
Fragments arranged by size:
1968 1753 1079 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6733
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3343 3343
Size: 6733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2506 2825 3086 3116 3402 3581 4007 5393
Size: 1267 319 261 30 286 179 426 1386
Cuts at: 5393 1239
Size: 2579
Fragments arranged by size:
2579 1386 1267 426 319 286 261 179
30
Bpu10I CC'TnA_GC
Cuts at: 2030 2516 3488 4011 2030
Size: 486 972 523 4752
Fragments arranged by size:
4752 972 523 486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 4858 5723 6021 2234
Size: 2624 865 298 2946
Fragments arranged by size:
2946 2624 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2916 5396 882
Size: 617 1417 2480 2219
Fragments arranged by size:
2480 2219 1417 617
BsaAI yAC'GTr
Cuts at: 493 1204 2542 4524 493
Size: 711 1338 1982 2702
Fragments arranged by size:
2702 1982 1338 711
BsaBI GATnn'nnATC
Cuts at: 2454 6727 2454
Size: 4273 2460
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2143 2169 3426
Size: 53 83 186 936 537 73 26 1257
Cuts at: 3426 3597 3992 4540 4922 5557 275
Size: 171 395 548 382 635 1451
Fragments arranged by size:
1451 1257 936 635 548 537 395 382
186 171 83 73 53 26
BsaWI w'CCGG_w
Cuts at: 2022 2379 3846 5166 5564 5838 5985 6396 2022
Size: 357 1467 1320 398 274 147 411 2359
Fragments arranged by size:
2359 1467 1320 411 398 357 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4010 4040 1178
Size: 30 165 30 2607 30 3871
Fragments arranged by size:
3871 2607 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 3578 1186
Size: 1594 798 4341
Fragments arranged by size:
4341 1594 798
BseYI C'CCAG_C
Cuts at: 2506 3243 3607 3902 5936 6432 2506
Size: 737 364 295 2034 496 2807
Fragments arranged by size:
2807 2034 737 496 364 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2458 4183 4944 5972 1540
Size: 275 186 457 1725 761 1028 2301
Fragments arranged by size:
2301 1725 1028 761 457 275 186
BsiHKAI G_wGCw'C
Cuts at: 2522 2550 2654 2760 3329 3645 3658 4794 4879
Size: 28 104 106 569 316 13 1136 85
Cuts at: 4879 5950 2522
Size: 1071 3305
Fragments arranged by size:
3305 1136 1071 569 316 106 104 85
28 13
BsmI GAATG_Cn'
Cuts at: 4244 4337 4244
Size: 93 6640
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2058 2067 2121 2349 2522 2550
Size: 399 230 38 9 54 228 173 28
Cuts at: 2550 2596 2654 2760 2852 3184 3329 3543 3645
Size: 46 58 106 92 332 145 214 102
Cuts at: 3645 3658 4052 4794 4879 5950 1391
Size: 13 394 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 332 230 228
214 173 145 106 102 92 85 58
54 46 38 28 13 9
BspEI T'CCGG_A
Cuts at: 2022 2379 6396 2022
Size: 357 4017 2359
Fragments arranged by size:
4017 2359 357
BspHI T'CATG_A
Cuts at: 4622 6352 4622
Size: 1730 5003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4088 844
Size: 3244 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4620 1394
Size: 429 2797 3507
Fragments arranged by size:
3507 2797 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3831 5228 5402 65
Size: 1183 2583 1397 174 1396
Fragments arranged by size:
2583 1397 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2997 3630 5377 5564 1700
Size: 110 1187 633 1747 187 2869
Fragments arranged by size:
2869 1747 1187 633 187 110
BsrGI T'GTAC_A
Cuts at: 96 2943 96
Size: 2847 3886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6733
BssSI C'ACGA_G
Cuts at: 3575 3998 4793 5805 3575
Size: 423 795 1012 4503
Fragments arranged by size:
4503 1012 795 423
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6733
BstBI TT'CG_AA
Cuts at: 5537 5537
Size: 6733
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6733
BstYI r'GATC_y
Cuts at: 1972 4063 4430 4815 4832 6284 6370 6382 6728
Size: 2091 367 385 17 1452 86 12 346
Cuts at: 6728 1972
Size: 1977
Fragments arranged by size:
2091 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2621 2802 3612 3820 3870 513
Size: 552 1556 181 810 208 50 3376
Fragments arranged by size:
3376 1556 810 552 208 181 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2898 487
Size: 683 56 1672 4322
Fragments arranged by size:
4322 1672 683 56
BtsI GCAGTG_nn'
Cuts at: 3418 3597 4249 5043 5063 3418
Size: 179 652 794 20 5088
Fragments arranged by size:
5088 794 652 179 20
ClaI AT'CG_AT
Cuts at: 1554 2454 1554
Size: 900 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6698
DraI TTT'AAA
Cuts at: 4037 4037
Size: 6733
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3736 5740 809
Size: 507 2420 2004 1802
Fragments arranged by size:
2420 2004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2047 2799 3963
Size: 54 1425 50 275 18 217 752 1164
Cuts at: 3963 4180 5069 6698 8
Size: 217 889 1629 43
Fragments arranged by size:
1629 1425 1164 889 752 275 217 217
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4180 1537
Size: 275 2368 4090
Fragments arranged by size:
4090 2368 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3215 3845 4663 1353
Size: 1862 630 818 3423
Fragments arranged by size:
3423 1862 818 630
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3194 3649 5320 5694 5840 387
Size: 1064 138 1605 455 1671 374 146 1280
Fragments arranged by size:
1671 1605 1280 1064 455 374 146 138
EcoICRI GAG'CTC
Cuts at: 2520 3327 2520
Size: 807 5926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2506 2825 3086 3116 3402 3581 4007
Size: 393 874 319 261 30 286 179 426
Cuts at: 4007 4796 5393 6180 6448 1239
Size: 789 597 787 268 1524
Fragments arranged by size:
1524 874 789 787 597 426 393 319
286 268 261 179 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3212 3790 3212
Size: 578 6155
EcoO109I rG'GnC_Cy
Cuts at: 2017 2117 2991 3136 3604 3925 2017
Size: 100 874 145 468 321 4825
Fragments arranged by size:
4825 874 468 321 145 100
EcoRI G'AATT_C
Cuts at: 4042 4420 4042
Size: 378 6355
EcoRV GAT'ATC
Cuts at: 2452 2452
Size: 6733
FspI TGC'GCA
Cuts at: 5239 5239
Size: 6733
HaeII r_GCGC'y
Cuts at: 1678 2073 2942 3095 3368 3995 5880 1678
Size: 395 869 153 273 627 1885 2531
Fragments arranged by size:
2531 1885 869 627 395 273 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3793 3825 5374 5406 5448
Size: 32 135 32 2221 32 1549 32 42
Cuts at: 5448 5480 1373
Size: 32 2626
Fragments arranged by size:
2626 2221 1549 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2179 2863 4077 4323 669
Size: 988 281 241 684 1214 246 3079
Fragments arranged by size:
3079 1214 988 684 281 246 241
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6733
HpaI GTT'AAC
Cuts at: 4323 4323
Size: 6733
KasI G'GCGC_C
Cuts at: 2069 3991 2069
Size: 1922 4811
KpnI G_GTAC'C
Cuts at: 4058 4058
Size: 6733
MfeI C'AATT_G
Cuts at: 4332 4332
Size: 6733
MluI A'CGCG_T
Cuts at: 6609 6609
Size: 6733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5847 6031 1305
Size: 4542 184 2007
Fragments arranged by size:
4542 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3965 10
Size: 54 1425 343 2133 2778
Fragments arranged by size:
2778 2133 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 3675 4691 5050 5209 518
Size: 648 1201 1308 1016 359 159 2042
Fragments arranged by size:
2042 1308 1201 1016 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3496 4149 4826 5974 6219 1606
Size: 1890 653 677 1148 245 2120
Fragments arranged by size:
2120 1890 1148 677 653 245
NaeI GCC'GGC
Cuts at: 2999 2999
Size: 6733
NarI GG'CG_CC
Cuts at: 2070 3992 2070
Size: 1922 4811
NcoI C'CATG_G
Cuts at: 513 1065 3820 3870 513
Size: 552 2755 50 3376
Fragments arranged by size:
3376 2755 552 50
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6733
NgoMIV G'CCGG_C
Cuts at: 2997 2997
Size: 6733
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6733
NotI GC'GGCC_GC
Cuts at: 4180 4180
Size: 6733
NspI r_CATG'y
Cuts at: 1132 1229 3151 4091 5636 1132
Size: 97 1922 940 1545 2229
Fragments arranged by size:
2229 1922 1545 940 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6733
PciI A'CATG_T
Cuts at: 5632 5632
Size: 6733
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3555 1108
Size: 2447 4286
PfoI T'CCnGG_A
Cuts at: 2522 3063 3213 2522
Size: 541 150 6042
Fragments arranged by size:
6042 541 150
PmeI GTTT'AAAC
Cuts at: 4037 4037
Size: 6733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4802 4834 6315 6347 4802
Size: 32 1481 32 5188
Fragments arranged by size:
5188 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3136 3604 3136
Size: 468 6265
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6733
PsiI TTA'TAA
Cuts at: 4303 4303
Size: 6733
PspOMI G'GGCC_C
Cuts at: 2016 2063 2117 3180 2016
Size: 47 54 1063 5569
Fragments arranged by size:
5569 1063 54 47
PspXI vC'TCGA_Gb
Cuts at: 3401 3401
Size: 6733
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 6701
PstI C_TGCA'G
Cuts at: 830 2877 4085 830
Size: 2047 1208 3478
Fragments arranged by size:
3478 2047 1208
PvuI CG_AT'CG
Cuts at: 2001 2458 2001
Size: 457 6276
PvuII CAG'CTG
Cuts at: 3496 3496
Size: 6733
SacI G_AGCT'C
Cuts at: 2522 3329 2522
Size: 807 5926
SalI G'TCGA_C
Cuts at: 1936 4075 1936
Size: 2139 4594
SanDI GG'GwC_CC
Cuts at: 3604 3604
Size: 6733
SbfI CC_TGCA'GG
Cuts at: 4085 4085
Size: 6733
ScaI AGT'ACT
Cuts at: 3101 4981 6693 3101
Size: 1880 1712 3141
Fragments arranged by size:
3141 1880 1712
SexAI A'CCwGG_T
Cuts at: 2698 2698
Size: 6733
SfcI C'TryA_G
Cuts at: 826 1045 2689 2873 4081 5216 5897 6088 826
Size: 219 1644 184 1208 1135 681 191 1471
Fragments arranged by size:
1644 1471 1208 1135 681 219 191 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 3179 3179
Size: 6733
SfoI GGC'GCC
Cuts at: 2071 3993 2071
Size: 1922 4811
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6733
SmaI CCC'GGG
Cuts at: 1872 2149 2597 4060 1872
Size: 277 448 1463 4545
Fragments arranged by size:
4545 1463 448 277
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 2919 3122 3401 4837 5738
Size: 197 926 270 706 203 279 1436 901
Cuts at: 5738 6000 820
Size: 262 1553
Fragments arranged by size:
1553 1436 926 901 706 279 270 262
203 197
SnaBI TAC'GTA
Cuts at: 493 4524 493
Size: 4031 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6733
SphI G_CATG'C
Cuts at: 1132 3151 4091 1132
Size: 2019 940 3774
Fragments arranged by size:
3774 2019 940
SrfI GCCC'GGGC
Cuts at: 2597 2597
Size: 6733
SspI AAT'ATT
Cuts at: 5 52 4657 5
Size: 47 4605 2081
Fragments arranged by size:
4605 2081 47
StuI AGG'CCT
Cuts at: 1517 2736 3344 1517
Size: 1219 608 4906
Fragments arranged by size:
4906 1219 608
StyI C'CwwG_G
Cuts at: 513 1065 3511 3820 3870 4172 513
Size: 552 2446 309 50 302 3074
Fragments arranged by size:
3074 2446 552 309 302 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3673 4760 4777 4930 5115 5560 325
Size: 1255 2093 1087 17 153 185 445 1498
Fragments arranged by size:
2093 1498 1255 1087 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2943 3099 4979
Size: 274 80 33 51 1443 966 156 1880
Cuts at: 4979 6691 96
Size: 1712 138
Fragments arranged by size:
1880 1712 1443 966 274 156 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4010 4042 6693 6725 4010
Size: 32 2651 32 4018
Fragments arranged by size:
4018 2651 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6733
XbaI T'CTAG_A
Cuts at: 4069 4069
Size: 6733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2716 3556 2716
Size: 840 5893
XhoI C'TCGA_G
Cuts at: 1943 2919 3122 3401 1943
Size: 976 203 279 5275
Fragments arranged by size:
5275 976 279 203
XmaI C'CCGG_G
Cuts at: 1870 2147 2595 4058 1870
Size: 277 448 1463 4545
Fragments arranged by size:
4545 1463 448 277
XmnI GAAnn'nnTTC
Cuts at: 4862 4862
Size: 6733
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4541 276
Size: 53 83 186 936 3007 2468
Fragments arranged by size:
3007 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BfrBI BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII FalI FseI FspAI NruI NsiI PacI
PmlI RsrII SacII SapI SwaI