Restriction map for
pFN21AB6231
(Circular) (Six-base) MAPSORT of: pFN21AB6231.seq Check: 4642 from: 1 to: 6934
pFN21AB6231 6934 bp
With 182 enzymes: SgfI *
March 28, 2012 12:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4744 278
Size: 53 83 186 936 3208 2468
Fragments arranged by size:
3208 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4277 1937
Size: 2340 4594
Acc65I G'GTAC_C
Cuts at: 4255 4255
Size: 6934
AclI AA'CG_TT
Cuts at: 1563 5061 5434 1563
Size: 3498 373 3063
Fragments arranged by size:
3498 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4997 6381 6649 1632
Size: 3365 1384 268 1917
Fragments arranged by size:
3365 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6737
AflIII A'CryG_T
Cuts at: 1203 3819 5833 6810 1203
Size: 2616 2014 977 1327
Fragments arranged by size:
2616 2014 1327 977
AgeI A'CCGG_T
Cuts at: 3656 5765 3656
Size: 2109 4825
AhdI GACnn_n'nnGTC
Cuts at: 3295 5663 3295
Size: 2368 4566
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3983 1184
Size: 2799 4135
AlwNI CAG_nnn'CTG
Cuts at: 3712 3778 4677 6249 3712
Size: 66 899 1572 4397
Fragments arranged by size:
4397 1572 899 66
ApaI G_GGCC'C
Cuts at: 2253 3494 4011 2253
Size: 1241 517 5176
Fragments arranged by size:
5176 1241 517
ApaLI G'TGCA_C
Cuts at: 2318 3872 4991 6147 2318
Size: 1554 1119 1156 3105
Fragments arranged by size:
3105 1554 1156 1119
ApoI r'AATT_y
Cuts at: 1493 2495 2834 4243 4469 4621 6730 1493
Size: 1002 339 1409 226 152 2109 1697
Fragments arranged by size:
2109 1697 1409 1002 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5488 160
Size: 872 4456 1606
Fragments arranged by size:
4456 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6934
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 3270 3424 3517 3664 4066 4259
Size: 73 1221 106 154 93 147 402 193
Cuts at: 4259 1870
Size: 4545
Fragments arranged by size:
4545 1221 402 193 154 147 106 93
73
BamHI G'GATC_C
Cuts at: 4264 4631 4264
Size: 367 6567
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 2177 2363 3549 3590
Size: 325 217 627 290 100 186 1186 41
Cuts at: 3590 3943 4255 5710 618
Size: 353 312 1455 1842
Fragments arranged by size:
1842 1455 1186 627 353 325 312 290
217 186 100 41
BanII G_rGCy'C
Cuts at: 1391 2253 2289 3213 3374 3494 4011 4073 4253
Size: 862 36 924 161 120 517 62 180
Cuts at: 4253 1391
Size: 4072
Fragments arranged by size:
4072 924 862 517 180 161 120 62
36
BbeI G_GCGC'C
Cuts at: 2081 2181 3594 2081
Size: 100 1413 5421
Fragments arranged by size:
5421 1413 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 928
Size: 971 383 5580
Fragments arranged by size:
5580 971 383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4047 4081 5125 5159 1903
Size: 34 2110 34 1044 34 3678
Fragments arranged by size:
3678 2110 1044 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4826 6036 4826
Size: 1210 5724
BclI T'GATC_A
Cuts at: 1548 2680 1548
Size: 1132 5802
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3528 3701 3836
Size: 107 122 71 631 605 1856 173 135
Cuts at: 3836 4034 4046 4055 5545 136
Size: 198 12 9 1490 1525
Fragments arranged by size:
1856 1525 1490 631 605 198 173 135
122 107 71 12 9
BglII A'GATC_T
Cuts at: 3186 6929 3186
Size: 3743 3191
BlpI GC'TnA_GC
Cuts at: 3261 4351 3261
Size: 1090 5844
Bme1580I G_kGCm'C
Cuts at: 1790 2253 2322 2366 3389 3494 3552 3876 4011
Size: 463 69 44 1023 105 58 324 135
Cuts at: 4011 4995 6151 1790
Size: 984 1156 2573
Fragments arranged by size:
2573 1156 1023 984 463 324 135 105
69 58 44
BmgBI CAC'GTC
Cuts at: 1318 3201 3760 1318
Size: 1883 559 4492
Fragments arranged by size:
4492 1883 559
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2592 2869 4665
Size: 416 53 83 399 24 1175 277 1796
Cuts at: 4665 5623 442
Size: 958 1753
Fragments arranged by size:
1796 1753 1175 958 416 399 277 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6934
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 3007 3051 3583 3734 3847 4090 5594
Size: 1106 662 44 532 151 113 243 1504
Cuts at: 5594 1239
Size: 2579
Fragments arranged by size:
2579 1504 1106 662 532 243 151 113
44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5059 5924 6222 5059
Size: 865 298 5771
Fragments arranged by size:
5771 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 5597 882
Size: 617 717 3381 2219
Fragments arranged by size:
3381 2219 717 617
BsaAI yAC'GTr
Cuts at: 493 1204 2691 3822 4725 493
Size: 711 1487 1131 903 2702
Fragments arranged by size:
2702 1487 1131 903 711
BsaBI GATnn'nnATC
Cuts at: 6928 6928
Size: 6934
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 2178 3132 3591
Size: 53 83 186 936 545 100 954 459
Cuts at: 3591 3722 4741 5123 5758 275
Size: 131 1019 382 635 1451
Fragments arranged by size:
1451 1019 954 936 635 545 459 382
186 131 100 83 53
BsaWI w'CCGG_w
Cuts at: 2026 3656 5367 5765 6039 6186 6597 2026
Size: 1630 1711 398 274 147 411 2363
Fragments arranged by size:
2363 1711 1630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2128 2158 4073 4103 1178
Size: 30 165 30 725 30 1915 30 4009
Fragments arranged by size:
4009 1915 725 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 3167 3221 3374 3838 4081 4093 1186
Size: 1044 937 54 153 464 243 12 4027
Fragments arranged by size:
4027 1044 937 464 243 153 54 12
BseYI C'CCAG_C
Cuts at: 2104 3113 3569 6137 6633 2104
Size: 1009 456 2568 496 2405
Fragments arranged by size:
2568 2405 1009 496 456
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3476 3548 1163
Size: 573 1740 72 4549
Fragments arranged by size:
4549 1740 573 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2220 3471 3612 3843 4002 4384
Size: 275 186 219 1251 141 231 159 382
Cuts at: 4384 5145 6173 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1251 1028 761 382 275 231 219
186 159 141
BsiHKAI G_wGCw'C
Cuts at: 2322 3876 4995 5080 6151 2322
Size: 1554 1119 85 1071 3105
Fragments arranged by size:
3105 1554 1119 1071 85
BsmI GAATG_Cn'
Cuts at: 4445 4538 4445
Size: 93 6841
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2289 2322 2366 3213 3374 3389
Size: 399 463 36 33 44 847 161 15
Cuts at: 3389 3494 3552 3876 4011 4073 4253 4995 5080
Size: 105 58 324 135 62 180 742 85
Cuts at: 5080 6151 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 847 742 463 399 324 180
161 135 105 85 62 58 44 36
33 15
BspEI T'CCGG_A
Cuts at: 6597 6597
Size: 6934
BspHI T'CATG_A
Cuts at: 4823 6553 4823
Size: 1730 5204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4289 844
Size: 3445 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3265 3448 3556 4089 4821 1394
Size: 429 1442 183 108 533 732 3507
Fragments arranged by size:
3507 1442 732 533 429 183 108
BsrDI GCAATG_nn'
Cuts at: 65 1248 5429 5603 65
Size: 1183 4181 174 1396
Fragments arranged by size:
4181 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2225 3394 3656 3946 5578 5765 1700
Size: 110 415 1169 262 290 1632 187 2869
Fragments arranged by size:
2869 1632 1169 415 290 262 187 110
BsrGI T'GTAC_A
Cuts at: 96 2556 96
Size: 2460 4474
BssHII G'CGCG_C
Cuts at: 1925 3413 3415 3417 1925
Size: 1488 2 2 5442
Fragments arranged by size:
5442 1488 2 2
BssSI C'ACGA_G
Cuts at: 4994 6006 4994
Size: 1012 5922
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6934
BstBI TT'CG_AA
Cuts at: 5738 5738
Size: 6934
BstEII G'GTnAC_C
Cuts at: 1184 2108 1184
Size: 924 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 3685 3685
Size: 6934
BstYI r'GATC_y
Cuts at: 1972 2023 3186 4264 4631 5016 5033 6485 6571
Size: 51 1163 1078 367 385 17 1452 86
Cuts at: 6571 6583 6929 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1163 1078 385 367 346 86
51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2280 3234 3582 3636 3678 3684 3726
Size: 552 1215 954 348 54 42 6 42
Cuts at: 3726 3911 3990 4002 513
Size: 185 79 12 3445
Fragments arranged by size:
3445 1215 954 552 348 185 79 54
42 42 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2824 487
Size: 683 56 1598 4597
Fragments arranged by size:
4597 1598 683 56
BtsI GCAGTG_nn'
Cuts at: 4065 4450 5244 5264 4065
Size: 385 794 20 5735
Fragments arranged by size:
5735 794 385 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6934
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6899
DraI TTT'AAA
Cuts at: 2413 4238 2413
Size: 1825 5109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4022 5941 809
Size: 507 2706 1919 1802
Fragments arranged by size:
2706 1919 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2217 2929
Size: 54 1425 50 275 18 354 33 712
Cuts at: 2929 3180 3396 3468 3609 3633 3681 3729 3780
Size: 251 216 72 141 24 48 48 51
Cuts at: 3780 3828 3840 3999 4035 4152 4381 5270 6899
Size: 48 12 159 36 117 229 889 1629
Cuts at: 6899 8
Size: 43
Fragments arranged by size:
1629 1425 889 712 354 275 251 229
216 159 141 117 72 54 51 50
48 48 48 43 36 33 24 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 2217 3468 3609 3840 3999 4381 1537
Size: 275 405 1251 141 231 159 382 4090
Fragments arranged by size:
4090 1251 405 382 275 231 159 141
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 2929 3402 3563 4864 1353
Size: 936 640 473 161 1301 3423
Fragments arranged by size:
3423 1301 936 640 473 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5521 5895 6041 387
Size: 1064 138 3932 374 146 1280
Fragments arranged by size:
3932 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 3007 3051 3583 3734 3847 4090
Size: 393 713 662 44 532 151 113 243
Cuts at: 4090 4997 5594 6381 6649 1239
Size: 907 597 787 268 1524
Fragments arranged by size:
1524 907 787 713 662 597 532 393
268 243 151 113 44
EcoNI CCTnn'n_nnAGG
Cuts at: 3485 3485
Size: 6934
EcoO109I rG'GnC_Cy
Cuts at: 2249 2250 3006 3490 3491 3623 3648 3775 4007
Size: 1 756 484 1 132 25 127 232
Cuts at: 4007 4008 4215 2249
Size: 1 207 4968
Fragments arranged by size:
4968 756 484 232 207 132 127 25
1 1 1
EcoRI G'AATT_C
Cuts at: 2834 4243 4621 2834
Size: 1409 378 5147
Fragments arranged by size:
5147 1409 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3454 3454
Size: 6934
FspI TGC'GCA
Cuts at: 5440 5440
Size: 6934
HaeII r_GCGC'y
Cuts at: 1678 2081 2165 2181 3395 3594 6081 1678
Size: 403 84 16 1214 199 2487 2531
Fragments arranged by size:
2531 2487 1214 403 199 84 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2128 2160 2185
Size: 32 135 32 441 32 83 32 25
Cuts at: 2185 2217 3005 3037 3281 3313 4055 4087 5575
Size: 32 788 32 244 32 742 32 1488
Cuts at: 5575 5607 5649 5681 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 788 742 441 244 135 83
42 32 32 32 32 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 4278 4524 669
Size: 988 281 2340 246 3079
Fragments arranged by size:
3079 2340 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6934
HpaI GTT'AAC
Cuts at: 4524 4524
Size: 6934
KasI G'GCGC_C
Cuts at: 2077 2177 3590 2077
Size: 100 1413 5421
Fragments arranged by size:
5421 1413 100
KpnI G_GTAC'C
Cuts at: 4259 4259
Size: 6934
MfeI C'AATT_G
Cuts at: 2204 4533 2204
Size: 2329 4605
MluI A'CGCG_T
Cuts at: 6810 6810
Size: 6934
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3183 6048 6232 1305
Size: 1878 2865 184 2007
Fragments arranged by size:
2865 2007 1878 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 3635 3782 10
Size: 54 1425 343 354 1449 147 3162
Fragments arranged by size:
3162 1449 1425 354 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 2843 2972 3683 3824 3941 4892
Size: 648 1203 474 129 711 141 117 951
Cuts at: 4892 5251 5410 518
Size: 359 159 2042
Fragments arranged by size:
2042 1203 951 711 648 474 359 159
141 129 117
MspA1I CmG'CkG
Cuts at: 1606 3236 3279 3614 3698 3728 3836 3913 4004
Size: 1630 43 335 84 30 108 77 91
Cuts at: 4004 4180 4350 5027 6175 6420 1606
Size: 176 170 677 1148 245 2120
Fragments arranged by size:
2120 1630 1148 677 335 245 176 170
108 91 84 77 43 30
NaeI GCC'GGC
Cuts at: 3396 3396
Size: 6934
NarI GG'CG_CC
Cuts at: 2078 2178 3591 2078
Size: 100 1413 5421
Fragments arranged by size:
5421 1413 100
NcoI C'CATG_G
Cuts at: 513 1065 2280 3582 3684 3990 513
Size: 552 1215 1302 102 306 3457
Fragments arranged by size:
3457 1302 1215 552 306 102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6934
NgoMIV G'CCGG_C
Cuts at: 3394 3394
Size: 6934
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6934
NotI GC'GGCC_GC
Cuts at: 3468 3609 3840 3999 4381 3468
Size: 141 231 159 382 6021
Fragments arranged by size:
6021 382 231 159 141
NruI TCG'CGA
Cuts at: 3381 3381
Size: 6934
NspI r_CATG'y
Cuts at: 1132 1229 4292 5837 1132
Size: 97 3063 1545 2229
Fragments arranged by size:
3063 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2245 3487 1348
Size: 897 1242 4795
Fragments arranged by size:
4795 1242 897
PciI A'CATG_T
Cuts at: 5833 5833
Size: 6934
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4183 1108
Size: 3075 3859
PfoI T'CCnGG_A
Cuts at: 2913 3951 2913
Size: 1038 5896
PmeI GTTT'AAAC
Cuts at: 4238 4238
Size: 6934
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5003 5035 6516 6548 5003
Size: 32 1481 32 5389
Fragments arranged by size:
5389 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3006 3623 4215 3006
Size: 617 592 5725
Fragments arranged by size:
5725 617 592
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6934
PsiI TTA'TAA
Cuts at: 2493 4504 2493
Size: 2011 4923
PspOMI G'GGCC_C
Cuts at: 2249 3490 4007 2249
Size: 1241 517 5176
Fragments arranged by size:
5176 1241 517
PstI C_TGCA'G
Cuts at: 830 2964 4170 4286 830
Size: 2134 1206 116 3478
Fragments arranged by size:
3478 2134 1206 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6934
SacII CC_GC'GG
Cuts at: 3237 3729 3914 4005 3237
Size: 492 185 91 6166
Fragments arranged by size:
6166 492 185 91
SalI G'TCGA_C
Cuts at: 1936 4276 1936
Size: 2340 4594
SanDI GG'GwC_CC
Cuts at: 3623 3623
Size: 6934
SapI GCTCTTCn'nnn_
Cuts at: 3563 3563
Size: 6934
SbfI CC_TGCA'GG
Cuts at: 4286 4286
Size: 6934
ScaI AGT'ACT
Cuts at: 5182 6894 5182
Size: 1712 5222
SfcI C'TryA_G
Cuts at: 826 1045 2960 3081 3922 4166 4282 5417 6098
Size: 219 1915 121 841 244 116 1135 681
Cuts at: 6098 6289 826
Size: 191 1471
Fragments arranged by size:
1915 1471 1135 841 681 244 219 191
121 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 3528 3528
Size: 6934
SfoI GGC'GCC
Cuts at: 2079 2179 3592 2079
Size: 100 1413 5421
Fragments arranged by size:
5421 1413 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6934
SgrAI Cr'CCGG_yG
Cuts at: 1700 3656 1700
Size: 1956 4978
SmaI CCC'GGG
Cuts at: 1872 3272 3426 3519 4261 1872
Size: 1400 154 93 742 4545
Fragments arranged by size:
4545 1400 742 154 93
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5038 5939 6201 820
Size: 197 926 3095 901 262 1553
Fragments arranged by size:
3095 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4725 493
Size: 4232 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6934
SphI G_CATG'C
Cuts at: 1132 4292 1132
Size: 3160 3774
SspI AAT'ATT
Cuts at: 5 52 4858 5
Size: 47 4806 2081
Fragments arranged by size:
4806 2081 47
StuI AGG'CCT
Cuts at: 1517 2269 3170 3437 1517
Size: 752 901 267 5014
Fragments arranged by size:
5014 901 752 267
StyI C'CwwG_G
Cuts at: 513 1065 2280 2313 3399 3582 3684 3990 4373
Size: 552 1215 33 1086 183 102 306 383
Cuts at: 4373 513
Size: 3074
Fragments arranged by size:
3074 1215 1086 552 383 306 183 102
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4961 4978 5131 5316 5761 325
Size: 1255 3381 17 153 185 445 1498
Fragments arranged by size:
3381 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 2801 5180
Size: 274 80 33 51 1443 579 245 2379
Cuts at: 5180 6892 96
Size: 1712 138
Fragments arranged by size:
2379 1712 1443 579 274 245 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6894 6926 6894
Size: 32 6902
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6934
XbaI T'CTAG_A
Cuts at: 4270 4270
Size: 6934
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2320 2194
Size: 126 6808
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6934
XmaI C'CCGG_G
Cuts at: 1870 3270 3424 3517 4259 1870
Size: 1400 154 93 742 4545
Fragments arranged by size:
4545 1400 742 154 93
XmnI GAAnn'nnTTC
Cuts at: 5063 5063
Size: 6934
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4742 276
Size: 53 83 186 936 3208 2468
Fragments arranged by size:
3208 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SexAI SrfI SwaI