Restriction map for
pFN21AB6227
(Circular) (Six-base) MAPSORT of: pFN21AB6227.seq Check: 109 from: 1 to: 6721
pFN21AB6227 6721 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2414 2414
Size: 6721
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4064 1937
Size: 2127 4594
Acc65I G'GTAC_C
Cuts at: 4042 4042
Size: 6721
AclI AA'CG_TT
Cuts at: 1563 2418 3666 4848 5221 1563
Size: 855 1248 1182 373 3063
Fragments arranged by size:
3063 1248 1182 855 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 2988 3134 3416 4784 6168 6436 1632
Size: 1056 300 146 282 1368 1384 268 1917
Fragments arranged by size:
1917 1384 1368 1056 300 282 268 146
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6524
AflIII A'CryG_T
Cuts at: 1203 5620 6597 1203
Size: 4417 977 1327
Fragments arranged by size:
4417 1327 977
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 3908 5450 3908
Size: 1542 5179
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6721
AlwNI CAG_nnn'CTG
Cuts at: 2160 2454 4464 6036 2160
Size: 294 2010 1572 2845
Fragments arranged by size:
2845 2010 1572 294
ApaLI G'TGCA_C
Cuts at: 2096 3151 3995 4778 5934 2096
Size: 1055 844 783 1156 2883
Fragments arranged by size:
2883 1156 1055 844 783
ApoI r'AATT_y
Cuts at: 1493 2117 2243 2915 3305 4030 4256 4408 6517
Size: 624 126 672 390 725 226 152 2109
Cuts at: 6517 1493
Size: 1697
Fragments arranged by size:
2109 1697 725 672 624 390 226 152
126
AseI AT'TA_AT
Cuts at: 160 1032 5275 160
Size: 872 4243 1606
Fragments arranged by size:
4243 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 4046 1870
Size: 73 77 2026 4545
Fragments arranged by size:
4545 2026 77 73
BamHI G'GATC_C
Cuts at: 2563 2774 4051 4418 2563
Size: 211 1277 367 4866
Fragments arranged by size:
4866 1277 367 211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 4042 5497 618
Size: 325 217 627 448 1807 1455 1842
Fragments arranged by size:
1842 1807 1455 627 448 325 217
BanII G_rGCy'C
Cuts at: 1391 4040 1391
Size: 2649 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2976 3639 928
Size: 971 1077 663 4010
Fragments arranged by size:
4010 1077 971 663
BbvCI CC'TCA_GC
Cuts at: 2081 2502 2081
Size: 421 6300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4912 4946 1903
Size: 34 2975 34 3678
Fragments arranged by size:
3678 2975 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4613 5823 4613
Size: 1210 5511
BclI T'GATC_A
Cuts at: 1548 3925 1548
Size: 2377 4344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2943 5332 136
Size: 107 122 71 631 605 1271 2389 1525
Fragments arranged by size:
2389 1525 1271 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6716 6716
Size: 6721
BlpI GC'TnA_GC
Cuts at: 4138 4138
Size: 6721
Bme1580I G_kGCm'C
Cuts at: 1790 2100 3086 3155 3999 4782 5938 1790
Size: 310 986 69 844 783 1156 2573
Fragments arranged by size:
2573 1156 986 844 783 310 69
BmgBI CAC'GTC
Cuts at: 1318 2050 3145 3775 1318
Size: 732 1095 630 4264
Fragments arranged by size:
4264 1095 732 630
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2752 3817 3982
Size: 416 53 83 399 24 1335 1065 165
Cuts at: 3982 4452 5410 442
Size: 470 958 1753
Fragments arranged by size:
1753 1335 1065 958 470 416 399 165
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6721
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3026
Size: 6721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3492 3839 5381 1239
Size: 2253 347 1542 2579
Fragments arranged by size:
2579 2253 1542 347
Bpu10I CC'TnA_GC
Cuts at: 2081 2502 2081
Size: 421 6300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4846 5711 6009 4846
Size: 865 298 5558
Fragments arranged by size:
5558 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5384 882
Size: 617 3885 2219
Fragments arranged by size:
3885 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4512 493
Size: 711 3308 2702
Fragments arranged by size:
3308 2702 711
BsaBI GATnn'nnATC
Cuts at: 3347 6715 3347
Size: 3368 3353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2039 3579 4528 4910
Size: 53 83 186 936 506 1540 949 382
Cuts at: 4910 5545 275
Size: 635 1451
Fragments arranged by size:
1540 1451 949 936 635 506 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2875 3795 5154 5552 5826 5973 6384 2875
Size: 920 1359 398 274 147 411 3212
Fragments arranged by size:
3212 1359 920 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6496
Fragments arranged by size:
6496 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6721
BseYI C'CCAG_C
Cuts at: 2447 3733 3745 5924 6420 2447
Size: 1286 12 2179 496 2748
Fragments arranged by size:
2748 2179 1286 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2146 3197 4035 1163
Size: 573 410 1051 838 3849
Fragments arranged by size:
3849 1051 838 573 410
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4171 4932 5960 1540
Size: 275 186 2170 761 1028 2301
Fragments arranged by size:
2301 2170 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 3155 3663 3999 4782 4867 5938 2100
Size: 1055 508 336 783 85 1071 2883
Fragments arranged by size:
2883 1071 1055 783 508 336 85
BsmI GAATG_Cn'
Cuts at: 4232 4325 4232
Size: 93 6628
BsmBI CGTCTCn'nnnn_
Cuts at: 2046 2046
Size: 6721
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 3086 3155 3663 3999 4040 4782
Size: 399 310 986 69 508 336 41 742
Cuts at: 4782 4867 5938 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 986 742 508 399 336 310
85 69 41
BspEI T'CCGG_A
Cuts at: 2875 3795 6384 2875
Size: 920 2589 3212
Fragments arranged by size:
3212 2589 920
BspHI T'CATG_A
Cuts at: 4610 6340 4610
Size: 1730 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2414 4076 844
Size: 1570 1662 3489
Fragments arranged by size:
3489 1662 1570
BsrBI CCG'CTC
Cuts at: 1394 1823 2652 4608 1394
Size: 429 829 1956 3507
Fragments arranged by size:
3507 1956 829 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2435 5216 5390 65
Size: 1183 1187 2781 174 1396
Fragments arranged by size:
2781 1396 1187 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2812 5365 5552 1700
Size: 110 1002 2553 187 2869
Fragments arranged by size:
2869 2553 1002 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6721
BssHII G'CGCG_C
Cuts at: 1925 2126 1925
Size: 201 6520
BssSI C'ACGA_G
Cuts at: 2099 3897 4781 5793 2099
Size: 1798 884 1012 3027
Fragments arranged by size:
3027 1798 1012 884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2160 794
Size: 1366 5355
BstBI TT'CG_AA
Cuts at: 5525 5525
Size: 6721
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6721
BstXI CCAn_nnnn'nTGG
Cuts at: 3745 3745
Size: 6721
BstYI r'GATC_y
Cuts at: 1972 2563 2581 2774 3391 3831 4051 4418 4803
Size: 591 18 193 617 440 220 367 385
Cuts at: 4803 4820 6272 6358 6370 6716 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 617 591 440 385 367 346
220 193 86 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 3759 3759
Size: 6721
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 3156 513
Size: 552 939 30 1122 4078
Fragments arranged by size:
4078 1122 939 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2107 487
Size: 683 56 881 5101
Fragments arranged by size:
5101 881 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 2363 2675 4237 5031 5051 2158
Size: 205 312 1562 794 20 3828
Fragments arranged by size:
3828 1562 794 312 205 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3841 3876 529
Size: 35 3277 35 3374
Fragments arranged by size:
3374 3277 35 35
DraI TTT'AAA
Cuts at: 4025 4025
Size: 6721
DraIII CAC_nnn'GTG
Cuts at: 2104 2104
Size: 6721
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5728 809
Size: 507 4412 1802
Fragments arranged by size:
4412 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2814 4168
Size: 54 1425 50 275 18 222 762 1354
Cuts at: 4168 5057 6686 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1354 889 762 275 222 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4168 1537
Size: 275 2356 4090
Fragments arranged by size:
4090 2356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2471 4651 1353
Size: 1118 2180 3423
Fragments arranged by size:
3423 2180 1118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2500 5308 5682 5828 387
Size: 1064 138 911 2808 374 146 1280
Fragments arranged by size:
2808 1280 1064 911 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 2988 3134 3416 3492 3839 4784
Size: 393 1056 300 146 282 76 347 945
Cuts at: 4784 5381 6168 6436 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1056 945 787 597 393 347 300
282 268 146 76
EcoNI CCTnn'n_nnAGG
Cuts at: 3116 3116
Size: 6721
EcoO109I rG'GnC_Cy
Cuts at: 2279 3274 3492 2279
Size: 995 218 5508
Fragments arranged by size:
5508 995 218
EcoRI G'AATT_C
Cuts at: 4030 4408 4030
Size: 378 6343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2948 3190 2948
Size: 242 6479
FspI TGC'GCA
Cuts at: 5227 5227
Size: 6721
HaeII r_GCGC'y
Cuts at: 1678 2081 5868 1678
Size: 403 3787 2531
Fragments arranged by size:
3787 2531 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3220 3252 3894 3926 5362
Size: 32 135 32 1648 32 642 32 1436
Cuts at: 5362 5394 5436 5468 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1648 1436 642 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3265 3982 4065 4311 669
Size: 988 281 1327 717 83 246 3079
Fragments arranged by size:
3079 1327 988 717 281 246 83
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6721
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KpnI G_GTAC'C
Cuts at: 4046 4046
Size: 6721
MfeI C'AATT_G
Cuts at: 4320 4320
Size: 6721
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3012 3396 5835 6019 1305
Size: 1707 384 2439 184 2007
Fragments arranged by size:
2439 2007 1707 384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2054 10
Size: 54 1425 343 222 4677
Fragments arranged by size:
4677 1425 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4679 5038 5197 518
Size: 648 3513 359 159 2042
Fragments arranged by size:
3513 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3211 3931 4137 4814 5962 6207 1606
Size: 1605 720 206 677 1148 245 2120
Fragments arranged by size:
2120 1605 1148 720 677 245 206
NaeI GCC'GGC
Cuts at: 2814 2814
Size: 6721
NcoI C'CATG_G
Cuts at: 513 1065 2004 3156 513
Size: 552 939 1152 4078
Fragments arranged by size:
4078 1152 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6721
NgoMIV G'CCGG_C
Cuts at: 2812 2812
Size: 6721
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6721
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 3788 4079 5624 1132
Size: 97 2559 291 1545 2229
Fragments arranged by size:
2559 2229 1545 291 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6721
PciI A'CATG_T
Cuts at: 5620 5620
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2823 3232 3620 1108
Size: 1715 409 388 4209
Fragments arranged by size:
4209 1715 409 388
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4790 4822 6303 6335 4790
Size: 32 1481 32 5176
Fragments arranged by size:
5176 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2279 3274 3492 2279
Size: 995 218 5508
Fragments arranged by size:
5508 995 218
PshAI GACnn'nnGTC
Cuts at: 1613 2722 1613
Size: 1109 5612
PsiI TTA'TAA
Cuts at: 4291 4291
Size: 6721
PstI C_TGCA'G
Cuts at: 830 2156 4073 830
Size: 1326 1917 3478
Fragments arranged by size:
3478 1917 1326
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
PvuII CAG'CTG
Cuts at: 3211 3931 3211
Size: 720 6001
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SanDI GG'GwC_CC
Cuts at: 2279 2279
Size: 6721
SbfI CC_TGCA'GG
Cuts at: 4073 4073
Size: 6721
ScaI AGT'ACT
Cuts at: 2858 4969 6681 2858
Size: 2111 1712 2898
Fragments arranged by size:
2898 2111 1712
SfcI C'TryA_G
Cuts at: 826 1045 2152 2727 4069 5204 5885 6076 826
Size: 219 1107 575 1342 1135 681 191 1471
Fragments arranged by size:
1471 1342 1135 1107 681 575 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6721
SmaI CCC'GGG
Cuts at: 1872 2022 4048 1872
Size: 150 2026 4545
Fragments arranged by size:
4545 2026 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4825 5726 5988 820
Size: 197 926 2882 901 262 1553
Fragments arranged by size:
2882 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4512 493
Size: 4019 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6721
SphI G_CATG'C
Cuts at: 1132 3788 4079 1132
Size: 2656 291 3774
Fragments arranged by size:
3774 2656 291
SspI AAT'ATT
Cuts at: 5 52 4645 5
Size: 47 4593 2081
Fragments arranged by size:
4593 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6721
StyI C'CwwG_G
Cuts at: 513 1065 2004 3156 3690 4160 513
Size: 552 939 1152 534 470 3074
Fragments arranged by size:
3074 1152 939 552 534 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2730 3015 3747 4748 4765 4918 5103
Size: 1255 1150 285 732 1001 17 153 185
Cuts at: 5103 5548 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1150 1001 732 445 285 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2856 3243 4967
Size: 274 80 33 51 1443 879 387 1724
Cuts at: 4967 6679 96
Size: 1712 138
Fragments arranged by size:
1724 1712 1443 879 387 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2383 2415 2647 2679 3484 3516 3543 3575 3653
Size: 32 232 32 805 32 27 32 78
Cuts at: 3653 3685 6681 6713 2383
Size: 32 2996 32 2391
Fragments arranged by size:
2996 2391 805 232 78 32 32 32
32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6721
XbaI T'CTAG_A
Cuts at: 4057 4057
Size: 6721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 2020 4046 1870
Size: 150 2026 4545
Fragments arranged by size:
4545 2026 150
XmnI GAAnn'nnTTC
Cuts at: 2765 4850 2765
Size: 2085 4636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI