Restriction map for
pFN21AB6225
(Circular) (Six-base) MAPSORT of: pFN21AB6225.seq Check: 6025 from: 1 to: 6718
pFN21AB6225 6718 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3526 3526
Size: 6718
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4528 278
Size: 53 83 186 936 2992 2468
Fragments arranged by size:
2992 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2703 3075 3225 4061 1937
Size: 766 372 150 836 4594
Fragments arranged by size:
4594 836 766 372 150
Acc65I G'GTAC_C
Cuts at: 2442 4039 2442
Size: 1597 5121
AclI AA'CG_TT
Cuts at: 1563 3989 4845 5218 1563
Size: 2426 856 373 3063
Fragments arranged by size:
3063 2426 856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2628 4781 6165 6433 1632
Size: 996 2153 1384 268 1917
Fragments arranged by size:
2153 1917 1384 996 268
AfeI AGC'GCT
Cuts at: 2354 2354
Size: 6718
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6521
AflIII A'CryG_T
Cuts at: 1203 5617 6594 1203
Size: 4414 977 1327
Fragments arranged by size:
4414 1327 977
AgeI A'CCGG_T
Cuts at: 5549 5549
Size: 6718
AhdI GACnn_n'nnGTC
Cuts at: 5447 5447
Size: 6718
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6718
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2568 1184
Size: 1384 5334
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3540 3572 3540
Size: 32 6686
AlwNI CAG_nnn'CTG
Cuts at: 2255 2439 2740 3364 4461 6033 2255
Size: 184 301 624 1097 1572 2940
Fragments arranged by size:
2940 1572 1097 624 301 184
ApaI G_GGCC'C
Cuts at: 2015 2382 2932 2015
Size: 367 550 5801
Fragments arranged by size:
5801 550 367
ApaLI G'TGCA_C
Cuts at: 4775 5931 4775
Size: 1156 5562
ApoI r'AATT_y
Cuts at: 1493 3980 4027 4253 4405 6514 1493
Size: 2487 47 226 152 2109 1697
Fragments arranged by size:
2487 2109 1697 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 5272 160
Size: 872 4240 1606
Fragments arranged by size:
4240 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 3090 3783 4043 1870
Size: 73 151 996 693 260 4545
Fragments arranged by size:
4545 996 693 260 151 73
AvrII C'CTAG_G
Cuts at: 3055 3055
Size: 6718
BamHI G'GATC_C
Cuts at: 4048 4415 4048
Size: 367 6351
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 2442 2917 3504 3755
Size: 325 217 627 320 335 475 587 251
Cuts at: 3755 4039 5494 618
Size: 284 1455 1842
Fragments arranged by size:
1842 1455 627 587 475 335 325 320
284 251 217
BanII G_rGCy'C
Cuts at: 1391 2015 2040 2382 2542 2912 2932 2975 4037
Size: 624 25 342 160 370 20 43 1062
Cuts at: 4037 1391
Size: 4072
Fragments arranged by size:
4072 1062 624 370 342 160 43 25
20
BbeI G_GCGC'C
Cuts at: 2921 3508 2921
Size: 587 6131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 3894 928
Size: 971 180 1815 3752
Fragments arranged by size:
3752 1815 971 180
BbvCI CC'TCA_GC
Cuts at: 2962 3643 2962
Size: 681 6037
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3524 3558 4909 4943 1903
Size: 34 50 34 1503 34 1351 34 3678
Fragments arranged by size:
3678 1503 1351 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4610 5820 4610
Size: 1210 5508
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6718
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2957 3282 5329
Size: 107 122 71 631 605 1285 325 2047
Cuts at: 5329 136
Size: 1525
Fragments arranged by size:
2047 1525 1285 631 605 325 122 107
71
BglII A'GATC_T
Cuts at: 6713 6713
Size: 6718
BlpI GC'TnA_GC
Cuts at: 2039 2849 3619 4135 2039
Size: 810 770 516 4622
Fragments arranged by size:
4622 810 770 516
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2112 2382 2932 3500 4779 5935 1790
Size: 225 97 270 550 568 1279 1156 2573
Fragments arranged by size:
2573 1279 1156 568 550 270 225 97
BmgBI CAC'GTC
Cuts at: 1318 3591 1318
Size: 2273 4445
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2766 3382 4449
Size: 416 53 83 399 24 1349 616 1067
Cuts at: 4449 5407 442
Size: 958 1753
Fragments arranged by size:
1753 1349 1067 958 616 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6718
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 6718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2664 2849 2959 2989 3278 3386 3431
Size: 815 610 185 110 30 289 108 45
Cuts at: 3431 3917 5378 1239
Size: 486 1461 2579
Fragments arranged by size:
2579 1461 815 610 486 289 185 110
108 45 30
Bpu10I CC'TnA_GC
Cuts at: 2962 3333 3643 2962
Size: 371 310 6037
Fragments arranged by size:
6037 371 310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3149 4843 5708 6006 3149
Size: 1694 865 298 3861
Fragments arranged by size:
3861 1694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2514 2879 3087 5381 882
Size: 617 1015 365 208 2294 2219
Fragments arranged by size:
2294 2219 1015 617 365 208
BsaAI yAC'GTr
Cuts at: 493 1204 2322 3532 4509 493
Size: 711 1118 1210 977 2702
Fragments arranged by size:
2702 1210 1118 977 711
BsaBI GATnn'nnATC
Cuts at: 6712 6712
Size: 6718
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2689 2918 3505 4525
Size: 53 83 186 936 1156 229 587 1020
Cuts at: 4525 4907 5542 275
Size: 382 635 1451
Fragments arranged by size:
1451 1156 1020 936 635 587 382 229
186 83 53
BsaWI w'CCGG_w
Cuts at: 2118 2445 2469 5151 5549 5823 5970 6381 2118
Size: 327 24 2682 398 274 147 411 2455
Fragments arranged by size:
2682 2455 411 398 327 274 147 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3366 3396 3540 3570 3620
Size: 30 165 30 1963 30 144 30 50
Cuts at: 3620 3650 3805 3835 1178
Size: 30 155 30 4061
Fragments arranged by size:
4061 1963 165 155 144 50 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 3808 1186
Size: 1336 1286 4096
Fragments arranged by size:
4096 1336 1286
BseYI C'CCAG_C
Cuts at: 2033 2214 2295 2355 2541 2595 2808 3124 3579
Size: 181 81 60 186 54 213 316 455
Cuts at: 3579 3632 3658 3791 5921 6417 2033
Size: 53 26 133 2130 496 2334
Fragments arranged by size:
2334 2130 496 455 316 213 186 181
133 81 60 54 53 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3554 1163
Size: 573 1818 4327
Fragments arranged by size:
4327 1818 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2952 3277 4168 4929 5957 1540
Size: 275 186 951 325 891 761 1028 2301
Fragments arranged by size:
2301 1028 951 891 761 325 275 186
BsiHKAI G_wGCw'C
Cuts at: 2685 2912 2975 3270 3444 4779 4864 5935 2685
Size: 227 63 295 174 1335 85 1071 3468
Fragments arranged by size:
3468 1335 1071 295 227 174 85 63
BsmI GAATG_Cn'
Cuts at: 2648 4229 4322 2648
Size: 1581 93 5044
Fragments arranged by size:
5044 1581 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3597 3597
Size: 6718
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2040 2112 2382 2542 2685 2912
Size: 399 225 25 72 270 160 143 227
Cuts at: 2912 2932 2975 3270 3444 3500 4037 4779 4864
Size: 20 43 295 174 56 537 742 85
Cuts at: 4864 5935 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 537 399 295 270 227
225 174 160 143 85 72 56 43
25 20
BspEI T'CCGG_A
Cuts at: 2118 6381 2118
Size: 4263 2455
BspHI T'CATG_A
Cuts at: 4607 6337 4607
Size: 1730 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2560 2880 3526 4073 844
Size: 1716 320 646 547 3489
Fragments arranged by size:
3489 1716 646 547 320
BsrBI CCG'CTC
Cuts at: 1394 1823 2394 3618 4605 1394
Size: 429 571 1224 987 3507
Fragments arranged by size:
3507 1224 987 571 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2188 2403 5213 5387 65
Size: 1183 940 215 2810 174 1396
Fragments arranged by size:
2810 1396 1183 940 215 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2914 2951 5362 5549 1700
Size: 110 1104 37 2411 187 2869
Fragments arranged by size:
2869 2411 1104 187 110 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6718
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6718
BssSI C'ACGA_G
Cuts at: 3543 4778 5790 3543
Size: 1235 1012 4471
Fragments arranged by size:
4471 1235 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6718
BstBI TT'CG_AA
Cuts at: 5522 5522
Size: 6718
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6718
BstXI CCAn_nnnn'nTGG
Cuts at: 3676 3752 3676
Size: 76 6642
BstYI r'GATC_y
Cuts at: 1972 2283 3327 4048 4415 4800 4817 6269 6355
Size: 311 1044 721 367 385 17 1452 86
Cuts at: 6355 6367 6713 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1044 721 385 367 346 311
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2382 3271 513
Size: 552 1317 889 3960
Fragments arranged by size:
3960 1317 889 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3875 487
Size: 683 56 2649 3330
Fragments arranged by size:
3330 2649 683 56
BtsI GCAGTG_nn'
Cuts at: 3941 4234 5028 5048 3941
Size: 293 794 20 5611
Fragments arranged by size:
5611 794 293 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6683
DraI TTT'AAA
Cuts at: 4022 4022
Size: 6718
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2722 5725 809
Size: 507 1406 3003 1802
Fragments arranged by size:
3003 1802 1406 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2246 2841
Size: 54 1425 50 275 18 232 184 595
Cuts at: 2841 2949 3274 4165 5054 6683 8
Size: 108 325 891 889 1629 43
Fragments arranged by size:
1629 1425 891 889 595 325 275 232
184 108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2949 3274 4165 1537
Size: 275 1137 325 891 4090
Fragments arranged by size:
4090 1137 891 325 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2604 2715 3132 3140 4648 1353
Size: 1251 111 417 8 1508 3423
Fragments arranged by size:
3423 1508 1251 417 111 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2995 5305 5679 5825 387
Size: 1064 138 1406 2310 374 146 1280
Fragments arranged by size:
2310 1406 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2910 2973 2910
Size: 63 6655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2628 2664 2849 2959 2989 3278
Size: 393 422 574 36 185 110 30 289
Cuts at: 3278 3386 3431 3917 4781 5378 6165 6433 1239
Size: 108 45 486 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 597 574 486 422 393
289 268 185 110 108 45 36 30
EcoO109I rG'GnC_Cy
Cuts at: 2011 2252 2869 2929 3406 3788 3813 3876 3972
Size: 241 617 60 477 382 25 63 96
Cuts at: 3972 2011
Size: 4757
Fragments arranged by size:
4757 617 477 382 241 96 63 60
25
EcoRI G'AATT_C
Cuts at: 4027 4405 4027
Size: 378 6340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 6718
FseI GG_CCGG'CC
Cuts at: 2955 2955
Size: 6718
FspI TGC'GCA
Cuts at: 3653 5224 3653
Size: 1571 5147
HaeII r_GCGC'y
Cuts at: 1678 2356 2856 2921 3508 5865 1678
Size: 678 500 65 587 2357 2531
Fragments arranged by size:
2531 2357 678 587 500 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3429 3461 3733 3765 3805
Size: 32 135 32 1857 32 272 32 40
Cuts at: 3805 3837 5359 5391 5433 5465 1373
Size: 32 1522 32 42 32 2626
Fragments arranged by size:
2626 1857 1522 272 135 42 40 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 4062 4308 669
Size: 988 281 487 1637 246 3079
Fragments arranged by size:
3079 1637 988 487 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6718
HpaI GTT'AAC
Cuts at: 4308 4308
Size: 6718
KasI G'GCGC_C
Cuts at: 2917 3504 2917
Size: 587 6131
KpnI G_GTAC'C
Cuts at: 2446 4043 2446
Size: 1597 5121
MfeI C'AATT_G
Cuts at: 2880 4317 2880
Size: 1437 5281
MluI A'CGCG_T
Cuts at: 6594 6594
Size: 6718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2157 3041 5832 6016 1305
Size: 852 884 2791 184 2007
Fragments arranged by size:
2791 2007 884 852 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 2248 10
Size: 54 1425 343 232 184 4480
Fragments arranged by size:
4480 1425 343 232 184 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2819 4676 5035 5194 518
Size: 648 1653 1857 359 159 2042
Fragments arranged by size:
2042 1857 1653 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2491 2779 2941 3608 3662 4134 4811 5959
Size: 885 288 162 667 54 472 677 1148
Cuts at: 5959 6204 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 885 677 667 472 288 245
162 54
NaeI GCC'GGC
Cuts at: 2916 2953 2916
Size: 37 6681
NarI GG'CG_CC
Cuts at: 2918 3505 2918
Size: 587 6131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6166
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6718
NgoMIV G'CCGG_C
Cuts at: 2914 2951 2914
Size: 37 6681
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6718
NotI GC'GGCC_GC
Cuts at: 4165 4165
Size: 6718
NspI r_CATG'y
Cuts at: 1132 1229 2401 2990 4076 5621 1132
Size: 97 1172 589 1086 1545 2229
Fragments arranged by size:
2229 1545 1172 1086 589 97
PasI CC'CwG_GG
Cuts at: 1348 2255 1348
Size: 907 5811
PciI A'CATG_T
Cuts at: 5617 5617
Size: 6718
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2255 2439 3031 3364 3802 1108
Size: 1147 184 592 333 438 4024
Fragments arranged by size:
4024 1147 592 438 333 184
PfoI T'CCnGG_A
Cuts at: 2726 3965 2726
Size: 1239 5479
PmeI GTTT'AAAC
Cuts at: 4022 4022
Size: 6718
PmlI CAC'GTG
Cuts at: 2322 2322
Size: 6718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3540 3572 4787 4819 6300 6332 3540
Size: 32 1215 32 1481 32 3926
Fragments arranged by size:
3926 1481 1215 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2869 3406 3788 3813 3972 2869
Size: 537 382 25 159 5615
Fragments arranged by size:
5615 537 382 159 25
PshAI GACnn'nnGTC
Cuts at: 1613 2896 1613
Size: 1283 5435
PsiI TTA'TAA
Cuts at: 4288 4288
Size: 6718
PspOMI G'GGCC_C
Cuts at: 2011 2378 2928 2011
Size: 367 550 5801
Fragments arranged by size:
5801 550 367
PstI C_TGCA'G
Cuts at: 830 2667 2745 3210 4070 830
Size: 1837 78 465 860 3478
Fragments arranged by size:
3478 1837 860 465 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6718
PvuII CAG'CTG
Cuts at: 2491 3662 2491
Size: 1171 5547
SacI G_AGCT'C
Cuts at: 2912 2975 2912
Size: 63 6655
SalI G'TCGA_C
Cuts at: 1936 4060 1936
Size: 2124 4594
SbfI CC_TGCA'GG
Cuts at: 4070 4070
Size: 6718
ScaI AGT'ACT
Cuts at: 4966 6678 4966
Size: 1712 5006
SexAI A'CCwGG_T
Cuts at: 3357 3357
Size: 6718
SfcI C'TryA_G
Cuts at: 826 1045 2635 2663 2741 3206 4066 5201 5882
Size: 219 1590 28 78 465 860 1135 681
Cuts at: 5882 6073 826
Size: 191 1471
Fragments arranged by size:
1590 1471 1135 860 681 465 219 191
78 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 2957 2957
Size: 6718
SfoI GGC'GCC
Cuts at: 2919 3506 2919
Size: 587 6131
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
SgrAI Cr'CCGG_yG
Cuts at: 1700 2914 1700
Size: 1214 5504
SmaI CCC'GGG
Cuts at: 1872 2096 4045 1872
Size: 224 1949 4545
Fragments arranged by size:
4545 1949 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3164 3783 4822 5723 5985 820
Size: 197 926 1221 619 1039 901 262 1553
Fragments arranged by size:
1553 1221 1039 926 901 619 262 197
SnaBI TAC'GTA
Cuts at: 493 4509 493
Size: 4016 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6718
SphI G_CATG'C
Cuts at: 1132 2401 4076 1132
Size: 1269 1675 3774
Fragments arranged by size:
3774 1675 1269
SspI AAT'ATT
Cuts at: 5 52 4642 5
Size: 47 4590 2081
Fragments arranged by size:
4590 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6718
StyI C'CwwG_G
Cuts at: 513 1065 2328 3055 3192 3508 4157 513
Size: 552 1263 727 137 316 649 3074
Fragments arranged by size:
3074 1263 727 649 552 316 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 2530 3737 3947 4745 4762 4915
Size: 1255 738 212 1207 210 798 17 153
Cuts at: 4915 5100 5545 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1207 798 738 445 212 210
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3390 3870 4964
Size: 274 80 33 51 1443 1413 480 1094
Cuts at: 4964 6676 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1413 1094 480 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 2216 2248 3366 3398 3620 3652 3748
Size: 32 127 32 1118 32 222 32 96
Cuts at: 3748 3780 6678 6710 2057
Size: 32 2898 32 2065
Fragments arranged by size:
2898 2065 1118 222 127 96 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2708 3071 3221 1316
Size: 1392 363 150 4813
Fragments arranged by size:
4813 1392 363 150
XbaI T'CTAG_A
Cuts at: 4054 4054
Size: 6718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2776 3799 2776
Size: 1023 5695
XhoI C'TCGA_G
Cuts at: 1943 3783 1943
Size: 1840 4878
XmaI C'CCGG_G
Cuts at: 1870 2094 4043 1870
Size: 224 1949 4545
Fragments arranged by size:
4545 1949 224
XmnI GAAnn'nnTTC
Cuts at: 4847 4847
Size: 6718
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4526 276
Size: 53 83 186 936 2992 2468
Fragments arranged by size:
2992 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SapI SrfI SwaI