Restriction map for
pFN21AB6223
(Circular) (Six-base) MAPSORT of: pFN21AB6223.seq Check: 9746 from: 1 to: 6715
pFN21AB6223 6715 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2989 4525 278
Size: 53 83 186 936 1453 1536 2468
Fragments arranged by size:
2468 1536 1453 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4058 1937
Size: 2121 4594
Acc65I G'GTAC_C
Cuts at: 4036 4036
Size: 6715
AclI AA'CG_TT
Cuts at: 1563 4842 5215 1563
Size: 3279 373 3063
Fragments arranged by size:
3279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2416 2763 4023 4778 6162 6430 1632
Size: 784 347 1260 755 1384 268 1917
Fragments arranged by size:
1917 1384 1260 784 755 347 268
AfeI AGC'GCT
Cuts at: 3632 3632
Size: 6715
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6518
AflIII A'CryG_T
Cuts at: 1203 2194 5614 6591 1203
Size: 991 3420 977 1327
Fragments arranged by size:
3420 1327 991 977
AgeI A'CCGG_T
Cuts at: 5546 5546
Size: 6715
AhdI GACnn_n'nnGTC
Cuts at: 5444 5444
Size: 6715
AleI CACnn'nnGTG
Cuts at: 1166 2313 3414 1166
Size: 1147 1101 4467
Fragments arranged by size:
4467 1147 1101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6715
AlwNI CAG_nnn'CTG
Cuts at: 4458 6030 4458
Size: 1572 5143
ApaLI G'TGCA_C
Cuts at: 4772 5928 4772
Size: 1156 5559
ApoI r'AATT_y
Cuts at: 1493 2817 2966 3321 3464 3573 4024 4250 4402
Size: 1324 149 355 143 109 451 226 152
Cuts at: 4402 6511 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1324 451 355 226 152 149
143 109
AseI AT'TA_AT
Cuts at: 160 1032 5269 160
Size: 872 4237 1606
Fragments arranged by size:
4237 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
AvaI C'yCGr_G
Cuts at: 1870 1943 4040 1870
Size: 73 2097 4545
Fragments arranged by size:
4545 2097 73
BamHI G'GATC_C
Cuts at: 4045 4412 4045
Size: 367 6348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4036 5491 618
Size: 325 217 627 2249 1455 1842
Fragments arranged by size:
2249 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 2139 2481 3803 4034 1391
Size: 660 88 342 1322 231 4072
Fragments arranged by size:
4072 1322 660 342 231 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5744
BbvCI CC'TCA_GC
Cuts at: 2069 2069
Size: 6715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4906 4940 1903
Size: 34 50 34 2885 34 3678
Fragments arranged by size:
3678 2885 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4607 5817 4607
Size: 1210 5505
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6715
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5326 136
Size: 107 122 71 631 605 3654 1525
Fragments arranged by size:
3654 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6710 6710
Size: 6715
BlpI GC'TnA_GC
Cuts at: 2364 4132 2364
Size: 1768 4947
Bme1580I G_kGCm'C
Cuts at: 1790 4776 5932 1790
Size: 2986 1156 2573
Fragments arranged by size:
2986 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6715
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2330 2598 4446
Size: 416 53 83 399 24 913 268 1848
Cuts at: 4446 5404 442
Size: 958 1753
Fragments arranged by size:
1848 1753 958 913 416 399 268 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3723 1056
Size: 2667 4048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2931 3015 3099 3183 3267 3519 3687
Size: 962 730 84 84 84 84 252 168
Cuts at: 3687 3771 3855 3939 5375 1239
Size: 84 84 84 1436 2579
Fragments arranged by size:
2579 1436 962 730 252 168 84 84
84 84 84 84 84
Bpu10I CC'TnA_GC
Cuts at: 2069 2082 2069
Size: 13 6702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4840 5705 6003 4840
Size: 865 298 5552
Fragments arranged by size:
5552 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5378 882
Size: 617 3879 2219
Fragments arranged by size:
3879 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4506 493
Size: 711 3302 2702
Fragments arranged by size:
3302 2702 711
BsaBI GATnn'nnATC
Cuts at: 6709 6709
Size: 6715
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2986 4522 4904 5539
Size: 53 83 186 936 1453 1536 382 635
Cuts at: 5539 275
Size: 1451
Fragments arranged by size:
1536 1453 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3567 3651 5148 5546 5820 5967 6378 3567
Size: 84 1497 398 274 147 411 3904
Fragments arranged by size:
3904 1497 411 398 274 147 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6490
Fragments arranged by size:
6490 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 3012 1186
Size: 1441 385 4889
Fragments arranged by size:
4889 1441 385
BseYI C'CCAG_C
Cuts at: 2566 5918 6414 2566
Size: 3352 496 2867
Fragments arranged by size:
3352 2867 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3286 3454 3622 3874 1163
Size: 573 1550 168 168 252 4004
Fragments arranged by size:
4004 1550 573 252 168 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2023 4165 4926 5954 1540
Size: 275 186 18 4 2142 761 1028 2301
Fragments arranged by size:
2301 2142 1028 761 275 186 18 4
BsiHKAI G_wGCw'C
Cuts at: 2051 2139 2370 3803 4776 4861 5932 2051
Size: 88 231 1433 973 85 1071 2834
Fragments arranged by size:
2834 1433 1071 973 231 88 85
BsmI GAATG_Cn'
Cuts at: 2933 3353 3405 3521 3941 4226 4319 2933
Size: 420 52 116 420 285 93 5329
Fragments arranged by size:
5329 420 420 285 116 93 52
BsmBI CGTCTCn'nnnn_
Cuts at: 2977 2977
Size: 6715
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2139 2370 2481 3803 4034 4776
Size: 399 261 88 231 111 1322 231 742
Cuts at: 4776 4861 5932 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1322 1071 742 399 261 231 231
111 88 85
BspEI T'CCGG_A
Cuts at: 6378 6378
Size: 6715
BspHI T'CATG_A
Cuts at: 3007 4604 6334 3007
Size: 1597 1730 3388
Fragments arranged by size:
3388 1730 1597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4070 844
Size: 3226 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 4602 1394
Size: 429 205 2574 3507
Fragments arranged by size:
3507 2574 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 5210 5384 65
Size: 1183 3962 174 1396
Fragments arranged by size:
3962 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2516 5359 5546 1700
Size: 110 706 2843 187 2869
Fragments arranged by size:
2869 2843 706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6715
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6715
BssSI C'ACGA_G
Cuts at: 4775 5787 4775
Size: 1012 5703
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2877 794
Size: 2083 4632
BstBI TT'CG_AA
Cuts at: 5519 5519
Size: 6715
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6715
BstYI r'GATC_y
Cuts at: 1972 4045 4412 4797 4814 6266 6352 6364 6710
Size: 2073 367 385 17 1452 86 12 346
Cuts at: 6710 1972
Size: 1977
Fragments arranged by size:
2073 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2042 2218 2574 513
Size: 552 977 176 356 4654
Fragments arranged by size:
4654 977 552 356 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2184 487
Size: 683 56 958 5018
Fragments arranged by size:
5018 958 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3274 3442 3526 3610 3946 4231 5025 5045
Size: 336 168 84 84 336 285 794 20
Cuts at: 5045 2938
Size: 4608
Fragments arranged by size:
4608 794 336 336 285 168 84 84
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2111 2146 529
Size: 35 1547 35 5098
Fragments arranged by size:
5098 1547 35 35
DraI TTT'AAA
Cuts at: 4019 4019
Size: 6715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5722 809
Size: 507 4406 1802
Fragments arranged by size:
4406 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2020 2106 4162
Size: 54 1425 50 275 18 190 86 2056
Cuts at: 4162 5051 6680 8
Size: 889 1629 43
Fragments arranged by size:
2056 1629 1425 889 275 190 86 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2020 4162 1537
Size: 275 208 2142 4090
Fragments arranged by size:
4090 2142 275 208
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2469 3229 4645 1353
Size: 768 348 760 1416 3423
Fragments arranged by size:
3423 1416 768 760 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5302 5676 5822 387
Size: 1064 138 3713 374 146 1280
Fragments arranged by size:
3713 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2049 2137 3801 2049
Size: 88 1664 4963
Fragments arranged by size:
4963 1664 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2416 2763 2931 3015 3099 3183
Size: 393 569 215 347 168 84 84 84
Cuts at: 3183 3267 3519 3687 3771 3855 3939 4023 4778
Size: 84 252 168 84 84 84 84 755
Cuts at: 4778 5375 6162 6430 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 755 597 569 393 347 268
252 215 168 168 84 84 84 84
84 84 84 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2485 2485
Size: 6715
EcoRI G'AATT_C
Cuts at: 2966 3321 3573 4024 4402 2966
Size: 355 252 451 378 5279
Fragments arranged by size:
5279 451 378 355 252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2768 2416
Size: 352 6363
FspI TGC'GCA
Cuts at: 5221 5221
Size: 6715
HaeII r_GCGC'y
Cuts at: 1678 3634 5862 1678
Size: 1956 2228 2531
Fragments arranged by size:
2531 2228 1956
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5356 5388 5430 5462 1373
Size: 32 135 32 3784 32 42 32 2626
Fragments arranged by size:
3784 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4059 4305 669
Size: 988 281 2121 246 3079
Fragments arranged by size:
3079 2121 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2694 748
Size: 1946 4769
HpaI GTT'AAC
Cuts at: 4305 4305
Size: 6715
KpnI G_GTAC'C
Cuts at: 4040 4040
Size: 6715
MfeI C'AATT_G
Cuts at: 4314 4314
Size: 6715
MluI A'CGCG_T
Cuts at: 6591 6591
Size: 6715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2611 5829 6013 1305
Size: 1306 3218 184 2007
Fragments arranged by size:
3218 2007 1306 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 10
Size: 54 1425 343 276 4617
Fragments arranged by size:
4617 1425 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 2414 2466 2730 3012 3414 4673
Size: 648 1147 101 52 264 282 402 1259
Cuts at: 4673 5032 5191 518
Size: 359 159 2042
Fragments arranged by size:
2042 1259 1147 648 402 359 282 264
159 101 52
MspA1I CmG'CkG
Cuts at: 1606 4131 4808 5956 6201 1606
Size: 2525 677 1148 245 2120
Fragments arranged by size:
2525 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2218 513
Size: 552 1153 5010
Fragments arranged by size:
5010 1153 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6715
NheI G'CTAG_C
Cuts at: 1052 3719 1052
Size: 2667 4048
NotI GC'GGCC_GC
Cuts at: 4162 4162
Size: 6715
NspI r_CATG'y
Cuts at: 1132 1229 2198 4073 5618 1132
Size: 97 969 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 969 97
PasI CC'CwG_GG
Cuts at: 1348 2206 1348
Size: 858 5857
PciI A'CATG_T
Cuts at: 2194 5614 2194
Size: 3420 3295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2224 1108
Size: 1116 5599
PmeI GTTT'AAAC
Cuts at: 4019 4019
Size: 6715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2754 4784 4816 6297 6329 2722
Size: 32 2030 32 1481 32 3108
Fragments arranged by size:
3108 2030 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6715
PsiI TTA'TAA
Cuts at: 4285 4285
Size: 6715
PstI C_TGCA'G
Cuts at: 830 2525 4067 830
Size: 1695 1542 3478
Fragments arranged by size:
3478 1695 1542
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6715
SacI G_AGCT'C
Cuts at: 2051 2139 3803 2051
Size: 88 1664 4963
Fragments arranged by size:
4963 1664 88
SalI G'TCGA_C
Cuts at: 1936 4057 1936
Size: 2121 4594
SapI GCTCTTCn'nnn_
Cuts at: 2469 2469
Size: 6715
SbfI CC_TGCA'GG
Cuts at: 4067 4067
Size: 6715
ScaI AGT'ACT
Cuts at: 4963 6675 4963
Size: 1712 5003
SfcI C'TryA_G
Cuts at: 826 1045 2521 4063 5198 5879 6070 826
Size: 219 1476 1542 1135 681 191 1471
Fragments arranged by size:
1542 1476 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6715
SmaI CCC'GGG
Cuts at: 1872 4042 1872
Size: 2170 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4819 5720 5982 820
Size: 197 926 2876 901 262 1553
Fragments arranged by size:
2876 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4506 493
Size: 4013 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6715
SphI G_CATG'C
Cuts at: 1132 4073 1132
Size: 2941 3774
SspI AAT'ATT
Cuts at: 5 52 4639 5
Size: 47 4587 2081
Fragments arranged by size:
4587 2081 47
StuI AGG'CCT
Cuts at: 1517 2561 2920 3088 3172 3424 3676 3760 3928
Size: 1044 359 168 84 252 252 84 168
Cuts at: 3928 4012 1517
Size: 84 4220
Fragments arranged by size:
4220 1044 359 252 252 168 168 84
84 84
StyI C'CwwG_G
Cuts at: 513 1065 2218 4154 513
Size: 552 1153 1936 3074
Fragments arranged by size:
3074 1936 1153 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2308 2312 4742 4759 4912 5097 5542
Size: 1255 728 4 2430 17 153 185 445
Cuts at: 5542 325
Size: 1498
Fragments arranged by size:
2430 1498 1255 728 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 4961 6673
Size: 274 80 33 51 1443 186 2798 1712
Cuts at: 6673 96
Size: 138
Fragments arranged by size:
2798 1712 1443 274 186 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6675 6707 6675
Size: 32 6683
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6715
XbaI T'CTAG_A
Cuts at: 4051 4051
Size: 6715
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6715
XmaI C'CCGG_G
Cuts at: 1870 4040 1870
Size: 2170 4545
XmnI GAAnn'nnTTC
Cuts at: 2543 2695 2780 2970 3306 4844 2543
Size: 152 85 190 336 1538 4414
Fragments arranged by size:
4414 1538 336 190 152 85
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2987 4523 276
Size: 53 83 186 936 1453 1536 2468
Fragments arranged by size:
2468 1536 1453 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI