Restriction map for
pFN21AB6220
(Circular) (Six-base) MAPSORT of: pFN21AB6220.seq Check: 7756 from: 1 to: 6907
pFN21AB6220 6907 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2539 3207 2539
Size: 668 6239
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4717 278
Size: 53 83 186 936 3181 2468
Fragments arranged by size:
3181 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4250 1937
Size: 2313 4594
Acc65I G'GTAC_C
Cuts at: 4228 4228
Size: 6907
AclI AA'CG_TT
Cuts at: 1563 5034 5407 1563
Size: 3471 373 3063
Fragments arranged by size:
3471 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2145 3166 3805 4970 6354 6622 1632
Size: 513 1021 639 1165 1384 268 1917
Fragments arranged by size:
1917 1384 1165 1021 639 513 268
AfeI AGC'GCT
Cuts at: 3359 3566 3662 3818 3359
Size: 207 96 156 6448
Fragments arranged by size:
6448 207 156 96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6710
AflIII A'CryG_T
Cuts at: 1203 3842 5806 6783 1203
Size: 2639 1964 977 1327
Fragments arranged by size:
2639 1964 1327 977
AgeI A'CCGG_T
Cuts at: 5738 5738
Size: 6907
AhdI GACnn_n'nnGTC
Cuts at: 5636 5636
Size: 6907
AleI CACnn'nnGTG
Cuts at: 1166 3141 3885 4032 1166
Size: 1975 744 147 4041
Fragments arranged by size:
4041 1975 744 147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3131 1184
Size: 1947 4960
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3306 3338 3221
Size: 32 53 32 6790
Fragments arranged by size:
6790 53 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2123 2221 2254 2281 2498 4102 4650 6222 2123
Size: 98 33 27 217 1604 548 1572 2808
Fragments arranged by size:
2808 1604 1572 548 217 98 33 27
ApaI G_GGCC'C
Cuts at: 2178 2505 2740 2987 3407 2178
Size: 327 235 247 420 5678
Fragments arranged by size:
5678 420 327 247 235
ApaLI G'TGCA_C
Cuts at: 3302 3605 4964 6120 3302
Size: 303 1359 1156 4089
Fragments arranged by size:
4089 1359 1156 303
ApoI r'AATT_y
Cuts at: 1493 4216 4442 4594 6703 1493
Size: 2723 226 152 2109 1697
Fragments arranged by size:
2723 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3493 3493
Size: 6907
AseI AT'TA_AT
Cuts at: 160 1032 5461 160
Size: 872 4429 1606
Fragments arranged by size:
4429 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6907
AvaI C'yCGr_G
Cuts at: 1870 1943 2339 3400 3440 3803 3971 4232 1870
Size: 73 396 1061 40 363 168 261 4545
Fragments arranged by size:
4545 1061 396 363 261 168 73 40
AvrII C'CTAG_G
Cuts at: 2516 2516
Size: 6907
BamHI G'GATC_C
Cuts at: 2965 4237 4604 2965
Size: 1272 367 5268
Fragments arranged by size:
5268 1272 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3123 4228 5683 618
Size: 325 217 627 1336 1105 1455 1842
Fragments arranged by size:
1842 1455 1336 1105 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2027 2178 2451 2505 2740 2987 3407 4226
Size: 636 151 273 54 235 247 420 819
Cuts at: 4226 1391
Size: 4072
Fragments arranged by size:
4072 819 636 420 273 247 235 151
54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5936
BbvCI CC'TCA_GC
Cuts at: 3119 3119
Size: 6907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2330 2364 5098 5132 1903
Size: 34 393 34 2734 34 3678
Fragments arranged by size:
3678 2734 393 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4799 6009 4799
Size: 1210 5697
BclI T'GATC_A
Cuts at: 1548 2994 1548
Size: 1446 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2871 5518 136
Size: 107 122 71 631 605 1199 2647 1525
Fragments arranged by size:
2647 1525 1199 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2679 6902 2679
Size: 4223 2684
BlpI GC'TnA_GC
Cuts at: 3414 4324 3414
Size: 910 5997
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2505 2740 2987 3306 3407 3609 4968
Size: 388 327 235 247 319 101 202 1359
Cuts at: 4968 6124 1790
Size: 1156 2573
Fragments arranged by size:
2573 1359 1156 388 327 319 247 235
202 101
BmgBI CAC'GTC
Cuts at: 1318 2839 1318
Size: 1521 5386
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2549 2709 3057
Size: 416 53 83 399 24 1132 160 348
Cuts at: 3057 4638 5596 442
Size: 1581 958 1753
Fragments arranged by size:
1753 1581 1132 958 416 399 348 160
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6907
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2731 2254
Size: 477 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2291 2437 2475 2612 3527 5567 1239
Size: 1037 15 146 38 137 915 2040 2579
Fragments arranged by size:
2579 2040 1037 915 146 137 38 15
Bpu10I CC'TnA_GC
Cuts at: 3119 3119
Size: 6907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3818 5032 5897 6195 3818
Size: 1214 865 298 4530
Fragments arranged by size:
4530 1214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3919 5570 882
Size: 617 2420 1651 2219
Fragments arranged by size:
2420 2219 1651 617
BsaAI yAC'GTr
Cuts at: 493 1204 4698 493
Size: 711 3494 2702
Fragments arranged by size:
3494 2702 711
BsaBI GATnn'nnATC
Cuts at: 6901 6901
Size: 6907
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4714 5096 5731 275
Size: 53 83 186 936 3181 382 635 1451
Fragments arranged by size:
3181 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2145 5340 5738 6012 6159 6570 2145
Size: 3195 398 274 147 411 2482
Fragments arranged by size:
3195 2482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2467 2497 2619 2649 3221
Size: 30 165 30 1064 30 122 30 572
Cuts at: 3221 3251 1178
Size: 30 4834
Fragments arranged by size:
4834 1064 572 165 122 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2105 2177 2357 2573 2660 2953 3242
Size: 916 3 72 180 216 87 293 289
Cuts at: 3242 1186
Size: 4851
Fragments arranged by size:
4851 916 293 289 216 180 87 72
3
BseYI C'CCAG_C
Cuts at: 2020 2193 2756 2927 3041 4011 6110 6606 2020
Size: 173 563 171 114 970 2099 496 2321
Fragments arranged by size:
2321 2099 970 563 496 173 171 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2115 2336 2435 3181 3484 1163
Size: 573 379 221 99 746 303 4586
Fragments arranged by size:
4586 746 573 379 303 221 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3175 3236 3574 4357 5118
Size: 275 186 1072 102 61 338 783 761
Cuts at: 5118 6146 1540
Size: 1028 2301
Fragments arranged by size:
2301 1072 1028 783 761 338 275 186
102 61
BsiHKAI G_wGCw'C
Cuts at: 2041 2730 3306 3513 3609 3933 4169 4968 5053
Size: 689 576 207 96 324 236 799 85
Cuts at: 5053 6124 2041
Size: 1071 2824
Fragments arranged by size:
2824 1071 799 689 576 324 236 207
96 85
BsmI GAATG_Cn'
Cuts at: 4418 4511 4418
Size: 93 6814
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2041 2178 2451 2505 2730 2740
Size: 399 237 14 137 273 54 225 10
Cuts at: 2740 2987 3306 3407 3513 3609 3933 4169 4226
Size: 247 319 101 106 96 324 236 57
Cuts at: 4226 4968 5053 6124 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 319 273 247
237 236 225 137 106 101 96 85
57 54 14 10
BspEI T'CCGG_A
Cuts at: 6570 6570
Size: 6907
BspHI T'CATG_A
Cuts at: 4796 6526 4796
Size: 1730 5177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2539 2798 3123 3207 3901 4262 844
Size: 1695 259 325 84 694 361 3489
Fragments arranged by size:
3489 1695 694 361 325 259 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3232 3579 3619 3703 4794 1394
Size: 429 1409 347 40 84 1091 3507
Fragments arranged by size:
3507 1409 1091 429 347 84 40
BsrDI GCAATG_nn'
Cuts at: 65 1248 5402 5576 65
Size: 1183 4154 174 1396
Fragments arranged by size:
4154 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3597 3681 3863 4017 5551 5738 1700
Size: 110 1787 84 182 154 1534 187 2869
Fragments arranged by size:
2869 1787 1534 187 182 154 110 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6907
BssHII G'CGCG_C
Cuts at: 1925 3276 3278 3493 1925
Size: 1351 2 215 5339
Fragments arranged by size:
5339 1351 215 2
BssSI C'ACGA_G
Cuts at: 4967 5979 4967
Size: 1012 5895
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3168 3202 3471 4102 794
Size: 2374 34 269 631 3599
Fragments arranged by size:
3599 2374 631 269 34
BstBI TT'CG_AA
Cuts at: 5711 5711
Size: 6907
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6907
BstXI CCAn_nnnn'nTGG
Cuts at: 2660 4145 2660
Size: 1485 5422
BstYI r'GATC_y
Cuts at: 1972 2301 2679 2877 2965 3132 4237 4604 4989
Size: 329 378 198 88 167 1105 367 385
Cuts at: 4989 5006 6458 6544 6556 6902 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1105 385 378 367 346 329
198 167 88 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2643 2706 2643
Size: 63 6844
BtgI C'CryG_G
Cuts at: 513 1065 2955 3388 513
Size: 552 1890 433 4032
Fragments arranged by size:
4032 1890 552 433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3093 487
Size: 683 56 1867 4301
Fragments arranged by size:
4301 1867 683 56
BtsI GCAGTG_nn'
Cuts at: 2288 3880 4423 5217 5237 2288
Size: 1592 543 794 20 3958
Fragments arranged by size:
3958 1592 794 543 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6872
DraI TTT'AAA
Cuts at: 4211 4211
Size: 6907
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5914 809
Size: 507 4598 1802
Fragments arranged by size:
4598 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2327 2872 3070
Size: 54 1425 50 275 18 497 545 198
Cuts at: 3070 3172 3233 3256 3340 3571 3643 3811 3979
Size: 102 61 23 84 231 72 168 168
Cuts at: 3979 4147 4354 5243 6872 8
Size: 168 207 889 1629 43
Fragments arranged by size:
1629 1425 889 545 497 275 231 207
198 168 168 168 102 84 72 61
54 50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 3172 3233 3571 4354 1537
Size: 275 1258 102 61 338 783 4090
Fragments arranged by size:
4090 1258 783 338 275 102 61
EarI CTCTTCn'nnn_
Cuts at: 1353 3282 4837 1353
Size: 1929 1555 3423
Fragments arranged by size:
3423 1929 1555
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2400 3728 4064 5494 5868 6014
Size: 1064 138 811 1328 336 1430 374 146
Cuts at: 6014 387
Size: 1280
Fragments arranged by size:
1430 1328 1280 1064 811 374 336 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2276 2291 2437 2475 2612 3166
Size: 393 513 131 15 146 38 137 554
Cuts at: 3166 3527 3805 4970 5567 6354 6622 1239
Size: 361 278 1165 597 787 268 1524
Fragments arranged by size:
1524 1165 787 597 554 513 393 361
278 268 146 137 131 38 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 2719 2642
Size: 77 6830
EcoO109I rG'GnC_Cy
Cuts at: 2057 2113 2120 2174 2175 2185 2428 2711 2736
Size: 56 7 54 1 10 243 283 25
Cuts at: 2736 2737 2848 2950 2984 3404 2057
Size: 1 111 102 34 420 5560
Fragments arranged by size:
5560 420 283 243 111 102 56 54
34 25 10 7 1 1
EcoRI G'AATT_C
Cuts at: 4216 4594 4216
Size: 378 6529
FspI TGC'GCA
Cuts at: 2910 3272 4106 5413 2910
Size: 362 834 1307 4404
Fragments arranged by size:
4404 1307 834 362
HaeII r_GCGC'y
Cuts at: 1678 3013 3361 3414 3568 3664 3820 3832 4203
Size: 1335 348 53 154 96 156 12 371
Cuts at: 4203 6054 1678
Size: 1851 2531
Fragments arranged by size:
2531 1851 1335 371 348 156 154 96
53 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 5548 5580 5622
Size: 32 135 32 845 32 3099 32 42
Cuts at: 5622 5654 1373
Size: 32 2626
Fragments arranged by size:
3099 2626 845 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4251 4497 669
Size: 988 281 2313 246 3079
Fragments arranged by size:
3079 2313 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6907
HpaI GTT'AAC
Cuts at: 4497 4497
Size: 6907
KpnI G_GTAC'C
Cuts at: 4232 4232
Size: 6907
MfeI C'AATT_G
Cuts at: 4506 4506
Size: 6907
MluI A'CGCG_T
Cuts at: 3842 6783 3842
Size: 2941 3966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2510 3690 6021 6205 1305
Size: 1205 1180 2331 184 2007
Fragments arranged by size:
2331 2007 1205 1180 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2874 3258 3813 10
Size: 54 1425 343 1042 384 555 3104
Fragments arranged by size:
3104 1425 1042 555 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3141 3157 3885 4032 4865 5224 5383
Size: 648 1975 16 728 147 833 359 159
Cuts at: 5383 518
Size: 2042
Fragments arranged by size:
2042 1975 833 728 648 359 159 147
16
MspA1I CmG'CkG
Cuts at: 1606 2326 3006 3745 4081 4323 5000 6148 6393
Size: 720 680 739 336 242 677 1148 245
Cuts at: 6393 1606
Size: 2120
Fragments arranged by size:
2120 1148 739 720 680 677 336 245
242
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6355
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6907
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6907
NotI GC'GGCC_GC
Cuts at: 3172 4354 3172
Size: 1182 5725
NspI r_CATG'y
Cuts at: 1132 1229 2915 4053 4265 5810 1132
Size: 97 1686 1138 212 1545 2229
Fragments arranged by size:
2229 1686 1545 1138 212 97
PasI CC'CwG_GG
Cuts at: 1348 2733 2774 2844 3018 1348
Size: 1385 41 70 174 5237
Fragments arranged by size:
5237 1385 174 70 41
PciI A'CATG_T
Cuts at: 5806 5806
Size: 6907
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2498 3006 1108
Size: 1390 508 5009
Fragments arranged by size:
5009 1390 508
PfoI T'CCnGG_A
Cuts at: 2589 2589
Size: 6907
PmeI GTTT'AAAC
Cuts at: 4211 4211
Size: 6907
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 4976 5008 6489 6521 3221
Size: 32 1723 32 1481 32 3607
Fragments arranged by size:
3607 1723 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 2428 2711 2848 2950 2113
Size: 315 283 137 102 6070
Fragments arranged by size:
6070 315 283 137 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6907
PsiI TTA'TAA
Cuts at: 4477 4477
Size: 6907
PspOMI G'GGCC_C
Cuts at: 2174 2501 2736 2983 3403 2174
Size: 327 235 247 420 5678
Fragments arranged by size:
5678 420 327 247 235
PstI C_TGCA'G
Cuts at: 830 2550 2790 3375 3594 4259 830
Size: 1720 240 585 219 665 3478
Fragments arranged by size:
3478 1720 665 585 240 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6907
SalI G'TCGA_C
Cuts at: 1936 4249 1936
Size: 2313 4594
SanDI GG'GwC_CC
Cuts at: 2113 2711 2113
Size: 598 6309
SapI GCTCTTCn'nnn_
Cuts at: 3282 3282
Size: 6907
SbfI CC_TGCA'GG
Cuts at: 2550 4259 2550
Size: 1709 5198
ScaI AGT'ACT
Cuts at: 5155 6867 5155
Size: 1712 5195
SfcI C'TryA_G
Cuts at: 826 1045 2546 2786 3371 3590 4255 5390 6071
Size: 219 1501 240 585 219 665 1135 681
Cuts at: 6071 6262 826
Size: 191 1471
Fragments arranged by size:
1501 1471 1135 681 665 585 240 219
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2871 2871
Size: 6907
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6907
SgrAI Cr'CCGG_yG
Cuts at: 1700 3597 3681 3863 4017 1700
Size: 1897 84 182 154 4590
Fragments arranged by size:
4590 1897 182 154 84
SmaI CCC'GGG
Cuts at: 1872 3402 4234 1872
Size: 1530 832 4545
Fragments arranged by size:
4545 1530 832
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3833 5011 5912 6174 820
Size: 197 926 1890 1178 901 262 1553
Fragments arranged by size:
1890 1553 1178 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4698 493
Size: 4205 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6907
SphI G_CATG'C
Cuts at: 1132 2915 4053 4265 1132
Size: 1783 1138 212 3774
Fragments arranged by size:
3774 1783 1138 212
SspI AAT'ATT
Cuts at: 5 52 4831 5
Size: 47 4779 2081
Fragments arranged by size:
4779 2081 47
StuI AGG'CCT
Cuts at: 1517 2011 3734 4070 1517
Size: 494 1723 336 4354
Fragments arranged by size:
4354 1723 494 336
StyI C'CwwG_G
Cuts at: 513 1065 2241 2516 4346 513
Size: 552 1176 275 1830 3074
Fragments arranged by size:
3074 1830 1176 552 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2049 2630 4025 4124 4934 4951 5104
Size: 1255 469 581 1395 99 810 17 153
Cuts at: 5104 5289 5734 325
Size: 185 445 1498
Fragments arranged by size:
1498 1395 1255 810 581 469 445 185
153 99 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5153 6865 96
Size: 274 80 33 51 1443 3176 1712 138
Fragments arranged by size:
3176 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6867 6899 6867
Size: 32 6875
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6907
XbaI T'CTAG_A
Cuts at: 4243 4243
Size: 6907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2696 2696
Size: 6907
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6907
XmaI C'CCGG_G
Cuts at: 1870 3400 4232 1870
Size: 1530 832 4545
Fragments arranged by size:
4545 1530 832
XmnI GAAnn'nnTTC
Cuts at: 4127 5036 4127
Size: 909 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4715 276
Size: 53 83 186 936 3181 2468
Fragments arranged by size:
3181 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SexAI SfoI SrfI SwaI