Restriction map for
pFN21AB6219
(Circular) (Six-base) MAPSORT of: pFN21AB6219.seq Check: 2823 from: 1 to: 6712
pFN21AB6219 6712 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2614 2614
Size: 6712
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2033 4055 1937
Size: 96 2022 4594
Fragments arranged by size:
4594 2022 96
Acc65I G'GTAC_C
Cuts at: 4033 4033
Size: 6712
AclI AA'CG_TT
Cuts at: 1563 4839 5212 1563
Size: 3276 373 3063
Fragments arranged by size:
3276 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 2411 3264 4775 6159 6427 1632
Size: 662 117 853 1511 1384 268 1917
Fragments arranged by size:
1917 1511 1384 853 662 268 117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 5611 6588 1203
Size: 4408 977 1327
Fragments arranged by size:
4408 1327 977
AgeI A'CCGG_T
Cuts at: 5543 5543
Size: 6712
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 2409 2969 1166
Size: 1243 560 4909
Fragments arranged by size:
4909 1243 560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6712
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 6680
AlwNI CAG_nnn'CTG
Cuts at: 2455 2659 4455 6027 2455
Size: 204 1796 1572 3140
Fragments arranged by size:
3140 1796 1572 204
ApaI G_GGCC'C
Cuts at: 2990 3926 3977 2990
Size: 936 51 5725
Fragments arranged by size:
5725 936 51
ApaLI G'TGCA_C
Cuts at: 3380 4769 5925 3380
Size: 1389 1156 4167
Fragments arranged by size:
4167 1389 1156
ApoI r'AATT_y
Cuts at: 1493 4021 4247 4399 6508 1493
Size: 2528 226 152 2109 1697
Fragments arranged by size:
2528 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 3968 4037 1870
Size: 73 118 1907 69 4545
Fragments arranged by size:
4545 1907 118 73 69
BamHI G'GATC_C
Cuts at: 3165 4042 4409 3165
Size: 877 367 5468
Fragments arranged by size:
5468 877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3104 3318 3368 4033 5488
Size: 325 217 627 1317 214 50 665 1455
Cuts at: 5488 618
Size: 1842
Fragments arranged by size:
1842 1455 1317 665 627 325 217 214
50
BanII G_rGCy'C
Cuts at: 1391 2376 2633 2646 2706 2990 3615 3709 3734
Size: 985 257 13 60 284 625 94 25
Cuts at: 3734 3912 3926 3977 4031 1391
Size: 178 14 51 54 4072
Fragments arranged by size:
4072 985 625 284 257 178 94 60
54 51 25 14 13
BbeI G_GCGC'C
Cuts at: 3108 3108
Size: 6712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5741
BbvCI CC'TCA_GC
Cuts at: 2729 3682 2729
Size: 953 5759
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2759 2793 3065 3099 4903 4937 1903
Size: 34 822 34 272 34 1804 34 3678
Fragments arranged by size:
3678 1804 822 272 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4604 5814 4604
Size: 1210 5502
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6712
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5323 136
Size: 107 122 71 631 605 3651 1525
Fragments arranged by size:
3651 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6707 6707
Size: 6712
BlpI GC'TnA_GC
Cuts at: 2885 3650 3997 4129 2885
Size: 765 347 132 5468
Fragments arranged by size:
5468 765 347 132
Bme1580I G_kGCm'C
Cuts at: 1790 2990 3371 3384 3926 3977 4773 5929 1790
Size: 1200 381 13 542 51 796 1156 2573
Fragments arranged by size:
2573 1200 1156 796 542 381 51 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6712
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2720 2986 4443
Size: 416 53 83 399 24 1303 266 1457
Cuts at: 4443 5401 442
Size: 958 1753
Fragments arranged by size:
1753 1457 1303 958 416 399 266 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2390 2604 2720 3083 3585 3643 5372
Size: 1064 87 214 116 363 502 58 1729
Cuts at: 5372 1239
Size: 2579
Fragments arranged by size:
2579 1729 1064 502 363 214 116 87
58
Bpu10I CC'TnA_GC
Cuts at: 2729 3194 3441 3682 2729
Size: 465 247 241 5759
Fragments arranged by size:
5759 465 247 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 3629 4837 5702 6000 2531
Size: 1098 1208 865 298 3243
Fragments arranged by size:
3243 1208 1098 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2087 3035 3766 5375 882
Size: 617 588 948 731 1609 2219
Fragments arranged by size:
2219 1609 948 731 617 588
BsaAI yAC'GTr
Cuts at: 493 1204 4503 493
Size: 711 3299 2702
Fragments arranged by size:
3299 2702 711
BsaBI GATnn'nnATC
Cuts at: 6706 6706
Size: 6712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2433 3105 4519
Size: 53 83 186 936 609 291 672 1414
Cuts at: 4519 4901 5536 275
Size: 382 635 1451
Fragments arranged by size:
1451 1414 936 672 635 609 382 291
186 83 53
BsaWI w'CCGG_w
Cuts at: 3044 5145 5543 5817 5964 6375 3044
Size: 2101 398 274 147 411 3381
Fragments arranged by size:
3381 2101 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6487
Fragments arranged by size:
6487 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 1186
Size: 1249 5463
BseYI C'CCAG_C
Cuts at: 2011 2445 2624 3296 3630 3701 3763 3791 3881
Size: 434 179 672 334 71 62 28 90
Cuts at: 3881 3963 5915 6411 2011
Size: 82 1952 496 2312
Fragments arranged by size:
2312 1952 672 496 434 334 179 90
82 71 62 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3164 1163
Size: 573 1428 4711
Fragments arranged by size:
4711 1428 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4162 4923 5951 1540
Size: 275 186 2161 761 1028 2301
Fragments arranged by size:
2301 2161 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2376 2667 3384 3577 3734 4773 4858 5929
Size: 72 291 717 193 157 1039 85 1071
Cuts at: 5929 2304
Size: 3087
Fragments arranged by size:
3087 1071 1039 717 291 193 157 85
72
BsmI GAATG_Cn'
Cuts at: 4223 4316 4223
Size: 93 6619
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2376 2633 2646 2667 2706 2990
Size: 399 514 72 257 13 21 39 284
Cuts at: 2990 3371 3384 3577 3615 3709 3734 3912 3926
Size: 381 13 193 38 94 25 178 14
Cuts at: 3926 3977 4031 4773 4858 5929 1391
Size: 51 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 399 381 284 257
193 178 94 85 72 54 51 39
38 25 21 14 13 13
BspEI T'CCGG_A
Cuts at: 6375 6375
Size: 6712
BspHI T'CATG_A
Cuts at: 4601 6331 4601
Size: 1730 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 2614 4067 844
Size: 1358 412 1453 3489
Fragments arranged by size:
3489 1453 1358 412
BsrBI CCG'CTC
Cuts at: 1394 1823 2488 4599 1394
Size: 429 665 2111 3507
Fragments arranged by size:
3507 2111 665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5207 5381 65
Size: 1183 3959 174 1396
Fragments arranged by size:
3959 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2079 5356 5543 1700
Size: 110 269 3277 187 2869
Fragments arranged by size:
3277 2869 269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6712
BssHII G'CGCG_C
Cuts at: 1925 2023 1925
Size: 98 6614
BssSI C'ACGA_G
Cuts at: 4772 5784 4772
Size: 1012 5700
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6712
BstBI TT'CG_AA
Cuts at: 5516 5516
Size: 6712
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6712
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6712
BstYI r'GATC_y
Cuts at: 1972 3165 4042 4409 4794 4811 6263 6349 6361
Size: 1193 877 367 385 17 1452 86 12
Cuts at: 6361 6707 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1193 877 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2137 2481 2527 3489 3586
Size: 552 939 33 100 344 46 962 97
Cuts at: 3586 3696 513
Size: 110 3529
Fragments arranged by size:
3529 962 939 552 344 110 100 97
46 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5973
Fragments arranged by size:
5973 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 3423 3929 4228 5022 5042 2660
Size: 763 506 299 794 20 4330
Fragments arranged by size:
4330 794 763 506 299 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 4016 4016
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5719 809
Size: 507 4403 1802
Fragments arranged by size:
4403 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2553 3210 3309
Size: 54 1425 50 275 18 723 657 99
Cuts at: 3309 4159 5048 6677 8
Size: 850 889 1629 43
Fragments arranged by size:
1629 1425 889 850 723 657 275 99
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4159 1537
Size: 275 2347 4090
Fragments arranged by size:
4090 2347 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 3033 3395 3707 4642 1353
Size: 1151 529 362 312 935 3423
Fragments arranged by size:
3423 1151 935 529 362 312
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2812 5299 5673 5819 387
Size: 1064 138 1223 2487 374 146 1280
Fragments arranged by size:
2487 1280 1223 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2374 3732 2374
Size: 1358 5354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2303 2390 2411 2604 2720 3083
Size: 393 662 9 87 21 193 116 363
Cuts at: 3083 3264 3585 3643 4775 5372 6159 6427 1239
Size: 181 321 58 1132 597 787 268 1524
Fragments arranged by size:
1524 1132 787 662 597 393 363 321
268 193 181 116 87 58 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 3197 3669 2159
Size: 1038 472 5202
Fragments arranged by size:
5202 1038 472
EcoO109I rG'GnC_Cy
Cuts at: 2278 2476 2592 2721 2986 2987 3260 3534 3676
Size: 198 116 129 265 1 273 274 142
Cuts at: 3676 3717 3786 3877 3923 3973 3974 2278
Size: 41 69 91 46 50 1 5016
Fragments arranged by size:
5016 274 273 265 198 142 129 116
91 69 50 46 41 1 1
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2441 2441
Size: 6712
FspI TGC'GCA
Cuts at: 2048 2198 2504 2610 5218 2048
Size: 150 306 106 2608 3542
Fragments arranged by size:
3542 2608 306 150 106
HaeII r_GCGC'y
Cuts at: 1678 2012 2151 3108 5859 1678
Size: 334 139 957 2751 2531
Fragments arranged by size:
2751 2531 957 334 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 2373 2405 5353
Size: 32 135 32 578 32 191 32 2948
Cuts at: 5353 5385 5427 5459 1373
Size: 32 42 32 2626
Fragments arranged by size:
2948 2626 578 191 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2034 4056 4302 669
Size: 988 281 96 2022 246 3079
Fragments arranged by size:
3079 2022 988 281 246 96
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6712
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KasI G'GCGC_C
Cuts at: 3104 3104
Size: 6712
KpnI G_GTAC'C
Cuts at: 4037 4037
Size: 6712
MfeI C'AATT_G
Cuts at: 4311 4311
Size: 6712
MluI A'CGCG_T
Cuts at: 6588 6588
Size: 6712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 2877 2976 5826 6010 1305
Size: 1008 564 99 2850 184 2007
Fragments arranged by size:
2850 2007 1008 564 184 99
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3311 10
Size: 54 1425 343 1479 3411
Fragments arranged by size:
3411 1479 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2409 2969 3488 4670 5029 5188 518
Size: 648 1243 560 519 1182 359 159 2042
Fragments arranged by size:
2042 1243 1182 648 560 519 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2139 2368 2529 2624 2650 3100 3215
Size: 507 26 229 161 95 26 450 115
Cuts at: 3215 3307 3630 4128 4805 5953 6198 1606
Size: 92 323 498 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 507 498 450 323 245
229 161 115 95 92 26 26
NaeI GCC'GGC
Cuts at: 2081 2081
Size: 6712
NarI GG'CG_CC
Cuts at: 3105 3105
Size: 6712
NcoI C'CATG_G
Cuts at: 513 1065 2004 3489 3586 3696 513
Size: 552 939 1485 97 110 3529
Fragments arranged by size:
3529 1485 939 552 110 97
NdeI CA'TA_TG
Cuts at: 387 2586 387
Size: 2199 4513
NgoMIV G'CCGG_C
Cuts at: 2079 2079
Size: 6712
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 4159 4159
Size: 6712
NspI r_CATG'y
Cuts at: 1132 1229 2203 4070 5615 1132
Size: 97 974 1867 1545 2229
Fragments arranged by size:
2229 1867 1545 974 97
PasI CC'CwG_GG
Cuts at: 1348 3134 3531 3863 3913 1348
Size: 1786 397 332 50 4147
Fragments arranged by size:
4147 1786 397 332 50
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2743 2845 1108
Size: 1635 102 4975
Fragments arranged by size:
4975 1635 102
PfoI T'CCnGG_A
Cuts at: 2459 3267 3398 2459
Size: 808 131 5773
Fragments arranged by size:
5773 808 131
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3260 3676 3717 3786 3877 3260
Size: 416 41 69 91 6095
Fragments arranged by size:
6095 416 91 69 41
PshAI GACnn'nnGTC
Cuts at: 1613 3246 1613
Size: 1633 5079
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PspOMI G'GGCC_C
Cuts at: 2986 3922 3973 2986
Size: 936 51 5725
Fragments arranged by size:
5725 936 51
PstI C_TGCA'G
Cuts at: 830 2022 2655 3236 3876 4064 830
Size: 1192 633 581 640 188 3478
Fragments arranged by size:
3478 1192 640 633 581 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 2113 2368 2624 2113
Size: 255 256 6201
Fragments arranged by size:
6201 256 255
SacI G_AGCT'C
Cuts at: 2376 3734 2376
Size: 1358 5354
SacII CC_GC'GG
Cuts at: 2140 2530 2140
Size: 390 6322
SalI G'TCGA_C
Cuts at: 1936 2032 4054 1936
Size: 96 2022 4594
Fragments arranged by size:
4594 2022 96
SanDI GG'GwC_CC
Cuts at: 3260 3676 3260
Size: 416 6296
SbfI CC_TGCA'GG
Cuts at: 3876 4064 3876
Size: 188 6524
ScaI AGT'ACT
Cuts at: 4960 6672 4960
Size: 1712 5000
SexAI A'CCwGG_T
Cuts at: 2232 2232
Size: 6712
SfcI C'TryA_G
Cuts at: 826 1045 2018 2651 2794 3232 3872 4060 5195
Size: 219 973 633 143 438 640 188 1135
Cuts at: 5195 5876 6067 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 973 681 640 633 438 219
191 188 143
SfoI GGC'GCC
Cuts at: 3106 3106
Size: 6712
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6712
SmaI CCC'GGG
Cuts at: 1872 2063 3970 4039 1872
Size: 191 1907 69 4545
Fragments arranged by size:
4545 1907 191 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 3608 4816 5717 5979 820
Size: 197 926 603 1062 1208 901 262 1553
Fragments arranged by size:
1553 1208 1062 926 901 603 262 197
SnaBI TAC'GTA
Cuts at: 493 4503 493
Size: 4010 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 2203 4070 1132
Size: 1071 1867 3774
Fragments arranged by size:
3774 1867 1071
SspI AAT'ATT
Cuts at: 5 52 4636 5
Size: 47 4584 2081
Fragments arranged by size:
4584 2081 47
StuI AGG'CCT
Cuts at: 1517 2165 1517
Size: 648 6064
StyI C'CwwG_G
Cuts at: 513 1065 2004 3489 3586 3696 3986 4151 513
Size: 552 939 1485 97 110 290 165 3074
Fragments arranged by size:
3074 1485 939 552 290 165 110 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 2703 4739 4756 4909 5094 5539
Size: 1255 531 592 2036 17 153 185 445
Cuts at: 5539 325
Size: 1498
Fragments arranged by size:
2036 1498 1255 592 531 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 4958 6670
Size: 274 80 33 51 1443 855 2126 1712
Cuts at: 6670 96
Size: 138
Fragments arranged by size:
2126 1712 1443 855 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 6672 6704 3173
Size: 32 3467 32 3181
Fragments arranged by size:
3467 3181 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2455 2858 1316
Size: 1139 403 5170
Fragments arranged by size:
5170 1139 403
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2467 3304 2467
Size: 837 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 2061 3968 4037 1870
Size: 191 1907 69 4545
Fragments arranged by size:
4545 1907 191 69
XmnI GAAnn'nnTTC
Cuts at: 3810 4841 3810
Size: 1031 5681
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SapI SfiI SrfI SwaI