Restriction map for
pFN21AB6215
(Circular) (Six-base) MAPSORT of: pFN21AB6215.seq Check: 6323 from: 1 to: 6712
pFN21AB6215 6712 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4055 1937
Size: 2118 4594
Acc65I G'GTAC_C
Cuts at: 2475 2915 3501 4033 2475
Size: 440 586 532 5154
Fragments arranged by size:
5154 586 532 440
AclI AA'CG_TT
Cuts at: 1563 4839 5212 1563
Size: 3276 373 3063
Fragments arranged by size:
3276 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4775 6159 6427 1632
Size: 3143 1384 268 1917
Fragments arranged by size:
3143 1917 1384 268
AfeI AGC'GCT
Cuts at: 2267 2396 3992 2267
Size: 129 1596 4987
Fragments arranged by size:
4987 1596 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 3970 5611 6588 1203
Size: 2767 1641 977 1327
Fragments arranged by size:
2767 1641 1327 977
AgeI A'CCGG_T
Cuts at: 2288 2648 5543 2288
Size: 360 2895 3457
Fragments arranged by size:
3457 2895 360
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 2291 3747 1166
Size: 1125 1456 4131
Fragments arranged by size:
4131 1456 1125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2762 1184
Size: 1578 5134
AlwNI CAG_nnn'CTG
Cuts at: 3364 3462 3522 4455 6027 3364
Size: 98 60 933 1572 4049
Fragments arranged by size:
4049 1572 933 98 60
ApaI G_GGCC'C
Cuts at: 3059 3059
Size: 6712
ApaLI G'TGCA_C
Cuts at: 4769 5925 4769
Size: 1156 5556
ApoI r'AATT_y
Cuts at: 1493 3611 4021 4247 4399 6508 1493
Size: 2118 410 226 152 2109 1697
Fragments arranged by size:
2118 2109 1697 410 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2879 3072 3758 4037 1870
Size: 73 74 862 193 686 279 4545
Fragments arranged by size:
4545 862 686 279 193 74 73
AvrII C'CTAG_G
Cuts at: 2689 2689
Size: 6712
BamHI G'GATC_C
Cuts at: 2277 3531 4042 4409 2277
Size: 1254 511 367 4580
Fragments arranged by size:
4580 1254 511 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2341 2475 2548 2645 2836
Size: 325 217 627 554 134 73 97 191
Cuts at: 2836 2915 3501 3596 3895 4033 5488 618
Size: 79 586 95 299 138 1455 1842
Fragments arranged by size:
1842 1455 627 586 554 325 299 217
191 138 134 97 95 79 73
BanII G_rGCy'C
Cuts at: 1391 2724 3059 3348 3426 3642 3759 4031 1391
Size: 1333 335 289 78 216 117 272 4072
Fragments arranged by size:
4072 1333 335 289 272 216 117 78
BbeI G_GCGC'C
Cuts at: 2840 2840
Size: 6712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 2530 928
Size: 971 252 379 5110
Fragments arranged by size:
5110 971 379 252
BbvCI CC'TCA_GC
Cuts at: 2196 2196
Size: 6712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2038 2072 4903 4937 1903
Size: 34 101 34 2831 34 3678
Fragments arranged by size:
3678 2831 101 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4604 5814 4604
Size: 1210 5502
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6712
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2032 2058 2834
Size: 107 122 71 631 605 360 26 776
Cuts at: 2834 2971 2980 3001 3040 3229 5323 136
Size: 137 9 21 39 189 2094 1525
Fragments arranged by size:
2094 1525 776 631 605 360 189 137
122 107 71 39 26 21 9
BglII A'GATC_T
Cuts at: 2235 6707 2235
Size: 4472 2240
BlpI GC'TnA_GC
Cuts at: 4129 4129
Size: 6712
Bme1580I G_kGCm'C
Cuts at: 1790 2553 3059 3234 3599 4773 5929 1790
Size: 763 506 175 365 1174 1156 2573
Fragments arranged by size:
2573 1174 1156 763 506 365 175
BmgBI CAC'GTC
Cuts at: 1318 3718 3784 1318
Size: 2400 66 4246
Fragments arranged by size:
4246 2400 66
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2276 3056 4443
Size: 416 53 83 399 24 859 780 1387
Cuts at: 4443 5401 442
Size: 958 1753
Fragments arranged by size:
1753 1387 958 859 780 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2719 2797 2825 3362 5372 1239
Size: 1480 78 28 537 2010 2579
Fragments arranged by size:
2579 2010 1480 537 78 28
Bpu10I CC'TnA_GC
Cuts at: 2196 3560 2196
Size: 1364 5348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4837 5702 6000 4837
Size: 865 298 5549
Fragments arranged by size:
5549 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2300 3486 5375 882
Size: 617 801 1186 1889 2219
Fragments arranged by size:
2219 1889 1186 801 617
BsaAI yAC'GTr
Cuts at: 493 1204 3691 3971 4503 493
Size: 711 2487 280 532 2702
Fragments arranged by size:
2702 2487 711 532 280
BsaBI GATnn'nnATC
Cuts at: 6706 6706
Size: 6712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2386 2837 2963 4519
Size: 53 83 186 936 853 451 126 1556
Cuts at: 4519 4901 5536 275
Size: 382 635 1451
Fragments arranged by size:
1556 1451 936 853 635 451 382 186
126 83 53
BsaWI w'CCGG_w
Cuts at: 2288 2648 5145 5543 5817 5964 6375 2288
Size: 360 2497 398 274 147 411 2625
Fragments arranged by size:
2625 2497 411 398 360 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6487
Fragments arranged by size:
6487 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2084 3491 3872 1186
Size: 859 39 1407 381 4026
Fragments arranged by size:
4026 1407 859 381 39
BseYI C'CCAG_C
Cuts at: 2201 2785 2797 2943 3009 5915 6411 2201
Size: 584 12 146 66 2906 496 2502
Fragments arranged by size:
2906 2502 584 496 146 66 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3771 3869 3965 1163
Size: 573 2035 98 96 3910
Fragments arranged by size:
3910 2035 573 98 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2036 2065 2084 2142 2996 3289
Size: 275 186 35 29 19 58 854 293
Cuts at: 3289 3728 4162 4923 5951 1540
Size: 439 434 761 1028 2301
Fragments arranged by size:
2301 1028 854 761 439 434 293 275
186 58 35 29 19
BsiHKAI G_wGCw'C
Cuts at: 2298 2811 3690 4773 4858 5929 2298
Size: 513 879 1083 85 1071 3081
Fragments arranged by size:
3081 1083 1071 879 513 85
BsmI GAATG_Cn'
Cuts at: 4223 4316 4223
Size: 93 6619
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2553 2724 2811 3059 3234 3348
Size: 399 508 255 171 87 248 175 114
Cuts at: 3348 3426 3599 3642 3690 3759 4031 4773 4858
Size: 78 173 43 48 69 272 742 85
Cuts at: 4858 5929 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 508 399 272 255 248
175 173 171 114 87 85 78 69
48 43
BspEI T'CCGG_A
Cuts at: 6375 6375
Size: 6712
BspHI T'CATG_A
Cuts at: 4601 6331 4601
Size: 1730 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 2385 2754 4067 844
Size: 1355 186 369 1313 3489
Fragments arranged by size:
3489 1355 1313 369 186
BsrBI CCG'CTC
Cuts at: 1394 1823 3040 3085 4599 1394
Size: 429 1217 45 1514 3507
Fragments arranged by size:
3507 1514 1217 429 45
BsrDI GCAATG_nn'
Cuts at: 65 1248 3571 5207 5381 65
Size: 1183 2323 1636 174 1396
Fragments arranged by size:
2323 1636 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2288 2648 2979 2991 5356 5543 1700
Size: 110 478 360 331 12 2365 187 2869
Fragments arranged by size:
2869 2365 478 360 331 187 110 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6712
BssHII G'CGCG_C
Cuts at: 1925 2022 1925
Size: 97 6615
BssSI C'ACGA_G
Cuts at: 4772 5784 4772
Size: 1012 5700
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6712
BstBI TT'CG_AA
Cuts at: 5516 5516
Size: 6712
BstEII G'GTnAC_C
Cuts at: 1184 3308 1184
Size: 2124 4588
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 6712
BstYI r'GATC_y
Cuts at: 1972 2235 2277 3531 4042 4409 4794 4811 6263
Size: 263 42 1254 511 367 385 17 1452
Cuts at: 6263 6349 6361 6707 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1254 511 385 367 346 263
86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2620 2893 2966 3742 513
Size: 552 939 616 273 73 776 3483
Fragments arranged by size:
3483 939 776 616 552 273 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5973
Fragments arranged by size:
5973 683 56
BtsI GCAGTG_nn'
Cuts at: 2781 3899 4228 5022 5042 2781
Size: 1118 329 794 20 4451
Fragments arranged by size:
4451 1118 794 329 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 4016 4016
Size: 6712
DraIII CAC_nnn'GTG
Cuts at: 3488 3488
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2153 5719 809
Size: 507 837 3566 1802
Fragments arranged by size:
3566 1802 837 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 2081 2139
Size: 54 1425 50 275 18 203 48 58
Cuts at: 2139 2972 2993 3135 3408 3725 4159 5048 6677
Size: 833 21 142 273 317 434 889 1629
Cuts at: 6677 8
Size: 43
Fragments arranged by size:
1629 1425 889 833 434 317 275 273
203 142 58 54 50 48 43 21
18
EagI C'GGCC_G
Cuts at: 1537 1812 2033 2081 2139 2993 3725 4159 1537
Size: 275 221 48 58 854 732 434 4090
Fragments arranged by size:
4090 854 732 434 275 221 58 48
EarI CTCTTCn'nnn_
Cuts at: 1353 2298 2768 4642 1353
Size: 945 470 1874 3423
Fragments arranged by size:
3423 1874 945 470
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2218 3779 5299 5673 5819 387
Size: 1064 138 629 1561 1520 374 146 1280
Fragments arranged by size:
1561 1520 1280 1064 629 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2719 2797 2825 3362 4775 5372 6159
Size: 393 1087 78 28 537 1413 597 787
Cuts at: 6159 6427 1239
Size: 268 1524
Fragments arranged by size:
1524 1413 1087 787 597 537 393 268
78 28
EcoO109I rG'GnC_Cy
Cuts at: 2502 3056 3552 3701 2502
Size: 554 496 149 5513
Fragments arranged by size:
5513 554 496 149
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
FseI GG_CCGG'CC
Cuts at: 2983 2983
Size: 6712
FspI TGC'GCA
Cuts at: 2498 5218 2498
Size: 2720 3992
HaeII r_GCGC'y
Cuts at: 1678 2269 2398 2840 3280 3469 3628 3994 5859
Size: 591 129 442 440 189 159 366 1865
Cuts at: 5859 1678
Size: 2531
Fragments arranged by size:
2531 1865 591 442 440 366 189 159
129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5353 5385 5427 5459 1373
Size: 32 135 32 3781 32 42 32 2626
Fragments arranged by size:
3781 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2863 3820 4056 4302 669
Size: 988 281 925 957 236 246 3079
Fragments arranged by size:
3079 988 957 925 281 246 236
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6712
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KasI G'GCGC_C
Cuts at: 2836 2836
Size: 6712
KpnI G_GTAC'C
Cuts at: 2479 2919 3505 4037 2479
Size: 440 586 532 5154
Fragments arranged by size:
5154 586 532 440
MfeI C'AATT_G
Cuts at: 4311 4311
Size: 6712
MluI A'CGCG_T
Cuts at: 6588 6588
Size: 6712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2369 5826 6010 1305
Size: 1064 3457 184 2007
Fragments arranged by size:
3457 2007 1064 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3137 10
Size: 54 1425 343 1305 3585
Fragments arranged by size:
3585 1425 1305 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 3747 4670 5029 5188 518
Size: 648 1125 1456 923 359 159 2042
Fragments arranged by size:
2042 1456 1125 923 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2334 3174 3364 4128 4805 5953 6198
Size: 492 236 840 190 764 677 1148 245
Cuts at: 6198 1606
Size: 2120
Fragments arranged by size:
2120 1148 840 764 677 492 245 236
190
NaeI GCC'GGC
Cuts at: 2981 2981
Size: 6712
NarI GG'CG_CC
Cuts at: 2837 2837
Size: 6712
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5221
Fragments arranged by size:
5221 939 552
NdeI CA'TA_TG
Cuts at: 387 2211 387
Size: 1824 4888
NgoMIV G'CCGG_C
Cuts at: 2979 2979
Size: 6712
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 2081 4159 2081
Size: 2078 4634
NspI r_CATG'y
Cuts at: 1132 1229 4070 5615 1132
Size: 97 2841 1545 2229
Fragments arranged by size:
2841 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3497 1348
Size: 2149 4563
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 2316 2485 2797 3417 3606 1108
Size: 1131 77 169 312 620 189 4214
Fragments arranged by size:
4214 1131 620 312 189 169 77
PfoI T'CCnGG_A
Cuts at: 2541 3204 2541
Size: 663 6049
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PmlI CAC'GTG
Cuts at: 3691 3971 3691
Size: 280 6432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6712
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PspOMI G'GGCC_C
Cuts at: 3055 3055
Size: 6712
PstI C_TGCA'G
Cuts at: 830 4002 4064 830
Size: 3172 62 3478
Fragments arranged by size:
3478 3172 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
SalI G'TCGA_C
Cuts at: 1936 4054 1936
Size: 2118 4594
SbfI CC_TGCA'GG
Cuts at: 4064 4064
Size: 6712
ScaI AGT'ACT
Cuts at: 4960 6672 4960
Size: 1712 5000
SfcI C'TryA_G
Cuts at: 826 1045 3998 4060 5195 5876 6067 826
Size: 219 2953 62 1135 681 191 1471
Fragments arranged by size:
2953 1471 1135 681 219 191 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 2980 3001 2980
Size: 21 6691
SfoI GGC'GCC
Cuts at: 2838 2838
Size: 6712
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 2288 2648 1700
Size: 588 360 5764
Fragments arranged by size:
5764 588 360
SmaI CCC'GGG
Cuts at: 1872 3760 4039 1872
Size: 1888 279 4545
Fragments arranged by size:
4545 1888 279
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4816 5717 5979 820
Size: 197 926 2873 901 262 1553
Fragments arranged by size:
2873 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4503 493
Size: 4010 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 4070 1132
Size: 2938 3774
SspI AAT'ATT
Cuts at: 5 52 4636 5
Size: 47 4584 2081
Fragments arranged by size:
4584 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6712
StyI C'CwwG_G
Cuts at: 513 1065 2004 2128 2310 2689 4151 513
Size: 552 939 124 182 379 1462 3074
Fragments arranged by size:
3074 1462 939 552 379 182 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 4739 4756 4909 5094 5539 325
Size: 1255 1137 2022 17 153 185 445 1498
Fragments arranged by size:
2022 1498 1255 1137 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4958 6670 96
Size: 274 80 33 51 1443 2981 1712 138
Fragments arranged by size:
2981 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6672 6704 6672
Size: 32 6680
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6712
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2830 3376 3705 2830
Size: 546 329 5837
Fragments arranged by size:
5837 546 329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 3758 4037 1870
Size: 1888 279 4545
Fragments arranged by size:
4545 1888 279
XmnI GAAnn'nnTTC
Cuts at: 4841 4841
Size: 6712
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI NruI
NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SrfI SwaI