Restriction map for
pFN21AB6212
(Circular) (Six-base) MAPSORT of: pFN21AB6212.seq Check: 2062 from: 1 to: 6799
pFN21AB6212 6799 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2173 2173
Size: 6799
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3027 4609 278
Size: 53 83 186 936 1491 1582 2468
Fragments arranged by size:
2468 1582 1491 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2247 4142 1937
Size: 310 1895 4594
Fragments arranged by size:
4594 1895 310
Acc65I G'GTAC_C
Cuts at: 2173 2829 4120 2173
Size: 656 1291 4852
Fragments arranged by size:
4852 1291 656
AclI AA'CG_TT
Cuts at: 1563 4926 5299 1563
Size: 3363 373 3063
Fragments arranged by size:
3363 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2765 3737 4862 6246 6514 1632
Size: 1133 972 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1133 1125 972 268
AfeI AGC'GCT
Cuts at: 3823 3823
Size: 6799
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6602
AflIII A'CryG_T
Cuts at: 1203 2202 3471 3519 3873 5698 6675 1203
Size: 999 1269 48 354 1825 977 1327
Fragments arranged by size:
1825 1327 1269 999 977 354 48
AgeI A'CCGG_T
Cuts at: 5630 5630
Size: 6799
AhdI GACnn_n'nnGTC
Cuts at: 5528 5528
Size: 6799
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3011 1184
Size: 1827 4972
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 6767
AlwNI CAG_nnn'CTG
Cuts at: 2536 3289 3460 3628 3715 4542 6114 2536
Size: 753 171 168 87 827 1572 3221
Fragments arranged by size:
3221 1572 827 753 171 168 87
ApaI G_GGCC'C
Cuts at: 2137 4025 2137
Size: 1888 4911
ApaLI G'TGCA_C
Cuts at: 4856 6012 4856
Size: 1156 5643
ApoI r'AATT_y
Cuts at: 1493 2011 4108 4334 4486 6595 1493
Size: 518 2097 226 152 2109 1697
Fragments arranged by size:
2109 2097 1697 518 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5353 160
Size: 872 4321 1606
Fragments arranged by size:
4321 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
AvaI C'yCGr_G
Cuts at: 1870 1943 2288 2858 2963 3188 3501 4124 1870
Size: 73 345 570 105 225 313 623 4545
Fragments arranged by size:
4545 623 570 345 313 225 105 73
BamHI G'GATC_C
Cuts at: 4129 4496 4129
Size: 367 6432
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2173 2345 2702 2829 3194
Size: 325 217 627 386 172 357 127 365
Cuts at: 3194 4120 5575 618
Size: 926 1455 1842
Fragments arranged by size:
1842 1455 926 627 386 365 357 325
217 172 127
BanII G_rGCy'C
Cuts at: 1391 2137 2432 3668 4025 4050 4118 1391
Size: 746 295 1236 357 25 68 4072
Fragments arranged by size:
4072 1236 746 357 295 68 25
BbeI G_GCGC'C
Cuts at: 3198 3198
Size: 6799
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2852 2950 3189 928
Size: 971 953 98 239 4538
Fragments arranged by size:
4538 971 953 239 98
BbvCI CC'TCA_GC
Cuts at: 3317 3317
Size: 6799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4990 5024 1903
Size: 34 50 34 2969 34 3678
Fragments arranged by size:
3678 2969 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2217 4691 5901 2217
Size: 2474 1210 3115
Fragments arranged by size:
3115 2474 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6799
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5410 136
Size: 107 122 71 631 605 3738 1525
Fragments arranged by size:
3738 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6794 6794
Size: 6799
BlpI GC'TnA_GC
Cuts at: 3542 4216 3542
Size: 674 6125
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2350 2705 4025 4860 6016 1790
Size: 347 213 355 1320 835 1156 2573
Fragments arranged by size:
2573 1320 1156 835 355 347 213
BmgBI CAC'GTC
Cuts at: 1318 2956 3313 3474 3522 1318
Size: 1638 357 161 48 4595
Fragments arranged by size:
4595 1638 357 161 48
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2627 3324 4530
Size: 416 53 83 399 24 1210 697 1206
Cuts at: 4530 5488 442
Size: 958 1753
Fragments arranged by size:
1753 1210 1206 958 697 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6799
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 3604 2474
Size: 1130 5669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2425 2513 3074 3130 3650 5459 1239
Size: 1186 88 561 56 520 1809 2579
Fragments arranged by size:
2579 1809 1186 561 520 88 56
Bpu10I CC'TnA_GC
Cuts at: 3317 3317
Size: 6799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4924 5789 6087 4924
Size: 865 298 5636
Fragments arranged by size:
5636 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5462 882
Size: 617 3963 2219
Fragments arranged by size:
3963 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2203 2227 4590 493
Size: 711 999 24 2363 2702
Fragments arranged by size:
2702 2363 999 711 24
BsaBI GATnn'nnATC
Cuts at: 3302 6793 3302
Size: 3491 3308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 3009 3024 3195
Size: 53 83 186 936 708 768 15 171
Cuts at: 3195 3435 3920 4029 4606 4988 5623 275
Size: 240 485 109 577 382 635 1451
Fragments arranged by size:
1451 936 768 708 635 577 485 382
240 186 171 109 83 53 15
BsaWI w'CCGG_w
Cuts at: 2896 3911 5232 5630 5904 6051 6462 2896
Size: 1015 1321 398 274 147 411 3233
Fragments arranged by size:
3233 1321 1015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2229 2259 2630 2660 2855
Size: 30 165 30 826 30 371 30 195
Cuts at: 2855 2885 1178
Size: 30 5092
Fragments arranged by size:
5092 826 371 195 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3133 3274 3623 1186
Size: 1947 141 349 4362
Fragments arranged by size:
4362 1947 349 141
BseYI C'CCAG_C
Cuts at: 2178 2309 2698 2971 3737 6002 6498 2178
Size: 131 389 273 766 2265 496 2479
Fragments arranged by size:
2479 2265 766 496 389 273 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2216 2240 2870 2936 3002 3596
Size: 573 471 9 24 630 66 66 594
Cuts at: 3596 3641 1163
Size: 45 4321
Fragments arranged by size:
4321 630 594 573 471 66 66 45
24 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3927 4249 5010 6038 1540
Size: 275 186 1926 322 761 1028 2301
Fragments arranged by size:
2301 1926 1028 761 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 2622 3668 3894 4860 4945 6016 2622
Size: 1046 226 966 85 1071 3405
Fragments arranged by size:
3405 1071 1046 966 226 85
BsmI GAATG_Cn'
Cuts at: 4310 4403 4310
Size: 93 6706
BsmBI CGTCTCn'nnnn_
Cuts at: 2232 2443 3911 2232
Size: 211 1468 5120
Fragments arranged by size:
5120 1468 211
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2350 2432 2622 2705 3668 3894
Size: 399 347 213 82 190 83 963 226
Cuts at: 3894 4025 4050 4118 4860 4945 6016 1391
Size: 131 25 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 963 742 399 347 226 213
190 131 85 83 82 68 25
BspEI T'CCGG_A
Cuts at: 2896 6462 2896
Size: 3566 3233
BspHI T'CATG_A
Cuts at: 4688 6418 4688
Size: 1730 5069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2161 2173 4154 844
Size: 1317 12 1981 3489
Fragments arranged by size:
3489 1981 1317 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 2511 4686 1394
Size: 429 204 484 2175 3507
Fragments arranged by size:
3507 2175 484 429 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 5294 5468 65
Size: 1183 4046 174 1396
Fragments arranged by size:
4046 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2191 2766 2832 3322 3728 5443 5630
Size: 110 381 575 66 490 406 1715 187
Cuts at: 5630 1700
Size: 2869
Fragments arranged by size:
2869 1715 575 490 406 381 187 110
66
BsrGI T'GTAC_A
Cuts at: 96 3045 96
Size: 2949 3850
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6799
BssSI C'ACGA_G
Cuts at: 3589 4859 5871 3589
Size: 1270 1012 4517
Fragments arranged by size:
4517 1270 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2194 2812 3766 794
Size: 1400 618 954 3827
Fragments arranged by size:
3827 1400 954 618
BstBI TT'CG_AA
Cuts at: 5603 5603
Size: 6799
BstEII G'GTnAC_C
Cuts at: 1184 2353 3703 1184
Size: 1169 1350 4280
Fragments arranged by size:
4280 1350 1169
BstXI CCAn_nnnn'nTGG
Cuts at: 2732 3370 3691 2732
Size: 638 321 5840
Fragments arranged by size:
5840 638 321
BstYI r'GATC_y
Cuts at: 1972 4129 4496 4881 4898 6350 6436 6448 6794
Size: 2157 367 385 17 1452 86 12 346
Cuts at: 6794 1972
Size: 1977
Fragments arranged by size:
2157 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3248 3248
Size: 6799
BtgI C'CryG_G
Cuts at: 513 1065 2043 2358 2412 2464 2907 3792 513
Size: 552 978 315 54 52 443 885 3520
Fragments arranged by size:
3520 978 885 552 443 315 54 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2499 4011 487
Size: 683 56 1273 1512 3275
Fragments arranged by size:
3275 1512 1273 683 56
BtsI GCAGTG_nn'
Cuts at: 2608 3014 3055 4075 4315 5109 5129 2608
Size: 406 41 1020 240 794 20 4278
Fragments arranged by size:
4278 1020 794 406 240 41 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6799
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6764
DraI TTT'AAA
Cuts at: 4103 4103
Size: 6799
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3701 5806 809
Size: 507 2385 2105 1802
Fragments arranged by size:
2385 2105 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2793 2834 2910
Size: 54 1425 50 275 18 963 41 76
Cuts at: 2910 4246 5135 6764 8
Size: 1336 889 1629 43
Fragments arranged by size:
1629 1425 1336 963 889 275 76 54
50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 4246 1537
Size: 275 2434 4090
Fragments arranged by size:
4090 2434 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3171 4729 1353
Size: 1818 1558 3423
Fragments arranged by size:
3423 1818 1558
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3842 5386 5760 5906 387
Size: 1064 138 2253 1544 374 146 1280
Fragments arranged by size:
2253 1544 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3666 3666
Size: 6799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2513 2765 3074 3130 3650 3737
Size: 393 793 88 252 309 56 520 87
Cuts at: 3737 4862 5459 6246 6514 1239
Size: 1125 597 787 268 1524
Fragments arranged by size:
1524 1125 793 787 597 520 393 309
268 252 88 87 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2999 3206 3281 3491 2999
Size: 207 75 210 6307
Fragments arranged by size:
6307 210 207 75
EcoO109I rG'GnC_Cy
Cuts at: 2133 2335 3031 3712 3805 2133
Size: 202 696 681 93 5127
Fragments arranged by size:
5127 696 681 202 93
EcoRI G'AATT_C
Cuts at: 2011 4108 4486 2011
Size: 2097 378 4324
Fragments arranged by size:
4324 2097 378
FspI TGC'GCA
Cuts at: 3818 5305 3818
Size: 1487 5312
HaeII r_GCGC'y
Cuts at: 1678 2978 3198 3290 3469 3825 5946 1678
Size: 1300 220 92 179 356 2121 2531
Fragments arranged by size:
2531 2121 1300 356 220 179 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2229 2261 2855 2887 3941
Size: 32 135 32 657 32 594 32 1054
Cuts at: 3941 3973 5440 5472 5514 5546 1373
Size: 32 1467 32 42 32 2626
Fragments arranged by size:
2626 1467 1054 657 594 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 4143 4389 669
Size: 988 281 1446 759 246 3079
Fragments arranged by size:
3079 1446 988 759 281 246
HindIII A'AGCT_T
Cuts at: 748 3215 748
Size: 2467 4332
HpaI GTT'AAC
Cuts at: 4389 4389
Size: 6799
KasI G'GCGC_C
Cuts at: 3194 3194
Size: 6799
KpnI G_GTAC'C
Cuts at: 2177 2833 4124 2177
Size: 656 1291 4852
Fragments arranged by size:
4852 1291 656
MfeI C'AATT_G
Cuts at: 4398 4398
Size: 6799
MluI A'CGCG_T
Cuts at: 6675 6675
Size: 6799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 5913 6097 1305
Size: 1215 3393 184 2007
Fragments arranged by size:
3393 2007 1215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2795 10
Size: 54 1425 343 963 4014
Fragments arranged by size:
4014 1425 963 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 2730 2795 2912 4757 5116 5275
Size: 648 1245 319 65 117 1845 359 159
Cuts at: 5275 518
Size: 2042
Fragments arranged by size:
2042 1845 1245 648 359 319 159 117
65
MspA1I CmG'CkG
Cuts at: 1606 2381 2558 2868 2880 3040 3625 3687 4215
Size: 775 177 310 12 160 585 62 528
Cuts at: 4215 4892 6040 6285 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 585 528 310 245
177 160 62 12
NaeI GCC'GGC
Cuts at: 3730 3730
Size: 6799
NarI GG'CG_CC
Cuts at: 3195 3195
Size: 6799
NcoI C'CATG_G
Cuts at: 513 1065 2412 2907 3792 513
Size: 552 1347 495 885 3520
Fragments arranged by size:
3520 1347 885 552 495
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6799
NgoMIV G'CCGG_C
Cuts at: 3728 3728
Size: 6799
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6799
NotI GC'GGCC_GC
Cuts at: 4246 4246
Size: 6799
NspI r_CATG'y
Cuts at: 1132 1229 3728 3877 4157 5702 1132
Size: 97 2499 149 280 1545 2229
Fragments arranged by size:
2499 2229 1545 280 149 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6799
PciI A'CATG_T
Cuts at: 3873 5698 3873
Size: 1825 4974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 3289 1108
Size: 1290 891 4618
Fragments arranged by size:
4618 1290 891
PfoI T'CCnGG_A
Cuts at: 3801 3801
Size: 6799
PmeI GTTT'AAAC
Cuts at: 4103 4103
Size: 6799
PmlI CAC'GTG
Cuts at: 2203 2203
Size: 6799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2630 2662 3860 3892 4868 4900 6381 6413 2630
Size: 32 1198 32 976 32 1481 32 3016
Fragments arranged by size:
3016 1481 1198 976 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3712 3805 3712
Size: 93 6706
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6799
PsiI TTA'TAA
Cuts at: 4369 4369
Size: 6799
PspOMI G'GGCC_C
Cuts at: 2133 4021 2133
Size: 1888 4911
PstI C_TGCA'G
Cuts at: 830 2775 3003 3039 3516 3657 4092 4151 830
Size: 1945 228 36 477 141 435 59 3478
Fragments arranged by size:
3478 1945 477 435 228 141 59 36
PvuI CG_AT'CG
Cuts at: 2001 3927 2001
Size: 1926 4873
PvuII CAG'CTG
Cuts at: 3040 3687 3040
Size: 647 6152
SacI G_AGCT'C
Cuts at: 3668 3668
Size: 6799
SalI G'TCGA_C
Cuts at: 1936 4141 1936
Size: 2205 4594
SbfI CC_TGCA'GG
Cuts at: 4092 4151 4092
Size: 59 6740
ScaI AGT'ACT
Cuts at: 5047 6759 5047
Size: 1712 5087
SexAI A'CCwGG_T
Cuts at: 3882 3882
Size: 6799
SfcI C'TryA_G
Cuts at: 826 1045 2771 2999 3035 3069 3512 3653 4088
Size: 219 1726 228 36 34 443 141 435
Cuts at: 4088 4147 5282 5963 6154 826
Size: 59 1135 681 191 1471
Fragments arranged by size:
1726 1471 1135 681 443 435 228 219
191 141 59 36 34
SfoI GGC'GCC
Cuts at: 3196 3196
Size: 6799
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6799
SmaI CCC'GGG
Cuts at: 1872 3503 4126 1872
Size: 1631 623 4545
Fragments arranged by size:
4545 1631 623
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4903 5804 6066 820
Size: 197 926 2960 901 262 1553
Fragments arranged by size:
2960 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4590 493
Size: 4097 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6799
SphI G_CATG'C
Cuts at: 1132 3728 4157 1132
Size: 2596 429 3774
Fragments arranged by size:
3774 2596 429
SrfI GCCC'GGGC
Cuts at: 3503 3503
Size: 6799
SspI AAT'ATT
Cuts at: 5 52 4723 5
Size: 47 4671 2081
Fragments arranged by size:
4671 2081 47
StuI AGG'CCT
Cuts at: 1517 3140 3587 1517
Size: 1623 447 4729
Fragments arranged by size:
4729 1623 447
StyI C'CwwG_G
Cuts at: 513 1065 2412 2907 3207 3426 3747 3792 4238
Size: 552 1347 495 300 219 321 45 446
Cuts at: 4238 513
Size: 3074
Fragments arranged by size:
3074 1347 552 495 446 321 300 219
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3583 3722 4031 4826 4843 4996 5181
Size: 1255 2003 139 309 795 17 153 185
Cuts at: 5181 5626 325
Size: 445 1498
Fragments arranged by size:
2003 1498 1255 795 445 309 185 153
139 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2787 2937 3045
Size: 274 80 33 51 1443 810 150 108
Cuts at: 3045 3870 5045 6757 96
Size: 825 1175 1712 138
Fragments arranged by size:
1712 1443 1175 825 810 274 150 138
108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3151 3183 3333 3365 6759 6791 3151
Size: 32 150 32 3394 32 3159
Fragments arranged by size:
3394 3159 150 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2243 3008 1316
Size: 927 765 5107
Fragments arranged by size:
5107 927 765
XbaI T'CTAG_A
Cuts at: 4135 4135
Size: 6799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2491 2491
Size: 6799
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6799
XmaI C'CCGG_G
Cuts at: 1870 3501 4124 1870
Size: 1631 623 4545
Fragments arranged by size:
4545 1631 623
XmnI GAAnn'nnTTC
Cuts at: 4928 4928
Size: 6799
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3025 4607 276
Size: 53 83 186 936 1491 1582 2468
Fragments arranged by size:
2468 1582 1491 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SacII SanDI SapI SfiI SwaI