Restriction map for
pFN21AB6208
(Circular) (Six-base) MAPSORT of: pFN21AB6208.seq Check: 1509 from: 1 to: 6865
pFN21AB6208 6865 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2133 4675 278
Size: 53 83 186 936 597 2542 2468
Fragments arranged by size:
2542 2468 936 597 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4208 1937
Size: 2271 4594
Acc65I G'GTAC_C
Cuts at: 3060 4186 3060
Size: 1126 5739
AclI AA'CG_TT
Cuts at: 1563 4992 5365 1563
Size: 3429 373 3063
Fragments arranged by size:
3429 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2756 2813 4928 6312 6580 1632
Size: 1124 57 2115 1384 268 1917
Fragments arranged by size:
2115 1917 1384 1124 268 57
AfeI AGC'GCT
Cuts at: 2219 2219
Size: 6865
AflII C'TTAA_G
Cuts at: 820 1017 2714 820
Size: 197 1697 4971
Fragments arranged by size:
4971 1697 197
AflIII A'CryG_T
Cuts at: 1203 2361 5764 6741 1203
Size: 1158 3403 977 1327
Fragments arranged by size:
3403 1327 1158 977
AgeI A'CCGG_T
Cuts at: 3063 5696 3063
Size: 2633 4232
AhdI GACnn_n'nnGTC
Cuts at: 5594 5594
Size: 6865
AleI CACnn'nnGTG
Cuts at: 1166 3950 1166
Size: 2784 4081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2665 1184
Size: 1481 5384
AlwNI CAG_nnn'CTG
Cuts at: 2498 2978 4608 6180 2498
Size: 480 1630 1572 3183
Fragments arranged by size:
3183 1630 1572 480
ApaI G_GGCC'C
Cuts at: 2537 2537
Size: 6865
ApaLI G'TGCA_C
Cuts at: 3125 4922 6078 3125
Size: 1797 1156 3912
Fragments arranged by size:
3912 1797 1156
ApoI r'AATT_y
Cuts at: 1493 2736 4174 4400 4552 6661 1493
Size: 1243 1438 226 152 2109 1697
Fragments arranged by size:
2109 1697 1438 1243 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5419 160
Size: 872 4387 1606
Fragments arranged by size:
4387 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2148 2178 4190 1870
Size: 73 195 10 30 2012 4545
Fragments arranged by size:
4545 2012 195 73 30 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3052 3085 3052
Size: 33 6832
BamHI G'GATC_C
Cuts at: 4195 4562 4195
Size: 367 6498
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2897 3060 3260 3302 3385
Size: 325 217 627 1110 163 200 42 83
Cuts at: 3385 3624 3789 3901 4066 4127 4160 4186 5641
Size: 239 165 112 165 61 33 26 1455
Cuts at: 5641 618
Size: 1842
Fragments arranged by size:
1842 1455 1110 627 325 239 217 200
165 165 163 112 83 61 42 33
26
BanII G_rGCy'C
Cuts at: 1391 2537 2708 2986 3020 3449 3709 3945 4184
Size: 1146 171 278 34 429 260 236 239
Cuts at: 4184 1391
Size: 4072
Fragments arranged by size:
4072 1146 429 278 260 239 236 171
34
BbeI G_GCGC'C
Cuts at: 3628 3793 4131 3628
Size: 165 338 6362
Fragments arranged by size:
6362 338 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5894
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5056 5090 1903
Size: 34 3119 34 3678
Fragments arranged by size:
3678 3119 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4757 5967 4757
Size: 1210 5655
BclI T'GATC_A
Cuts at: 1548 2944 1548
Size: 1396 5469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3024 5476 136
Size: 107 122 71 631 605 1352 2452 1525
Fragments arranged by size:
2452 1525 1352 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6860 6860
Size: 6865
BlpI GC'TnA_GC
Cuts at: 3966 4282 3966
Size: 316 6549
Bme1580I G_kGCm'C
Cuts at: 1790 2537 2900 2954 3129 3307 3500 3665 4163
Size: 747 363 54 175 178 193 165 498
Cuts at: 4163 4926 6082 1790
Size: 763 1156 2573
Fragments arranged by size:
2573 1156 763 747 498 363 193 178
175 165 54
BmgBI CAC'GTC
Cuts at: 1318 2362 2785 1318
Size: 1044 423 5398
Fragments arranged by size:
5398 1044 423
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2435 2662 2934
Size: 416 53 83 399 24 1018 227 272
Cuts at: 2934 3745 4596 5554 442
Size: 811 851 958 1753
Fragments arranged by size:
1753 1018 958 851 811 416 399 272
227 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2174 2308 4021 2174
Size: 134 1713 5018
Fragments arranged by size:
5018 1713 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2438 3000 5525 1239
Size: 1199 562 2525 2579
Fragments arranged by size:
2579 2525 1199 562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4990 5855 6153 4990
Size: 865 298 5702
Fragments arranged by size:
5702 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3264 3681 5528 882
Size: 617 1765 417 1847 2219
Fragments arranged by size:
2219 1847 1765 617 417
BsaAI yAC'GTr
Cuts at: 493 1204 4656 493
Size: 711 3452 2702
Fragments arranged by size:
3452 2702 711
BsaBI GATnn'nnATC
Cuts at: 6859 6859
Size: 6865
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 3625 3790 4128
Size: 53 83 186 936 597 1495 165 338
Cuts at: 4128 4672 5054 5689 275
Size: 544 382 635 1451
Fragments arranged by size:
1495 1451 936 635 597 544 382 338
186 165 83 53
BsaWI w'CCGG_w
Cuts at: 2099 3063 3725 5298 5696 5970 6117 6528 2099
Size: 964 662 1573 398 274 147 411 2436
Fragments arranged by size:
2436 1573 964 662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2354 3431 3461 3684
Size: 30 165 30 921 30 1077 30 223
Cuts at: 3684 3714 1178
Size: 30 4329
Fragments arranged by size:
4329 1077 921 223 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2033 2297 2345 3197 4010 4088 1186
Size: 838 9 264 48 852 813 78 3963
Fragments arranged by size:
3963 852 838 813 264 78 48 9
BseYI C'CCAG_C
Cuts at: 2517 2547 2847 2901 2985 3028 3205 3264 3606
Size: 30 300 54 84 43 177 59 342
Cuts at: 3606 4091 6068 6564 2517
Size: 485 1977 496 2818
Fragments arranged by size:
2818 1977 496 485 342 300 177 84
59 54 43 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3704 1163
Size: 573 1968 4324
Fragments arranged by size:
4324 1968 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4315 5076 6104 1540
Size: 275 186 2314 761 1028 2301
Fragments arranged by size:
2314 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3129 3285 3709 3957 4926 5011 6082 3129
Size: 156 424 248 969 85 1071 3912
Fragments arranged by size:
3912 1071 969 424 248 156 85
BsmI GAATG_Cn'
Cuts at: 2801 3086 4376 4469 2801
Size: 285 1290 93 5197
Fragments arranged by size:
5197 1290 285 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2352 2352
Size: 6865
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2537 2708 2900 2954 2986 3020 3129
Size: 399 747 171 192 54 32 34 109
Cuts at: 3129 3285 3307 3449 3500 3665 3709 3945 3957
Size: 156 22 142 51 165 44 236 12
Cuts at: 3957 4163 4184 4926 5011 6082 1391
Size: 206 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 399 236 206 192
171 165 156 142 109 85 54 51
44 34 32 22 21 12
BspEI T'CCGG_A
Cuts at: 2099 3725 6528 2099
Size: 1626 2803 2436
Fragments arranged by size:
2803 2436 1626
BspHI T'CATG_A
Cuts at: 4754 6484 4754
Size: 1730 5135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3543 4220 844
Size: 2699 677 3489
Fragments arranged by size:
3489 2699 677
BsrBI CCG'CTC
Cuts at: 1394 1823 4752 1394
Size: 429 2929 3507
Fragments arranged by size:
3507 2929 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2765 5360 5534 65
Size: 1183 1517 2595 174 1396
Fragments arranged by size:
2595 1517 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3063 3348 3891 5509 5696 1700
Size: 110 1253 285 543 1618 187 2869
Fragments arranged by size:
2869 1618 1253 543 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 2605 96
Size: 2509 4356
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6865
BssSI C'ACGA_G
Cuts at: 3710 4925 5937 3710
Size: 1215 1012 4638
Fragments arranged by size:
4638 1215 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6865
BstBI TT'CG_AA
Cuts at: 5669 5669
Size: 6865
BstEII G'GTnAC_C
Cuts at: 1184 4030 1184
Size: 2846 4019
BstXI CCAn_nnnn'nTGG
Cuts at: 2759 2909 2993 2759
Size: 150 84 6631
Fragments arranged by size:
6631 150 84
BstYI r'GATC_y
Cuts at: 1972 2656 4195 4562 4947 4964 6416 6502 6514
Size: 684 1539 367 385 17 1452 86 12
Cuts at: 6514 6860 1972
Size: 346 1977
Fragments arranged by size:
1977 1539 1452 684 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3869 3897 3869
Size: 28 6837
BtgI C'CryG_G
Cuts at: 513 1065 2004 2321 2351 3214 513
Size: 552 939 317 30 863 4164
Fragments arranged by size:
4164 939 863 552 317 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3353 487
Size: 683 56 2127 3999
Fragments arranged by size:
3999 2127 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 2872 4381 5175 5195 2586
Size: 286 1509 794 20 4256
Fragments arranged by size:
4256 1509 794 286 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3520 3555 529
Size: 35 2956 35 3839
Fragments arranged by size:
3839 2956 35 35
DraI TTT'AAA
Cuts at: 2166 4169 2166
Size: 2003 4862
DraIII CAC_nnn'GTG
Cuts at: 3159 3289 3385 3590 3159
Size: 130 96 205 6434
Fragments arranged by size:
6434 205 130 96
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5872 809
Size: 507 4556 1802
Fragments arranged by size:
4556 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2919 3435 3508
Size: 54 1425 50 275 18 1089 516 73
Cuts at: 3508 3757 3774 4312 5201 6830 8
Size: 249 17 538 889 1629 43
Fragments arranged by size:
1629 1425 1089 889 538 516 275 249
73 54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 4312 1537
Size: 275 2500 4090
Fragments arranged by size:
4090 2500 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2274 4795 1353
Size: 921 2521 3423
Fragments arranged by size:
3423 2521 921
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 5452 5826 5972 387
Size: 1064 138 441 3422 374 146 1280
Fragments arranged by size:
3422 1280 1064 441 374 146 138
EcoICRI GAG'CTC
Cuts at: 3707 3707
Size: 6865
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2756 2813 3000 4928 5525 6312
Size: 393 806 318 57 187 1928 597 787
Cuts at: 6312 6580 1239
Size: 268 1524
Fragments arranged by size:
1928 1524 806 787 597 393 318 268
187 57
EcoO109I rG'GnC_Cy
Cuts at: 2119 2242 2533 2854 3679 3735 2119
Size: 123 291 321 825 56 5249
Fragments arranged by size:
5249 825 321 291 123 56
EcoRI G'AATT_C
Cuts at: 4174 4552 4174
Size: 378 6487
FspI TGC'GCA
Cuts at: 5371 5371
Size: 6865
HaeII r_GCGC'y
Cuts at: 1678 2048 2221 3628 3718 3793 4131 6012 1678
Size: 370 173 1407 90 75 338 1881 2531
Fragments arranged by size:
2531 1881 1407 370 338 173 90 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 2449 2481 5506
Size: 32 135 32 752 32 93 32 3025
Cuts at: 5506 5538 5580 5612 1373
Size: 32 42 32 2626
Fragments arranged by size:
3025 2626 752 135 93 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4209 4455 669
Size: 988 281 2271 246 3079
Fragments arranged by size:
3079 2271 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6865
HpaI GTT'AAC
Cuts at: 4455 4455
Size: 6865
KasI G'GCGC_C
Cuts at: 3624 3789 4127 3624
Size: 165 338 6362
Fragments arranged by size:
6362 338 165
KpnI G_GTAC'C
Cuts at: 3064 4190 3064
Size: 1126 5739
MfeI C'AATT_G
Cuts at: 4464 4464
Size: 6865
MluI A'CGCG_T
Cuts at: 6741 6741
Size: 6865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5979 6163 1305
Size: 4674 184 2007
Fragments arranged by size:
4674 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2921 3437 3776 10
Size: 54 1425 343 1089 516 339 3099
Fragments arranged by size:
3099 1425 1089 516 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3300 3950 4823 5182 5341 518
Size: 648 2134 650 873 359 159 2042
Fragments arranged by size:
2134 2042 873 650 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2275 2482 2573 3097 3392 3526 3579 3610
Size: 669 207 91 524 295 134 53 31
Cuts at: 3610 3828 4281 4958 6106 6351 1606
Size: 218 453 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 669 524 453 295 245
218 207 134 91 53 31
NaeI GCC'GGC
Cuts at: 3350 3350
Size: 6865
NarI GG'CG_CC
Cuts at: 3625 3790 4128 3625
Size: 165 338 6362
Fragments arranged by size:
6362 338 165
NcoI C'CATG_G
Cuts at: 513 1065 2004 2351 513
Size: 552 939 347 5027
Fragments arranged by size:
5027 939 552 347
NdeI CA'TA_TG
Cuts at: 387 3201 3462 387
Size: 2814 261 3790
Fragments arranged by size:
3790 2814 261
NgoMIV G'CCGG_C
Cuts at: 3348 3348
Size: 6865
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6865
NotI GC'GGCC_GC
Cuts at: 4312 4312
Size: 6865
NspI r_CATG'y
Cuts at: 1132 1229 4223 5768 1132
Size: 97 2994 1545 2229
Fragments arranged by size:
2994 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2627 1348
Size: 1279 5586
PciI A'CATG_T
Cuts at: 5764 5764
Size: 6865
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2978 3205 1108
Size: 1870 227 4768
Fragments arranged by size:
4768 1870 227
PmeI GTTT'AAAC
Cuts at: 4169 4169
Size: 6865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 6447 6479 4934
Size: 32 1481 32 5320
Fragments arranged by size:
5320 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2854 2242
Size: 612 6253
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6865
PsiI TTA'TAA
Cuts at: 4435 4435
Size: 6865
PspOMI G'GGCC_C
Cuts at: 2533 2533
Size: 6865
PstI C_TGCA'G
Cuts at: 830 2253 3911 3963 4217 830
Size: 1423 1658 52 254 3478
Fragments arranged by size:
3478 1658 1423 254 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6865
PvuII CAG'CTG
Cuts at: 2482 2573 3097 3392 3610 2482
Size: 91 524 295 218 5737
Fragments arranged by size:
5737 524 295 218 91
SacI G_AGCT'C
Cuts at: 3709 3709
Size: 6865
SalI G'TCGA_C
Cuts at: 1936 4207 1936
Size: 2271 4594
SanDI GG'GwC_CC
Cuts at: 2242 2242
Size: 6865
SbfI CC_TGCA'GG
Cuts at: 3963 4217 3963
Size: 254 6611
ScaI AGT'ACT
Cuts at: 5113 6825 5113
Size: 1712 5153
SexAI A'CCwGG_T
Cuts at: 3285 3381 3285
Size: 96 6769
SfcI C'TryA_G
Cuts at: 826 1045 2249 2574 3092 3172 3907 3959 4213
Size: 219 1204 325 518 80 735 52 254
Cuts at: 4213 5348 6029 6220 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 735 681 518 325 254
219 191 80 52
SfoI GGC'GCC
Cuts at: 3626 3791 4129 3626
Size: 165 338 6362
Fragments arranged by size:
6362 338 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6865
SmaI CCC'GGG
Cuts at: 1872 2180 4192 1872
Size: 308 2012 4545
Fragments arranged by size:
4545 2012 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2714 4969 5870 6132 820
Size: 197 926 771 2255 901 262 1553
Fragments arranged by size:
2255 1553 926 901 771 262 197
SnaBI TAC'GTA
Cuts at: 493 4656 493
Size: 4163 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6865
SphI G_CATG'C
Cuts at: 1132 4223 1132
Size: 3091 3774
SspI AAT'ATT
Cuts at: 5 52 4789 5
Size: 47 4737 2081
Fragments arranged by size:
4737 2081 47
StuI AGG'CCT
Cuts at: 1517 3254 3338 1517
Size: 1737 84 5044
Fragments arranged by size:
5044 1737 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2191 2208 2295 2351 2742 4304
Size: 552 939 187 17 87 56 391 1562
Cuts at: 4304 513
Size: 3074
Fragments arranged by size:
3074 1562 939 552 391 187 87 56
17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2561 2748 2915 2971 3278 4892 4909
Size: 1255 981 187 167 56 307 1614 17
Cuts at: 4909 5062 5247 5692 325
Size: 153 185 445 1498
Fragments arranged by size:
1614 1498 1255 981 445 307 187 185
167 153 56 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2605 5111 6823
Size: 274 80 33 51 1443 628 2506 1712
Cuts at: 6823 96
Size: 138
Fragments arranged by size:
2506 1712 1443 628 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3684 3716 6825 6857 3684
Size: 32 3109 32 3692
Fragments arranged by size:
3692 3109 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6865
XbaI T'CTAG_A
Cuts at: 4201 4201
Size: 6865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2675 3841 2675
Size: 1166 5699
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6865
XmaI C'CCGG_G
Cuts at: 1870 2178 4190 1870
Size: 308 2012 4545
Fragments arranged by size:
4545 2012 308
XmnI GAAnn'nnTTC
Cuts at: 4994 4994
Size: 6865
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2131 4673 276
Size: 53 83 186 936 597 2542 2468
Fragments arranged by size:
2542 2468 936 597 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SapI
SfiI SrfI SwaI