Restriction map for
pFN21AB6207
(Circular) (Six-base) MAPSORT of: pFN21AB6207.seq Check: 3655 from: 1 to: 6700
pFN21AB6207 6700 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4510 278
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4043 1937
Size: 2106 4594
Acc65I G'GTAC_C
Cuts at: 4021 4021
Size: 6700
AclI AA'CG_TT
Cuts at: 1563 4827 5200 1563
Size: 3264 373 3063
Fragments arranged by size:
3264 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 2528 3112 3448 3503 3671 3700 3868
Size: 821 75 584 336 55 168 29 168
Cuts at: 3868 4763 6147 6415 1632
Size: 895 1384 268 1917
Fragments arranged by size:
1917 1384 895 821 584 336 268 168
168 75 55 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6503
AflIII A'CryG_T
Cuts at: 1203 3826 5599 6576 1203
Size: 2623 1773 977 1327
Fragments arranged by size:
2623 1773 1327 977
AgeI A'CCGG_T
Cuts at: 5531 5531
Size: 6700
AhdI GACnn_n'nnGTC
Cuts at: 3621 5429 3621
Size: 1808 4892
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3196 1184
Size: 2012 4688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2964 2996 2964
Size: 32 6668
AlwNI CAG_nnn'CTG
Cuts at: 4443 6015 4443
Size: 1572 5128
ApaI G_GGCC'C
Cuts at: 3990 3990
Size: 6700
ApaLI G'TGCA_C
Cuts at: 3572 4757 5913 3572
Size: 1185 1156 4359
Fragments arranged by size:
4359 1185 1156
ApoI r'AATT_y
Cuts at: 1493 2994 3037 4009 4235 4387 6496 1493
Size: 1501 43 972 226 152 2109 1697
Fragments arranged by size:
2109 1697 1501 972 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 5254 160
Size: 872 4222 1606
Fragments arranged by size:
4222 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 3071 4025 1870
Size: 73 433 695 954 4545
Fragments arranged by size:
4545 954 695 433 73
BamHI G'GATC_C
Cuts at: 4030 4397 4030
Size: 367 6333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2341 4021 5476 618
Size: 325 217 627 554 1680 1455 1842
Fragments arranged by size:
1842 1680 1455 627 554 325 217
BanII G_rGCy'C
Cuts at: 1391 3726 3990 4019 1391
Size: 2335 264 29 4072
Fragments arranged by size:
4072 2335 264 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3167 3491 3801 928
Size: 971 1268 324 310 3827
Fragments arranged by size:
3827 1268 971 324 310
BbvCI CC'TCA_GC
Cuts at: 2913 2913
Size: 6700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4891 4925 1903
Size: 34 2954 34 3678
Fragments arranged by size:
3678 2954 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3576 4592 5802 3576
Size: 1016 1210 4474
Fragments arranged by size:
4474 1210 1016
BclI T'GATC_A
Cuts at: 1548 2734 1548
Size: 1186 5514
BfrBI ATG'CAT
Cuts at: 3104 3776 3104
Size: 672 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2729 5311 136
Size: 107 122 71 631 605 1057 2582 1525
Fragments arranged by size:
2582 1525 1057 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6695 6695
Size: 6700
BlpI GC'TnA_GC
Cuts at: 4117 4117
Size: 6700
Bme1580I G_kGCm'C
Cuts at: 1790 2346 3058 3072 3576 3990 4761 5917 1790
Size: 556 712 14 504 414 771 1156 2573
Fragments arranged by size:
2573 1156 771 712 556 504 414 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2145 2489 4431
Size: 416 53 83 399 24 728 344 1942
Cuts at: 4431 5389 442
Size: 958 1753
Fragments arranged by size:
1942 1753 958 728 416 399 344 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6700
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2225 2945 2225
Size: 720 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2278 2381 2723 2940 3024 3444 3696
Size: 871 168 103 342 217 84 420 252
Cuts at: 3696 5360 1239
Size: 1664 2579
Fragments arranged by size:
2579 1664 871 420 342 252 217 168
103 84
Bpu10I CC'TnA_GC
Cuts at: 2913 2913
Size: 6700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2969 3652 4825 5690 5988 2969
Size: 683 1173 865 298 3681
Fragments arranged by size:
3681 1173 865 683 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2418 3844 3929 5363 882
Size: 617 919 1426 85 1434 2219
Fragments arranged by size:
2219 1434 1426 919 617 85
BsaAI yAC'GTr
Cuts at: 493 1204 2528 3409 4491 493
Size: 711 1324 881 1082 2702
Fragments arranged by size:
2702 1324 1082 881 711
BsaBI GATnn'nnATC
Cuts at: 6694 6694
Size: 6700
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2805 4507 4889 5524
Size: 53 83 186 936 1272 1702 382 635
Cuts at: 5524 275
Size: 1451
Fragments arranged by size:
1702 1451 1272 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3254 3549 5133 5531 5805 5952 6363 3254
Size: 295 1584 398 274 147 411 3591
Fragments arranged by size:
3591 1584 411 398 295 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3587 3617 1178
Size: 30 165 30 2184 30 4261
Fragments arranged by size:
4261 2184 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 2692 2793 3886 1186
Size: 906 600 101 1093 4000
Fragments arranged by size:
4000 1093 906 600 101
BseYI C'CCAG_C
Cuts at: 2163 2167 5903 6399 2163
Size: 4 3736 496 2464
Fragments arranged by size:
3736 2464 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2131 2508 2844 1163
Size: 573 395 377 336 5019
Fragments arranged by size:
5019 573 395 377 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4150 4911 5939 1540
Size: 275 186 2149 761 1028 2301
Fragments arranged by size:
2301 2149 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3560 3576 4761 4846 5917 3560
Size: 16 1185 85 1071 4343
Fragments arranged by size:
4343 1185 1071 85 16
BsmI GAATG_Cn'
Cuts at: 3446 3530 3791 4211 4304 3446
Size: 84 261 420 93 5842
Fragments arranged by size:
5842 420 261 93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 3058 3072 3560 3576 3726 3990
Size: 399 556 712 14 488 16 150 264
Cuts at: 3990 4019 4761 4846 5917 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 556 488 399 264
150 85 29 16 14
BspEI T'CCGG_A
Cuts at: 3549 6363 3549
Size: 2814 3886
BspHI T'CATG_A
Cuts at: 4589 6319 4589
Size: 1730 4970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4055 844
Size: 3211 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4587 1394
Size: 429 2764 3507
Fragments arranged by size:
3507 2764 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2057 5195 5369 65
Size: 1183 809 3138 174 1396
Fragments arranged by size:
3138 1396 1183 809 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 5344 5531 1700
Size: 110 575 2959 187 2869
Fragments arranged by size:
2959 2869 575 187 110
BsrGI T'GTAC_A
Cuts at: 96 3823 3907 96
Size: 3727 84 2889
Fragments arranged by size:
3727 2889 84
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6700
BssSI C'ACGA_G
Cuts at: 3298 4760 5772 3298
Size: 1462 1012 4226
Fragments arranged by size:
4226 1462 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2341 794
Size: 1547 5153
BstBI TT'CG_AA
Cuts at: 5504 5504
Size: 6700
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6700
BstXI CCAn_nnnn'nTGG
Cuts at: 2652 3704 2652
Size: 1052 5648
BstYI r'GATC_y
Cuts at: 1972 2272 2880 4030 4397 4782 4799 6251 6337
Size: 300 608 1150 367 385 17 1452 86
Cuts at: 6337 6349 6695 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1150 608 385 367 346 300
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2327 3398 3734 2327
Size: 1071 336 5293
Fragments arranged by size:
5293 1071 336
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2495 513
Size: 552 939 30 461 4718
Fragments arranged by size:
4718 939 552 461 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5961
Fragments arranged by size:
5961 683 56
BtsI GCAGTG_nn'
Cuts at: 2792 4216 5010 5030 2792
Size: 1424 794 20 4462
Fragments arranged by size:
4462 1424 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6700
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2206 2241 3517 3552 529
Size: 35 1642 35 1276 35 3677
Fragments arranged by size:
3677 1642 1276 35 35 35
DraI TTT'AAA
Cuts at: 4004 4004
Size: 6700
DraIII CAC_nnn'GTG
Cuts at: 3842 3842
Size: 6700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2804 5707 809
Size: 507 1488 2903 1802
Fragments arranged by size:
2903 1802 1488 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2387 2502
Size: 54 1425 50 275 18 201 356 115
Cuts at: 2502 4147 5036 6665 8
Size: 1645 889 1629 43
Fragments arranged by size:
1645 1629 1425 889 356 275 201 115
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4147 1537
Size: 275 2335 4090
Fragments arranged by size:
4090 2335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2247 2349 3481 3647 3925 4630 1353
Size: 894 102 1132 166 278 705 3423
Fragments arranged by size:
3423 1132 894 705 278 166 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5287 5661 5807 387
Size: 1064 138 3698 374 146 1280
Fragments arranged by size:
3698 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2278 2381 2453 2528 2723 2940
Size: 393 478 168 103 72 75 195 217
Cuts at: 2940 3024 3112 3444 3448 3503 3671 3696 3700
Size: 84 88 332 4 55 168 25 4
Cuts at: 3700 3868 4763 5360 6147 6415 1239
Size: 168 895 597 787 268 1524
Fragments arranged by size:
1524 895 787 597 478 393 332 268
217 195 168 168 168 103 88 84
75 72 55 25 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3298 3298
Size: 6700
EcoO109I rG'GnC_Cy
Cuts at: 2115 3987 2115
Size: 1872 4828
EcoRI G'AATT_C
Cuts at: 2994 4009 4387 2994
Size: 1015 378 5307
Fragments arranged by size:
5307 1015 378
FspI TGC'GCA
Cuts at: 5206 5206
Size: 6700
HaeII r_GCGC'y
Cuts at: 1678 5847 1678
Size: 4169 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2870 2902 3544 3547 3576
Size: 32 135 32 1298 32 642 3 29
Cuts at: 3576 3579 3607 3639 3713 3745 3841 3873 5341
Size: 3 28 32 74 32 96 32 1468
Cuts at: 5341 5373 5415 5447 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1468 1298 642 135 96 74 42
32 32 32 32 32 32 32 32
29 28 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2840 4044 4290 669
Size: 988 281 902 1204 246 3079
Fragments arranged by size:
3079 1204 988 902 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6700
HpaI GTT'AAC
Cuts at: 4290 4290
Size: 6700
KpnI G_GTAC'C
Cuts at: 4025 4025
Size: 6700
MfeI C'AATT_G
Cuts at: 4299 4299
Size: 6700
MluI A'CGCG_T
Cuts at: 6576 6576
Size: 6700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5814 5998 1305
Size: 4509 184 2007
Fragments arranged by size:
4509 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2504 10
Size: 54 1425 343 672 4206
Fragments arranged by size:
4206 1425 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2894 3271 3523 4658 5017 5176
Size: 648 1140 588 377 252 1135 359 159
Cuts at: 5176 518
Size: 2042
Fragments arranged by size:
2042 1140 1135 648 588 377 359 252
159
MspA1I CmG'CkG
Cuts at: 1606 2167 2338 4116 4793 5941 6186 1606
Size: 561 171 1778 677 1148 245 2120
Fragments arranged by size:
2120 1778 1148 677 561 245 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 2495 513
Size: 552 939 491 4718
Fragments arranged by size:
4718 939 552 491
NdeI CA'TA_TG
Cuts at: 387 3520 387
Size: 3133 3567
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6700
NotI GC'GGCC_GC
Cuts at: 4147 4147
Size: 6700
NsiI A_TGCA'T
Cuts at: 3106 3778 3106
Size: 672 6028
NspI r_CATG'y
Cuts at: 1132 1229 2305 3108 3664 3746 3780 3830 4058
Size: 97 1076 803 556 82 34 50 228
Cuts at: 4058 5603 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1076 803 556 228 97 82
50 34
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6700
PciI A'CATG_T
Cuts at: 3826 5599 3826
Size: 1773 4927
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3983 1108
Size: 2875 3825
PfoI T'CCnGG_A
Cuts at: 2046 2397 2046
Size: 351 6349
PmeI GTTT'AAAC
Cuts at: 4004 4004
Size: 6700
PmlI CAC'GTG
Cuts at: 2528 3409 2528
Size: 881 5819
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2740 4769 4801 6282 6314 2708
Size: 32 2029 32 1481 32 3094
Fragments arranged by size:
3094 2029 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2115 2115
Size: 6700
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6700
PsiI TTA'TAA
Cuts at: 4270 4270
Size: 6700
PspOMI G'GGCC_C
Cuts at: 3986 3986
Size: 6700
PstI C_TGCA'G
Cuts at: 830 3175 4052 830
Size: 2345 877 3478
Fragments arranged by size:
3478 2345 877
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6700
PvuII CAG'CTG
Cuts at: 2167 2338 2167
Size: 171 6529
SalI G'TCGA_C
Cuts at: 1936 4042 1936
Size: 2106 4594
SbfI CC_TGCA'GG
Cuts at: 4052 4052
Size: 6700
ScaI AGT'ACT
Cuts at: 4948 6660 4948
Size: 1712 4988
SfcI C'TryA_G
Cuts at: 826 1045 2710 3171 4048 5183 5864 6055 826
Size: 219 1665 461 877 1135 681 191 1471
Fragments arranged by size:
1665 1471 1135 877 681 461 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6700
SmaI CCC'GGG
Cuts at: 1872 4027 1872
Size: 2155 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2376 2948 3631 4804 5705 5967
Size: 197 926 433 572 683 1173 901 262
Cuts at: 5967 820
Size: 1553
Fragments arranged by size:
1553 1173 926 901 683 572 433 262
197
SnaBI TAC'GTA
Cuts at: 493 4491 493
Size: 3998 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6700
SphI G_CATG'C
Cuts at: 1132 3780 4058 1132
Size: 2648 278 3774
Fragments arranged by size:
3774 2648 278
SspI AAT'ATT
Cuts at: 5 52 3218 4624 5
Size: 47 3166 1406 2081
Fragments arranged by size:
3166 2081 1406 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6700
StyI C'CwwG_G
Cuts at: 513 1065 2004 2408 2495 4139 513
Size: 552 939 404 87 1644 3074
Fragments arranged by size:
3074 1644 939 552 404 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2488 2644 4727 4744 4897 5082 5527
Size: 1255 908 156 2083 17 153 185 445
Cuts at: 5527 325
Size: 1498
Fragments arranged by size:
2083 1498 1255 908 445 185 156 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3823 3907 4946
Size: 274 80 33 51 1443 1846 84 1039
Cuts at: 4946 6658 96
Size: 1712 138
Fragments arranged by size:
1846 1712 1443 1039 274 138 84 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2197 2229 3742 3774 6660 6692 2197
Size: 32 1513 32 2886 32 2205
Fragments arranged by size:
2886 2205 1513 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6700
XbaI T'CTAG_A
Cuts at: 4036 4036
Size: 6700
XhoI C'TCGA_G
Cuts at: 1943 2376 1943
Size: 433 6267
XmaI C'CCGG_G
Cuts at: 1870 4025 1870
Size: 2155 4545
XmnI GAAnn'nnTTC
Cuts at: 3293 3377 3461 3881 4829 3293
Size: 84 84 420 948 5164
Fragments arranged by size:
5164 948 420 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4508 276
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI