Restriction map for
pFN21AB6206
(Circular) (Six-base) MAPSORT of: pFN21AB6206.seq Check: 5898 from: 1 to: 6850
pFN21AB6206 6850 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4660 278
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4193 1937
Size: 2256 4594
Acc65I G'GTAC_C
Cuts at: 4171 4171
Size: 6850
AclI AA'CG_TT
Cuts at: 1563 2703 4977 5350 1563
Size: 1140 2274 373 3063
Fragments arranged by size:
3063 2274 1140 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2824 2965 3322 3406 3658 3742 4913 6297
Size: 1192 141 357 84 252 84 1171 1384
Cuts at: 6297 6565 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1192 1171 357 268 252 141
84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6653
AflIII A'CryG_T
Cuts at: 1203 5749 6726 1203
Size: 4546 977 1327
Fragments arranged by size:
4546 1327 977
AgeI A'CCGG_T
Cuts at: 5681 5681
Size: 6850
AhdI GACnn_n'nnGTC
Cuts at: 5579 5579
Size: 6850
AleI CACnn'nnGTG
Cuts at: 1166 3969 1166
Size: 2803 4047
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2062 1184
Size: 878 5972
AlwNI CAG_nnn'CTG
Cuts at: 4593 6165 4593
Size: 1572 5278
ApaLI G'TGCA_C
Cuts at: 3400 4907 6063 3400
Size: 1507 1156 4187
Fragments arranged by size:
4187 1507 1156
ApoI r'AATT_y
Cuts at: 1493 2281 2593 3288 3372 3624 3708 3876 4044
Size: 788 312 695 84 252 84 168 168
Cuts at: 4044 4159 4385 4537 6646 1493
Size: 115 226 152 2109 1697
Fragments arranged by size:
2109 1697 788 695 312 252 226 168
168 152 115 84 84
AseI AT'TA_AT
Cuts at: 160 1032 2607 5404 160
Size: 872 1575 2797 1606
Fragments arranged by size:
2797 1606 1575 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
AvaI C'yCGr_G
Cuts at: 1870 1943 2244 4175 1870
Size: 73 301 1931 4545
Fragments arranged by size:
4545 1931 301 73
BamHI G'GATC_C
Cuts at: 4180 4547 4180
Size: 367 6483
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4171 5626 618
Size: 325 217 627 2384 1455 1842
Fragments arranged by size:
2384 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3938 4022 4169 1391
Size: 2547 84 147 4072
Fragments arranged by size:
4072 2547 147 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5041 5075 1903
Size: 34 3104 34 3678
Fragments arranged by size:
3678 3104 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4742 5952 4742
Size: 1210 5640
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6850
BfrBI ATG'CAT
Cuts at: 3952 3952
Size: 6850
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5461 136
Size: 107 122 71 631 605 3789 1525
Fragments arranged by size:
3789 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6845 6845
Size: 6850
BlpI GC'TnA_GC
Cuts at: 4267 4267
Size: 6850
Bme1580I G_kGCm'C
Cuts at: 1790 3404 4911 6067 1790
Size: 1614 1507 1156 2573
Fragments arranged by size:
2573 1614 1507 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6850
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3894 4581 5539
Size: 416 53 83 399 24 2477 687 958
Cuts at: 5539 442
Size: 1753
Fragments arranged by size:
2477 1753 958 687 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6850
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 6850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2222 2988 3150 3234 3318 3402 3486
Size: 917 66 766 162 84 84 84 84
Cuts at: 3486 3570 3654 3738 3822 4074 4158 5510 1239
Size: 84 84 84 84 252 84 1352 2579
Fragments arranged by size:
2579 1352 917 766 252 162 84 84
84 84 84 84 84 84 84 66
Bpu10I CC'TnA_GC
Cuts at: 2559 2559
Size: 6850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 2443 2707 4975 5840 6138 2285
Size: 158 264 2268 865 298 2997
Fragments arranged by size:
2997 2268 865 298 264 158
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2132 2966 5513 882
Size: 617 633 834 2547 2219
Fragments arranged by size:
2547 2219 834 633 617
BsaAI yAC'GTr
Cuts at: 493 1204 2323 4641 493
Size: 711 1119 2318 2702
Fragments arranged by size:
2702 2318 1119 711
BsaBI GATnn'nnATC
Cuts at: 6844 6844
Size: 6850
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4657 5039 5674 275
Size: 53 83 186 936 3124 382 635 1451
Fragments arranged by size:
3124 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2344 5283 5681 5955 6102 6513 2344
Size: 2939 398 274 147 411 2681
Fragments arranged by size:
2939 2681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 3881 3911 1178
Size: 30 165 30 858 30 1590 30 4117
Fragments arranged by size:
4117 1590 858 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2282 2363 1186
Size: 910 186 81 5673
Fragments arranged by size:
5673 910 186 81
BseYI C'CCAG_C
Cuts at: 6053 6549 6053
Size: 496 6354
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2242 2932 1163
Size: 573 506 690 5081
Fragments arranged by size:
5081 690 573 506
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4300 5061 6089 1540
Size: 275 186 2299 761 1028 2301
Fragments arranged by size:
2301 2299 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3404 3938 4022 4911 4996 6067 3404
Size: 534 84 889 85 1071 4187
Fragments arranged by size:
4187 1071 889 534 85 84
BsiWI C'GTAC_G
Cuts at: 3729 3729
Size: 6850
BsmI GAATG_Cn'
Cuts at: 2069 3329 4361 4454 2069
Size: 1260 1032 93 4465
Fragments arranged by size:
4465 1260 1032 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3404 3938 4022 4169 4911 4996 6067
Size: 399 1614 534 84 147 742 85 1071
Cuts at: 6067 1391
Size: 2174
Fragments arranged by size:
2174 1614 1071 742 534 399 147 85
84
BspEI T'CCGG_A
Cuts at: 6513 6513
Size: 6850
BspHI T'CATG_A
Cuts at: 2611 4739 6469 2611
Size: 2128 1730 2992
Fragments arranged by size:
2992 2128 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4205 844
Size: 3361 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4737 1394
Size: 429 2914 3507
Fragments arranged by size:
3507 2914 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5345 5519 65
Size: 1183 4097 174 1396
Fragments arranged by size:
4097 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5494 5681 1700
Size: 110 3684 187 2869
Fragments arranged by size:
3684 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2331 2944 3361 3529 96
Size: 2235 613 417 168 3417
Fragments arranged by size:
3417 2235 613 417 168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6850
BssSI C'ACGA_G
Cuts at: 4910 5922 4910
Size: 1012 5838
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3912 794
Size: 3118 3732
BstBI TT'CG_AA
Cuts at: 3144 5654 3144
Size: 2510 4340
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6850
BstYI r'GATC_y
Cuts at: 1972 3263 4180 4547 4932 4949 6401 6487 6499
Size: 1291 917 367 385 17 1452 86 12
Cuts at: 6499 6845 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1291 917 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3012 513
Size: 552 939 1008 4351
Fragments arranged by size:
4351 1008 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6111
Fragments arranged by size:
6111 683 56
BtsI GCAGTG_nn'
Cuts at: 4366 5160 5180 4366
Size: 794 20 6036
Fragments arranged by size:
6036 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2343 2378 3559 3594 529
Size: 35 1779 35 1181 35 3785
Fragments arranged by size:
3785 1779 1181 35 35 35
DraI TTT'AAA
Cuts at: 4154 4154
Size: 6850
DraIII CAC_nnn'GTG
Cuts at: 2323 2851 2323
Size: 528 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5857 809
Size: 507 4541 1802
Fragments arranged by size:
4541 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4297 5186 6815
Size: 54 1425 50 275 18 2467 889 1629
Cuts at: 6815 8
Size: 43
Fragments arranged by size:
2467 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4297 1537
Size: 275 2485 4090
Fragments arranged by size:
4090 2485 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2205 2843 4780 1353
Size: 852 638 1937 3423
Fragments arranged by size:
3423 1937 852 638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 5437 5811 5957 387
Size: 1064 138 2552 1296 374 146 1280
Fragments arranged by size:
2552 1296 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3936 4020 3936
Size: 84 6766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2222 2824 2965 2988 3150 3234
Size: 393 524 66 602 141 23 162 84
Cuts at: 3234 3318 3322 3402 3406 3486 3570 3654 3658
Size: 84 4 80 4 80 84 84 4
Cuts at: 3658 3738 3742 3822 4074 4158 4913 5510 6297
Size: 80 4 80 252 84 755 597 787
Cuts at: 6297 6565 1239
Size: 268 1524
Fragments arranged by size:
1524 787 755 602 597 524 393 268
252 162 141 84 84 84 84 84
80 80 80 80 66 23 4 4
4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3928 3928
Size: 6850
EcoO109I rG'GnC_Cy
Cuts at: 2215 2239 2215
Size: 24 6826
EcoRI G'AATT_C
Cuts at: 2281 3288 3372 3624 3708 4044 4159 4537 2281
Size: 1007 84 252 84 336 115 378 4594
Fragments arranged by size:
4594 1007 378 336 252 115 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2279 2279
Size: 6850
FspI TGC'GCA
Cuts at: 5356 5356
Size: 6850
HaeII r_GCGC'y
Cuts at: 1678 5997 1678
Size: 4319 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 3047 3079 5491
Size: 32 135 32 605 32 838 32 2412
Cuts at: 5491 5523 5565 5597 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2412 838 605 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4194 4440 669
Size: 988 281 2256 246 3079
Fragments arranged by size:
3079 2256 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6850
HpaI GTT'AAC
Cuts at: 4440 4440
Size: 6850
KpnI G_GTAC'C
Cuts at: 4175 4175
Size: 6850
MfeI C'AATT_G
Cuts at: 4449 4449
Size: 6850
MluI A'CGCG_T
Cuts at: 6726 6726
Size: 6850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5964 6148 1305
Size: 4659 184 2007
Fragments arranged by size:
4659 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5028
Fragments arranged by size:
5028 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2044 2655 3067 3969 4808 5167 5326
Size: 648 878 611 412 902 839 359 159
Cuts at: 5326 518
Size: 2042
Fragments arranged by size:
2042 902 878 839 648 611 412 359
159
MspA1I CmG'CkG
Cuts at: 1606 2095 2200 3096 4266 4943 6091 6336 1606
Size: 489 105 896 1170 677 1148 245 2120
Fragments arranged by size:
2120 1170 1148 896 677 489 245 105
NcoI C'CATG_G
Cuts at: 513 1065 2004 3012 513
Size: 552 939 1008 4351
Fragments arranged by size:
4351 1008 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6850
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6850
NotI GC'GGCC_GC
Cuts at: 4297 4297
Size: 6850
NsiI A_TGCA'T
Cuts at: 3954 3954
Size: 6850
NspI r_CATG'y
Cuts at: 1132 1229 4208 5753 1132
Size: 97 2979 1545 2229
Fragments arranged by size:
2979 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2161 1348
Size: 813 6037
PciI A'CATG_T
Cuts at: 5749 5749
Size: 6850
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 5635
PmeI GTTT'AAAC
Cuts at: 4154 4154
Size: 6850
PmlI CAC'GTG
Cuts at: 2323 2323
Size: 6850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4919 4951 6432 6464 4919
Size: 32 1481 32 5305
Fragments arranged by size:
5305 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 2215
Size: 6850
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6850
PsiI TTA'TAA
Cuts at: 4420 4420
Size: 6850
PstI C_TGCA'G
Cuts at: 830 4202 830
Size: 3372 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6850
PvuII CAG'CTG
Cuts at: 2095 2200 3096 2095
Size: 105 896 5849
Fragments arranged by size:
5849 896 105
SacI G_AGCT'C
Cuts at: 3938 4022 3938
Size: 84 6766
SalI G'TCGA_C
Cuts at: 1936 4192 1936
Size: 2256 4594
SbfI CC_TGCA'GG
Cuts at: 4202 4202
Size: 6850
ScaI AGT'ACT
Cuts at: 5098 6810 5098
Size: 1712 5138
SfcI C'TryA_G
Cuts at: 826 1045 3051 4198 5333 6014 6205 826
Size: 219 2006 1147 1135 681 191 1471
Fragments arranged by size:
2006 1471 1147 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6850
SmaI CCC'GGG
Cuts at: 1872 4177 1872
Size: 2305 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 2422 2686 4954 5855 6117
Size: 197 926 321 158 264 2268 901 262
Cuts at: 6117 820
Size: 1553
Fragments arranged by size:
2268 1553 926 901 321 264 262 197
158
SnaBI TAC'GTA
Cuts at: 493 4641 493
Size: 4148 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6850
SphI G_CATG'C
Cuts at: 1132 4208 1132
Size: 3076 3774
SspI AAT'ATT
Cuts at: 5 52 4774 5
Size: 47 4722 2081
Fragments arranged by size:
4722 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6850
StyI C'CwwG_G
Cuts at: 513 1065 2004 3012 4289 513
Size: 552 939 1008 1277 3074
Fragments arranged by size:
3074 1277 1008 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3272 4877 4894 5047 5232 5677 325
Size: 1255 1692 1605 17 153 185 445 1498
Fragments arranged by size:
1692 1605 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2331 2546 2944
Size: 274 80 33 51 1443 354 215 398
Cuts at: 2944 3149 3361 3485 3529 5096 6808 96
Size: 205 212 124 44 1567 1712 138
Fragments arranged by size:
1712 1567 1443 398 354 274 215 212
205 138 124 80 51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 6810 6842 2626
Size: 32 4152 32 2634
Fragments arranged by size:
4152 2634 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3188 1316
Size: 1872 4978
XbaI T'CTAG_A
Cuts at: 4186 4186
Size: 6850
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6850
XmaI C'CCGG_G
Cuts at: 1870 4175 1870
Size: 2305 4545
XmnI GAAnn'nnTTC
Cuts at: 2794 3251 3503 3587 3671 4048 4091 4979 2794
Size: 457 252 84 84 377 43 888 4665
Fragments arranged by size:
4665 888 457 377 252 84 84 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4658 276
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI