Restriction map for
pFN21AB6203
(Circular) (Six-base) MAPSORT of: pFN21AB6203.seq Check: 7381 from: 1 to: 6694
pFN21AB6203 6694 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3358 3358
Size: 6694
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4504 278
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4037 1937
Size: 2100 4594
Acc65I G'GTAC_C
Cuts at: 4015 4015
Size: 6694
AclI AA'CG_TT
Cuts at: 1563 4821 5194 1563
Size: 3258 373 3063
Fragments arranged by size:
3258 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2107 2984 3152 3683 4757 6141 6409 1632
Size: 475 877 168 531 1074 1384 268 1917
Fragments arranged by size:
1917 1384 1074 877 531 475 268 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6497
AflIII A'CryG_T
Cuts at: 1203 2541 2823 2931 3151 5593 6570 1203
Size: 1338 282 108 220 2442 977 1327
Fragments arranged by size:
2442 1338 1327 977 282 220 108
AgeI A'CCGG_T
Cuts at: 5525 5525
Size: 6694
AhdI GACnn_n'nnGTC
Cuts at: 2116 3403 5423 2116
Size: 1287 2020 3387
Fragments arranged by size:
3387 2020 1287
AleI CACnn'nnGTG
Cuts at: 1166 2339 3947 1166
Size: 1173 1608 3913
Fragments arranged by size:
3913 1608 1173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3965 1184
Size: 2781 3913
AlwNI CAG_nnn'CTG
Cuts at: 3343 3492 4437 6009 3343
Size: 149 945 1572 4028
Fragments arranged by size:
4028 1572 945 149
ApaI G_GGCC'C
Cuts at: 2432 3192 3816 2432
Size: 760 624 5310
Fragments arranged by size:
5310 760 624
ApaLI G'TGCA_C
Cuts at: 3883 3932 4751 5907 3883
Size: 49 819 1156 4670
Fragments arranged by size:
4670 1156 819 49
ApoI r'AATT_y
Cuts at: 1493 4003 4229 4381 6490 1493
Size: 2510 226 152 2109 1697
Fragments arranged by size:
2510 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5248 160
Size: 872 4216 1606
Fragments arranged by size:
4216 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
AvaI C'yCGr_G
Cuts at: 1870 1943 2788 2843 3183 4019 1870
Size: 73 845 55 340 836 4545
Fragments arranged by size:
4545 845 836 340 73 55
BamHI G'GATC_C
Cuts at: 4024 4391 4024
Size: 367 6327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2444 2884 3941 3988
Size: 325 217 627 282 375 440 1057 47
Cuts at: 3988 4015 5470 618
Size: 27 1455 1842
Fragments arranged by size:
1842 1455 1057 627 440 375 325 282
217 47 27
BanII G_rGCy'C
Cuts at: 1391 2432 2796 2841 3192 3245 3816 4013 1391
Size: 1041 364 45 351 53 571 197 4072
Fragments arranged by size:
4072 1041 571 364 351 197 53 45
BbeI G_GCGC'C
Cuts at: 2888 2888
Size: 6694
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2535 2573 2866 3097 928
Size: 971 636 38 293 231 4525
Fragments arranged by size:
4525 971 636 293 231 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2471 2505 4885 4919 1903
Size: 34 534 34 2380 34 3678
Fragments arranged by size:
3678 2380 534 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4586 5796 4586
Size: 1210 5484
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6694
BfrBI ATG'CAT
Cuts at: 3639 3639
Size: 6694
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3811 5305 136
Size: 107 122 71 631 605 2139 1494 1525
Fragments arranged by size:
2139 1525 1494 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6689 6689
Size: 6694
BlpI GC'TnA_GC
Cuts at: 4111 4111
Size: 6694
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2432 3192 3364 3816 3887 3936 3993
Size: 282 360 760 172 452 71 49 57
Cuts at: 3993 4755 5911 1790
Size: 762 1156 2573
Fragments arranged by size:
2573 1156 762 760 452 360 282 172
71 57 49
BmgBI CAC'GTC
Cuts at: 1318 2118 2826 1318
Size: 800 708 5186
Fragments arranged by size:
5186 800 708
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3242 3881 4425
Size: 416 53 83 399 24 1825 639 544
Cuts at: 4425 5383 442
Size: 958 1753
Fragments arranged by size:
1825 1753 958 639 544 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2495 2495
Size: 6694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2855 3860 3863 3884 5354 1239
Size: 918 698 1005 3 21 1470 2579
Fragments arranged by size:
2579 1470 1005 918 698 21 3
Bpu10I CC'TnA_GC
Cuts at: 2558 3431 2558
Size: 873 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4819 5684 5982 4819
Size: 865 298 5531
Fragments arranged by size:
5531 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5357 882
Size: 617 3858 2219
Fragments arranged by size:
3858 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2611 2755 3418 4485 493
Size: 711 1407 144 663 1067 2702
Fragments arranged by size:
2702 1407 1067 711 663 144
BsaBI GATnn'nnATC
Cuts at: 6688 6688
Size: 6694
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2885 4501 4883 5518
Size: 53 83 186 936 1352 1616 382 635
Cuts at: 5518 275
Size: 1451
Fragments arranged by size:
1616 1451 1352 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5127 5525 5799 5946 6357 5127
Size: 398 274 147 411 5464
Fragments arranged by size:
5464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2589 2619 3836 3861 3866
Size: 30 165 30 1186 30 1217 25 5
Cuts at: 3866 3891 1178
Size: 25 3981
Fragments arranged by size:
3981 1217 1186 165 30 30 30 25
25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3035 3038 3857 1186
Size: 1849 3 819 4023
Fragments arranged by size:
4023 1849 819 3
BseYI C'CCAG_C
Cuts at: 2065 2910 3277 3311 3363 3673 5897 6393 2065
Size: 845 367 34 52 310 2224 496 2366
Fragments arranged by size:
2366 2224 845 496 367 310 52 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2098 2698 2956 3392 1163
Size: 573 362 600 258 436 4465
Fragments arranged by size:
4465 600 573 436 362 258
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4144 4905 5933 1540
Size: 275 186 1828 315 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 315 275 186
BsiHKAI G_wGCw'C
Cuts at: 2564 2607 2841 3887 3936 4755 4840 5911 2564
Size: 43 234 1046 49 819 85 1071 3347
Fragments arranged by size:
3347 1071 1046 819 234 85 49 43
BsmI GAATG_Cn'
Cuts at: 4205 4298 4205
Size: 93 6601
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2432 2564 2607 2796 2841 3192
Size: 399 282 360 132 43 189 45 351
Cuts at: 3192 3245 3364 3816 3887 3936 3993 4013 4755
Size: 53 119 452 71 49 57 20 742
Cuts at: 4755 4840 5911 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 360 351 282
189 132 119 85 71 57 53 49
45 43 20
BspEI T'CCGG_A
Cuts at: 6357 6357
Size: 6694
BspHI T'CATG_A
Cuts at: 2895 4583 6313 2895
Size: 1688 1730 3276
Fragments arranged by size:
3276 1730 1688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 3358 4049 844
Size: 1697 817 691 3489
Fragments arranged by size:
3489 1697 817 691
BsrBI CCG'CTC
Cuts at: 1394 1823 4581 1394
Size: 429 2758 3507
Fragments arranged by size:
3507 2758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3322 5189 5363 65
Size: 1183 2074 1867 174 1396
Fragments arranged by size:
2074 1867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2375 3324 5338 5525 1700
Size: 110 565 949 2014 187 2869
Fragments arranged by size:
2869 2014 949 565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6694
BssSI C'ACGA_G
Cuts at: 4754 5766 4754
Size: 1012 5682
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6694
BstBI TT'CG_AA
Cuts at: 3549 5498 3549
Size: 1949 4745
BstEII G'GTnAC_C
Cuts at: 1184 2230 1184
Size: 1046 5648
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 2455
Size: 6694
BstYI r'GATC_y
Cuts at: 1972 4024 4391 4776 4793 6245 6331 6343 6689
Size: 2052 367 385 17 1452 86 12 346
Cuts at: 6689 1972
Size: 1977
Fragments arranged by size:
2052 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2106 3018 2106
Size: 912 5782
BtgI C'CryG_G
Cuts at: 513 1065 2004 2337 2493 3099 3408 3529 513
Size: 552 939 333 156 606 309 121 3678
Fragments arranged by size:
3678 939 606 552 333 309 156 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5955
Fragments arranged by size:
5955 683 56
BtsI GCAGTG_nn'
Cuts at: 3934 4210 5004 5024 3934
Size: 276 794 20 5604
Fragments arranged by size:
5604 794 276 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6659
DraI TTT'AAA
Cuts at: 3998 3998
Size: 6694
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2870 5701 809
Size: 507 1554 2831 1802
Fragments arranged by size:
2831 1802 1554 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2613 2982
Size: 54 1425 50 275 18 420 363 369
Cuts at: 2982 3477 3681 3826 4141 5030 6659 8
Size: 495 204 145 315 889 1629 43
Fragments arranged by size:
1629 1425 889 495 420 369 363 315
275 204 145 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 4141 1537
Size: 275 2014 315 4090
Fragments arranged by size:
4090 2014 315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3296 3719 4624 1353
Size: 1943 423 905 3423
Fragments arranged by size:
3423 1943 905 423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3755 5281 5655 5801 387
Size: 1064 138 2166 1526 374 146 1280
Fragments arranged by size:
2166 1526 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2839 2839
Size: 6694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2157 2855 2984 3152 3683 3860
Size: 393 475 50 698 129 168 531 177
Cuts at: 3860 3863 3884 4757 5354 6141 6409 1239
Size: 3 21 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 698 597 531 475 393
268 177 168 129 50 21 3
EcoO109I rG'GnC_Cy
Cuts at: 2415 2428 2429 2693 2761 3188 3189 3340 3376
Size: 13 1 264 68 427 1 151 36
Cuts at: 3376 3489 3538 2415
Size: 113 49 5571
Fragments arranged by size:
5571 427 264 151 113 68 49 36
13 1 1
EcoRI G'AATT_C
Cuts at: 4003 4381 4003
Size: 378 6316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 6694
FspI TGC'GCA
Cuts at: 2359 3953 5200 2359
Size: 1594 1247 3853
Fragments arranged by size:
3853 1594 1247
HaeII r_GCGC'y
Cuts at: 1678 2888 5841 1678
Size: 1210 2953 2531
Fragments arranged by size:
2953 2531 1210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 3389 3421 5335
Size: 32 135 32 530 32 1255 32 1914
Cuts at: 5335 5367 5409 5441 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1914 1255 530 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4038 4284 669
Size: 988 281 2100 246 3079
Fragments arranged by size:
3079 2100 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3744 748
Size: 2996 3698
HpaI GTT'AAC
Cuts at: 4284 4284
Size: 6694
KasI G'GCGC_C
Cuts at: 2884 2884
Size: 6694
KpnI G_GTAC'C
Cuts at: 4019 4019
Size: 6694
MfeI C'AATT_G
Cuts at: 4293 4293
Size: 6694
MluI A'CGCG_T
Cuts at: 2931 3151 6570 2931
Size: 220 3419 3055
Fragments arranged by size:
3419 3055 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2853 5808 5992 1305
Size: 1548 2955 184 2007
Fragments arranged by size:
2955 2007 1548 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 2984 3479 10
Size: 54 1425 343 783 369 495 3225
Fragments arranged by size:
3225 1425 783 495 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 3773 3947 4652 5011 5170 518
Size: 648 1173 1434 174 705 359 159 2042
Fragments arranged by size:
2042 1434 1173 705 648 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 2339 3277 3303 3831 4110 4787 5935 6180
Size: 733 938 26 528 279 677 1148 245
Cuts at: 6180 1606
Size: 2120
Fragments arranged by size:
2120 1148 938 733 677 528 279 245
26
NaeI GCC'GGC
Cuts at: 3326 3326
Size: 6694
NarI GG'CG_CC
Cuts at: 2885 2885
Size: 6694
NcoI C'CATG_G
Cuts at: 513 1065 2004 2493 513
Size: 552 939 489 4714
Fragments arranged by size:
4714 939 552 489
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6694
NgoMIV G'CCGG_C
Cuts at: 3324 3324
Size: 6694
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6694
NotI GC'GGCC_GC
Cuts at: 4141 4141
Size: 6694
NsiI A_TGCA'T
Cuts at: 3641 3641
Size: 6694
NspI r_CATG'y
Cuts at: 1132 1229 2545 3772 4052 5597 1132
Size: 97 1316 1227 280 1545 2229
Fragments arranged by size:
2229 1545 1316 1227 280 97
PasI CC'CwG_GG
Cuts at: 1348 2011 2141 2219 1348
Size: 663 130 78 5823
Fragments arranged by size:
5823 663 130 78
PciI A'CATG_T
Cuts at: 2541 5593 2541
Size: 3052 3642
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2599 3170 1108
Size: 1491 571 4632
Fragments arranged by size:
4632 1491 571
PfoI T'CCnGG_A
Cuts at: 2772 3507 2772
Size: 735 5959
PmeI GTTT'AAAC
Cuts at: 3998 3998
Size: 6694
PmlI CAC'GTG
Cuts at: 2611 3418 2611
Size: 807 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4763 4795 6276 6308 4763
Size: 32 1481 32 5149
Fragments arranged by size:
5149 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2415 2693 3489 2415
Size: 278 796 5620
Fragments arranged by size:
5620 796 278
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6694
PsiI TTA'TAA
Cuts at: 4264 4264
Size: 6694
PspOMI G'GGCC_C
Cuts at: 2428 3188 3812 2428
Size: 760 624 5310
Fragments arranged by size:
5310 760 624
PspXI vC'TCGA_Gb
Cuts at: 3183 3183
Size: 6694
PstI C_TGCA'G
Cuts at: 830 2096 2538 3348 4046 830
Size: 1266 442 810 698 3478
Fragments arranged by size:
3478 1266 810 698 442
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6694
PvuII CAG'CTG
Cuts at: 3303 3303
Size: 6694
SacI G_AGCT'C
Cuts at: 2841 2841
Size: 6694
SacII CC_GC'GG
Cuts at: 2340 2340
Size: 6694
SalI G'TCGA_C
Cuts at: 1936 4036 1936
Size: 2100 4594
SanDI GG'GwC_CC
Cuts at: 2693 2693
Size: 6694
SapI GCTCTTCn'nnn_
Cuts at: 3719 3719
Size: 6694
SbfI CC_TGCA'GG
Cuts at: 2538 3348 4046 2538
Size: 810 698 5186
Fragments arranged by size:
5186 810 698
ScaI AGT'ACT
Cuts at: 4942 6654 4942
Size: 1712 4982
SfcI C'TryA_G
Cuts at: 826 1045 2092 2534 3344 4042 5177 5858 6049
Size: 219 1047 442 810 698 1135 681 191
Cuts at: 6049 826
Size: 1471
Fragments arranged by size:
1471 1135 1047 810 698 681 442 219
191
SfoI GGC'GCC
Cuts at: 2886 2886
Size: 6694
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6694
SmaI CCC'GGG
Cuts at: 1872 2790 4021 1872
Size: 918 1231 4545
Fragments arranged by size:
4545 1231 918
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3183 4798 5699 5961 820
Size: 197 926 1240 1615 901 262 1553
Fragments arranged by size:
1615 1553 1240 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4485 493
Size: 3992 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6694
SphI G_CATG'C
Cuts at: 1132 4052 1132
Size: 2920 3774
SspI AAT'ATT
Cuts at: 5 52 4618 5
Size: 47 4566 2081
Fragments arranged by size:
4566 2081 47
StuI AGG'CCT
Cuts at: 1517 3129 1517
Size: 1612 5082
StyI C'CwwG_G
Cuts at: 513 1065 2004 2493 2574 3116 4133 513
Size: 552 939 489 81 542 1017 3074
Fragments arranged by size:
3074 1017 939 552 542 489 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 2924 4721 4738 4891 5076 5521
Size: 1255 483 861 1797 17 153 185 445
Cuts at: 5521 325
Size: 1498
Fragments arranged by size:
1797 1498 1255 861 483 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4940 6652 96
Size: 274 80 33 51 1443 2963 1712 138
Fragments arranged by size:
2963 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2589 2621 3026 3058 3836 3861 3868 3893 6654
Size: 32 405 32 778 25 7 25 2761
Cuts at: 6654 6686 2589
Size: 32 2597
Fragments arranged by size:
2761 2597 778 405 32 32 32 25
25 7
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6694
XbaI T'CTAG_A
Cuts at: 4030 4030
Size: 6694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2209 2623 2815 3920 2209
Size: 414 192 1105 4983
Fragments arranged by size:
4983 1105 414 192
XhoI C'TCGA_G
Cuts at: 1943 3183 1943
Size: 1240 5454
XmaI C'CCGG_G
Cuts at: 1870 2788 4019 1870
Size: 918 1231 4545
Fragments arranged by size:
4545 1231 918
XmnI GAAnn'nnTTC
Cuts at: 3461 4823 3461
Size: 1362 5332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4502 276
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI PacI
PsrI RsrII SexAI SfiI SrfI SwaI