Restriction map for
pFN21AB6202
(Circular) (Six-base) MAPSORT of: pFN21AB6202.seq Check: 6278 from: 1 to: 6838
pFN21AB6202 6838 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4181 1937
Size: 2244 4594
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2095 2218 2295 3124 4901 6285 6553 1632
Size: 463 123 77 829 1777 1384 268 1917
Fragments arranged by size:
1917 1777 1384 829 463 268 123 77
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 5737 6714 1203
Size: 4534 977 1327
Fragments arranged by size:
4534 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 3441 3750 1166
Size: 2275 309 4254
Fragments arranged by size:
4254 2275 309
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6838
AlwNI CAG_nnn'CTG
Cuts at: 2044 2134 3525 4581 6153 2044
Size: 90 1391 1056 1572 2729
Fragments arranged by size:
2729 1572 1391 1056 90
ApaLI G'TGCA_C
Cuts at: 3769 4895 6051 3769
Size: 1126 1156 4556
Fragments arranged by size:
4556 1156 1126
ApoI r'AATT_y
Cuts at: 1493 2020 2460 2817 2901 3237 3489 3573 3657
Size: 527 440 357 84 336 252 84 84
Cuts at: 3657 3741 3805 3993 4147 4373 4525 6634 1493
Size: 84 64 188 154 226 152 2109 1697
Fragments arranged by size:
2109 1697 527 440 357 336 252 226
188 154 152 84 84 84 84 64
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 4163 1870
Size: 73 2220 4545
Fragments arranged by size:
4545 2220 73
BamHI G'GATC_C
Cuts at: 4168 4535 4168
Size: 367 6471
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4159 5614 618
Size: 325 217 627 2372 1455 1842
Fragments arranged by size:
2372 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 4157 1391
Size: 790 1976 4072
Fragments arranged by size:
4072 1976 790
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5867
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5029 5063 1903
Size: 34 50 34 3008 34 3678
Fragments arranged by size:
3678 3008 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4730 5940 4730
Size: 1210 5628
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6838
BfrBI ATG'CAT
Cuts at: 3263 3649 3263
Size: 386 6452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5449 136
Size: 107 122 71 631 605 3777 1525
Fragments arranged by size:
3777 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6833 6833
Size: 6838
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 3773 4899 6055 1790
Size: 1983 1126 1156 2573
Fragments arranged by size:
2573 1983 1156 1126
BmgBI CAC'GTC
Cuts at: 1318 2098 1318
Size: 780 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4569 5527 442
Size: 416 53 83 399 24 3152 958 1753
Fragments arranged by size:
3152 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3188
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 3603 5498 1239
Size: 956 1408 1895 2579
Fragments arranged by size:
2579 1895 1408 956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2789 3962 4963 5828 6126 2789
Size: 1173 1001 865 298 3501
Fragments arranged by size:
3501 1173 1001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5501 882
Size: 617 4002 2219
Fragments arranged by size:
4002 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4629 493
Size: 711 3425 2702
Fragments arranged by size:
3425 2702 711
BsaBI GATnn'nnATC
Cuts at: 3480 6832 3480
Size: 3352 3486
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4645 5027 5662 275
Size: 53 83 186 936 3112 382 635 1451
Fragments arranged by size:
3112 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3665 5271 5669 5943 6090 6501 3665
Size: 1606 398 274 147 411 4002
Fragments arranged by size:
4002 1606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6613
Fragments arranged by size:
6613 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2282 1186
Size: 949 147 5742
Fragments arranged by size:
5742 949 147
BseYI C'CCAG_C
Cuts at: 6041 6537 6041
Size: 496 6342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6265
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 3773 4899 4984 6055 2181
Size: 1592 1126 85 1071 2964
Fragments arranged by size:
2964 1592 1126 1071 85
BsmI GAATG_Cn'
Cuts at: 2244 3185 3362 3521 3614 3941 4349 4442 2244
Size: 941 177 159 93 327 408 93 4640
Fragments arranged by size:
4640 941 408 327 177 159 93 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 3773 4157 4899 4984 6055 1391
Size: 399 391 1592 384 742 85 1071 2174
Fragments arranged by size:
2174 1592 1071 742 399 391 384 85
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 4727 6457 4727
Size: 1730 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3789 4193 844
Size: 2945 404 3489
Fragments arranged by size:
3489 2945 404
BsrBI CCG'CTC
Cuts at: 1394 1823 4725 1394
Size: 429 2902 3507
Fragments arranged by size:
3507 2902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2662 5333 5507 65
Size: 1183 1414 2671 174 1396
Fragments arranged by size:
2671 1414 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5482 5669 1700
Size: 110 3672 187 2869
Fragments arranged by size:
3672 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 2554 4898 5910 2554
Size: 2344 1012 3482
Fragments arranged by size:
3482 2344 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3609 794
Size: 2815 4023
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 2765 1184
Size: 1581 5257
BstXI CCAn_nnnn'nTGG
Cuts at: 2930 3107 3443 2930
Size: 177 336 6325
Fragments arranged by size:
6325 336 177
BstYI r'GATC_y
Cuts at: 1972 2066 4168 4535 4920 4937 6389 6475 6487
Size: 94 2102 367 385 17 1452 86 12
Cuts at: 6487 6833 1972
Size: 346 1977
Fragments arranged by size:
2102 1977 1452 385 367 346 94 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2037 2037
Size: 6838
BtgI C'CryG_G
Cuts at: 513 1065 2103 513
Size: 552 1038 5248
Fragments arranged by size:
5248 1038 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 2205 3190 3274 3526 3610 3694 4354 5148 5168
Size: 985 84 252 84 84 660 794 20
Cuts at: 5168 2205
Size: 3875
Fragments arranged by size:
3875 985 794 660 252 84 84 84
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3004 3039 529
Size: 35 2440 35 4328
Fragments arranged by size:
4328 2440 35 35
DraI TTT'AAA
Cuts at: 4142 4142
Size: 6838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2788 4285 5174
Size: 54 1425 50 275 18 958 1497 889
Cuts at: 5174 6803 8
Size: 1629 43
Fragments arranged by size:
1629 1497 1425 958 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 2719 3143 4768 1353
Size: 845 521 424 1625 3423
Fragments arranged by size:
3423 1625 845 521 424
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2179 2179
Size: 6838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2195 2218 2295 3124 3603 4901
Size: 393 463 100 23 77 829 479 1298
Cuts at: 4901 5498 6285 6553 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1298 829 787 597 479 463 393
268 100 77 23
EcoO109I rG'GnC_Cy
Cuts at: 2041 2533 2559 2041
Size: 492 26 6320
Fragments arranged by size:
6320 492 26
EcoRI G'AATT_C
Cuts at: 2901 3489 3657 3741 3993 4147 4525 2901
Size: 588 168 84 252 154 378 5214
Fragments arranged by size:
5214 588 378 252 168 154 84
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 5985 1678
Size: 4307 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3118 3150 3382 3414 3886
Size: 32 135 32 1546 32 232 32 472
Cuts at: 3886 3918 5479 5511 5553 5585 1373
Size: 32 1561 32 42 32 2626
Fragments arranged by size:
2626 1561 1546 472 232 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 4182 4428 669
Size: 988 281 1362 882 246 3079
Fragments arranged by size:
3079 1362 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 2346 748
Size: 1598 5240
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 2518 4437 2518
Size: 1919 4919
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5952 6136 1305
Size: 4647 184 2007
Fragments arranged by size:
4647 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2790 10
Size: 54 1425 343 958 4058
Fragments arranged by size:
4058 1425 958 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2928 2994 3105 3246 3441 3750 4796
Size: 648 1762 66 111 141 195 309 1046
Cuts at: 4796 5155 5314 518
Size: 359 159 2042
Fragments arranged by size:
2042 1762 1046 648 359 309 195 159
141 111 66
MspA1I CmG'CkG
Cuts at: 1606 4254 4931 6079 6324 1606
Size: 2648 677 1148 245 2120
Fragments arranged by size:
2648 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6286
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NsiI A_TGCA'T
Cuts at: 3265 3651 3265
Size: 386 6452
NspI r_CATG'y
Cuts at: 1132 1229 3267 3649 4196 5741 1132
Size: 97 2038 382 547 1545 2229
Fragments arranged by size:
2229 2038 1545 547 382 97
PasI CC'CwG_GG
Cuts at: 1348 2537 1348
Size: 1189 5649
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6838
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2533 2041
Size: 492 6346
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PstI C_TGCA'G
Cuts at: 830 2729 4190 830
Size: 1899 1461 3478
Fragments arranged by size:
3478 1899 1461
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
SacI G_AGCT'C
Cuts at: 2181 2181
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SapI GCTCTTCn'nnn_
Cuts at: 2719 2719
Size: 6838
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 5086 6798 5086
Size: 1712 5126
SfcI C'TryA_G
Cuts at: 826 1045 2182 2725 4186 5321 6002 6193 826
Size: 219 1137 543 1461 1135 681 191 1471
Fragments arranged by size:
1471 1461 1137 1135 681 543 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 4165 1872
Size: 2293 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2804 3977 4942 5843 6105 820
Size: 197 926 861 1173 965 901 262 1553
Fragments arranged by size:
1553 1173 965 926 901 861 262 197
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 3267 3649 4196 1132
Size: 2135 382 547 3774
Fragments arranged by size:
3774 2135 547 382
SspI AAT'ATT
Cuts at: 5 52 2737 4762 5
Size: 47 2685 2025 2081
Fragments arranged by size:
2685 2081 2025 47
StuI AGG'CCT
Cuts at: 1517 3368 3788 3844 4040 1517
Size: 1851 420 56 196 4315
Fragments arranged by size:
4315 1851 420 196 56
StyI C'CwwG_G
Cuts at: 513 1065 4277 513
Size: 552 3212 3074
Fragments arranged by size:
3212 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2885 3557 4865 4882 5035 5220 5665
Size: 1255 1305 672 1308 17 153 185 445
Cuts at: 5665 325
Size: 1498
Fragments arranged by size:
1498 1308 1305 1255 672 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5084 6796 96
Size: 274 80 33 51 1443 3107 1712 138
Fragments arranged by size:
3107 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6798 6830 6798
Size: 32 6806
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 4163 1870
Size: 2293 4545
XmnI GAAnn'nnTTC
Cuts at: 3452 3620 3704 3788 3956 4967 3452
Size: 168 84 84 168 1011 5323
Fragments arranged by size:
5323 1011 168 168 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI