Restriction map for
pFN21AB6196
(Circular) (Six-base) MAPSORT of: pFN21AB6196.seq Check: 712 from: 1 to: 6823
pFN21AB6196 6823 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3337 3337
Size: 6823
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2691 4166 1937
Size: 754 1475 4594
Fragments arranged by size:
4594 1475 754
Acc65I G'GTAC_C
Cuts at: 4144 4144
Size: 6823
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 2723 3042 3818 4886 6270 6538 1632
Size: 1049 42 319 776 1068 1384 268 1917
Fragments arranged by size:
1917 1384 1068 1049 776 319 268 42
AfeI AGC'GCT
Cuts at: 2374 2374
Size: 6823
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6626
AflIII A'CryG_T
Cuts at: 1203 5722 6699 1203
Size: 4519 977 1327
Fragments arranged by size:
4519 1327 977
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 5552 5552
Size: 6823
AleI CACnn'nnGTG
Cuts at: 1166 2723 1166
Size: 1557 5266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6823
AlwNI CAG_nnn'CTG
Cuts at: 2313 2493 2984 2993 2996 3101 3194 3475 3730
Size: 180 491 9 3 105 93 281 255
Cuts at: 3730 4070 4566 6138 2313
Size: 340 496 1572 2998
Fragments arranged by size:
2998 1572 496 491 340 281 255 180
105 93 9 3
ApaI G_GGCC'C
Cuts at: 3510 3510
Size: 6823
ApaLI G'TGCA_C
Cuts at: 4880 6036 4880
Size: 1156 5667
ApoI r'AATT_y
Cuts at: 1493 3118 4132 4358 4510 6619 1493
Size: 1625 1014 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 1014 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5377 160
Size: 872 4345 1606
Fragments arranged by size:
4345 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 2507 4148 1870
Size: 73 564 1641 4545
Fragments arranged by size:
4545 1641 564 73
BamHI G'GATC_C
Cuts at: 4153 4520 4153
Size: 367 6456
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2768 4144 5599 618
Size: 325 217 627 300 681 1376 1455 1842
Fragments arranged by size:
1842 1455 1376 681 627 325 300 217
BanII G_rGCy'C
Cuts at: 1391 2181 3425 3510 3763 4142 1391
Size: 790 1244 85 253 379 4072
Fragments arranged by size:
4072 1244 790 379 253 85
BbeI G_GCGC'C
Cuts at: 2772 2772
Size: 6823
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3687 4099 928
Size: 971 1788 412 3652
Fragments arranged by size:
3652 1788 971 412
BbvCI CC'TCA_GC
Cuts at: 3227 3227
Size: 6823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5014 5048 1903
Size: 34 3077 34 3678
Fragments arranged by size:
3678 3077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4715 5925 4715
Size: 1210 5613
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6823
BfrBI ATG'CAT
Cuts at: 3562 3562
Size: 6823
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2597 5434 136
Size: 107 122 71 631 605 925 2837 1525
Fragments arranged by size:
2837 1525 925 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6818 6818
Size: 6823
BlpI GC'TnA_GC
Cuts at: 4240 4240
Size: 6823
Bme1580I G_kGCm'C
Cuts at: 1790 3510 4884 6040 1790
Size: 1720 1374 1156 2573
Fragments arranged by size:
2573 1720 1374 1156
BmgBI CAC'GTC
Cuts at: 1318 2070 1318
Size: 752 6071
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2531 2774 3642
Size: 416 53 83 399 24 1114 243 868
Cuts at: 3642 4554 5512 442
Size: 912 958 1753
Fragments arranged by size:
1753 1114 958 912 868 416 399 243
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 2791 3171 3362 3639 5483 1239
Size: 1493 59 380 191 277 1844 2579
Fragments arranged by size:
2579 1844 1493 380 277 191 59
Bpu10I CC'TnA_GC
Cuts at: 3227 3227
Size: 6823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3747 3860 3956 4948 5813 6111 3747
Size: 113 96 992 865 298 4459
Fragments arranged by size:
4459 992 865 298 113 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2816 2942 3572 5486 882
Size: 617 1317 126 630 1914 2219
Fragments arranged by size:
2219 1914 1317 630 617 126
BsaAI yAC'GTr
Cuts at: 493 1204 4614 493
Size: 711 3410 2702
Fragments arranged by size:
3410 2702 711
BsaBI GATnn'nnATC
Cuts at: 6817 6817
Size: 6823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2385 2769 4630
Size: 53 83 186 936 614 238 384 1861
Cuts at: 4630 5012 5647 275
Size: 382 635 1451
Fragments arranged by size:
1861 1451 936 635 614 384 382 238
186 83 53
BsaWI w'CCGG_w
Cuts at: 5256 5654 5928 6075 6486 5256
Size: 398 274 147 411 5593
Fragments arranged by size:
5593 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2104 2134 1178
Size: 30 165 30 701 30 5867
Fragments arranged by size:
5867 701 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2071 3359 1186
Size: 885 1288 4650
Fragments arranged by size:
4650 1288 885
BseYI C'CCAG_C
Cuts at: 2037 6026 6522 2037
Size: 3989 496 2338
Fragments arranged by size:
3989 2338 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2824 1163
Size: 573 1088 5162
Fragments arranged by size:
5162 1088 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2334 4273 5034 6062 1540
Size: 275 186 333 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 333 275 186
BsiHKAI G_wGCw'C
Cuts at: 3425 3763 4884 4969 6040 3425
Size: 338 1121 85 1071 4208
Fragments arranged by size:
4208 1121 1071 338 85
BsmI GAATG_Cn'
Cuts at: 2923 4334 4427 2923
Size: 1411 93 5319
Fragments arranged by size:
5319 1411 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2124 3938 2124
Size: 1814 5009
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 3425 3510 3763 4142 4884 4969
Size: 399 391 1244 85 253 379 742 85
Cuts at: 4969 6040 1391
Size: 1071 2174
Fragments arranged by size:
2174 1244 1071 742 399 391 379 253
85 85
BspEI T'CCGG_A
Cuts at: 6486 6486
Size: 6823
BspHI T'CATG_A
Cuts at: 3532 4712 6442 3532
Size: 1180 1730 3913
Fragments arranged by size:
3913 1730 1180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3337 4178 844
Size: 2493 841 3489
Fragments arranged by size:
3489 2493 841
BsrBI CCG'CTC
Cuts at: 1394 1823 4710 1394
Size: 429 2887 3507
Fragments arranged by size:
3507 2887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2740 3033 3213 3234 5318 5492 65
Size: 1183 1492 293 180 21 2084 174 1396
Fragments arranged by size:
2084 1492 1396 1183 293 180 174 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2222 2417 2722 3527 5467 5654
Size: 110 204 208 195 305 805 1940 187
Cuts at: 5654 1700
Size: 2869
Fragments arranged by size:
2869 1940 805 305 208 204 195 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 4883 5895 4883
Size: 1012 5811
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2313 2493 2984 2993 2996 3017 794
Size: 1519 180 491 9 3 21 4600
Fragments arranged by size:
4600 1519 491 180 21 9 3
BstBI TT'CG_AA
Cuts at: 5627 5627
Size: 6823
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6823
BstXI CCAn_nnnn'nTGG
Cuts at: 3452 3452
Size: 6823
BstYI r'GATC_y
Cuts at: 1972 2578 4153 4520 4905 4922 6374 6460 6472
Size: 606 1575 367 385 17 1452 86 12
Cuts at: 6472 6818 1972
Size: 346 1977
Fragments arranged by size:
1977 1575 1452 606 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2756 2756
Size: 6823
BtgI C'CryG_G
Cuts at: 513 1065 2004 3201 513
Size: 552 939 1197 4135
Fragments arranged by size:
4135 1197 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2167 3365 487
Size: 683 56 941 1198 3945
Fragments arranged by size:
3945 1198 941 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 3647 4057 4339 5133 5153 2016
Size: 1631 410 282 794 20 3686
Fragments arranged by size:
3686 1631 794 410 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6788
DraI TTT'AAA
Cuts at: 4127 4127
Size: 6823
DraIII CAC_nnn'GTG
Cuts at: 2671 2671
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5830 809
Size: 507 4514 1802
Fragments arranged by size:
4514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2331 3525
Size: 54 1425 50 275 18 375 126 1194
Cuts at: 3525 4270 5159 6788 8
Size: 745 889 1629 43
Fragments arranged by size:
1629 1425 1194 889 745 375 275 126
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2331 4270 1537
Size: 275 519 1939 4090
Fragments arranged by size:
4090 1939 519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2110 3430 3714 4753 1353
Size: 757 1320 284 1039 3423
Fragments arranged by size:
3423 1320 1039 757 284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 5410 5784 5930 387
Size: 1064 138 570 3251 374 146 1280
Fragments arranged by size:
3251 1280 1064 570 374 146 138
EcoICRI GAG'CTC
Cuts at: 3423 3761 3423
Size: 338 6485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2681 2723 2732 2791 3042 3171 3362
Size: 393 1049 42 9 59 251 129 191
Cuts at: 3362 3639 3818 4886 5483 6270 6538 1239
Size: 277 179 1068 597 787 268 1524
Fragments arranged by size:
1524 1068 1049 787 597 393 277 268
251 191 179 129 59 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2639 2639
Size: 6823
EcoRI G'AATT_C
Cuts at: 3118 4132 4510 3118
Size: 1014 378 5431
Fragments arranged by size:
5431 1014 378
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 2376 2772 5970 1678
Size: 698 396 3198 2531
Fragments arranged by size:
3198 2531 698 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2104 2136 3052 3084 3308
Size: 32 135 32 532 32 916 32 224
Cuts at: 3308 3340 3608 3640 3677 3709 5464 5496 5538
Size: 32 268 32 37 32 1755 32 42
Cuts at: 5538 5570 1373
Size: 32 2626
Fragments arranged by size:
2626 1755 916 532 268 224 135 42
37 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4167 4413 669
Size: 988 281 2229 246 3079
Fragments arranged by size:
3079 2229 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 4413 4413
Size: 6823
KasI G'GCGC_C
Cuts at: 2768 2768
Size: 6823
KpnI G_GTAC'C
Cuts at: 4148 4148
Size: 6823
MfeI C'AATT_G
Cuts at: 4422 4422
Size: 6823
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2057 3109 3802 5937 6121 1305
Size: 752 1052 693 2135 184 2007
Fragments arranged by size:
2135 2007 1052 752 693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5001
Fragments arranged by size:
5001 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2723 3206 3450 4781 5140 5299
Size: 648 843 714 483 244 1331 359 159
Cuts at: 5299 518
Size: 2042
Fragments arranged by size:
2042 1331 843 714 648 483 359 244
159
MspA1I CmG'CkG
Cuts at: 1606 2041 2250 2271 2349 2493 2793 2984 2993
Size: 435 209 21 78 144 300 191 9
Cuts at: 2993 4239 4916 6064 6309 1606
Size: 1246 677 1148 245 2120
Fragments arranged by size:
2120 1246 1148 677 435 300 245 209
191 144 78 21 9
NaeI GCC'GGC
Cuts at: 2016 2016
Size: 6823
NarI GG'CG_CC
Cuts at: 2769 2769
Size: 6823
NcoI C'CATG_G
Cuts at: 513 1065 2004 3201 513
Size: 552 939 1197 4135
Fragments arranged by size:
4135 1197 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6823
NgoMIV G'CCGG_C
Cuts at: 2014 2014
Size: 6823
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6823
NotI GC'GGCC_GC
Cuts at: 2331 4270 2331
Size: 1939 4884
NsiI A_TGCA'T
Cuts at: 3564 3564
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 4181 5726 1132
Size: 97 2952 1545 2229
Fragments arranged by size:
2952 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6823
PciI A'CATG_T
Cuts at: 5722 5722
Size: 6823
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6823
PfoI T'CCnGG_A
Cuts at: 2807 2807
Size: 6823
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3864 3896 4892 4924 6405 6437 3864
Size: 32 996 32 1481 32 4250
Fragments arranged by size:
4250 1481 996 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3547 1613
Size: 1934 4889
PsiI TTA'TAA
Cuts at: 4393 4393
Size: 6823
PspOMI G'GGCC_C
Cuts at: 3506 3506
Size: 6823
PstI C_TGCA'G
Cuts at: 830 2989 4175 830
Size: 2159 1186 3478
Fragments arranged by size:
3478 2159 1186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
PvuII CAG'CTG
Cuts at: 2041 2493 2993 2041
Size: 452 500 5871
Fragments arranged by size:
5871 500 452
SacI G_AGCT'C
Cuts at: 3425 3763 3425
Size: 338 6485
SalI G'TCGA_C
Cuts at: 1936 4165 1936
Size: 2229 4594
SapI GCTCTTCn'nnn_
Cuts at: 3430 3430
Size: 6823
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 5071 6783 5071
Size: 1712 5111
SfcI C'TryA_G
Cuts at: 826 1045 2985 4171 5306 5987 6178 826
Size: 219 1940 1186 1135 681 191 1471
Fragments arranged by size:
1940 1471 1186 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2770 2770
Size: 6823
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 2722 1700
Size: 1022 5801
SmaI CCC'GGG
Cuts at: 1872 2509 4150 1872
Size: 637 1641 4545
Fragments arranged by size:
4545 1641 637
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3762 3839 3935 4927 5828 6090
Size: 197 926 1819 77 96 992 901 262
Cuts at: 6090 820
Size: 1553
Fragments arranged by size:
1819 1553 992 926 901 262 197 96
77
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 4181 1132
Size: 3049 3774
SspI AAT'ATT
Cuts at: 5 52 4747 5
Size: 47 4695 2081
Fragments arranged by size:
4695 2081 47
StuI AGG'CCT
Cuts at: 1517 2869 3358 1517
Size: 1352 489 4982
Fragments arranged by size:
4982 1352 489
StyI C'CwwG_G
Cuts at: 513 1065 2004 3201 4262 513
Size: 552 939 1197 1061 3074
Fragments arranged by size:
3074 1197 1061 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3384 4850 4867 5020 5205 5650 325
Size: 1255 1804 1466 17 153 185 445 1498
Fragments arranged by size:
1804 1498 1466 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 3496 5069
Size: 274 80 33 51 1443 543 976 1573
Cuts at: 5069 6781 96
Size: 1712 138
Fragments arranged by size:
1712 1573 1443 976 543 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2872 2904 6783 6815 2872
Size: 32 3879 32 2880
Fragments arranged by size:
3879 2880 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2116 1316
Size: 800 6023
XbaI T'CTAG_A
Cuts at: 2855 4159 2855
Size: 1304 5519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3241 3241
Size: 6823
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 2507 4148 1870
Size: 637 1641 4545
Fragments arranged by size:
4545 1641 637
XmnI GAAnn'nnTTC
Cuts at: 2745 2816 4952 2745
Size: 71 2136 4616
Fragments arranged by size:
4616 2136 71
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BplI BsiWI BstZ17I EcoO109I
EcoRV FalI FseI FspAI NruI PacI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI