Restriction map for
pFN21AB6195
(Circular) (Six-base) MAPSORT of: pFN21AB6195.seq Check: 9291 from: 1 to: 6694
pFN21AB6195 6694 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4504 278
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4037 1937
Size: 2100 4594
Acc65I G'GTAC_C
Cuts at: 2122 4015 2122
Size: 1893 4801
AclI AA'CG_TT
Cuts at: 1563 4821 5194 1563
Size: 3258 373 3063
Fragments arranged by size:
3258 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 3183 3711 3716 4757 6141 6409 1632
Size: 697 854 528 5 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1041 854 697 528 268 5
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6497
AflIII A'CryG_T
Cuts at: 1203 5593 6570 1203
Size: 4390 977 1327
Fragments arranged by size:
4390 1327 977
AgeI A'CCGG_T
Cuts at: 5525 5525
Size: 6694
AhdI GACnn_n'nnGTC
Cuts at: 5423 5423
Size: 6694
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2258 1184
Size: 1074 5620
AlwNI CAG_nnn'CTG
Cuts at: 2227 2860 3524 3842 4437 6009 2227
Size: 633 664 318 595 1572 2912
Fragments arranged by size:
2912 1572 664 633 595 318
ApaI G_GGCC'C
Cuts at: 3540 3667 3540
Size: 127 6567
ApaLI G'TGCA_C
Cuts at: 4751 5907 4751
Size: 1156 5538
ApoI r'AATT_y
Cuts at: 1493 3060 4003 4229 4381 6490 1493
Size: 1567 943 226 152 2109 1697
Fragments arranged by size:
2109 1697 1567 943 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5248 160
Size: 872 4216 1606
Fragments arranged by size:
4216 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
AvaI C'yCGr_G
Cuts at: 1870 1943 2152 4019 1870
Size: 73 209 1867 4545
Fragments arranged by size:
4545 1867 209 73
AvrII C'CTAG_G
Cuts at: 3220 3605 3220
Size: 385 6309
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2545 2578 2545
Size: 33 6661
BamHI G'GATC_C
Cuts at: 4024 4391 4024
Size: 367 6327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3502 4015 5470 618
Size: 325 217 627 335 1380 513 1455 1842
Fragments arranged by size:
1842 1455 1380 627 513 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2427 3248 3442 3540 3667 3711 3855 3984
Size: 1036 821 194 98 127 44 144 129
Cuts at: 3984 4013 1391
Size: 29 4072
Fragments arranged by size:
4072 1036 821 194 144 129 127 98
44 29
BbeI G_GCGC'C
Cuts at: 3506 3506
Size: 6694
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 2519 2549 928
Size: 971 192 428 30 5073
Fragments arranged by size:
5073 971 428 192 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4885 4919 1903
Size: 34 2948 34 3678
Fragments arranged by size:
3678 2948 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4586 5796 4586
Size: 1210 5484
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6694
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2957 2966 3437
Size: 107 122 71 631 605 1285 9 471
Cuts at: 3437 3786 5305 136
Size: 349 1519 1525
Fragments arranged by size:
1525 1519 1285 631 605 471 349 122
107 71 9
BglII A'GATC_T
Cuts at: 2340 3014 6689 2340
Size: 674 3675 2345
Fragments arranged by size:
3675 2345 674
BlpI GC'TnA_GC
Cuts at: 3885 4111 3885
Size: 226 6468
Bme1580I G_kGCm'C
Cuts at: 1790 2809 3207 3540 3667 4755 5911 1790
Size: 1019 398 333 127 1088 1156 2573
Fragments arranged by size:
2573 1156 1088 1019 398 333 127
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6694
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 2526 3830
Size: 416 53 83 399 24 602 507 1304
Cuts at: 3830 4425 5383 442
Size: 595 958 1753
Fragments arranged by size:
1753 1304 958 602 595 507 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2506 3874 2284
Size: 222 1368 5104
Fragments arranged by size:
5104 1368 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2476 2498 2983 3062 3138 3320 5354
Size: 941 296 22 485 79 76 182 2034
Cuts at: 5354 1239
Size: 2579
Fragments arranged by size:
2579 2034 941 485 296 182 79 76
22
Bpu10I CC'TnA_GC
Cuts at: 3865 3865
Size: 6694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3869 4819 5684 5982 3869
Size: 950 865 298 4581
Fragments arranged by size:
4581 950 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3010 3080 3689 5357 882
Size: 617 1511 70 609 1668 2219
Fragments arranged by size:
2219 1668 1511 617 609 70
BsaAI yAC'GTr
Cuts at: 493 1204 4485 493
Size: 711 3281 2702
Fragments arranged by size:
3281 2702 711
BsaBI GATnn'nnATC
Cuts at: 6688 6688
Size: 6694
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3503 4501 4883 5518
Size: 53 83 186 936 1970 998 382 635
Cuts at: 5518 275
Size: 1451
Fragments arranged by size:
1970 1451 998 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2538 5127 5525 5799 5946 6357 2538
Size: 2589 398 274 147 411 2875
Fragments arranged by size:
2875 2589 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3301 3331 3787 3817 1178
Size: 30 165 30 1898 30 456 30 4055
Fragments arranged by size:
4055 1898 456 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 3134 3625 3795 3847 1186
Size: 1309 639 491 170 52 4033
Fragments arranged by size:
4033 1309 639 491 170 52
BseYI C'CCAG_C
Cuts at: 2101 2315 2582 2700 3206 3476 3539 3809 3934
Size: 214 267 118 506 270 63 270 125
Cuts at: 3934 5897 6393 2101
Size: 1963 496 2402
Fragments arranged by size:
2402 1963 506 496 270 270 267 214
125 118 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2364 1163
Size: 573 628 5493
Fragments arranged by size:
5493 628 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4144 4905 5933 1540
Size: 275 186 2143 761 1028 2301
Fragments arranged by size:
2301 2143 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3855 4755 4840 5911 3711
Size: 144 900 85 1071 4494
Fragments arranged by size:
4494 1071 900 144 85
BsmI GAATG_Cn'
Cuts at: 2470 4205 4298 2470
Size: 1735 93 4866
Fragments arranged by size:
4866 1735 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3154 3154
Size: 6694
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 2809 3207 3248 3442 3540 3667
Size: 399 637 382 398 41 194 98 127
Cuts at: 3667 3711 3855 3984 4013 4755 4840 5911 1391
Size: 44 144 129 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 637 399 398 382 194
144 129 127 98 85 44 41 29
BspEI T'CCGG_A
Cuts at: 2538 6357 2538
Size: 3819 2875
BspHI T'CATG_A
Cuts at: 4583 6313 4583
Size: 1730 4964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2110 4049 844
Size: 1266 1939 3489
Fragments arranged by size:
3489 1939 1266
BsrBI CCG'CTC
Cuts at: 1394 1823 4581 1394
Size: 429 2758 3507
Fragments arranged by size:
3507 2758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3564 5189 5363 65
Size: 1183 2316 1625 174 1396
Fragments arranged by size:
2316 1625 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5338 5525 1700
Size: 110 3528 187 2869
Fragments arranged by size:
3528 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6694
BssSI C'ACGA_G
Cuts at: 4754 5766 4754
Size: 1012 5682
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6694
BstBI TT'CG_AA
Cuts at: 5498 5498
Size: 6694
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6694
BstXI CCAn_nnnn'nTGG
Cuts at: 2893 2893
Size: 6694
BstYI r'GATC_y
Cuts at: 1972 2223 2340 3014 3123 3923 4024 4391 4776
Size: 251 117 674 109 800 101 367 385
Cuts at: 4776 4793 6245 6331 6343 6689 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 800 674 385 367 346 251
117 109 101 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2946 3701 3859 2946
Size: 755 158 5781
Fragments arranged by size:
5781 755 158
BtgI C'CryG_G
Cuts at: 513 1065 2004 2286 2955 3234 3279 3510 3669
Size: 552 939 282 669 279 45 231 159
Cuts at: 3669 513
Size: 3538
Fragments arranged by size:
3538 939 669 552 282 279 231 159
45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5955
Fragments arranged by size:
5955 683 56
BtsI GCAGTG_nn'
Cuts at: 4210 5004 5024 4210
Size: 794 20 5880
Fragments arranged by size:
5880 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3732 3767 529
Size: 35 3168 35 3456
Fragments arranged by size:
3456 3168 35 35
DraI TTT'AAA
Cuts at: 3998 3998
Size: 6694
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2897 5701 809
Size: 507 1581 2804 1802
Fragments arranged by size:
2804 1802 1581 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2838 4141 5030
Size: 54 1425 50 275 18 1008 1303 889
Cuts at: 5030 6659 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1303 1008 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4141 1537
Size: 275 2329 4090
Fragments arranged by size:
4090 2329 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2525 2661 3046 3687 4624 1353
Size: 1172 136 385 641 937 3423
Fragments arranged by size:
3423 1172 937 641 385 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5281 5655 5801 387
Size: 1064 138 3692 374 146 1280
Fragments arranged by size:
3692 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3709 3853 3709
Size: 144 6550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2329 2476 2498 2983 3062 3138
Size: 393 548 149 147 22 485 79 76
Cuts at: 3138 3183 3320 3711 3716 4757 5354 6141 6409
Size: 45 137 391 5 1041 597 787 268
Cuts at: 6409 1239
Size: 1524
Fragments arranged by size:
1524 1041 787 597 548 485 393 391
268 149 147 137 79 76 45 22
5
EcoNI CCTnn'n_nnAGG
Cuts at: 3577 3577
Size: 6694
EcoO109I rG'GnC_Cy
Cuts at: 2199 2890 3536 3630 3663 3664 3870 2199
Size: 691 646 94 33 1 206 5023
Fragments arranged by size:
5023 691 646 206 94 33 1
EcoRI G'AATT_C
Cuts at: 4003 4381 4003
Size: 378 6316
EcoRV GAT'ATC
Cuts at: 2071 2323 2071
Size: 252 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 3388 2359
Size: 1029 5665
FspI TGC'GCA
Cuts at: 5200 5200
Size: 6694
HaeII r_GCGC'y
Cuts at: 1678 3506 5841 1678
Size: 1828 2335 2531
Fragments arranged by size:
2531 2335 1828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2203 2235 3787 3819 5335
Size: 32 135 32 631 32 1552 32 1516
Cuts at: 5335 5367 5409 5441 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1552 1516 631 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2283 4038 4284 669
Size: 988 281 345 1755 246 3079
Fragments arranged by size:
3079 1755 988 345 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6694
HpaI GTT'AAC
Cuts at: 4284 4284
Size: 6694
KasI G'GCGC_C
Cuts at: 3502 3502
Size: 6694
KpnI G_GTAC'C
Cuts at: 2126 4019 2126
Size: 1893 4801
MfeI C'AATT_G
Cuts at: 4293 4293
Size: 6694
MluI A'CGCG_T
Cuts at: 6570 6570
Size: 6694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 3515 3767 5808 5992 1305
Size: 840 1370 252 2041 184 2007
Fragments arranged by size:
2041 2007 1370 840 252 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2840 10
Size: 54 1425 343 1008 3864
Fragments arranged by size:
3864 1425 1008 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3974 4652 5011 5170 518
Size: 648 2808 678 359 159 2042
Fragments arranged by size:
2808 2042 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2105 2288 2387 3350 4110 4787 5935
Size: 474 25 183 99 963 760 677 1148
Cuts at: 5935 6180 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 963 760 677 474 245 183
99 25
NarI GG'CG_CC
Cuts at: 3503 3503
Size: 6694
NcoI C'CATG_G
Cuts at: 513 1065 2004 3669 513
Size: 552 939 1665 3538
Fragments arranged by size:
3538 1665 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6694
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6694
NotI GC'GGCC_GC
Cuts at: 4141 4141
Size: 6694
NspI r_CATG'y
Cuts at: 1132 1229 3071 4052 5597 1132
Size: 97 1842 981 1545 2229
Fragments arranged by size:
2229 1842 1545 981 97
PasI CC'CwG_GG
Cuts at: 1348 3025 3434 3554 1348
Size: 1677 409 120 4488
Fragments arranged by size:
4488 1677 409 120
PciI A'CATG_T
Cuts at: 5593 5593
Size: 6694
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 2754 3472 1108
Size: 972 674 718 4330
Fragments arranged by size:
4330 972 718 674
PfoI T'CCnGG_A
Cuts at: 2226 2325 2226
Size: 99 6595
PmeI GTTT'AAAC
Cuts at: 3998 3998
Size: 6694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3837 3869 4763 4795 6276 6308 3837
Size: 32 894 32 1481 32 4223
Fragments arranged by size:
4223 1481 894 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2199 2890 3630 3870 2199
Size: 691 740 240 5023
Fragments arranged by size:
5023 740 691 240
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6694
PsiI TTA'TAA
Cuts at: 4264 4264
Size: 6694
PspOMI G'GGCC_C
Cuts at: 3536 3663 3536
Size: 127 6567
PstI C_TGCA'G
Cuts at: 830 3264 4046 830
Size: 2434 782 3478
Fragments arranged by size:
3478 2434 782
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6694
PvuII CAG'CTG
Cuts at: 2080 2105 2387 2080
Size: 25 282 6387
Fragments arranged by size:
6387 282 25
SacI G_AGCT'C
Cuts at: 3711 3855 3711
Size: 144 6550
SacII CC_GC'GG
Cuts at: 2289 2289
Size: 6694
SalI G'TCGA_C
Cuts at: 1936 4036 1936
Size: 2100 4594
SanDI GG'GwC_CC
Cuts at: 3870 3870
Size: 6694
SapI GCTCTTCn'nnn_
Cuts at: 2661 3046 2661
Size: 385 6309
SbfI CC_TGCA'GG
Cuts at: 4046 4046
Size: 6694
ScaI AGT'ACT
Cuts at: 3310 4942 6654 3310
Size: 1632 1712 3350
Fragments arranged by size:
3350 1712 1632
SexAI A'CCwGG_T
Cuts at: 2892 2892
Size: 6694
SfcI C'TryA_G
Cuts at: 826 1045 2018 3260 3594 4042 5177 5858 6049
Size: 219 973 1242 334 448 1135 681 191
Cuts at: 6049 826
Size: 1471
Fragments arranged by size:
1471 1242 1135 973 681 448 334 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2957 2966 2957
Size: 9 6685
SfoI GGC'GCC
Cuts at: 3504 3504
Size: 6694
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6694
SmaI CCC'GGG
Cuts at: 1872 4021 1872
Size: 2149 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3848 4798 5699 5961 820
Size: 197 926 1905 950 901 262 1553
Fragments arranged by size:
1905 1553 950 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4485 493
Size: 3992 2702
SpeI A'CTAG_T
Cuts at: 152 2453 152
Size: 2301 4393
SphI G_CATG'C
Cuts at: 1132 4052 1132
Size: 2920 3774
SspI AAT'ATT
Cuts at: 5 52 4618 5
Size: 47 4566 2081
Fragments arranged by size:
4566 2081 47
StuI AGG'CCT
Cuts at: 1517 2489 2951 3386 1517
Size: 972 462 435 4825
Fragments arranged by size:
4825 972 462 435
StyI C'CwwG_G
Cuts at: 513 1065 2004 2547 2886 3220 3605 3669 4133
Size: 552 939 543 339 334 385 64 464
Cuts at: 4133 513
Size: 3074
Fragments arranged by size:
3074 939 552 543 464 385 339 334
64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3062 3523 4721 4738 4891 5076 5521
Size: 1255 1482 461 1198 17 153 185 445
Cuts at: 5521 325
Size: 1498
Fragments arranged by size:
1498 1482 1255 1198 461 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2685 3308
Size: 274 80 33 51 1443 171 537 623
Cuts at: 3308 4940 6652 96
Size: 1632 1712 138
Fragments arranged by size:
1712 1632 1443 623 537 274 171 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6654 6686 6654
Size: 32 6662
Tth111I GACn'n_nGTC
Cuts at: 1316 3799 1316
Size: 2483 4211
XbaI T'CTAG_A
Cuts at: 4030 4030
Size: 6694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2893 2962 3473 2323
Size: 570 69 511 5544
Fragments arranged by size:
5544 570 511 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6694
XmaI C'CCGG_G
Cuts at: 1870 4019 1870
Size: 2149 4545
XmnI GAAnn'nnTTC
Cuts at: 2361 4823 2361
Size: 2462 4232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4502 276
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbvCI BfrBI BsiWI BstZ17I
DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SrfI SwaI