Restriction map for
pFN21AB6194
(Circular) (Six-base) MAPSORT of: pFN21AB6194.seq Check: 1481 from: 1 to: 6823
pFN21AB6194 6823 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2712 3507 4166 1937
Size: 775 795 659 4594
Fragments arranged by size:
4594 795 775 659
Acc65I G'GTAC_C
Cuts at: 4144 4144
Size: 6823
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2892 2920 3539 4886 6270 6538 1632
Size: 1260 28 619 1347 1384 268 1917
Fragments arranged by size:
1917 1384 1347 1260 619 268 28
AfeI AGC'GCT
Cuts at: 2877 2877
Size: 6823
AflII C'TTAA_G
Cuts at: 820 1017 2914 820
Size: 197 1897 4729
Fragments arranged by size:
4729 1897 197
AflIII A'CryG_T
Cuts at: 1203 3333 3591 5722 6699 1203
Size: 2130 258 2131 977 1327
Fragments arranged by size:
2131 2130 1327 977 258
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 2047 3691 5552 2047
Size: 1644 1861 3318
Fragments arranged by size:
3318 1861 1644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2825 1184
Size: 1641 5182
AlwNI CAG_nnn'CTG
Cuts at: 3089 4566 6138 3089
Size: 1477 1572 3774
Fragments arranged by size:
3774 1572 1477
ApaLI G'TGCA_C
Cuts at: 2629 3274 4880 6036 2629
Size: 645 1606 1156 3416
Fragments arranged by size:
3416 1606 1156 645
ApoI r'AATT_y
Cuts at: 1493 2402 3197 4004 4132 4358 4510 6619 1493
Size: 909 795 807 128 226 152 2109 1697
Fragments arranged by size:
2109 1697 909 807 795 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 5377 160
Size: 872 4345 1606
Fragments arranged by size:
4345 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 4100 4148 1870
Size: 73 2157 48 4545
Fragments arranged by size:
4545 2157 73 48
BamHI G'GATC_C
Cuts at: 4153 4520 4153
Size: 367 6456
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2444 4144 5599 618
Size: 325 217 627 657 1700 1455 1842
Fragments arranged by size:
1842 1700 1455 657 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2271 2729 3765 4142 1391
Size: 880 458 1036 377 4072
Fragments arranged by size:
4072 1036 880 458 377
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 3178 928
Size: 971 416 863 4573
Fragments arranged by size:
4573 971 863 416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2474 2508 3976 4010 5014 5048 1903
Size: 34 537 34 1468 34 1004 34 3678
Fragments arranged by size:
3678 1468 1004 537 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4715 5925 4715
Size: 1210 5613
BclI T'GATC_A
Cuts at: 1548 4050 1548
Size: 2502 4321
BfrBI ATG'CAT
Cuts at: 2795 2795
Size: 6823
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5434 136
Size: 107 122 71 631 605 3762 1525
Fragments arranged by size:
3762 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2931 3651 6818 2931
Size: 720 3167 2936
Fragments arranged by size:
3167 2936 720
BlpI GC'TnA_GC
Cuts at: 4240 4240
Size: 6823
Bme1580I G_kGCm'C
Cuts at: 1790 2633 3278 4884 6040 1790
Size: 843 645 1606 1156 2573
Fragments arranged by size:
2573 1606 1156 843 645
BmgBI CAC'GTC
Cuts at: 1318 2473 1318
Size: 1155 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3928 4554 5512
Size: 416 53 83 399 24 2511 626 958
Cuts at: 5512 442
Size: 1753
Fragments arranged by size:
2511 1753 958 626 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 6823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3830 5483 1239
Size: 2591 1653 2579
Fragments arranged by size:
2591 2579 1653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2322 2768 4948 5813 6111 2322
Size: 446 2180 865 298 3034
Fragments arranged by size:
3034 2180 865 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2966 5486 882
Size: 617 1467 2520 2219
Fragments arranged by size:
2520 2219 1467 617
BsaAI yAC'GTr
Cuts at: 493 1204 4614 493
Size: 711 3410 2702
Fragments arranged by size:
3410 2702 711
BsaBI GATnn'nnATC
Cuts at: 2138 6817 2138
Size: 4679 2144
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4630 5012 5647 275
Size: 53 83 186 936 3097 382 635 1451
Fragments arranged by size:
3097 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2947 5256 5654 5928 6075 6486 2947
Size: 2309 398 274 147 411 3284
Fragments arranged by size:
3284 2309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3035 3065 3305 3335 3578
Size: 30 165 30 1632 30 240 30 243
Cuts at: 3578 3608 1178
Size: 30 4393
Fragments arranged by size:
4393 1632 243 240 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3770 3773 1186
Size: 2584 3 4236
Fragments arranged by size:
4236 2584 3
BseYI C'CCAG_C
Cuts at: 3658 3868 6026 6522 3658
Size: 210 2158 496 3959
Fragments arranged by size:
3959 2158 496 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2311 1163
Size: 573 575 5675
Fragments arranged by size:
5675 575 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3962 4273 5034 6062 1540
Size: 275 186 1961 311 761 1028 2301
Fragments arranged by size:
2301 1961 1028 761 311 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2271 2633 2897 3278 3765 4048 4884 4969
Size: 171 362 264 381 487 283 836 85
Cuts at: 4969 6040 2100
Size: 1071 2883
Fragments arranged by size:
2883 1071 836 487 381 362 283 264
171 85
BsmI GAATG_Cn'
Cuts at: 2352 2362 4334 4427 2352
Size: 10 1972 93 4748
Fragments arranged by size:
4748 1972 93 10
BsmBI CGTCTCn'nnnn_
Cuts at: 2402 2402
Size: 6823
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2271 2633 2729 2897 3278 3765
Size: 399 310 171 362 96 168 381 487
Cuts at: 3765 4048 4142 4884 4969 6040 1391
Size: 283 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 399 381 362 310
283 171 168 96 94 85
BspEI T'CCGG_A
Cuts at: 6486 6486
Size: 6823
BspHI T'CATG_A
Cuts at: 4092 4712 6442 4092
Size: 620 1730 4473
Fragments arranged by size:
4473 1730 620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4178 844
Size: 3334 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4111 4710 1394
Size: 429 2288 599 3507
Fragments arranged by size:
3507 2288 599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2590 5318 5492 65
Size: 1183 1342 2728 174 1396
Fragments arranged by size:
2728 1396 1342 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5467 5654 1700
Size: 110 3657 187 2869
Fragments arranged by size:
3657 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3341 96
Size: 3245 3578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 2483 2534 4883 5895 2483
Size: 51 2349 1012 3411
Fragments arranged by size:
3411 2349 1012 51
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6823
BstBI TT'CG_AA
Cuts at: 5627 5627
Size: 6823
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6823
BstXI CCAn_nnnn'nTGG
Cuts at: 3781 3781
Size: 6823
BstYI r'GATC_y
Cuts at: 1972 2931 3651 4153 4520 4905 4922 6374 6460
Size: 959 720 502 367 385 17 1452 86
Cuts at: 6460 6472 6818 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 959 720 502 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3027 3027
Size: 6823
BtgI C'CryG_G
Cuts at: 513 1065 2262 3742 3800 513
Size: 552 1197 1480 58 3536
Fragments arranged by size:
3536 1480 1197 552 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3625 487
Size: 683 56 2399 3685
Fragments arranged by size:
3685 2399 683 56
BtsI GCAGTG_nn'
Cuts at: 2490 4339 5133 5153 2490
Size: 1849 794 20 4160
Fragments arranged by size:
4160 1849 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2827 2862 529
Size: 35 2263 35 4490
Fragments arranged by size:
4490 2263 35 35
DraI TTT'AAA
Cuts at: 2707 4127 2707
Size: 1420 5403
DraIII CAC_nnn'GTG
Cuts at: 3682 3682
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5830 809
Size: 507 4514 1802
Fragments arranged by size:
4514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2835 2880 3959
Size: 54 1425 50 275 18 1005 45 1079
Cuts at: 3959 4270 5159 6788 8
Size: 311 889 1629 43
Fragments arranged by size:
1629 1425 1079 1005 889 311 275 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3959 4270 1537
Size: 275 2147 311 4090
Fragments arranged by size:
4090 2147 311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2200 3284 3770 3848 3857 4753 1353
Size: 847 1084 486 78 9 896 3423
Fragments arranged by size:
3423 1084 896 847 486 78 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3878 5410 5784 5930 387
Size: 1064 138 2289 1532 374 146 1280
Fragments arranged by size:
2289 1532 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2269 3763 2269
Size: 1494 5329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2892 2920 3539 3830 4886 5483 6270
Size: 393 1260 28 619 291 1056 597 787
Cuts at: 6270 6538 1239
Size: 268 1524
Fragments arranged by size:
1524 1260 1056 787 619 597 393 291
268 28
EcoO109I rG'GnC_Cy
Cuts at: 2049 2548 2751 3514 3826 2049
Size: 499 203 763 312 5046
Fragments arranged by size:
5046 763 499 312 203
EcoRI G'AATT_C
Cuts at: 3197 4004 4132 4510 3197
Size: 807 128 378 5510
Fragments arranged by size:
5510 807 378 128
EcoRV GAT'ATC
Cuts at: 4119 4119
Size: 6823
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 2440 2879 5970 1678
Size: 762 439 3091 2531
Fragments arranged by size:
3091 2531 762 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2234 2266 2628
Size: 32 135 32 461 32 169 32 362
Cuts at: 2628 2660 2694 2726 3677 3709 4035 4067 5464
Size: 32 34 32 951 32 326 32 1397
Cuts at: 5464 5496 5538 5570 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1397 951 461 362 326 169 135
42 34 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2080 4167 4413 669
Size: 988 281 142 2087 246 3079
Fragments arranged by size:
3079 2087 988 281 246 142
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 2080 4413 2080
Size: 2333 4490
KpnI G_GTAC'C
Cuts at: 4148 4148
Size: 6823
MfeI C'AATT_G
Cuts at: 4422 4422
Size: 6823
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 3033 5937 6121 1305
Size: 753 975 2904 184 2007
Fragments arranged by size:
2904 2007 975 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2837 2882 10
Size: 54 1425 343 1005 45 3951
Fragments arranged by size:
3951 1425 1005 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 3779 4781 5140 5299 518
Size: 648 1641 972 1002 359 159 2042
Fragments arranged by size:
2042 1641 1002 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4239 4916 6064 6309 1606
Size: 2633 677 1148 245 2120
Fragments arranged by size:
2633 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6271
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6823
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6823
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NsiI A_TGCA'T
Cuts at: 2797 2797
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 2695 2799 3337 3595 4181 5726 1132
Size: 97 1466 104 538 258 586 1545 2229
Fragments arranged by size:
2229 1545 1466 586 538 258 104 97
PasI CC'CwG_GG
Cuts at: 1348 2169 1348
Size: 821 6002
PciI A'CATG_T
Cuts at: 3333 3591 5722 3333
Size: 258 2131 4434
Fragments arranged by size:
4434 2131 258
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3682 1108
Size: 2574 4249
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4924 6405 6437 4892
Size: 32 1481 32 5278
Fragments arranged by size:
5278 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2049 2751 3514 3826 2049
Size: 702 763 312 5046
Fragments arranged by size:
5046 763 702 312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 4393 4393
Size: 6823
PstI C_TGCA'G
Cuts at: 830 3120 3483 3989 4175 830
Size: 2290 363 506 186 3478
Fragments arranged by size:
3478 2290 506 363 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
SacI G_AGCT'C
Cuts at: 2271 3765 2271
Size: 1494 5329
SalI G'TCGA_C
Cuts at: 1936 4165 1936
Size: 2229 4594
SanDI GG'GwC_CC
Cuts at: 2751 2751
Size: 6823
SapI GCTCTTCn'nnn_
Cuts at: 3770 3848 3770
Size: 78 6745
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 5071 6783 5071
Size: 1712 5111
SfcI C'TryA_G
Cuts at: 826 1045 3116 3479 3508 3985 4171 5306 5987
Size: 219 2071 363 29 477 186 1135 681
Cuts at: 5987 6178 826
Size: 191 1471
Fragments arranged by size:
2071 1471 1135 681 477 363 219 191
186 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 4102 4150 1872
Size: 2230 48 4545
Fragments arranged by size:
4545 2230 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2337 2783 2914 4927 5828 6090
Size: 197 926 394 446 131 2013 901 262
Cuts at: 6090 820
Size: 1553
Fragments arranged by size:
2013 1553 926 901 446 394 262 197
131
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 4181 1132
Size: 3049 3774
SspI AAT'ATT
Cuts at: 5 52 4747 5
Size: 47 4695 2081
Fragments arranged by size:
4695 2081 47
StuI AGG'CCT
Cuts at: 1517 3233 1517
Size: 1716 5107
StyI C'CwwG_G
Cuts at: 513 1065 3303 3354 4262 513
Size: 552 2238 51 908 3074
Fragments arranged by size:
3074 2238 908 552 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4850 4867 5020 5205 5650 325
Size: 1255 3270 17 153 185 445 1498
Fragments arranged by size:
3270 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3341 5069 6781
Size: 274 80 33 51 1443 1364 1728 1712
Cuts at: 6781 96
Size: 138
Fragments arranged by size:
1728 1712 1443 1364 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 6783 6815 3056
Size: 32 3695 32 3064
Fragments arranged by size:
3695 3064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6823
XbaI T'CTAG_A
Cuts at: 4159 4159
Size: 6823
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 4100 4148 1870
Size: 2230 48 4545
Fragments arranged by size:
4545 2230 48
XmnI GAAnn'nnTTC
Cuts at: 3468 3617 3842 4073 4952 3468
Size: 149 225 231 879 5339
Fragments arranged by size:
5339 879 231 225 149
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI
SrfI SwaI XcmI