Restriction map for
pFN21AB6190
(Circular) (Six-base) MAPSORT of: pFN21AB6190.seq Check: 6785 from: 1 to: 6682
pFN21AB6190 6682 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4492 278
Size: 53 83 186 936 2956 2468
Fragments arranged by size:
2956 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4025 1937
Size: 2088 4594
Acc65I G'GTAC_C
Cuts at: 4003 4003
Size: 6682
AclI AA'CG_TT
Cuts at: 1563 4809 5182 1563
Size: 3246 373 3063
Fragments arranged by size:
3246 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2422 2740 2935 3439 3523 4745 6129 6397
Size: 790 318 195 504 84 1222 1384 268
Cuts at: 6397 1632
Size: 1917
Fragments arranged by size:
1917 1384 1222 790 504 318 268 195
84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6485
AflIII A'CryG_T
Cuts at: 1203 2357 2487 3345 5581 6558 1203
Size: 1154 130 858 2236 977 1327
Fragments arranged by size:
2236 1327 1154 977 858 130
AgeI A'CCGG_T
Cuts at: 5513 5513
Size: 6682
AhdI GACnn_n'nnGTC
Cuts at: 5411 5411
Size: 6682
AleI CACnn'nnGTG
Cuts at: 1166 2274 3473 1166
Size: 1108 1199 4375
Fragments arranged by size:
4375 1199 1108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6682
AlwNI CAG_nnn'CTG
Cuts at: 2350 4425 5997 2350
Size: 2075 1572 3035
Fragments arranged by size:
3035 2075 1572
ApaLI G'TGCA_C
Cuts at: 2353 4739 5895 2353
Size: 2386 1156 3140
Fragments arranged by size:
3140 2386 1156
ApoI r'AATT_y
Cuts at: 1493 2900 3069 3197 3237 3281 3533 3573 3785
Size: 1407 169 128 40 44 252 40 212
Cuts at: 3785 3991 4217 4369 6478 1493
Size: 206 226 152 2109 1697
Fragments arranged by size:
2109 1697 1407 252 226 212 206 169
152 128 44 40 40
AseI AT'TA_AT
Cuts at: 160 1032 2805 5236 160
Size: 872 1773 2431 1606
Fragments arranged by size:
2431 1773 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 4007 1870
Size: 73 133 1931 4545
Fragments arranged by size:
4545 1931 133 73
BamHI G'GATC_C
Cuts at: 4012 4379 4012
Size: 367 6315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 4003 5458 618
Size: 325 217 627 404 1812 1455 1842
Fragments arranged by size:
1842 1812 1455 627 404 325 217
BanII G_rGCy'C
Cuts at: 1391 3931 4001 1391
Size: 2540 70 4072
Fragments arranged by size:
4072 2540 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5711
BbvCI CC'TCA_GC
Cuts at: 2742 2742
Size: 6682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4873 4907 1903
Size: 34 2936 34 3678
Fragments arranged by size:
3678 2936 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4574 5784 4574
Size: 1210 5472
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6682
BfrBI ATG'CAT
Cuts at: 2604 3267 2604
Size: 663 6019
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5293 136
Size: 107 122 71 631 605 3621 1525
Fragments arranged by size:
3621 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3002 6677 3002
Size: 3675 3007
BlpI GC'TnA_GC
Cuts at: 4099 4099
Size: 6682
Bme1580I G_kGCm'C
Cuts at: 1790 2357 4743 5899 1790
Size: 567 2386 1156 2573
Fragments arranged by size:
2573 2386 1156 567
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6682
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4413 5371 442
Size: 416 53 83 399 24 2996 958 1753
Fragments arranged by size:
2996 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2611 3015 3183 3253 3435 3519 3603
Size: 917 455 404 168 70 182 84 84
Cuts at: 3603 3619 3771 3855 3939 5342 1239
Size: 16 152 84 84 1403 2579
Fragments arranged by size:
2579 1403 917 455 404 182 168 152
84 84 84 84 70 16
Bpu10I CC'TnA_GC
Cuts at: 2742 2742
Size: 6682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 4807 5672 5970 2117
Size: 2690 865 298 2829
Fragments arranged by size:
2829 2690 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2743 5345 882
Size: 617 1244 2602 2219
Fragments arranged by size:
2602 2219 1244 617
BsaAI yAC'GTr
Cuts at: 493 1204 2360 2508 3346 3850 4473 493
Size: 711 1156 148 838 504 623 2702
Fragments arranged by size:
2702 1156 838 711 623 504 148
BsaBI GATnn'nnATC
Cuts at: 6676 6676
Size: 6682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4489 4871 5506 275
Size: 53 83 186 936 2956 382 635 1451
Fragments arranged by size:
2956 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5115 5513 5787 5934 6345 5115
Size: 398 274 147 411 5452
Fragments arranged by size:
5452 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6457
Fragments arranged by size:
6457 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2114 1186
Size: 910 18 5754
Fragments arranged by size:
5754 910 18
BseYI C'CCAG_C
Cuts at: 5885 6381 5885
Size: 496 6186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2530 3202 3370 3454 3706 3790 3800
Size: 573 794 672 168 84 252 84 10
Cuts at: 3800 1163
Size: 4045
Fragments arranged by size:
4045 794 672 573 252 168 84 84
10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4132 4893 5921 1540
Size: 275 186 2131 761 1028 2301
Fragments arranged by size:
2301 2131 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2357 3231 3931 4743 4828 5899 2357
Size: 874 700 812 85 1071 3140
Fragments arranged by size:
3140 1071 874 812 700 85
BsmI GAATG_Cn'
Cuts at: 2606 3269 4193 4286 2606
Size: 663 924 93 5002
Fragments arranged by size:
5002 924 663 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2357 3231 3931 4001 4743 4828 5899
Size: 399 567 874 700 70 742 85 1071
Cuts at: 5899 1391
Size: 2174
Fragments arranged by size:
2174 1071 874 742 700 567 399 85
70
BspEI T'CCGG_A
Cuts at: 6345 6345
Size: 6682
BspHI T'CATG_A
Cuts at: 4571 6301 4571
Size: 1730 4952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4037 844
Size: 3193 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4569 1394
Size: 429 2746 3507
Fragments arranged by size:
3507 2746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3610 5177 5351 65
Size: 1183 2362 1567 174 1396
Fragments arranged by size:
2362 1567 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5326 5513 1700
Size: 110 3516 187 2869
Fragments arranged by size:
3516 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2477 3562 96
Size: 2381 1085 3216
Fragments arranged by size:
3216 2381 1085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6682
BssSI C'ACGA_G
Cuts at: 3932 4742 5754 3932
Size: 810 1012 4860
Fragments arranged by size:
4860 1012 810
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6682
BstBI TT'CG_AA
Cuts at: 5486 5486
Size: 6682
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6682
BstYI r'GATC_y
Cuts at: 1972 2498 3002 3338 4012 4379 4764 4781 6233
Size: 526 504 336 674 367 385 17 1452
Cuts at: 6233 6319 6331 6677 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 674 526 504 385 367 346
336 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5191
Fragments arranged by size:
5191 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5943
Fragments arranged by size:
5943 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3022 3190 3442 3694 3778 4198 4992 5012
Size: 84 168 252 252 84 420 794 20
Cuts at: 5012 2938
Size: 4608
Fragments arranged by size:
4608 794 420 252 252 168 84 84
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3088 3123 3592 3627 529
Size: 35 2524 35 469 35 3584
Fragments arranged by size:
3584 2524 469 35 35 35
DraI TTT'AAA
Cuts at: 3986 3986
Size: 6682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2245 5689 809
Size: 507 929 3444 1802
Fragments arranged by size:
3444 1802 929 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4129 5018 6647
Size: 54 1425 50 275 18 2299 889 1629
Cuts at: 6647 8
Size: 43
Fragments arranged by size:
2299 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4129 1537
Size: 275 2317 4090
Fragments arranged by size:
4090 2317 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2465 2721 4612 1353
Size: 1112 256 1891 3423
Fragments arranged by size:
3423 1891 1112 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5269 5643 5789 387
Size: 1064 138 3680 374 146 1280
Fragments arranged by size:
3680 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3929 3929
Size: 6682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2422 2611 2740 2935 3015 3183
Size: 393 524 266 189 129 195 80 168
Cuts at: 3183 3253 3435 3439 3519 3523 3603 3619 3771
Size: 70 182 4 80 4 80 16 152
Cuts at: 3771 3855 3939 4745 5342 6129 6397 1239
Size: 84 84 806 597 787 268 1524
Fragments arranged by size:
1524 806 787 597 524 393 268 266
195 189 182 168 152 129 84 84
80 80 80 70 16 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2099
Size: 6682
EcoO109I rG'GnC_Cy
Cuts at: 2457 3255 2457
Size: 798 5884
EcoRI G'AATT_C
Cuts at: 3069 3237 3573 3991 4369 3069
Size: 168 336 418 378 5382
Fragments arranged by size:
5382 418 378 336 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3152 3404 3488 3152
Size: 252 84 6346
Fragments arranged by size:
6346 252 84
FspI TGC'GCA
Cuts at: 5188 5188
Size: 6682
HaeII r_GCGC'y
Cuts at: 1678 5829 1678
Size: 4151 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5323 5355 5397 5429 1373
Size: 32 135 32 3751 32 42 32 2626
Fragments arranged by size:
3751 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4026 4272 669
Size: 988 281 2088 246 3079
Fragments arranged by size:
3079 2088 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6682
HpaI GTT'AAC
Cuts at: 4272 4272
Size: 6682
KpnI G_GTAC'C
Cuts at: 4007 4007
Size: 6682
MfeI C'AATT_G
Cuts at: 4281 4281
Size: 6682
MluI A'CGCG_T
Cuts at: 6558 6558
Size: 6682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3576 5796 5980 1305
Size: 2271 2220 184 2007
Fragments arranged by size:
2271 2220 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4860
Fragments arranged by size:
4860 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2272 2274 3473 4640 4999 5158 518
Size: 648 1106 2 1199 1167 359 159 2042
Fragments arranged by size:
2042 1199 1167 1106 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2012 4098 4775 5923 6168 1606
Size: 406 2086 677 1148 245 2120
Fragments arranged by size:
2120 2086 1148 677 406 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5191
Fragments arranged by size:
5191 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6682
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6682
NotI GC'GGCC_GC
Cuts at: 4129 4129
Size: 6682
NsiI A_TGCA'T
Cuts at: 2606 3269 2606
Size: 663 6019
NspI r_CATG'y
Cuts at: 1132 1229 2491 2931 4040 5585 1132
Size: 97 1262 440 1109 1545 2229
Fragments arranged by size:
2229 1545 1262 1109 440 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6682
PciI A'CATG_T
Cuts at: 2487 5581 2487
Size: 3094 3588
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6682
PfoI T'CCnGG_A
Cuts at: 2700 2700
Size: 6682
PmeI GTTT'AAAC
Cuts at: 3986 3986
Size: 6682
PmlI CAC'GTG
Cuts at: 2360 2360
Size: 6682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2045 2077 4751 4783 6264 6296 2045
Size: 32 2674 32 1481 32 2431
Fragments arranged by size:
2674 2431 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6682
PsiI TTA'TAA
Cuts at: 3093 4252 3093
Size: 1159 5523
PstI C_TGCA'G
Cuts at: 830 3017 4034 830
Size: 2187 1017 3478
Fragments arranged by size:
3478 2187 1017
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6682
SacI G_AGCT'C
Cuts at: 3931 3931
Size: 6682
SalI G'TCGA_C
Cuts at: 1936 4024 1936
Size: 2088 4594
SapI GCTCTTCn'nnn_
Cuts at: 2465 2465
Size: 6682
SbfI CC_TGCA'GG
Cuts at: 4034 4034
Size: 6682
ScaI AGT'ACT
Cuts at: 4930 6642 4930
Size: 1712 4970
SfcI C'TryA_G
Cuts at: 826 1045 2214 3013 3034 3874 4030 5165 5846
Size: 219 1169 799 21 840 156 1135 681
Cuts at: 5846 6037 826
Size: 191 1471
Fragments arranged by size:
1471 1169 1135 840 799 681 219 191
156 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6682
SmaI CCC'GGG
Cuts at: 1872 4009 1872
Size: 2137 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 4786 5687 5949 820
Size: 197 926 153 2690 901 262 1553
Fragments arranged by size:
2690 1553 926 901 262 197 153
SnaBI TAC'GTA
Cuts at: 493 3850 4473 493
Size: 3357 623 2702
Fragments arranged by size:
3357 2702 623
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6682
SphI G_CATG'C
Cuts at: 1132 4040 1132
Size: 2908 3774
SspI AAT'ATT
Cuts at: 5 52 4606 5
Size: 47 4554 2081
Fragments arranged by size:
4554 2081 47
StuI AGG'CCT
Cuts at: 1517 2948 3088 3172 3368 3424 3452 3508 3676
Size: 1431 140 84 196 56 28 56 168
Cuts at: 3676 1517
Size: 4523
Fragments arranged by size:
4523 1431 196 168 140 84 56 56
28
StyI C'CwwG_G
Cuts at: 513 1065 2004 2644 4121 513
Size: 552 939 640 1477 3074
Fragments arranged by size:
3074 1477 939 640 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2286 4709 4726 4879 5064 5509 325
Size: 1255 706 2423 17 153 185 445 1498
Fragments arranged by size:
2423 1498 1255 706 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2477 3562
Size: 274 80 33 51 1443 90 410 1085
Cuts at: 3562 4928 6640 96
Size: 1366 1712 138
Fragments arranged by size:
1712 1443 1366 1085 410 274 138 90
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 6642 6674 2097
Size: 32 4513 32 2105
Fragments arranged by size:
4513 2105 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6682
XbaI T'CTAG_A
Cuts at: 4018 4018
Size: 6682
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6682
XmaI C'CCGG_G
Cuts at: 1870 4007 1870
Size: 2137 4545
XmnI GAAnn'nnTTC
Cuts at: 2435 2696 2948 3452 4811 2435
Size: 261 252 504 1359 4306
Fragments arranged by size:
4306 1359 504 261 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4490 276
Size: 53 83 186 936 2956 2468
Fragments arranged by size:
2956 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI