Restriction map for
pFN21AB6189
(Circular) (Six-base) MAPSORT of: pFN21AB6189.seq Check: 8584 from: 1 to: 6685
pFN21AB6189 6685 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2766 3061 2766
Size: 295 6390
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2988 4495 278
Size: 53 83 186 936 1452 1507 2468
Fragments arranged by size:
2468 1507 1452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4028 1937
Size: 2091 4594
Acc65I G'GTAC_C
Cuts at: 4006 4006
Size: 6685
AclI AA'CG_TT
Cuts at: 1563 4812 5185 1563
Size: 3249 373 3063
Fragments arranged by size:
3249 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2795 4748 6132 6400 1632
Size: 542 621 1953 1384 268 1917
Fragments arranged by size:
1953 1917 1384 621 542 268
AflII C'TTAA_G
Cuts at: 820 1017 2500 820
Size: 197 1483 5005
Fragments arranged by size:
5005 1483 197
AflIII A'CryG_T
Cuts at: 1203 3365 3897 5584 6561 1203
Size: 2162 532 1687 977 1327
Fragments arranged by size:
2162 1687 1327 977 532
AgeI A'CCGG_T
Cuts at: 2085 3537 5516 2085
Size: 1452 1979 3254
Fragments arranged by size:
3254 1979 1452
AhdI GACnn_n'nnGTC
Cuts at: 2316 5414 2316
Size: 3098 3587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6685
AlwNI CAG_nnn'CTG
Cuts at: 3929 4428 6000 3929
Size: 499 1572 4614
Fragments arranged by size:
4614 1572 499
ApaLI G'TGCA_C
Cuts at: 3245 4742 5898 3245
Size: 1497 1156 4032
Fragments arranged by size:
4032 1497 1156
ApoI r'AATT_y
Cuts at: 1493 2122 2146 2681 2768 2858 3047 3994 4220
Size: 629 24 535 87 90 189 947 226
Cuts at: 4220 4372 6481 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 947 629 535 226 189 152
90 87 24
AseI AT'TA_AT
Cuts at: 160 1032 2490 5239 160
Size: 872 1458 2749 1606
Fragments arranged by size:
2749 1606 1458 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
AvaI C'yCGr_G
Cuts at: 1870 1943 3306 3681 4010 1870
Size: 73 1363 375 329 4545
Fragments arranged by size:
4545 1363 375 329 73
BamHI G'GATC_C
Cuts at: 4015 4382 4015
Size: 367 6318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4006 5461 618
Size: 325 217 627 2219 1455 1842
Fragments arranged by size:
2219 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4004 1391
Size: 2613 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2180 2528 3304 3351 928
Size: 971 281 348 776 47 4262
Fragments arranged by size:
4262 971 776 348 281 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2731 2765 4876 4910 1903
Size: 34 794 34 2111 34 3678
Fragments arranged by size:
3678 2111 794 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4577 5787 4577
Size: 1210 5475
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6685
BfrBI ATG'CAT
Cuts at: 2626 2978 2626
Size: 352 6333
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5296 136
Size: 107 122 71 631 605 3624 1525
Fragments arranged by size:
3624 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6680 6680
Size: 6685
BlpI GC'TnA_GC
Cuts at: 4102 4102
Size: 6685
Bme1580I G_kGCm'C
Cuts at: 1790 3249 4746 5902 1790
Size: 1459 1497 1156 2573
Fragments arranged by size:
2573 1497 1459 1156
BmgBI CAC'GTC
Cuts at: 1318 2698 3641 1318
Size: 1380 943 4362
Fragments arranged by size:
4362 1380 943
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2728 2734 4416
Size: 416 53 83 399 24 1311 6 1682
Cuts at: 4416 5374 442
Size: 958 1753
Fragments arranged by size:
1753 1682 1311 958 416 399 83 53
24 6
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2879 5345 1239
Size: 1640 2466 2579
Fragments arranged by size:
2579 2466 1640
Bpu10I CC'TnA_GC
Cuts at: 2702 2702
Size: 6685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4810 5675 5973 4810
Size: 865 298 5522
Fragments arranged by size:
5522 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5348 882
Size: 617 3849 2219
Fragments arranged by size:
3849 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4476 493
Size: 711 3272 2702
Fragments arranged by size:
3272 2702 711
BsaBI GATnn'nnATC
Cuts at: 6679 6679
Size: 6685
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2985 4492 4874 5509
Size: 53 83 186 936 1452 1507 382 635
Cuts at: 5509 275
Size: 1451
Fragments arranged by size:
1507 1452 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2085 3537 5118 5516 5790 5937 6348 2085
Size: 1452 1581 398 274 147 411 2422
Fragments arranged by size:
2422 1581 1452 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2585 2615 3542 3572 1178
Size: 30 165 30 1182 30 927 30 4291
Fragments arranged by size:
4291 1182 927 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2289 2867 1186
Size: 1103 578 5004
Fragments arranged by size:
5004 1103 578
BseYI C'CCAG_C
Cuts at: 5888 6384 5888
Size: 496 6189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3089 1163
Size: 573 1353 4759
Fragments arranged by size:
4759 1353 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4135 4896 5924 1540
Size: 275 186 2134 761 1028 2301
Fragments arranged by size:
2301 2134 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 2703 3249 4746 4831 5902 2030
Size: 673 546 1497 85 1071 2813
Fragments arranged by size:
2813 1497 1071 673 546 85
BsmI GAATG_Cn'
Cuts at: 3484 4196 4289 3484
Size: 712 93 5880
Fragments arranged by size:
5880 712 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2992 2992
Size: 6685
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2703 3249 4004 4746 4831 5902
Size: 399 240 673 546 755 742 85 1071
Cuts at: 5902 1391
Size: 2174
Fragments arranged by size:
2174 1071 755 742 673 546 399 240
85
BspEI T'CCGG_A
Cuts at: 6348 6348
Size: 6685
BspHI T'CATG_A
Cuts at: 3706 4574 6304 3706
Size: 868 1730 4087
Fragments arranged by size:
4087 1730 868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2569 2766 3061 4040 844
Size: 1725 197 295 979 3489
Fragments arranged by size:
3489 1725 979 295 197
BsrBI CCG'CTC
Cuts at: 1394 1823 4572 1394
Size: 429 2749 3507
Fragments arranged by size:
3507 2749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5180 5354 65
Size: 1183 3932 174 1396
Fragments arranged by size:
3932 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 3537 3729 5329 5516 1700
Size: 110 275 1452 192 1600 187 2869
Fragments arranged by size:
2869 1600 1452 275 192 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6685
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6685
BssSI C'ACGA_G
Cuts at: 4745 5757 4745
Size: 1012 5673
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3929 794
Size: 3135 3550
BstBI TT'CG_AA
Cuts at: 2179 5489 2179
Size: 3310 3375
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6685
BstYI r'GATC_y
Cuts at: 1972 2806 3394 4015 4382 4767 4784 6236 6322
Size: 834 588 621 367 385 17 1452 86
Cuts at: 6322 6334 6680 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 834 621 588 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2639 2639
Size: 6685
BtgI C'CryG_G
Cuts at: 513 1065 2004 3423 513
Size: 552 939 1419 3775
Fragments arranged by size:
3775 1419 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2734 487
Size: 683 56 1508 4438
Fragments arranged by size:
4438 1508 683 56
BtsI GCAGTG_nn'
Cuts at: 4201 4995 5015 4201
Size: 794 20 5871
Fragments arranged by size:
5871 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6650
DraI TTT'AAA
Cuts at: 2151 3361 3989 2151
Size: 1210 628 4847
Fragments arranged by size:
4847 1210 628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5692 809
Size: 507 4376 1802
Fragments arranged by size:
4376 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3645 3939 4132
Size: 54 1425 50 275 18 1815 294 193
Cuts at: 4132 5021 6650 8
Size: 889 1629 43
Fragments arranged by size:
1815 1629 1425 889 294 275 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4132 1537
Size: 275 2320 4090
Fragments arranged by size:
4090 2320 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2831 4615 1353
Size: 696 782 1784 3423
Fragments arranged by size:
3423 1784 782 696
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5272 5646 5792 387
Size: 1064 138 3683 374 146 1280
Fragments arranged by size:
3683 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2795 2879 4748 5345 6132 6400
Size: 393 542 621 84 1869 597 787 268
Cuts at: 6400 1239
Size: 1524
Fragments arranged by size:
1869 1524 787 621 597 542 393 268
84
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 2246
Size: 6685
EcoO109I rG'GnC_Cy
Cuts at: 2730 2730
Size: 6685
EcoRI G'AATT_C
Cuts at: 3994 4372 3994
Size: 378 6307
EcoRV GAT'ATC
Cuts at: 3955 3955
Size: 6685
FspI TGC'GCA
Cuts at: 5191 5191
Size: 6685
HaeII r_GCGC'y
Cuts at: 1678 5832 1678
Size: 4154 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2050 2080 2082 2302
Size: 32 135 32 476 2 30 2 220
Cuts at: 2302 2334 2501 2533 2585 2617 3441 3473 5326
Size: 32 167 32 52 32 824 32 1853
Cuts at: 5326 5358 5400 5432 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1853 824 476 220 167 135 52
42 32 32 32 32 32 32 32
32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 2646 4029 4275 669
Size: 988 281 655 53 1383 246 3079
Fragments arranged by size:
3079 1383 988 655 281 246 53
HindIII A'AGCT_T
Cuts at: 748 2922 2930 748
Size: 2174 8 4503
Fragments arranged by size:
4503 2174 8
HpaI GTT'AAC
Cuts at: 4275 4275
Size: 6685
KpnI G_GTAC'C
Cuts at: 4010 4010
Size: 6685
MfeI C'AATT_G
Cuts at: 2525 4284 2525
Size: 1759 4926
MluI A'CGCG_T
Cuts at: 6561 6561
Size: 6685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5799 5983 1305
Size: 4494 184 2007
Fragments arranged by size:
4494 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3647 3941 10
Size: 54 1425 343 1815 294 2754
Fragments arranged by size:
2754 1815 1425 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2878 4643 5002 5161 518
Size: 648 1712 1765 359 159 2042
Fragments arranged by size:
2042 1765 1712 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3596 4101 4778 5926 6171 1606
Size: 1990 505 677 1148 245 2120
Fragments arranged by size:
2120 1990 1148 677 505 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3423 513
Size: 552 939 1419 3775
Fragments arranged by size:
3775 1419 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6685
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6685
NotI GC'GGCC_GC
Cuts at: 4132 4132
Size: 6685
NsiI A_TGCA'T
Cuts at: 2628 2980 2628
Size: 352 6333
NspI r_CATG'y
Cuts at: 1132 1229 2630 2746 3901 4043 5588 1132
Size: 97 1401 116 1155 142 1545 2229
Fragments arranged by size:
2229 1545 1401 1155 142 116 97
PasI CC'CwG_GG
Cuts at: 1348 3104 1348
Size: 1756 4929
PciI A'CATG_T
Cuts at: 3897 5584 3897
Size: 1687 4998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3982 1108
Size: 2874 3811
PmeI GTTT'AAAC
Cuts at: 3989 3989
Size: 6685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 6267 6299 4754
Size: 32 1481 32 5140
Fragments arranged by size:
5140 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2730 2730
Size: 6685
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6685
PsiI TTA'TAA
Cuts at: 2823 4255 2823
Size: 1432 5253
PspXI vC'TCGA_Gb
Cuts at: 3306 3306
Size: 6685
PstI C_TGCA'G
Cuts at: 830 2046 3469 4037 830
Size: 1216 1423 568 3478
Fragments arranged by size:
3478 1423 1216 568
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6685
PvuII CAG'CTG
Cuts at: 3596 3596
Size: 6685
SalI G'TCGA_C
Cuts at: 1936 4027 1936
Size: 2091 4594
SanDI GG'GwC_CC
Cuts at: 2730 2730
Size: 6685
SbfI CC_TGCA'GG
Cuts at: 4037 4037
Size: 6685
ScaI AGT'ACT
Cuts at: 4933 6645 4933
Size: 1712 4973
SfcI C'TryA_G
Cuts at: 826 1045 2042 3465 4033 5168 5849 6040 826
Size: 219 997 1423 568 1135 681 191 1471
Fragments arranged by size:
1471 1423 1135 997 681 568 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6685
SmaI CCC'GGG
Cuts at: 1872 4012 1872
Size: 2140 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2500 3306 3681 4789 5690 5952
Size: 197 926 557 806 375 1108 901 262
Cuts at: 5952 820
Size: 1553
Fragments arranged by size:
1553 1108 926 901 806 557 375 262
197
SnaBI TAC'GTA
Cuts at: 493 4476 493
Size: 3983 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6685
SphI G_CATG'C
Cuts at: 1132 2746 4043 1132
Size: 1614 1297 3774
Fragments arranged by size:
3774 1614 1297
SspI AAT'ATT
Cuts at: 5 52 2173 3261 3652 3885 4609 5
Size: 47 2121 1088 391 233 724 2081
Fragments arranged by size:
2121 2081 1088 724 391 233 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6685
StyI C'CwwG_G
Cuts at: 513 1065 2004 2385 3423 3942 4124 513
Size: 552 939 381 1038 519 182 3074
Fragments arranged by size:
3074 1038 939 552 519 381 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4712 4729 4882 5067 5512 325
Size: 1255 3132 17 153 185 445 1498
Fragments arranged by size:
3132 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4931 6643 96
Size: 274 80 33 51 1443 2954 1712 138
Fragments arranged by size:
2954 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2330 2362 3228 3260 3315 3347 6645 6677 2330
Size: 32 866 32 55 32 3298 32 2338
Fragments arranged by size:
3298 2338 866 55 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3455 1316
Size: 2139 4546
XbaI T'CTAG_A
Cuts at: 4021 4021
Size: 6685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3323 3697 2011
Size: 1312 374 4999
Fragments arranged by size:
4999 1312 374
XhoI C'TCGA_G
Cuts at: 1943 3306 3681 1943
Size: 1363 375 4947
Fragments arranged by size:
4947 1363 375
XmaI C'CCGG_G
Cuts at: 1870 4010 1870
Size: 2140 4545
XmnI GAAnn'nnTTC
Cuts at: 4814 4814
Size: 6685
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2986 4493 276
Size: 53 83 186 936 1452 1507 2468
Fragments arranged by size:
2468 1507 1452 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI