Restriction map for
pFN21AB6183
(Circular) (Six-base) MAPSORT of: pFN21AB6183.seq Check: 1742 from: 1 to: 6682
pFN21AB6183 6682 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2304 3757 2304
Size: 1453 5229
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2558 4492 278
Size: 53 83 186 936 1022 1934 2468
Fragments arranged by size:
2468 1934 1022 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2095 4025 1937
Size: 158 1930 4594
Fragments arranged by size:
4594 1930 158
Acc65I G'GTAC_C
Cuts at: 4003 4003
Size: 6682
AclI AA'CG_TT
Cuts at: 1563 4809 5182 1563
Size: 3246 373 3063
Fragments arranged by size:
3246 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2530 3498 3815 4745 6129 6397 1632
Size: 898 968 317 930 1384 268 1917
Fragments arranged by size:
1917 1384 968 930 898 317 268
AfeI AGC'GCT
Cuts at: 3119 3119
Size: 6682
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6485
AflIII A'CryG_T
Cuts at: 1203 5581 6558 1203
Size: 4378 977 1327
Fragments arranged by size:
4378 1327 977
AgeI A'CCGG_T
Cuts at: 5513 5513
Size: 6682
AhdI GACnn_n'nnGTC
Cuts at: 2761 5411 2761
Size: 2650 4032
AleI CACnn'nnGTG
Cuts at: 1166 2057 2519 3227 1166
Size: 891 462 708 4621
Fragments arranged by size:
4621 891 708 462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2133 3245 1184
Size: 949 1112 4621
Fragments arranged by size:
4621 1112 949
AlwNI CAG_nnn'CTG
Cuts at: 2296 2317 2902 2962 3169 3322 3476 3515 4425
Size: 21 585 60 207 153 154 39 910
Cuts at: 4425 5997 2296
Size: 1572 2981
Fragments arranged by size:
2981 1572 910 585 207 154 153 60
39 21
ApaI G_GGCC'C
Cuts at: 2067 2067
Size: 6682
ApaLI G'TGCA_C
Cuts at: 4739 5895 4739
Size: 1156 5526
ApoI r'AATT_y
Cuts at: 1493 3991 4217 4369 6478 1493
Size: 2498 226 152 2109 1697
Fragments arranged by size:
2498 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5236 160
Size: 872 4204 1606
Fragments arranged by size:
4204 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2209 3090 4007 1870
Size: 73 92 174 881 917 4545
Fragments arranged by size:
4545 917 881 174 92 73
BamHI G'GATC_C
Cuts at: 2255 4012 4379 2255
Size: 1757 367 4558
Fragments arranged by size:
4558 1757 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2359 3221 4003 5458
Size: 325 217 627 258 314 862 782 1455
Cuts at: 5458 618
Size: 1842
Fragments arranged by size:
1842 1455 862 782 627 325 314 258
217
BanII G_rGCy'C
Cuts at: 1391 2067 2226 2922 3023 3342 3642 3903 4001
Size: 676 159 696 101 319 300 261 98
Cuts at: 4001 1391
Size: 4072
Fragments arranged by size:
4072 696 676 319 300 261 159 101
98
BbeI G_GCGC'C
Cuts at: 2049 2049
Size: 6682
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3623 3682 3699 928
Size: 971 1724 59 17 3911
Fragments arranged by size:
3911 1724 971 59 17
BbvCI CC'TCA_GC
Cuts at: 2318 3783 2318
Size: 1465 5217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4873 4907 1903
Size: 34 2936 34 3678
Fragments arranged by size:
3678 2936 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4574 5784 4574
Size: 1210 5472
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6682
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2062 2374 3839
Size: 107 122 71 631 605 390 312 1465
Cuts at: 3839 5293 136
Size: 1454 1525
Fragments arranged by size:
1525 1465 1454 631 605 390 312 122
107 71
BglII A'GATC_T
Cuts at: 3387 6677 3387
Size: 3290 3392
BlpI GC'TnA_GC
Cuts at: 2835 3262 4099 2835
Size: 427 837 5418
Fragments arranged by size:
5418 837 427
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2680 3224 4743 5899 1790
Size: 277 613 544 1519 1156 2573
Fragments arranged by size:
2573 1519 1156 613 544 277
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6682
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4413 5371 442
Size: 416 53 83 399 24 2996 958 1753
Fragments arranged by size:
2996 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3679 3709 3679
Size: 30 6652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2375 2527 2600 2780 2888 2912 2924
Size: 845 291 152 73 180 108 24 12
Cuts at: 2924 3065 3332 3656 3674 3806 5342 1239
Size: 141 267 324 18 132 1536 2579
Fragments arranged by size:
2579 1536 845 324 291 267 180 152
141 132 108 73 24 18 12
Bpu10I CC'TnA_GC
Cuts at: 2318 3114 3783 2318
Size: 796 669 5217
Fragments arranged by size:
5217 796 669
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4807 5672 5970 4807
Size: 865 298 5519
Fragments arranged by size:
5519 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3457 3662 5345 882
Size: 617 1958 205 1683 2219
Fragments arranged by size:
2219 1958 1683 617 205
BsaAI yAC'GTr
Cuts at: 493 1204 3229 3331 3596 4473 493
Size: 711 2025 102 265 877 2702
Fragments arranged by size:
2702 2025 877 711 265 102
BsaBI GATnn'nnATC
Cuts at: 6676 6676
Size: 6682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2555 3252 4489
Size: 53 83 186 936 513 509 697 1237
Cuts at: 4489 4871 5506 275
Size: 382 635 1451
Fragments arranged by size:
1451 1237 936 697 635 513 509 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3846 3927 5115 5513 5787 5934 6345 3846
Size: 81 1188 398 274 147 411 4183
Fragments arranged by size:
4183 1188 411 398 274 147 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 2975 3005 1178
Size: 30 165 30 1050 30 492 30 4855
Fragments arranged by size:
4855 1050 492 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2234 2294 3062 3167 3476 3545 3983
Size: 1045 3 60 768 105 309 69 438
Cuts at: 3983 1186
Size: 3885
Fragments arranged by size:
3885 1045 768 438 309 105 69 60
3
BseYI C'CCAG_C
Cuts at: 2349 2474 2777 3162 3469 5885 6381 2349
Size: 125 303 385 307 2416 496 2650
Fragments arranged by size:
2650 2416 496 385 307 303 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 2464 3353 1163
Size: 573 546 182 889 4492
Fragments arranged by size:
4492 889 573 546 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4132 4893 5921 1540
Size: 275 186 2131 761 1028 2301
Fragments arranged by size:
2301 2131 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2226 3217 3342 3468 3662 4743 4828 5899
Size: 99 991 125 126 194 1081 85 1071
Cuts at: 5899 2127
Size: 2910
Fragments arranged by size:
2910 1081 1071 991 194 126 125 99
85
BsmI GAATG_Cn'
Cuts at: 3308 4193 4286 3308
Size: 885 93 5704
Fragments arranged by size:
5704 885 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2426 2529 3179 2426
Size: 103 650 5929
Fragments arranged by size:
5929 650 103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2127 2226 2680 2922 3023 3217
Size: 399 277 60 99 454 242 101 194
Cuts at: 3217 3224 3342 3468 3642 3662 3903 4001 4743
Size: 7 118 126 174 20 241 98 742
Cuts at: 4743 4828 5899 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 277 242 241
194 174 126 118 101 99 98 85
60 20 7
BspEI T'CCGG_A
Cuts at: 3927 6345 3927
Size: 2418 4264
BspHI T'CATG_A
Cuts at: 3615 4571 6301 3615
Size: 956 1730 3996
Fragments arranged by size:
3996 1730 956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2304 2394 3757 4037 844
Size: 1460 90 1363 280 3489
Fragments arranged by size:
3489 1460 1363 280 90
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 4569 1394
Size: 429 1438 1308 3507
Fragments arranged by size:
3507 1438 1308 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5177 5351 65
Size: 1183 3929 174 1396
Fragments arranged by size:
3929 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5326 5513 1700
Size: 110 3516 187 2869
Fragments arranged by size:
3516 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6682
BssSI C'ACGA_G
Cuts at: 2403 3701 4742 5754 2403
Size: 1298 1041 1012 3331
Fragments arranged by size:
3331 1298 1041 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2296 3322 794
Size: 1502 1026 4154
Fragments arranged by size:
4154 1502 1026
BstBI TT'CG_AA
Cuts at: 5486 5486
Size: 6682
BstEII G'GTnAC_C
Cuts at: 1184 3067 3814 1184
Size: 1883 747 4052
Fragments arranged by size:
4052 1883 747
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2059 3277 2011
Size: 48 1218 5416
Fragments arranged by size:
5416 1218 48
BstYI r'GATC_y
Cuts at: 1972 2255 2574 2889 3387 4012 4379 4764 4781
Size: 283 319 315 498 625 367 385 17
Cuts at: 4781 6233 6319 6331 6677 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 625 498 385 367 346 319
315 283 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3516 3516
Size: 6682
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2128 2227 3587 3834 513
Size: 552 939 54 70 99 1360 247 3361
Fragments arranged by size:
3361 1360 939 552 247 99 70 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3334 487
Size: 683 56 2108 3835
Fragments arranged by size:
3835 2108 683 56
BtsI GCAGTG_nn'
Cuts at: 2680 3238 3835 4198 4992 5012 2680
Size: 558 597 363 794 20 4350
Fragments arranged by size:
4350 794 597 558 363 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6647
DraI TTT'AAA
Cuts at: 3986 3986
Size: 6682
DraIII CAC_nnn'GTG
Cuts at: 3136 3136
Size: 6682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2078 5689 809
Size: 507 762 3611 1802
Fragments arranged by size:
3611 1802 762 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2751 3267 3408
Size: 54 1425 50 275 18 921 516 141
Cuts at: 3408 3590 3840 4129 5018 6647 8
Size: 182 250 289 889 1629 43
Fragments arranged by size:
1629 1425 921 889 516 289 275 250
182 141 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4129 1537
Size: 275 2317 4090
Fragments arranged by size:
4090 2317 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3507 3546 3570 3700 3711 4612 1353
Size: 2154 39 24 130 11 901 3423
Fragments arranged by size:
3423 2154 901 130 39 24 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2327 2882 3495 5269 5643 5789
Size: 1064 138 738 555 613 1774 374 146
Cuts at: 5789 387
Size: 1280
Fragments arranged by size:
1774 1280 1064 738 613 555 374 146
138
EcoICRI GAG'CTC
Cuts at: 2224 3340 2224
Size: 1116 5566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2375 2527 2530 2600 2780 2888
Size: 393 452 291 152 3 70 180 108
Cuts at: 2888 2912 2924 3065 3332 3498 3656 3674 3806
Size: 24 12 141 267 166 158 18 132
Cuts at: 3806 3815 4745 5342 6129 6397 1239
Size: 9 930 597 787 268 1524
Fragments arranged by size:
1524 930 787 597 452 393 291 268
267 180 166 158 152 141 132 108
70 24 18 12 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3314 3314
Size: 6682
EcoO109I rG'GnC_Cy
Cuts at: 2063 2064 2206 2796 3319 3779 2063
Size: 1 142 590 523 460 4966
Fragments arranged by size:
4966 590 523 460 142 1
EcoRI G'AATT_C
Cuts at: 3991 4369 3991
Size: 378 6304
FspI TGC'GCA
Cuts at: 5188 5188
Size: 6682
HaeII r_GCGC'y
Cuts at: 1678 2049 2784 3121 5829 1678
Size: 371 735 337 2708 2531
Fragments arranged by size:
2708 2531 735 371 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2747 2779 2975
Size: 32 135 32 881 32 262 32 196
Cuts at: 2975 3007 3140 3172 5323 5355 5397 5429 1373
Size: 32 133 32 2151 32 42 32 2626
Fragments arranged by size:
2626 2151 881 262 196 135 133 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2096 4026 4272 669
Size: 988 281 158 1930 246 3079
Fragments arranged by size:
3079 1930 988 281 246 158
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6682
HpaI GTT'AAC
Cuts at: 4272 4272
Size: 6682
KasI G'GCGC_C
Cuts at: 2045 2045
Size: 6682
KpnI G_GTAC'C
Cuts at: 4007 4007
Size: 6682
MfeI C'AATT_G
Cuts at: 4281 4281
Size: 6682
MluI A'CGCG_T
Cuts at: 6558 6558
Size: 6682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2832 5796 5980 1305
Size: 1527 2964 184 2007
Fragments arranged by size:
2964 2007 1527 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3842 10
Size: 54 1425 343 2010 2850
Fragments arranged by size:
2850 2010 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2519 3227 3960 4640 4999 5158
Size: 648 891 462 708 733 680 359 159
Cuts at: 5158 518
Size: 2042
Fragments arranged by size:
2042 891 733 708 680 648 462 359
159
MspA1I CmG'CkG
Cuts at: 1606 2043 2314 2353 2478 2655 2980 3166 3266
Size: 437 271 39 125 177 325 186 100
Cuts at: 3266 3382 4098 4775 5923 6168 1606
Size: 116 716 677 1148 245 2120
Fragments arranged by size:
2120 1148 716 677 437 325 271 245
186 177 125 116 100 39
NarI GG'CG_CC
Cuts at: 2046 2046
Size: 6682
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5191
Fragments arranged by size:
5191 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6682
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6682
NotI GC'GGCC_GC
Cuts at: 4129 4129
Size: 6682
NspI r_CATG'y
Cuts at: 1132 1229 2637 3682 4040 5585 1132
Size: 97 1408 1045 358 1545 2229
Fragments arranged by size:
2229 1545 1408 1045 358 97
PasI CC'CwG_GG
Cuts at: 1348 2067 2485 1348
Size: 719 418 5545
Fragments arranged by size:
5545 719 418
PciI A'CATG_T
Cuts at: 5581 5581
Size: 6682
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2902 3232 3769 3811 1108
Size: 1794 330 537 42 3979
Fragments arranged by size:
3979 1794 537 330 42
PfoI T'CCnGG_A
Cuts at: 3861 3861
Size: 6682
PmeI GTTT'AAAC
Cuts at: 3986 3986
Size: 6682
PmlI CAC'GTG
Cuts at: 3229 3331 3229
Size: 102 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4751 4783 6264 6296 4751
Size: 32 1481 32 5137
Fragments arranged by size:
5137 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2796 2796
Size: 6682
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6682
PsiI TTA'TAA
Cuts at: 4252 4252
Size: 6682
PspOMI G'GGCC_C
Cuts at: 2063 2063
Size: 6682
PstI C_TGCA'G
Cuts at: 830 2151 3057 3387 3729 4034 830
Size: 1321 906 330 342 305 3478
Fragments arranged by size:
3478 1321 906 342 330 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6682
PvuII CAG'CTG
Cuts at: 2314 2353 2478 3166 3382 2314
Size: 39 125 688 216 5614
Fragments arranged by size:
5614 688 216 125 39
SacI G_AGCT'C
Cuts at: 2226 3342 2226
Size: 1116 5566
SalI G'TCGA_C
Cuts at: 1936 2094 4024 1936
Size: 158 1930 4594
Fragments arranged by size:
4594 1930 158
SapI GCTCTTCn'nnn_
Cuts at: 3570 3711 3570
Size: 141 6541
SbfI CC_TGCA'GG
Cuts at: 4034 4034
Size: 6682
ScaI AGT'ACT
Cuts at: 4930 6642 4930
Size: 1712 4970
SfcI C'TryA_G
Cuts at: 826 1045 2147 3053 3383 3725 4030 5165 5846
Size: 219 1102 906 330 342 305 1135 681
Cuts at: 5846 6037 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1102 906 681 342 330 305
219 191
SfoI GGC'GCC
Cuts at: 2047 2047
Size: 6682
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6682
SmaI CCC'GGG
Cuts at: 1872 2037 3092 4009 1872
Size: 165 1055 917 4545
Fragments arranged by size:
4545 1055 917 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4786 5687 5949 820
Size: 197 926 2843 901 262 1553
Fragments arranged by size:
2843 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4473 493
Size: 3980 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6682
SphI G_CATG'C
Cuts at: 1132 4040 1132
Size: 2908 3774
SspI AAT'ATT
Cuts at: 5 52 4606 5
Size: 47 4554 2081
Fragments arranged by size:
4554 2081 47
StuI AGG'CCT
Cuts at: 1517 3857 1517
Size: 2340 4342
StyI C'CwwG_G
Cuts at: 513 1065 2004 3645 3753 4121 513
Size: 552 939 1641 108 368 3074
Fragments arranged by size:
3074 1641 939 552 368 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2531 3000 3086 3239 3434 3483 4709
Size: 1255 951 469 86 153 195 49 1226
Cuts at: 4709 4726 4879 5064 5509 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1226 951 469 445 195 185
153 153 86 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 4928 6640
Size: 274 80 33 51 1443 546 2405 1712
Cuts at: 6640 96
Size: 138
Fragments arranged by size:
2405 1712 1443 546 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6642 6674 6642
Size: 32 6650
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6682
XbaI T'CTAG_A
Cuts at: 4018 4018
Size: 6682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2482 3631 2482
Size: 1149 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6682
XmaI C'CCGG_G
Cuts at: 1870 2035 3090 4007 1870
Size: 165 1055 917 4545
Fragments arranged by size:
4545 1055 917 165
XmnI GAAnn'nnTTC
Cuts at: 4811 4811
Size: 6682
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2556 4490 276
Size: 53 83 186 936 1022 1934 2468
Fragments arranged by size:
2468 1934 1022 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI