Restriction map for
pFN21AB6176
(Circular) (Six-base) MAPSORT of: pFN21AB6176.seq Check: 4707 from: 1 to: 6523
pFN21AB6176 6523 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3061 3192 3061
Size: 131 6392
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3480 4333 278
Size: 53 83 186 936 1944 853 2468
Fragments arranged by size:
2468 1944 936 853 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2073 2127 2187 3866 1937
Size: 136 54 60 1679 4594
Fragments arranged by size:
4594 1679 136 60 54
Acc65I G'GTAC_C
Cuts at: 2889 2917 3844 2889
Size: 28 927 5568
Fragments arranged by size:
5568 927 28
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2308 2963 4586 5970 6238 1632
Size: 539 137 655 1623 1384 268 1917
Fragments arranged by size:
1917 1623 1384 655 539 268 137
AflII C'TTAA_G
Cuts at: 820 1017 2351 820
Size: 197 1334 4992
Fragments arranged by size:
4992 1334 197
AflIII A'CryG_T
Cuts at: 1203 3091 5422 6399 1203
Size: 1888 2331 977 1327
Fragments arranged by size:
2331 1888 1327 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 2099 3154 1166
Size: 933 1055 4535
Fragments arranged by size:
4535 1055 933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AlwNI CAG_nnn'CTG
Cuts at: 2941 3410 3615 3694 4266 5838 2941
Size: 469 205 79 572 1572 3626
Fragments arranged by size:
3626 1572 572 469 205 79
ApaI G_GGCC'C
Cuts at: 2024 3385 2024
Size: 1361 5162
ApaLI G'TGCA_C
Cuts at: 4580 5736 4580
Size: 1156 5367
ApoI r'AATT_y
Cuts at: 1493 3832 4058 4210 6319 1493
Size: 2339 226 152 2109 1697
Fragments arranged by size:
2339 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5077 160
Size: 872 4045 1606
Fragments arranged by size:
4045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2766 2797 3848 1870
Size: 73 106 717 31 1051 4545
Fragments arranged by size:
4545 1051 717 106 73 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2431 2464 2431
Size: 33 6490
BamHI G'GATC_C
Cuts at: 3853 4220 3853
Size: 367 6156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2889 2917 2969 3633
Size: 325 217 627 273 829 28 52 664
Cuts at: 3633 3844 5299 618
Size: 211 1455 1842
Fragments arranged by size:
1842 1455 829 664 627 325 273 217
211 52 28
BanII G_rGCy'C
Cuts at: 1391 2024 2262 2777 2963 3167 3327 3385 3842
Size: 633 238 515 186 204 160 58 457
Cuts at: 3842 1391
Size: 4072
Fragments arranged by size:
4072 633 515 457 238 204 186 160
58
BbeI G_GCGC'C
Cuts at: 3637 3637
Size: 6523
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2321 3089 3371 928
Size: 971 422 768 282 4080
Fragments arranged by size:
4080 971 768 422 282
BbvCI CC'TCA_GC
Cuts at: 3429 3596 3429
Size: 167 6356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4714 4748 1903
Size: 34 2777 34 3678
Fragments arranged by size:
3678 2777 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4415 5625 4415
Size: 1210 5313
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6523
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 3540 5134
Size: 107 122 71 631 605 364 1504 1594
Cuts at: 5134 136
Size: 1525
Fragments arranged by size:
1594 1525 1504 631 605 364 122 107
71
BglII A'GATC_T
Cuts at: 3289 6518 3289
Size: 3229 3294
BlpI GC'TnA_GC
Cuts at: 3039 3940 3039
Size: 901 5622
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2063 3385 4584 5740 1790
Size: 234 39 1322 1199 1156 2573
Fragments arranged by size:
2573 1322 1199 1156 234 39
BmgBI CAC'GTC
Cuts at: 1318 2643 3229 1318
Size: 1325 586 4612
Fragments arranged by size:
4612 1325 586
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2919 2920 4254
Size: 416 53 83 399 24 1502 1 1334
Cuts at: 4254 5212 442
Size: 958 1753
Fragments arranged by size:
1753 1502 1334 958 416 399 83 53
24 1
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 3418 2533
Size: 885 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2482 3151 3592 5183 1239
Size: 1243 669 441 1591 2579
Fragments arranged by size:
2579 1591 1243 669 441
Bpu10I CC'TnA_GC
Cuts at: 3077 3429 3596 3077
Size: 352 167 6004
Fragments arranged by size:
6004 352 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3278 3638 4648 5513 5811 3002
Size: 276 360 1010 865 298 3714
Fragments arranged by size:
3714 1010 865 360 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5186 882
Size: 617 3687 2219
Fragments arranged by size:
3687 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4314 493
Size: 711 3110 2702
Fragments arranged by size:
3110 2702 711
BsaBI GATnn'nnATC
Cuts at: 6517 6517
Size: 6523
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3477 3634 4330 4712
Size: 53 83 186 936 1944 157 696 382
Cuts at: 4712 5347 275
Size: 635 1451
Fragments arranged by size:
1944 1451 936 696 635 382 186 157
83 53
BsaWI w'CCGG_w
Cuts at: 2489 4956 5354 5628 5775 6186 2489
Size: 2467 398 274 147 411 2826
Fragments arranged by size:
2826 2467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2632 2662 3708 3738 1178
Size: 30 165 30 1229 30 1046 30 3963
Fragments arranged by size:
3963 1229 1046 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2760 3683 1186
Size: 1084 490 923 4026
Fragments arranged by size:
4026 1084 923 490
BseYI C'CCAG_C
Cuts at: 2558 2571 2608 2777 2782 3027 3384 5726 6222
Size: 13 37 169 5 245 357 2342 496
Cuts at: 6222 2558
Size: 2859
Fragments arranged by size:
2859 2342 496 357 245 169 37 13
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 1163
Size: 573 1218 4732
Fragments arranged by size:
4732 1218 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3131 3973 4734 5762 1540
Size: 275 186 1130 842 761 1028 2301
Fragments arranged by size:
2301 1130 1028 842 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2592 3018 3078 3501 4584 4669 5740 2262
Size: 330 426 60 423 1083 85 1071 3045
Fragments arranged by size:
3045 1083 1071 426 423 330 85 60
BsmI GAATG_Cn'
Cuts at: 4034 4127 4034
Size: 93 6430
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2063 2262 2592 2777 2963 3018
Size: 399 234 39 199 330 185 186 55
Cuts at: 3018 3078 3167 3327 3385 3501 3842 4584 4669
Size: 60 89 160 58 116 341 742 85
Cuts at: 4669 5740 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 330 234 199
186 185 160 116 89 85 60 58
55 39
BspEI T'CCGG_A
Cuts at: 2489 6186 2489
Size: 3697 2826
BspHI T'CATG_A
Cuts at: 3120 4412 6142 3120
Size: 1292 1730 3501
Fragments arranged by size:
3501 1730 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 3061 3192 3878 844
Size: 1241 976 131 686 3489
Fragments arranged by size:
3489 1241 976 686 131
BsrBI CCG'CTC
Cuts at: 1394 1823 2637 2845 3378 3673 3739 4410 1394
Size: 429 814 208 533 295 66 671 3507
Fragments arranged by size:
3507 814 671 533 429 295 208 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 2235 5018 5192 65
Size: 1183 987 2783 174 1396
Fragments arranged by size:
2783 1396 1183 987 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2735 5167 5354 1700
Size: 110 925 2432 187 2869
Fragments arranged by size:
2869 2432 925 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 4583 5595 4583
Size: 1012 5511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6523
BstBI TT'CG_AA
Cuts at: 5327 5327
Size: 6523
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6523
BstXI CCAn_nnnn'nTGG
Cuts at: 2387 2708 2387
Size: 321 6202
BstYI r'GATC_y
Cuts at: 1972 3289 3407 3690 3853 4220 4605 4622 6074
Size: 1317 118 283 163 367 385 17 1452
Cuts at: 6074 6160 6172 6518 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1317 385 367 346 283 163
118 86 17 12
BstZ17I GTA'TAC
Cuts at: 2074 2074
Size: 6523
Bsu36I CC'TnA_GG
Cuts at: 3403 3403
Size: 6523
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 2365 2673 2682 3574 513
Size: 552 939 11 350 308 9 892 3462
Fragments arranged by size:
3462 939 892 552 350 308 11 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3012 487
Size: 683 56 1786 3998
Fragments arranged by size:
3998 1786 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2217 3521 4039 4833 4853 2061
Size: 156 1304 518 794 20 3731
Fragments arranged by size:
3731 1304 794 518 156 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2390 2425 529
Size: 35 1826 35 4627
Fragments arranged by size:
4627 1826 35 35
DraI TTT'AAA
Cuts at: 3827 3827
Size: 6523
DraIII CAC_nnn'GTG
Cuts at: 2927 3153 2927
Size: 226 6297
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5530 809
Size: 507 4214 1802
Fragments arranged by size:
4214 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2710 2856 3003
Size: 54 1425 50 275 18 880 146 147
Cuts at: 3003 3128 3192 3354 3696 3970 4859 6488 8
Size: 125 64 162 342 274 889 1629 43
Fragments arranged by size:
1629 1425 889 880 342 275 274 162
147 146 125 64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3128 3970 1537
Size: 275 1316 842 4090
Fragments arranged by size:
4090 1316 842 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2939 3686 4453 1353
Size: 1586 747 767 3423
Fragments arranged by size:
3423 1586 767 747
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5110 5484 5630 387
Size: 1064 138 3521 374 146 1280
Fragments arranged by size:
3521 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2260 2260
Size: 6523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2308 2482 2963 3151 3592 4586
Size: 393 539 137 174 481 188 441 994
Cuts at: 4586 5183 5970 6238 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 994 787 597 539 481 441 393
268 188 174 137
EcoNI CCTnn'n_nnAGG
Cuts at: 2800 3317 2800
Size: 517 6006
EcoO109I rG'GnC_Cy
Cuts at: 2020 2021 2146 2272 2422 2555 3381 3448 2020
Size: 1 125 126 150 133 826 67 5095
Fragments arranged by size:
5095 826 150 133 126 125 67 1
EcoRI G'AATT_C
Cuts at: 3832 4210 3832
Size: 378 6145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 6523
FspI TGC'GCA
Cuts at: 5029 5029
Size: 6523
HaeII r_GCGC'y
Cuts at: 1678 3637 5670 1678
Size: 1959 2033 2531
Fragments arranged by size:
2531 2033 1959
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2834 2866 3126 3158 3281
Size: 32 135 32 1262 32 260 32 123
Cuts at: 3281 3313 5164 5196 5238 5270 1373
Size: 32 1851 32 42 32 2626
Fragments arranged by size:
2626 1851 1262 260 135 123 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2440 3097 3867 4113 669
Size: 988 281 502 657 770 246 3079
Fragments arranged by size:
3079 988 770 657 502 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6523
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KasI G'GCGC_C
Cuts at: 3633 3633
Size: 6523
KpnI G_GTAC'C
Cuts at: 2893 2921 3848 2893
Size: 28 927 5568
Fragments arranged by size:
5568 927 28
MfeI C'AATT_G
Cuts at: 3702 4122 3702
Size: 420 6103
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2715 5637 5821 1305
Size: 1410 2922 184 2007
Fragments arranged by size:
2922 2007 1410 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2712 3356 3698 10
Size: 54 1425 343 880 644 342 2835
Fragments arranged by size:
2835 1425 880 644 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2997 3154 3186 3227 4481 4840
Size: 648 933 898 157 32 41 1254 359
Cuts at: 4840 4999 518
Size: 159 2042
Fragments arranged by size:
2042 1254 933 898 648 359 159 157
41 32
MspA1I CmG'CkG
Cuts at: 1606 2290 2938 3038 3428 3664 3939 4616 5764
Size: 684 648 100 390 236 275 677 1148
Cuts at: 5764 6009 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 684 677 648 390 275 245
236 100
NarI GG'CG_CC
Cuts at: 3634 3634
Size: 6523
NcoI C'CATG_G
Cuts at: 513 1065 2004 2365 2682 513
Size: 552 939 361 317 4354
Fragments arranged by size:
4354 939 552 361 317
NdeI CA'TA_TG
Cuts at: 387 2661 387
Size: 2274 4249
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 3095 3191 3881 5426 1132
Size: 97 1866 96 690 1545 2229
Fragments arranged by size:
2229 1866 1545 690 97 96
PasI CC'CwG_GG
Cuts at: 1348 2551 3247 1348
Size: 1203 696 4624
Fragments arranged by size:
4624 1203 696
PciI A'CATG_T
Cuts at: 3091 5422 3091
Size: 2331 4192
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3253 3391 3694 1108
Size: 2145 138 303 3937
Fragments arranged by size:
3937 2145 303 138
PfoI T'CCnGG_A
Cuts at: 2109 2478 2109
Size: 369 6154
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 6105 6137 4592
Size: 32 1481 32 4978
Fragments arranged by size:
4978 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 2422 2555 3448 2272
Size: 150 133 893 5347
Fragments arranged by size:
5347 893 150 133
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6523
PsiI TTA'TAA
Cuts at: 4093 4093
Size: 6523
PspOMI G'GGCC_C
Cuts at: 2020 3381 2020
Size: 1361 5162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 6491
PstI C_TGCA'G
Cuts at: 830 3875 830
Size: 3045 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
PvuII CAG'CTG
Cuts at: 2938 3038 3428 2938
Size: 100 390 6033
Fragments arranged by size:
6033 390 100
SacI G_AGCT'C
Cuts at: 2262 2262
Size: 6523
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SanDI GG'GwC_CC
Cuts at: 2555 2555
Size: 6523
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 4771 6483 4771
Size: 1712 4811
SfcI C'TryA_G
Cuts at: 826 1045 2624 3460 3871 5006 5687 5878 826
Size: 219 1579 836 411 1135 681 191 1471
Fragments arranged by size:
1579 1471 1135 836 681 411 219 191
SfoI GGC'GCC
Cuts at: 3635 3635
Size: 6523
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 2051 2768 3850 1872
Size: 179 717 1082 4545
Fragments arranged by size:
4545 1082 717 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 3017 3293 3653 4627 5528
Size: 197 926 408 666 276 360 974 901
Cuts at: 5528 5790 820
Size: 262 1553
Fragments arranged by size:
1553 974 926 901 666 408 360 276
262 197
SnaBI TAC'GTA
Cuts at: 493 4314 493
Size: 3821 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6523
SphI G_CATG'C
Cuts at: 1132 3881 1132
Size: 2749 3774
SspI AAT'ATT
Cuts at: 5 52 4447 5
Size: 47 4395 2081
Fragments arranged by size:
4395 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6523
StyI C'CwwG_G
Cuts at: 513 1065 2004 2365 2418 2682 2853 3390 3621
Size: 552 939 361 53 264 171 537 231
Cuts at: 3621 3962 513
Size: 341 3074
Fragments arranged by size:
3074 939 552 537 361 341 264 231
171 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2585 2938 3785 4550 4567 4720 4905
Size: 1255 1005 353 847 765 17 153 185
Cuts at: 4905 5350 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1005 847 765 445 353 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4769 6481 96
Size: 274 80 33 51 1443 2792 1712 138
Fragments arranged by size:
2792 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2632 2664 6483 6515 2632
Size: 32 3819 32 2640
Fragments arranged by size:
3819 2640 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3212 1316
Size: 1896 4627
XbaI T'CTAG_A
Cuts at: 2137 3859 2137
Size: 1722 4801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 2659 2680 2242
Size: 417 21 6085
Fragments arranged by size:
6085 417 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6523
XmaI C'CCGG_G
Cuts at: 1870 2049 2766 3848 1870
Size: 179 717 1082 4545
Fragments arranged by size:
4545 1082 717 179
XmnI GAAnn'nnTTC
Cuts at: 2318 4652 2318
Size: 2334 4189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3478 4331 276
Size: 53 83 186 936 1944 853 2468
Fragments arranged by size:
2468 1944 936 853 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BsmBI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI RsrII SacII SapI SexAI SfiI SrfI SwaI