Restriction map for
pFN21AB6175
(Circular) (Six-base) MAPSORT of: pFN21AB6175.seq Check: 90 from: 1 to: 6664
pFN21AB6175 6664 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2505 3258 2505
Size: 753 5911
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4007 1937
Size: 2070 4594
Acc65I G'GTAC_C
Cuts at: 3985 3985
Size: 6664
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2431 2648 2732 3495 4727 6111 6379 1632
Size: 799 217 84 763 1232 1384 268 1917
Fragments arranged by size:
1917 1384 1232 799 763 268 217 84
AfeI AGC'GCT
Cuts at: 2540 2981 2540
Size: 441 6223
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6467
AflIII A'CryG_T
Cuts at: 1203 3774 5563 6540 1203
Size: 2571 1789 977 1327
Fragments arranged by size:
2571 1789 1327 977
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 2609 3777 3806 1166
Size: 1443 1168 29 4024
Fragments arranged by size:
4024 1443 1168 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2627 3745 1184
Size: 1443 1118 4103
Fragments arranged by size:
4103 1443 1118
AlwNI CAG_nnn'CTG
Cuts at: 2497 2809 3711 3790 4407 5979 2497
Size: 312 902 79 617 1572 3182
Fragments arranged by size:
3182 1572 902 617 312 79
ApaI G_GGCC'C
Cuts at: 2518 3347 3595 3800 3860 2518
Size: 829 248 205 60 5322
Fragments arranged by size:
5322 829 248 205 60
ApaLI G'TGCA_C
Cuts at: 2424 2431 2754 4721 5877 2424
Size: 7 323 1967 1156 3211
Fragments arranged by size:
3211 1967 1156 323 7
ApoI r'AATT_y
Cuts at: 1493 3973 4199 4351 6460 1493
Size: 2480 226 152 2109 1697
Fragments arranged by size:
2480 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5218 160
Size: 872 4186 1606
Fragments arranged by size:
4186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2130 2936 3090 3989 1870
Size: 73 104 83 806 154 899 4545
Fragments arranged by size:
4545 899 806 154 104 83 73
BamHI G'GATC_C
Cuts at: 2051 3994 4361 2051
Size: 1943 367 4354
Fragments arranged by size:
4354 1943 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3124 3270 3823 3985 5440
Size: 325 217 627 1337 146 553 162 1455
Cuts at: 5440 618
Size: 1842
Fragments arranged by size:
1842 1455 1337 627 553 325 217 162
146
BanII G_rGCy'C
Cuts at: 1391 2518 2745 3297 3347 3561 3595 3800 3860
Size: 1127 227 552 50 214 34 205 60
Cuts at: 3860 3983 1391
Size: 123 4072
Fragments arranged by size:
4072 1127 552 227 214 205 123 60
50 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2219 3245 928
Size: 971 320 1026 4347
Fragments arranged by size:
4347 1026 971 320
BbvCI CC'TCA_GC
Cuts at: 2182 2945 3399 2182
Size: 763 454 5447
Fragments arranged by size:
5447 763 454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4855 4889 1903
Size: 34 50 34 2834 34 3678
Fragments arranged by size:
3678 2834 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4556 5766 4556
Size: 1210 5454
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6664
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2513 3795
Size: 107 122 71 631 605 337 504 1282
Cuts at: 3795 3812 3939 5275 136
Size: 17 127 1336 1525
Fragments arranged by size:
1525 1336 1282 631 605 504 337 127
122 107 71 17
BglII A'GATC_T
Cuts at: 6659 6659
Size: 6664
BlpI GC'TnA_GC
Cuts at: 2525 4081 2525
Size: 1556 5108
Bme1580I G_kGCm'C
Cuts at: 1790 2428 2435 2518 2758 3347 3595 3800 3860
Size: 638 7 83 240 589 248 205 60
Cuts at: 3860 3960 4725 5881 1790
Size: 100 765 1156 2573
Fragments arranged by size:
2573 1156 765 638 589 248 240 205
100 83 60 7
BmgBI CAC'GTC
Cuts at: 1318 3409 3702 1318
Size: 2091 293 4280
Fragments arranged by size:
4280 2091 293
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2153 2776 3313
Size: 416 53 83 399 24 736 623 537
Cuts at: 3313 3766 4395 5353 442
Size: 453 629 958 1753
Fragments arranged by size:
1753 958 736 629 623 537 453 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 3056 3484 3643 5324 1239
Size: 872 945 428 159 1681 2579
Fragments arranged by size:
2579 1681 945 872 428 159
Bpu10I CC'TnA_GC
Cuts at: 2175 2182 2945 3399 2175
Size: 7 763 454 5440
Fragments arranged by size:
5440 763 454 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2519 4789 5654 5952 2519
Size: 2270 865 298 3231
Fragments arranged by size:
3231 2270 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5327 882
Size: 617 3828 2219
Fragments arranged by size:
3828 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3448 3775 4455 493
Size: 711 2244 327 680 2702
Fragments arranged by size:
2702 2244 711 680 327
BsaBI GATnn'nnATC
Cuts at: 6658 6658
Size: 6664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2774 2817 2888 3324
Size: 53 83 186 936 1241 43 71 436
Cuts at: 3324 3627 4471 4853 5488 275
Size: 303 844 382 635 1451
Fragments arranged by size:
1451 1241 936 844 635 436 382 303
186 83 71 53 43
BsaWI w'CCGG_w
Cuts at: 5097 5495 5769 5916 6327 5097
Size: 398 274 147 411 5434
Fragments arranged by size:
5434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6439
Fragments arranged by size:
6439 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2660 2818 2833 2896 3305 1186
Size: 1474 158 15 63 409 4545
Fragments arranged by size:
4545 1474 409 158 63 15
BseYI C'CCAG_C
Cuts at: 2152 2200 2237 2503 2614 2793 2976 2990 3887
Size: 48 37 266 111 179 183 14 897
Cuts at: 3887 3929 5867 6363 2152
Size: 42 1938 496 2453
Fragments arranged by size:
2453 1938 897 496 266 183 179 111
48 42 37 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2732 2915 3724 1163
Size: 573 996 183 809 4103
Fragments arranged by size:
4103 996 809 573 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 2643 2693 3116 3286 4114
Size: 275 186 343 299 50 423 170 828
Cuts at: 4114 4875 5903 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 828 761 423 343 299 275
186 170 50
BsiHKAI G_wGCw'C
Cuts at: 2428 2435 2745 2758 2943 3297 3561 4725 4810
Size: 7 310 13 185 354 264 1164 85
Cuts at: 4810 5881 2428
Size: 1071 3211
Fragments arranged by size:
3211 1164 1071 354 310 264 185 85
13 7
BsmI GAATG_Cn'
Cuts at: 4175 4268 4175
Size: 93 6571
BsmBI CGTCTCn'nnnn_
Cuts at: 3692 3692
Size: 6664
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2428 2435 2518 2745 2758 2943 3297
Size: 399 638 7 83 227 13 185 354
Cuts at: 3297 3347 3561 3595 3800 3860 3960 3983 4725
Size: 50 214 34 205 60 100 23 742
Cuts at: 4725 4810 5881 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 638 399 354 227 214
205 185 100 85 83 60 50 34
23 13 7
BspEI T'CCGG_A
Cuts at: 6327 6327
Size: 6664
BspHI T'CATG_A
Cuts at: 4553 6283 4553
Size: 1730 4934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2505 2730 3258 4019 844
Size: 1661 225 528 761 3489
Fragments arranged by size:
3489 1661 761 528 225
BsrBI CCG'CTC
Cuts at: 1394 1823 2097 3350 3589 4551 1394
Size: 429 274 1253 239 962 3507
Fragments arranged by size:
3507 1253 962 429 274 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 2458 5159 5333 65
Size: 1183 1210 2701 174 1396
Fragments arranged by size:
2701 1396 1210 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2114 3383 3440 3896 5308 5495
Size: 110 198 106 1269 57 456 1412 187
Cuts at: 5495 1700
Size: 2869
Fragments arranged by size:
2869 1412 1269 456 198 187 110 106
57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6664
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6664
BssSI C'ACGA_G
Cuts at: 3554 4724 5736 3554
Size: 1170 1012 4482
Fragments arranged by size:
4482 1170 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2086 2809 3711 794
Size: 1292 723 902 3747
Fragments arranged by size:
3747 1292 902 723
BstBI TT'CG_AA
Cuts at: 5468 5468
Size: 6664
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6664
BstXI CCAn_nnnn'nTGG
Cuts at: 3779 3808 3779
Size: 29 6635
BstYI r'GATC_y
Cuts at: 1972 2051 3994 4361 4746 4763 6215 6301 6313
Size: 79 1943 367 385 17 1452 86 12
Cuts at: 6313 6659 1972
Size: 346 1977
Fragments arranged by size:
1977 1943 1452 385 367 346 86 79
17 12
Bsu36I CC'TnA_GG
Cuts at: 3453 3453
Size: 6664
BtgI C'CryG_G
Cuts at: 513 1065 2338 3048 3357 3567 3807 513
Size: 552 1273 710 309 210 240 3370
Fragments arranged by size:
3370 1273 710 552 309 240 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2201 487
Size: 683 56 975 4950
Fragments arranged by size:
4950 975 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 4180 4974 4994 2552
Size: 1628 794 20 4222
Fragments arranged by size:
4222 1628 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6629
DraI TTT'AAA
Cuts at: 3968 3968
Size: 6664
DraIII CAC_nnn'GTG
Cuts at: 2086 3775 2086
Size: 1689 4975
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5671 809
Size: 507 4355 1802
Fragments arranged by size:
4355 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2341 2460 2690
Size: 54 1425 50 275 18 511 119 230
Cuts at: 2690 2763 2954 3113 3597 3781 3813 4111 5000
Size: 73 191 159 484 184 32 298 889
Cuts at: 5000 6629 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 511 484 298 275 230
191 184 159 119 73 54 50 43
32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2341 2690 3113 4111 1537
Size: 275 529 349 423 998 4090
Fragments arranged by size:
4090 998 529 423 349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3180 3471 4594 1353
Size: 1827 291 1123 3423
Fragments arranged by size:
3423 1827 1123 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3370 3700 5251 5625 5771 387
Size: 1064 138 1781 330 1551 374 146 1280
Fragments arranged by size:
1781 1551 1280 1064 374 330 146 138
EcoICRI GAG'CTC
Cuts at: 2743 3295 3559 2743
Size: 552 264 5848
Fragments arranged by size:
5848 552 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2431 2648 2732 3056 3484 3495
Size: 393 479 320 217 84 324 428 11
Cuts at: 3495 3643 4727 5324 6111 6379 1239
Size: 148 1084 597 787 268 1524
Fragments arranged by size:
1524 1084 787 597 479 428 393 324
320 268 217 148 84 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3599 3599
Size: 6664
EcoO109I rG'GnC_Cy
Cuts at: 2135 2514 3132 3343 3344 3395 3432 3592 3735
Size: 379 618 211 1 51 37 160 143
Cuts at: 3735 3796 3797 3857 3908 2135
Size: 61 1 60 51 4891
Fragments arranged by size:
4891 618 379 211 160 143 61 60
51 51 37 1 1
EcoRI G'AATT_C
Cuts at: 3973 4351 3973
Size: 378 6286
FspI TGC'GCA
Cuts at: 2603 3266 5170 2603
Size: 663 1904 4097
Fragments arranged by size:
4097 1904 663
FspAI rTGC'GCAy
Cuts at: 2603 2603
Size: 6664
HaeII r_GCGC'y
Cuts at: 1678 2542 2826 2983 3109 3250 3316 5811 1678
Size: 864 284 157 126 141 66 2495 2531
Fragments arranged by size:
2531 2495 864 284 157 141 126 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2897 2929 5305 5337 5379
Size: 32 135 32 1325 32 2376 32 42
Cuts at: 5379 5411 1373
Size: 32 2626
Fragments arranged by size:
2626 2376 1325 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2866 4008 4254 669
Size: 988 281 928 1142 246 3079
Fragments arranged by size:
3079 1142 988 928 281 246
HindIII A'AGCT_T
Cuts at: 748 2660 748
Size: 1912 4752
HpaI GTT'AAC
Cuts at: 4254 4254
Size: 6664
KpnI G_GTAC'C
Cuts at: 3989 3989
Size: 6664
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3492 3513 5778 5962 1305
Size: 2187 21 2265 184 2007
Fragments arranged by size:
2265 2187 2007 184 21
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2765 3783 10
Size: 54 1425 343 933 1018 2891
Fragments arranged by size:
2891 1425 1018 933 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 3047 3227 3777 3806 4622 4981
Size: 648 1443 438 180 550 29 816 359
Cuts at: 4981 5140 518
Size: 159 2042
Fragments arranged by size:
2042 1443 816 648 550 438 359 180
159 29
MspA1I CmG'CkG
Cuts at: 1606 2204 2737 2929 3359 3708 4080 4757 5905
Size: 598 533 192 430 349 372 677 1148
Cuts at: 5905 6150 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 598 533 430 372 349
245 192
NaeI GCC'GGC
Cuts at: 2010 2116 3898 2010
Size: 106 1782 4776
Fragments arranged by size:
4776 1782 106
NcoI C'CATG_G
Cuts at: 513 1065 3048 513
Size: 552 1983 4129
Fragments arranged by size:
4129 1983 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6664
NgoMIV G'CCGG_C
Cuts at: 2008 2114 3896 2008
Size: 106 1782 4776
Fragments arranged by size:
4776 1782 106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6664
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NruI TCG'CGA
Cuts at: 3417 3417
Size: 6664
NspI r_CATG'y
Cuts at: 1132 1229 2245 4022 5567 1132
Size: 97 1016 1777 1545 2229
Fragments arranged by size:
2229 1777 1545 1016 97
PasI CC'CwG_GG
Cuts at: 1348 2269 1348
Size: 921 5743
PciI A'CATG_T
Cuts at: 5563 5563
Size: 6664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2588 2788 3853 1108
Size: 1480 200 1065 3919
Fragments arranged by size:
3919 1480 1065 200
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PmlI CAC'GTG
Cuts at: 3775 3775
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 6246 6278 4733
Size: 32 1481 32 5119
Fragments arranged by size:
5119 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2135 3132 3432 3735 2135
Size: 997 300 303 5064
Fragments arranged by size:
5064 997 303 300
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6664
PsiI TTA'TAA
Cuts at: 4234 4234
Size: 6664
PspOMI G'GGCC_C
Cuts at: 2514 3343 3591 3796 3856 2514
Size: 829 248 205 60 5322
Fragments arranged by size:
5322 829 248 205 60
PstI C_TGCA'G
Cuts at: 830 2559 2736 3522 3795 4016 830
Size: 1729 177 786 273 221 3478
Fragments arranged by size:
3478 1729 786 273 221 177
PvuI CG_AT'CG
Cuts at: 2001 2643 2001
Size: 642 6022
SacI G_AGCT'C
Cuts at: 2745 3297 3561 2745
Size: 552 264 5848
Fragments arranged by size:
5848 552 264
SacII CC_GC'GG
Cuts at: 3360 3360
Size: 6664
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SanDI GG'GwC_CC
Cuts at: 2135 3132 2135
Size: 997 5667
SbfI CC_TGCA'GG
Cuts at: 3795 4016 3795
Size: 221 6443
ScaI AGT'ACT
Cuts at: 2786 4912 6624 2786
Size: 2126 1712 2826
Fragments arranged by size:
2826 2126 1712
SfcI C'TryA_G
Cuts at: 826 1045 2555 2732 2983 3316 3518 3791 4012
Size: 219 1510 177 251 333 202 273 221
Cuts at: 4012 5147 5828 6019 826
Size: 1135 681 191 1471
Fragments arranged by size:
1510 1471 1135 681 333 273 251 221
219 202 191 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 2132 3092 3991 1872
Size: 260 960 899 4545
Fragments arranged by size:
4545 960 899 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2534 4768 5669 5931 820
Size: 197 926 591 2234 901 262 1553
Fragments arranged by size:
2234 1553 926 901 591 262 197
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 2245 4022 1132
Size: 1113 1777 3774
Fragments arranged by size:
3774 1777 1113
SspI AAT'ATT
Cuts at: 5 52 4588 5
Size: 47 4536 2081
Fragments arranged by size:
4536 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 3617 1517
Size: 663 1437 4564
Fragments arranged by size:
4564 1437 663
StyI C'CwwG_G
Cuts at: 513 1065 3048 4103 513
Size: 552 1983 1055 3074
Fragments arranged by size:
3074 1983 1055 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2452 3096 3863 4691 4708 4861 5046
Size: 1255 872 644 767 828 17 153 185
Cuts at: 5046 5491 325
Size: 445 1498
Fragments arranged by size:
1498 1255 872 828 767 644 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 4910 6622
Size: 274 80 33 51 1443 807 2126 1712
Cuts at: 6622 96
Size: 138
Fragments arranged by size:
2126 1712 1443 807 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3679 3711 6624 6656 3679
Size: 32 2913 32 3687
Fragments arranged by size:
3687 2913 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6664
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3808 3808
Size: 6664
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6664
XmaI C'CCGG_G
Cuts at: 1870 2130 3090 3989 1870
Size: 260 960 899 4545
Fragments arranged by size:
4545 960 899 260
XmnI GAAnn'nnTTC
Cuts at: 4793 4793
Size: 6664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI KasI NarI NsiI PacI
PfoI PspXI PsrI PvuII RsrII SapI SexAI SfiI
SfoI SrfI SwaI