Restriction map for
pFN21AB6174
(Circular) (Six-base) MAPSORT of: pFN21AB6174.seq Check: 815 from: 1 to: 6595
pFN21AB6174 6595 bp
With 182 enzymes: SgfI *
May 6, 2010 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2591 3025 3398 3472 2591
Size: 434 373 74 5714
Fragments arranged by size:
5714 434 373 74
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4405 278
Size: 53 83 186 936 2869 2468
Fragments arranged by size:
2869 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3042 3669 3938 1937
Size: 1105 627 269 4594
Fragments arranged by size:
4594 1105 627 269
Acc65I G'GTAC_C
Cuts at: 3916 3916
Size: 6595
AclI AA'CG_TT
Cuts at: 1563 2459 4722 5095 1563
Size: 896 2263 373 3063
Fragments arranged by size:
3063 2263 896 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4658 6042 6310 1632
Size: 3026 1384 268 1917
Fragments arranged by size:
3026 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6398
AflIII A'CryG_T
Cuts at: 1203 5494 6471 1203
Size: 4291 977 1327
Fragments arranged by size:
4291 1327 977
AgeI A'CCGG_T
Cuts at: 5426 5426
Size: 6595
AhdI GACnn_n'nnGTC
Cuts at: 5324 5324
Size: 6595
AleI CACnn'nnGTG
Cuts at: 1166 3791 1166
Size: 2625 3970
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3494 1184
Size: 2310 4285
AlwNI CAG_nnn'CTG
Cuts at: 2944 3246 3257 4338 5910 2944
Size: 302 11 1081 1572 3629
Fragments arranged by size:
3629 1572 1081 302 11
ApaI G_GGCC'C
Cuts at: 2042 2168 3494 2042
Size: 126 1326 5143
Fragments arranged by size:
5143 1326 126
ApaLI G'TGCA_C
Cuts at: 4652 5808 4652
Size: 1156 5439
ApoI r'AATT_y
Cuts at: 1493 3161 3904 4130 4282 6391 1493
Size: 1668 743 226 152 2109 1697
Fragments arranged by size:
2109 1697 1668 743 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5149 160
Size: 872 4117 1606
Fragments arranged by size:
4117 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
AvaI C'yCGr_G
Cuts at: 1870 1943 2338 3920 1870
Size: 73 395 1582 4545
Fragments arranged by size:
4545 1582 395 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3828 3861 3828
Size: 33 6562
BamHI G'GATC_C
Cuts at: 2130 3925 4292 2130
Size: 1795 367 4433
Fragments arranged by size:
4433 1795 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2435 2760 2796 2925 3201
Size: 325 217 627 648 325 36 129 276
Cuts at: 3201 3321 3484 3643 3916 5371 618
Size: 120 163 159 273 1455 1842
Fragments arranged by size:
1842 1455 648 627 325 325 276 273
217 163 159 129 120 36
BanII G_rGCy'C
Cuts at: 1391 2042 2117 2168 2938 2979 3001 3494 3914
Size: 651 75 51 770 41 22 493 420
Cuts at: 3914 1391
Size: 4072
Fragments arranged by size:
4072 770 651 493 420 75 51 41
22
BbeI G_GCGC'C
Cuts at: 2764 2929 3205 2764
Size: 165 276 6154
Fragments arranged by size:
6154 276 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2568 928
Size: 971 669 4955
Fragments arranged by size:
4955 971 669
BbvCI CC'TCA_GC
Cuts at: 2010 2010
Size: 6595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4786 4820 1903
Size: 34 2849 34 3678
Fragments arranged by size:
3678 2849 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3583 4487 5697 3583
Size: 904 1210 4481
Fragments arranged by size:
4481 1210 904
BclI T'GATC_A
Cuts at: 1548 3795 1548
Size: 2247 4348
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2228 2275 5206
Size: 107 122 71 631 605 556 47 2931
Cuts at: 5206 136
Size: 1525
Fragments arranged by size:
2931 1525 631 605 556 122 107 71
47
BglII A'GATC_T
Cuts at: 6590 6590
Size: 6595
BlpI GC'TnA_GC
Cuts at: 4012 4012
Size: 6595
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2168 3494 4656 5812 1790
Size: 252 126 1326 1162 1156 2573
Fragments arranged by size:
2573 1326 1162 1156 252 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6595
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3234 3526 4326
Size: 416 53 83 399 24 1817 292 800
Cuts at: 4326 5284 442
Size: 958 1753
Fragments arranged by size:
1817 1753 958 800 416 399 292 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3900 5255 1239
Size: 2661 1355 2579
Fragments arranged by size:
2661 2579 1355
Bpu10I CC'TnA_GC
Cuts at: 2010 2010
Size: 6595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4720 5585 5883 4720
Size: 865 298 5432
Fragments arranged by size:
5432 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2342 2759 5258 882
Size: 617 843 417 2499 2219
Fragments arranged by size:
2499 2219 843 617 417
BsaAI yAC'GTr
Cuts at: 493 1204 4386 493
Size: 711 3182 2702
Fragments arranged by size:
3182 2702 711
BsaBI GATnn'nnATC
Cuts at: 6589 6589
Size: 6595
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2761 2926 3202 4402
Size: 53 83 186 936 1228 165 276 1200
Cuts at: 4402 4784 5419 275
Size: 382 635 1451
Fragments arranged by size:
1451 1228 1200 936 635 382 276 186
165 83 53
BsaWI w'CCGG_w
Cuts at: 2652 5028 5426 5700 5847 6258 2652
Size: 2376 398 274 147 411 2989
Fragments arranged by size:
2989 2376 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2634 2664 3373 3403 3518
Size: 30 165 30 1231 30 709 30 115
Cuts at: 3518 3548 1178
Size: 30 4225
Fragments arranged by size:
4225 1231 709 165 115 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3728 3740 1186
Size: 2542 12 4041
Fragments arranged by size:
4041 2542 12
BseYI C'CCAG_C
Cuts at: 2374 2937 3305 5798 6294 2374
Size: 563 368 2493 496 2675
Fragments arranged by size:
2675 2493 563 496 368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2562 3123 3500 1163
Size: 573 826 561 377 4258
Fragments arranged by size:
4258 826 573 561 377
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4045 4806 5834 1540
Size: 275 186 2044 761 1028 2301
Fragments arranged by size:
2301 2044 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2482 3539 4656 4741 5812 2482
Size: 1057 1117 85 1071 3265
Fragments arranged by size:
3265 1117 1071 1057 85
BsmI GAATG_Cn'
Cuts at: 4106 4199 4106
Size: 93 6502
BsmBI CGTCTCn'nnnn_
Cuts at: 2635 2635
Size: 6595
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2117 2168 2482 2938 2979 3001
Size: 399 252 75 51 314 456 41 22
Cuts at: 3001 3494 3539 3914 4656 4741 5812 1391
Size: 493 45 375 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 456 399 375 314
252 85 75 51 45 41 22
BspEI T'CCGG_A
Cuts at: 2652 6258 2652
Size: 3606 2989
BspHI T'CATG_A
Cuts at: 4484 6214 4484
Size: 1730 4865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2591 3025 3398 3472 3883 3950 844
Size: 1747 434 373 74 411 67 3489
Fragments arranged by size:
3489 1747 434 411 373 74 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4482 1394
Size: 429 2659 3507
Fragments arranged by size:
3507 2659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2719 5090 5264 65
Size: 1183 1471 2371 174 1396
Fragments arranged by size:
2371 1471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3075 5239 5426 1700
Size: 110 1265 2164 187 2869
Fragments arranged by size:
2869 2164 1265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6595
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6595
BssSI C'ACGA_G
Cuts at: 4655 5667 4655
Size: 1012 5583
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2211 794
Size: 1417 5178
BstBI TT'CG_AA
Cuts at: 5399 5399
Size: 6595
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6595
BstXI CCAn_nnnn'nTGG
Cuts at: 3001 3539 3001
Size: 538 6057
BstYI r'GATC_y
Cuts at: 1972 2130 3925 4292 4677 4694 6146 6232 6244
Size: 158 1795 367 385 17 1452 86 12
Cuts at: 6244 6590 1972
Size: 346 1977
Fragments arranged by size:
1977 1795 1452 385 367 346 158 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2238 513
Size: 552 939 234 4870
Fragments arranged by size:
4870 939 552 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5856
Fragments arranged by size:
5856 683 56
BtsI GCAGTG_nn'
Cuts at: 2942 4111 4905 4925 2942
Size: 1169 794 20 4612
Fragments arranged by size:
4612 1169 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6560
DraI TTT'AAA
Cuts at: 3899 3899
Size: 6595
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5602 809
Size: 507 4286 1802
Fragments arranged by size:
4286 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2716 3130 4042
Size: 54 1425 50 275 18 886 414 912
Cuts at: 4042 4931 6560 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 912 889 886 414 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4042 1537
Size: 275 2230 4090
Fragments arranged by size:
4090 2230 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2192 3762 4525 1353
Size: 839 1570 763 3423
Fragments arranged by size:
3423 1570 839 763
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3133 3602 5182 5556 5702 387
Size: 1064 138 1544 469 1580 374 146 1280
Fragments arranged by size:
1580 1544 1280 1064 469 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3900 4658 5255 6042 6310 1239
Size: 393 2268 758 597 787 268 1524
Fragments arranged by size:
2268 1524 787 758 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3521 3521
Size: 6595
EcoO109I rG'GnC_Cy
Cuts at: 2039 2165 2335 2571 3096 3491 2039
Size: 126 170 236 525 395 5143
Fragments arranged by size:
5143 525 395 236 170 126
EcoRI G'AATT_C
Cuts at: 3904 4282 3904
Size: 378 6217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3367 3367
Size: 6595
FspI TGC'GCA
Cuts at: 5101 5101
Size: 6595
HaeII r_GCGC'y
Cuts at: 1678 2056 2764 2929 3187 3205 5742 1678
Size: 378 708 165 258 18 2537 2531
Fragments arranged by size:
2537 2531 708 378 258 165 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 2597 2629 2634
Size: 32 135 32 543 32 450 32 5
Cuts at: 2634 2666 3731 3763 5236 5268 5310 5342 1373
Size: 32 1065 32 1473 32 42 32 2626
Fragments arranged by size:
2626 1473 1065 543 450 135 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3939 4185 669
Size: 988 281 2001 246 3079
Fragments arranged by size:
3079 2001 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6595
HpaI GTT'AAC
Cuts at: 4185 4185
Size: 6595
KasI G'GCGC_C
Cuts at: 2760 2925 3201 2760
Size: 165 276 6154
Fragments arranged by size:
6154 276 165
KpnI G_GTAC'C
Cuts at: 3920 3920
Size: 6595
MfeI C'AATT_G
Cuts at: 4194 4194
Size: 6595
MluI A'CGCG_T
Cuts at: 6471 6471
Size: 6595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2284 3416 5709 5893 1305
Size: 979 1132 2293 184 2007
Fragments arranged by size:
2293 2007 1132 979 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2718 10
Size: 54 1425 343 886 3887
Fragments arranged by size:
3887 1425 886 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3791 4553 4912 5071 518
Size: 648 2625 762 359 159 2042
Fragments arranged by size:
2625 2042 762 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2449 2596 3135 3620 4011 4688 5836 6081
Size: 843 147 539 485 391 677 1148 245
Cuts at: 6081 1606
Size: 2120
Fragments arranged by size:
2120 1148 843 677 539 485 391 245
147
NaeI GCC'GGC
Cuts at: 3077 3077
Size: 6595
NarI GG'CG_CC
Cuts at: 2761 2926 3202 2761
Size: 165 276 6154
Fragments arranged by size:
6154 276 165
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5104
Fragments arranged by size:
5104 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6595
NgoMIV G'CCGG_C
Cuts at: 3075 3075
Size: 6595
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6595
NotI GC'GGCC_GC
Cuts at: 4042 4042
Size: 6595
NspI r_CATG'y
Cuts at: 1132 1229 2882 3953 5498 1132
Size: 97 1653 1071 1545 2229
Fragments arranged by size:
2229 1653 1545 1071 97
PasI CC'CwG_GG
Cuts at: 1348 2035 1348
Size: 687 5908
PciI A'CATG_T
Cuts at: 5494 5494
Size: 6595
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 2608 3246 1108
Size: 1131 369 638 4457
Fragments arranged by size:
4457 1131 638 369
PmeI GTTT'AAAC
Cuts at: 3899 3899
Size: 6595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4664 4696 6177 6209 4664
Size: 32 1481 32 5050
Fragments arranged by size:
5050 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6595
PsiI TTA'TAA
Cuts at: 4165 4165
Size: 6595
PspOMI G'GGCC_C
Cuts at: 2038 2164 3490 2038
Size: 126 1326 5143
Fragments arranged by size:
5143 1326 126
PstI C_TGCA'G
Cuts at: 830 2595 3216 3947 830
Size: 1765 621 731 3478
Fragments arranged by size:
3478 1765 731 621
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6595
PvuII CAG'CTG
Cuts at: 2596 3620 2596
Size: 1024 5571
SalI G'TCGA_C
Cuts at: 1936 3937 1936
Size: 2001 4594
SbfI CC_TGCA'GG
Cuts at: 3947 3947
Size: 6595
ScaI AGT'ACT
Cuts at: 4843 6555 4843
Size: 1712 4883
SfcI C'TryA_G
Cuts at: 826 1045 2591 3056 3212 3943 5078 5759 5950
Size: 219 1546 465 156 731 1135 681 191
Cuts at: 5950 826
Size: 1471
Fragments arranged by size:
1546 1471 1135 731 681 465 219 191
156
SfoI GGC'GCC
Cuts at: 2762 2927 3203 2762
Size: 165 276 6154
Fragments arranged by size:
6154 276 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6595
SmaI CCC'GGG
Cuts at: 1872 3922 1872
Size: 2050 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4699 5600 5862 820
Size: 197 926 2756 901 262 1553
Fragments arranged by size:
2756 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4386 493
Size: 3893 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6595
SphI G_CATG'C
Cuts at: 1132 3953 1132
Size: 2821 3774
SspI AAT'ATT
Cuts at: 5 52 4519 5
Size: 47 4467 2081
Fragments arranged by size:
4467 2081 47
StuI AGG'CCT
Cuts at: 1517 3678 1517
Size: 2161 4434
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 2352 3351 3854 4034 513
Size: 552 939 222 126 999 503 180 3074
Fragments arranged by size:
3074 999 939 552 503 222 180 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2385 2453 3806 4622 4639 4792 4977
Size: 1255 805 68 1353 816 17 153 185
Cuts at: 4977 5422 325
Size: 445 1498
Fragments arranged by size:
1498 1353 1255 816 805 445 185 153
68 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4841 6553 96
Size: 274 80 33 51 1443 2864 1712 138
Fragments arranged by size:
2864 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3112 3144 3518 3550 6555 6587 3112
Size: 32 374 32 3005 32 3120
Fragments arranged by size:
3120 3005 374 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6595
XbaI T'CTAG_A
Cuts at: 3931 3931
Size: 6595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2711 2711
Size: 6595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6595
XmaI C'CCGG_G
Cuts at: 1870 3920 1870
Size: 2050 4545
XmnI GAAnn'nnTTC
Cuts at: 2064 4724 2064
Size: 2660 3935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4403 276
Size: 53 83 186 936 2869 2468
Fragments arranged by size:
2869 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI