Restriction map for
pFN21AB6173
(Circular) (Six-base) MAPSORT of: pFN21AB6173.seq Check: 5577 from: 1 to: 6664
pFN21AB6173 6664 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2786 2786
Size: 6664
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4007 1937
Size: 2070 4594
Acc65I G'GTAC_C
Cuts at: 3985 3985
Size: 6664
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2424 2875 3451 4727 6111 6379 1632
Size: 792 451 576 1276 1384 268 1917
Fragments arranged by size:
1917 1384 1276 792 576 451 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6467
AflIII A'CryG_T
Cuts at: 1203 3397 5563 6540 1203
Size: 2194 2166 977 1327
Fragments arranged by size:
2194 2166 1327 977
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2838 2848 1184
Size: 1654 10 5000
Fragments arranged by size:
5000 1654 10
AlwNI CAG_nnn'CTG
Cuts at: 2605 3748 4407 5979 2605
Size: 1143 659 1572 3290
Fragments arranged by size:
3290 1572 1143 659
ApaI G_GGCC'C
Cuts at: 2921 2921
Size: 6664
ApaLI G'TGCA_C
Cuts at: 2637 4721 5877 2637
Size: 2084 1156 3424
Fragments arranged by size:
3424 2084 1156
ApoI r'AATT_y
Cuts at: 1493 2238 3022 3333 3405 3501 3973 4199 4351
Size: 745 784 311 72 96 472 226 152
Cuts at: 4351 6460 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 784 745 472 311 226 152
96 72
AseI AT'TA_AT
Cuts at: 160 1032 5218 160
Size: 872 4186 1606
Fragments arranged by size:
4186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 2786 3989 1870
Size: 73 586 257 1203 4545
Fragments arranged by size:
4545 1203 586 257 73
AvrII C'CTAG_G
Cuts at: 2632 2632
Size: 6664
BamHI G'GATC_C
Cuts at: 2293 3994 4361 2293
Size: 1701 367 4596
Fragments arranged by size:
4596 1701 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2771 2967 3912 3985 5440
Size: 325 217 627 984 196 945 73 1455
Cuts at: 5440 618
Size: 1842
Fragments arranged by size:
1842 1455 984 945 627 325 217 196
73
BanII G_rGCy'C
Cuts at: 1391 2274 2921 3647 3815 3899 3983 1391
Size: 883 647 726 168 84 84 4072
Fragments arranged by size:
4072 883 726 647 168 84 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5693
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2777 2811 4855 4889 1903
Size: 34 840 34 2044 34 3678
Fragments arranged by size:
3678 2044 840 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2274 4556 5766 2274
Size: 2282 1210 3172
Fragments arranged by size:
3172 2282 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6664
BfrBI ATG'CAT
Cuts at: 3863 3863
Size: 6664
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5275 136
Size: 107 122 71 631 605 3603 1525
Fragments arranged by size:
3603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6659 6659
Size: 6664
BlpI GC'TnA_GC
Cuts at: 3730 3768 4081 3730
Size: 38 313 6313
Fragments arranged by size:
6313 313 38
Bme1580I G_kGCm'C
Cuts at: 1790 2641 2774 2921 2970 3915 4725 5881 1790
Size: 851 133 147 49 945 810 1156 2573
Fragments arranged by size:
2573 1156 945 851 810 147 133 49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6664
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 2427 2831
Size: 416 53 83 399 24 658 352 404
Cuts at: 2831 4395 5353 442
Size: 1564 958 1753
Fragments arranged by size:
1753 1564 958 658 416 404 399 352
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1996 2156 2258 2627 2642 2975 3245 3699
Size: 757 160 102 369 15 333 270 454
Cuts at: 3699 5324 1239
Size: 1625 2579
Fragments arranged by size:
2579 1625 757 454 369 333 270 160
102 15
Bpu10I CC'TnA_GC
Cuts at: 2268 3171 2268
Size: 903 5761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2229 3061 4789 5654 5952 2229
Size: 832 1728 865 298 2941
Fragments arranged by size:
2941 1728 865 832 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 2951 2980 3679 5327 882
Size: 617 662 790 29 699 1648 2219
Fragments arranged by size:
2219 1648 790 699 662 617 29
BsaAI yAC'GTr
Cuts at: 493 1204 3398 4455 493
Size: 711 2194 1057 2702
Fragments arranged by size:
2702 2194 1057 711
BsaBI GATnn'nnATC
Cuts at: 6658 6658
Size: 6664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4471 4853 5488 275
Size: 53 83 186 936 2938 382 635 1451
Fragments arranged by size:
2938 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2655 3552 3593 5097 5495 5769 5916 6327 2655
Size: 897 41 1504 398 274 147 411 2992
Fragments arranged by size:
2992 1504 897 411 398 274 147 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6439
Fragments arranged by size:
6439 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2750 2822 2870 2915 1186
Size: 1033 531 72 48 45 4935
Fragments arranged by size:
4935 1033 531 72 48 45
BseYI C'CCAG_C
Cuts at: 2799 2914 3053 3741 5867 6363 2799
Size: 115 139 688 2126 496 3100
Fragments arranged by size:
3100 2126 688 496 139 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 1163
Size: 573 706 5385
Fragments arranged by size:
5385 706 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4114 4875 5903 1540
Size: 275 186 2113 761 1028 2301
Fragments arranged by size:
2301 2113 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 2641 2850 3238 3647 3815 3899 4725 4810
Size: 367 209 388 409 168 84 826 85
Cuts at: 4810 5881 2274
Size: 1071 3057
Fragments arranged by size:
3057 1071 826 409 388 367 209 168
85 84
BsmI GAATG_Cn'
Cuts at: 2383 4175 4268 2383
Size: 1792 93 4779
Fragments arranged by size:
4779 1792 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 2641 2774 2850 2921 2970 3238
Size: 399 484 367 133 76 71 49 268
Cuts at: 3238 3647 3815 3899 3915 3983 4725 4810 5881
Size: 409 168 84 16 68 742 85 1071
Cuts at: 5881 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 484 409 399 367 268
168 133 85 84 76 71 68 49
16
BspEI T'CCGG_A
Cuts at: 2655 3552 6327 2655
Size: 897 2775 2992
Fragments arranged by size:
2992 2775 897
BspHI T'CATG_A
Cuts at: 4553 6283 4553
Size: 1730 4934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2431 2770 2786 4019 844
Size: 1587 339 16 1233 3489
Fragments arranged by size:
3489 1587 1233 339 16
BsrBI CCG'CTC
Cuts at: 1394 1823 4551 1394
Size: 429 2728 3507
Fragments arranged by size:
3507 2728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3454 5159 5333 65
Size: 1183 2206 1705 174 1396
Fragments arranged by size:
2206 1705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5308 5495 1700
Size: 110 3498 187 2869
Fragments arranged by size:
3498 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2118 96
Size: 2022 4642
BssHII G'CGCG_C
Cuts at: 1925 2483 1925
Size: 558 6106
BssSI C'ACGA_G
Cuts at: 4724 5736 4724
Size: 1012 5652
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2778 794
Size: 1984 4680
BstBI TT'CG_AA
Cuts at: 5468 5468
Size: 6664
BstEII G'GTnAC_C
Cuts at: 1184 2468 1184
Size: 1284 5380
BstYI r'GATC_y
Cuts at: 1972 2293 2385 2601 3994 4361 4746 4763 6215
Size: 321 92 216 1393 367 385 17 1452
Cuts at: 6215 6301 6313 6659 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1393 385 367 346 321 216
92 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2475 2580 2853 3036 3749 2475
Size: 105 273 183 713 5390
Fragments arranged by size:
5390 713 273 183 105
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5173
Fragments arranged by size:
5173 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2958 487
Size: 683 56 1732 4193
Fragments arranged by size:
4193 1732 683 56
BtsI GCAGTG_nn'
Cuts at: 2639 2793 3538 4180 4974 4994 2639
Size: 154 745 642 794 20 4309
Fragments arranged by size:
4309 794 745 642 154 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3856 3891 529
Size: 35 3292 35 3302
Fragments arranged by size:
3302 3292 35 35
DraI TTT'AAA
Cuts at: 3968 3968
Size: 6664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2029 2728 5671 809
Size: 507 713 699 2943 1802
Fragments arranged by size:
2943 1802 713 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2222 4111 5000
Size: 54 1425 50 275 18 392 1889 889
Cuts at: 5000 6629 8
Size: 1629 43
Fragments arranged by size:
1889 1629 1425 889 392 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4111 1537
Size: 275 2299 4090
Fragments arranged by size:
4090 2299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2304 3410 3652 4594 1353
Size: 723 228 1106 242 942 3423
Fragments arranged by size:
3423 1106 942 723 242 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3794 5251 5625 5771 387
Size: 1064 138 2205 1457 374 146 1280
Fragments arranged by size:
2205 1457 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2272 3645 3813 3897 2272
Size: 1373 168 84 5039
Fragments arranged by size:
5039 1373 168 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1996 2156 2258 2424 2627 2642 2875
Size: 393 364 160 102 166 203 15 233
Cuts at: 2875 2975 3245 3451 3699 4727 5324 6111 6379
Size: 100 270 206 248 1028 597 787 268
Cuts at: 6379 1239
Size: 1524
Fragments arranged by size:
1524 1028 787 597 393 364 270 268
248 233 206 203 166 160 102 100
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2711 2711
Size: 6664
EcoO109I rG'GnC_Cy
Cuts at: 2341 2392 2525 2536 3603 3714 2341
Size: 51 133 11 1067 111 5291
Fragments arranged by size:
5291 1067 133 111 51 11
EcoRI G'AATT_C
Cuts at: 2238 3501 3973 4351 2238
Size: 1263 472 378 4551
Fragments arranged by size:
4551 1263 472 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 6664
FspI TGC'GCA
Cuts at: 3356 5170 3356
Size: 1814 4850
HaeII r_GCGC'y
Cuts at: 1678 3303 3768 3856 5811 1678
Size: 1625 465 88 1955 2531
Fragments arranged by size:
2531 1955 1625 465 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 5305 5337 5379
Size: 32 135 32 605 32 3096 32 42
Cuts at: 5379 5411 1373
Size: 32 2626
Fragments arranged by size:
3096 2626 605 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4008 4254 669
Size: 988 281 2070 246 3079
Fragments arranged by size:
3079 2070 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6664
HpaI GTT'AAC
Cuts at: 4254 4254
Size: 6664
KpnI G_GTAC'C
Cuts at: 3989 3989
Size: 6664
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2045 2470 3452 5778 5962 1305
Size: 740 425 982 2326 184 2007
Fragments arranged by size:
2326 2007 982 740 425 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2224 10
Size: 54 1425 343 392 4450
Fragments arranged by size:
4450 1425 392 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2466 3694 3862 4622 4981 5140 518
Size: 648 1300 1228 168 760 359 159 2042
Fragments arranged by size:
2042 1300 1228 760 648 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 2278 4080 4757 5905 6150 1606
Size: 672 1802 677 1148 245 2120
Fragments arranged by size:
2120 1802 1148 677 672 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5173
Fragments arranged by size:
5173 939 552
NdeI CA'TA_TG
Cuts at: 387 3859 387
Size: 3472 3192
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6664
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NsiI A_TGCA'T
Cuts at: 3865 3865
Size: 6664
NspI r_CATG'y
Cuts at: 1132 1229 3867 4022 5567 1132
Size: 97 2638 155 1545 2229
Fragments arranged by size:
2638 2229 1545 155 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6664
PciI A'CATG_T
Cuts at: 5563 5563
Size: 6664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 2698 2878 1108
Size: 1254 336 180 4894
Fragments arranged by size:
4894 1254 336 180
PfoI T'CCnGG_A
Cuts at: 2604 2871 3129 2604
Size: 267 258 6139
Fragments arranged by size:
6139 267 258
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PmlI CAC'GTG
Cuts at: 3398 3398
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 4733 4765 6246 6278 2141
Size: 32 2560 32 1481 32 2527
Fragments arranged by size:
2560 2527 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 2525 3714 2341
Size: 184 1189 5291
Fragments arranged by size:
5291 1189 184
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6664
PsiI TTA'TAA
Cuts at: 2511 3497 4234 2511
Size: 986 737 4941
Fragments arranged by size:
4941 986 737
PspOMI G'GGCC_C
Cuts at: 2917 2917
Size: 6664
PspXI vC'TCGA_Gb
Cuts at: 2786 2786
Size: 6664
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 6632
PstI C_TGCA'G
Cuts at: 830 2755 4016 830
Size: 1925 1261 3478
Fragments arranged by size:
3478 1925 1261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
PvuII CAG'CTG
Cuts at: 2278 2278
Size: 6664
SacI G_AGCT'C
Cuts at: 2274 3647 3815 3899 2274
Size: 1373 168 84 5039
Fragments arranged by size:
5039 1373 168 84
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SapI GCTCTTCn'nnn_
Cuts at: 3410 3652 3410
Size: 242 6422
SbfI CC_TGCA'GG
Cuts at: 2755 4016 2755
Size: 1261 5403
ScaI AGT'ACT
Cuts at: 2745 3478 4912 6624 2745
Size: 733 1434 1712 2785
Fragments arranged by size:
2785 1712 1434 733
SexAI A'CCwGG_T
Cuts at: 2151 2970 2151
Size: 819 5845
SfcI C'TryA_G
Cuts at: 826 1045 2143 2573 2751 4012 5147 5828 6019
Size: 219 1098 430 178 1261 1135 681 191
Cuts at: 6019 826
Size: 1471
Fragments arranged by size:
1471 1261 1135 1098 681 430 219 191
178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 2531 3991 1872
Size: 659 1460 4545
Fragments arranged by size:
4545 1460 659
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2244 2786 3076 4768 5669 5931
Size: 197 926 301 542 290 1692 901 262
Cuts at: 5931 820
Size: 1553
Fragments arranged by size:
1692 1553 926 901 542 301 290 262
197
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 3867 4022 1132
Size: 2735 155 3774
Fragments arranged by size:
3774 2735 155
SspI AAT'ATT
Cuts at: 5 52 3549 4588 5
Size: 47 3497 1039 2081
Fragments arranged by size:
3497 2081 1039 47
StuI AGG'CCT
Cuts at: 1517 3884 1517
Size: 2367 4297
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 2632 3438 4103 513
Size: 552 939 588 40 806 665 3074
Fragments arranged by size:
3074 939 806 665 588 552 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2653 2694 2948 3905 4691 4708 4861
Size: 1255 1073 41 254 957 786 17 153
Cuts at: 4861 5046 5491 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1073 957 786 445 254 185
153 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2743 3064
Size: 274 80 33 51 1443 141 625 321
Cuts at: 3064 3476 4910 6622 96
Size: 412 1434 1712 138
Fragments arranged by size:
1712 1443 1434 625 412 321 274 141
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6624 6656 6624
Size: 32 6632
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6664
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2363 3227 3293 2185
Size: 178 864 66 5556
Fragments arranged by size:
5556 864 178 66
XhoI C'TCGA_G
Cuts at: 1943 2786 1943
Size: 843 5821
XmaI C'CCGG_G
Cuts at: 1870 2529 3989 1870
Size: 659 1460 4545
Fragments arranged by size:
4545 1460 659
XmnI GAAnn'nnTTC
Cuts at: 2745 3464 4793 2745
Size: 719 1329 4616
Fragments arranged by size:
4616 1329 719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI RsrII SacII SanDI
SfiI SfoI SrfI SwaI