Restriction map for
pFN21AB6168
(Circular) (Six-base) MAPSORT of: pFN21AB6168.seq Check: 9789 from: 1 to: 6514
pFN21AB6168 6514 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2963 3473 2963
Size: 510 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4324 278
Size: 53 83 186 936 2788 2468
Fragments arranged by size:
2788 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 3114 3857 1937
Size: 792 385 743 4594
Fragments arranged by size:
4594 792 743 385
Acc65I G'GTAC_C
Cuts at: 3835 3835
Size: 6514
AclI AA'CG_TT
Cuts at: 1563 4641 5014 1563
Size: 3078 373 3063
Fragments arranged by size:
3078 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2846 3497 4577 5961 6229 1632
Size: 1214 651 1080 1384 268 1917
Fragments arranged by size:
1917 1384 1214 1080 651 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6317
AflIII A'CryG_T
Cuts at: 1203 5413 6390 1203
Size: 4210 977 1327
Fragments arranged by size:
4210 1327 977
AgeI A'CCGG_T
Cuts at: 5345 5345
Size: 6514
AhdI GACnn_n'nnGTC
Cuts at: 5243 5243
Size: 6514
AleI CACnn'nnGTG
Cuts at: 1166 3126 3761 1166
Size: 1960 635 3919
Fragments arranged by size:
3919 1960 635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2270 1184
Size: 1086 5428
AlwNI CAG_nnn'CTG
Cuts at: 2021 2503 3145 4257 5829 2021
Size: 482 642 1112 1572 2706
Fragments arranged by size:
2706 1572 1112 642 482
ApaLI G'TGCA_C
Cuts at: 4571 5727 4571
Size: 1156 5358
ApoI r'AATT_y
Cuts at: 1493 3823 4049 4201 6310 1493
Size: 2330 226 152 2109 1697
Fragments arranged by size:
2330 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5068 160
Size: 872 4036 1606
Fragments arranged by size:
4036 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 3069 3839 1870
Size: 73 720 406 770 4545
Fragments arranged by size:
4545 770 720 406 73
BamHI G'GATC_C
Cuts at: 3208 3844 4211 3208
Size: 636 367 5511
Fragments arranged by size:
5511 636 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 3485 3835 5290 618
Size: 325 217 627 254 1444 350 1455 1842
Fragments arranged by size:
1842 1455 1444 627 350 325 254 217
BanII G_rGCy'C
Cuts at: 1391 2095 2400 3519 3782 3833 1391
Size: 704 305 1119 263 51 4072
Fragments arranged by size:
4072 1119 704 305 263 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2636 928
Size: 971 737 4806
Fragments arranged by size:
4806 971 737
BbvCI CC'TCA_GC
Cuts at: 3204 3578 3204
Size: 374 6140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4705 4739 1903
Size: 34 2768 34 3678
Fragments arranged by size:
3678 2768 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4406 5616 4406
Size: 1210 5304
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6514
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5125 136
Size: 107 122 71 631 605 3453 1525
Fragments arranged by size:
3453 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2211 2563 6509 2211
Size: 352 3946 2216
Fragments arranged by size:
3946 2216 352
BlpI GC'TnA_GC
Cuts at: 2672 3776 3931 2672
Size: 1104 155 5255
Fragments arranged by size:
5255 1104 155
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2688 3638 4575 5731 1790
Size: 254 644 950 937 1156 2573
Fragments arranged by size:
2573 1156 950 937 644 254
BmgBI CAC'GTC
Cuts at: 1318 2341 1318
Size: 1023 5491
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4245 5203 442
Size: 416 53 83 399 24 2828 958 1753
Fragments arranged by size:
2828 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 2588 3725 5174 1239
Size: 1202 147 1137 1449 2579
Fragments arranged by size:
2579 1449 1202 1137 147
Bpu10I CC'TnA_GC
Cuts at: 3060 3204 3578 3060
Size: 144 374 5996
Fragments arranged by size:
5996 374 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2519 3788 4639 5504 5802 2258
Size: 261 1269 851 865 298 2970
Fragments arranged by size:
2970 1269 865 851 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2418 2523 2771 5177 882
Size: 617 919 105 248 2406 2219
Fragments arranged by size:
2406 2219 919 617 248 105
BsaAI yAC'GTr
Cuts at: 493 1204 4305 493
Size: 711 3101 2702
Fragments arranged by size:
3101 2702 711
BsaBI GATnn'nnATC
Cuts at: 6508 6508
Size: 6514
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4321 4703 5338 275
Size: 53 83 186 936 2788 382 635 1451
Fragments arranged by size:
2788 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2787 3012 4947 5345 5619 5766 6177 2787
Size: 225 1935 398 274 147 411 3124
Fragments arranged by size:
3124 1935 411 398 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2282 2312 1178
Size: 30 165 30 879 30 5380
Fragments arranged by size:
5380 879 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2449 3212 3722 1186
Size: 1263 763 510 3978
Fragments arranged by size:
3978 1263 763 510
BseYI C'CCAG_C
Cuts at: 2539 2990 3005 5717 6213 2539
Size: 451 15 2712 496 2840
Fragments arranged by size:
2840 2712 496 451 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2411 2489 3131 3557 3614 1163
Size: 573 675 78 642 426 57 4063
Fragments arranged by size:
4063 675 642 573 426 78 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3964 4725 5753 1540
Size: 275 186 48 1915 761 1028 2301
Fragments arranged by size:
2301 1915 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2095 2400 3309 3471 3519 3782 4575 4660 5731
Size: 305 909 162 48 263 793 85 1071
Cuts at: 5731 2095
Size: 2878
Fragments arranged by size:
2878 1071 909 793 305 263 162 85
48
BsmI GAATG_Cn'
Cuts at: 4025 4118 4025
Size: 93 6421
BsmBI CGTCTCn'nnnn_
Cuts at: 2948 3806 2948
Size: 858 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2095 2400 2688 3309 3471 3519
Size: 399 254 51 305 288 621 162 48
Cuts at: 3519 3638 3782 3833 4575 4660 5731 1391
Size: 119 144 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 621 399 305 288 254
162 144 119 85 51 51 48
BspEI T'CCGG_A
Cuts at: 2787 6177 2787
Size: 3390 3124
BspHI T'CATG_A
Cuts at: 4403 6133 4403
Size: 1730 4784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2511 2963 3473 3869 844
Size: 1667 452 510 396 3489
Fragments arranged by size:
3489 1667 510 452 396
BsrBI CCG'CTC
Cuts at: 1394 1823 4401 1394
Size: 429 2578 3507
Fragments arranged by size:
3507 2578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5009 5183 65
Size: 1183 3761 174 1396
Fragments arranged by size:
3761 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2110 2151 3738 5158 5345 1700
Size: 110 234 66 41 1587 1420 187 2869
Fragments arranged by size:
2869 1587 1420 234 187 110 66 41
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6514
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6514
BssSI C'ACGA_G
Cuts at: 4574 5586 4574
Size: 1012 5502
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2021 2485 3145 794
Size: 1227 464 660 4163
Fragments arranged by size:
4163 1227 660 464
BstBI TT'CG_AA
Cuts at: 5318 5318
Size: 6514
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6514
BstXI CCAn_nnnn'nTGG
Cuts at: 2998 2998
Size: 6514
BstYI r'GATC_y
Cuts at: 1972 2121 2211 2563 2869 3208 3844 4211 4596
Size: 149 90 352 306 339 636 367 385
Cuts at: 4596 4613 6065 6151 6163 6509 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 636 385 367 352 346 339
306 149 90 86 17 12
BstZ17I GTA'TAC
Cuts at: 2730 2730
Size: 6514
Bsu36I CC'TnA_GG
Cuts at: 2457 2457
Size: 6514
BtgI C'CryG_G
Cuts at: 513 1065 2301 2353 2832 513
Size: 552 1236 52 479 4195
Fragments arranged by size:
4195 1236 552 479 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5775
Fragments arranged by size:
5775 683 56
BtsI GCAGTG_nn'
Cuts at: 2022 2874 4030 4824 4844 2022
Size: 852 1156 794 20 3692
Fragments arranged by size:
3692 1156 852 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6514
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6479
DraI TTT'AAA
Cuts at: 3818 3818
Size: 6514
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5521 809
Size: 507 4205 1802
Fragments arranged by size:
4205 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2835 3732
Size: 54 1425 50 275 18 216 789 897
Cuts at: 3732 3789 3961 4850 6479 8
Size: 57 172 889 1629 43
Fragments arranged by size:
1629 1425 897 889 789 275 216 172
57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3961 1537
Size: 275 234 1915 4090
Fragments arranged by size:
4090 1915 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 3314 4444 1353
Size: 1961 1130 3423
Fragments arranged by size:
3423 1961 1130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5101 5475 5621 387
Size: 1064 138 3512 374 146 1280
Fragments arranged by size:
3512 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2093 2398 3517 3780 2093
Size: 305 1119 263 4827
Fragments arranged by size:
4827 1119 305 263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2588 2846 3497 3725 4577 5174
Size: 393 809 147 258 651 228 852 597
Cuts at: 5174 5961 6229 1239
Size: 787 268 1524
Fragments arranged by size:
1524 852 809 787 651 597 393 268
258 228 147
EcoO109I rG'GnC_Cy
Cuts at: 2068 2131 2068
Size: 63 6451
EcoRI G'AATT_C
Cuts at: 3823 4201 3823
Size: 378 6136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 3646 3694 2209
Size: 1437 48 5029
Fragments arranged by size:
5029 1437 48
FspI TGC'GCA
Cuts at: 5020 5020
Size: 6514
HaeII r_GCGC'y
Cuts at: 1678 2161 3564 5661 1678
Size: 483 1403 2097 2531
Fragments arranged by size:
2531 2097 1403 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 2693 2725 3391
Size: 32 135 32 710 32 379 32 666
Cuts at: 3391 3423 5155 5187 5229 5261 1373
Size: 32 1732 32 42 32 2626
Fragments arranged by size:
2626 1732 710 666 379 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3115 3858 4104 669
Size: 988 281 1177 743 246 3079
Fragments arranged by size:
3079 1177 988 743 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6514
HpaI GTT'AAC
Cuts at: 4104 4104
Size: 6514
KpnI G_GTAC'C
Cuts at: 3839 3839
Size: 6514
MfeI C'AATT_G
Cuts at: 4113 4113
Size: 6514
MluI A'CGCG_T
Cuts at: 6390 6390
Size: 6514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2373 5628 5812 1305
Size: 1068 3255 184 2007
Fragments arranged by size:
3255 2007 1068 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3734 10
Size: 54 1425 343 1902 2790
Fragments arranged by size:
2790 1902 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3126 3761 4472 4831 4990 518
Size: 648 1960 635 711 359 159 2042
Fragments arranged by size:
2042 1960 711 648 635 359 159
MspA1I CmG'CkG
Cuts at: 1606 3145 3930 4607 5755 6000 1606
Size: 1539 785 677 1148 245 2120
Fragments arranged by size:
2120 1539 1148 785 677 245
NaeI GCC'GGC
Cuts at: 2153 3740 2153
Size: 1587 4927
NcoI C'CATG_G
Cuts at: 513 1065 2301 513
Size: 552 1236 4726
Fragments arranged by size:
4726 1236 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6514
NgoMIV G'CCGG_C
Cuts at: 2151 3738 2151
Size: 1587 4927
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6514
NotI GC'GGCC_GC
Cuts at: 3961 3961
Size: 6514
NspI r_CATG'y
Cuts at: 1132 1229 3136 3872 5417 1132
Size: 97 1907 736 1545 2229
Fragments arranged by size:
2229 1907 1545 736 97
PasI CC'CwG_GG
Cuts at: 1348 2054 1348
Size: 706 5808
PciI A'CATG_T
Cuts at: 5413 5413
Size: 6514
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3231 1108
Size: 2123 4391
PfoI T'CCnGG_A
Cuts at: 2086 2124 2418 3609 3702 2086
Size: 38 294 1191 93 4898
Fragments arranged by size:
4898 1191 294 93 38
PmeI GTTT'AAAC
Cuts at: 3818 3818
Size: 6514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4583 4615 6096 6128 4583
Size: 32 1481 32 4969
Fragments arranged by size:
4969 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2131 2068
Size: 63 6451
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6514
PsiI TTA'TAA
Cuts at: 4084 4084
Size: 6514
PstI C_TGCA'G
Cuts at: 830 2017 2026 3141 3360 3866 830
Size: 1187 9 1115 219 506 3478
Fragments arranged by size:
3478 1187 1115 506 219 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6514
PvuII CAG'CTG
Cuts at: 3145 3145
Size: 6514
SacI G_AGCT'C
Cuts at: 2095 2400 3519 3782 2095
Size: 305 1119 263 4827
Fragments arranged by size:
4827 1119 305 263
SalI G'TCGA_C
Cuts at: 1936 3113 3856 1936
Size: 1177 743 4594
Fragments arranged by size:
4594 1177 743
SanDI GG'GwC_CC
Cuts at: 2068 2068
Size: 6514
SapI GCTCTTCn'nnn_
Cuts at: 3314 3314
Size: 6514
SbfI CC_TGCA'GG
Cuts at: 3866 3866
Size: 6514
ScaI AGT'ACT
Cuts at: 4762 6474 4762
Size: 1712 4802
SexAI A'CCwGG_T
Cuts at: 3542 3542
Size: 6514
SfcI C'TryA_G
Cuts at: 826 1045 2013 2022 3137 3356 3862 4997 5678
Size: 219 968 9 1115 219 506 1135 681
Cuts at: 5678 5869 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1115 968 681 506 219 219
191 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
SgrAI Cr'CCGG_yG
Cuts at: 1700 2110 1700
Size: 410 6104
SmaI CCC'GGG
Cuts at: 1872 3071 3841 1872
Size: 1199 770 4545
Fragments arranged by size:
4545 1199 770
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 2534 3803 4618 5519 5781
Size: 197 926 330 261 1269 815 901 262
Cuts at: 5781 820
Size: 1553
Fragments arranged by size:
1553 1269 926 901 815 330 262 261
197
SnaBI TAC'GTA
Cuts at: 493 4305 493
Size: 3812 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6514
SphI G_CATG'C
Cuts at: 1132 3872 1132
Size: 2740 3774
SspI AAT'ATT
Cuts at: 5 52 3446 4438 5
Size: 47 3394 992 2081
Fragments arranged by size:
3394 2081 992 47
StuI AGG'CCT
Cuts at: 1517 3809 1517
Size: 2292 4222
StyI C'CwwG_G
Cuts at: 513 1065 2229 2301 2972 3271 3953 513
Size: 552 1164 72 671 299 682 3074
Fragments arranged by size:
3074 1164 682 671 552 299 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2706 3235 4541 4558 4711 4896 5341
Size: 1255 1126 529 1306 17 153 185 445
Cuts at: 5341 325
Size: 1498
Fragments arranged by size:
1498 1306 1255 1126 529 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3360 4760 6472
Size: 274 80 33 51 1443 1383 1400 1712
Cuts at: 6472 96
Size: 138
Fragments arranged by size:
1712 1443 1400 1383 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3315 3347 6474 6506 3315
Size: 32 3127 32 3323
Fragments arranged by size:
3323 3127 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6514
XbaI T'CTAG_A
Cuts at: 3850 3850
Size: 6514
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6514
XmaI C'CCGG_G
Cuts at: 1870 3069 3839 1870
Size: 1199 770 4545
Fragments arranged by size:
4545 1199 770
XmnI GAAnn'nnTTC
Cuts at: 4643 4643
Size: 6514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4322 276
Size: 53 83 186 936 2788 2468
Fragments arranged by size:
2788 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI DraIII EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SfiI SfoI SrfI SwaI XcmI