Restriction map for
pFN21AB6166
(Circular) (Six-base) MAPSORT of: pFN21AB6166.seq Check: 6775 from: 1 to: 6514
pFN21AB6166 6514 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2225 2292 2225
Size: 67 6447
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2374 4324 278
Size: 53 83 186 936 838 1950 2468
Fragments arranged by size:
2468 1950 936 838 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3857 1937
Size: 1920 4594
Acc65I G'GTAC_C
Cuts at: 3835 3835
Size: 6514
AclI AA'CG_TT
Cuts at: 1563 4641 5014 1563
Size: 3078 373 3063
Fragments arranged by size:
3078 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3511 3530 3720 4577 5961 6229 1632
Size: 1879 19 190 857 1384 268 1917
Fragments arranged by size:
1917 1879 1384 857 268 190 19
AflII C'TTAA_G
Cuts at: 820 1017 2905 820
Size: 197 1888 4429
Fragments arranged by size:
4429 1888 197
AflIII A'CryG_T
Cuts at: 1203 3121 5413 6390 1203
Size: 1918 2292 977 1327
Fragments arranged by size:
2292 1918 1327 977
AgeI A'CCGG_T
Cuts at: 5345 5345
Size: 6514
AhdI GACnn_n'nnGTC
Cuts at: 5243 5243
Size: 6514
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6514
AlwNI CAG_nnn'CTG
Cuts at: 4257 5829 4257
Size: 1572 4942
ApaLI G'TGCA_C
Cuts at: 3173 4571 5727 3173
Size: 1398 1156 3960
Fragments arranged by size:
3960 1398 1156
ApoI r'AATT_y
Cuts at: 1493 2434 2933 3572 3596 3823 4049 4201 6310
Size: 941 499 639 24 227 226 152 2109
Cuts at: 6310 1493
Size: 1697
Fragments arranged by size:
2109 1697 941 639 499 227 226 152
24
AseI AT'TA_AT
Cuts at: 160 1032 2475 5068 160
Size: 872 1443 2593 1606
Fragments arranged by size:
2593 1606 1443 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
AvaI C'yCGr_G
Cuts at: 1870 1943 3839 1870
Size: 73 1896 4545
Fragments arranged by size:
4545 1896 73
BamHI G'GATC_C
Cuts at: 3844 4211 3844
Size: 367 6147
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3835 5290 618
Size: 325 217 627 2048 1455 1842
Fragments arranged by size:
2048 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3029 3833 1391
Size: 1638 804 4072
Fragments arranged by size:
4072 1638 804
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3368 3380 928
Size: 971 1469 12 4062
Fragments arranged by size:
4062 1469 971 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4705 4739 1903
Size: 34 2768 34 3678
Fragments arranged by size:
3678 2768 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3485 4406 5616 3485
Size: 921 1210 4383
Fragments arranged by size:
4383 1210 921
BclI T'GATC_A
Cuts at: 1548 2110 1548
Size: 562 5952
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5125 136
Size: 107 122 71 631 605 3453 1525
Fragments arranged by size:
3453 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2863 6509 2863
Size: 3646 2868
BlpI GC'TnA_GC
Cuts at: 3931 3931
Size: 6514
Bme1580I G_kGCm'C
Cuts at: 1790 3177 3229 4575 5731 1790
Size: 1387 52 1346 1156 2573
Fragments arranged by size:
2573 1387 1346 1156 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6514
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2088 3232 3316
Size: 416 53 83 399 24 671 1144 84
Cuts at: 3316 4245 5203 442
Size: 929 958 1753
Fragments arranged by size:
1753 1144 958 929 671 416 399 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 6514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2208 2292 2766 3024 3087 3181 5174 1239
Size: 969 84 474 258 63 94 1993 2579
Fragments arranged by size:
2579 1993 969 474 258 94 84 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4639 5504 5802 4639
Size: 865 298 5351
Fragments arranged by size:
5351 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3725 5177 882
Size: 617 2226 1452 2219
Fragments arranged by size:
2226 2219 1452 617
BsaAI yAC'GTr
Cuts at: 493 1204 4305 493
Size: 711 3101 2702
Fragments arranged by size:
3101 2702 711
BsaBI GATnn'nnATC
Cuts at: 6508 6508
Size: 6514
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2371 4321 4703 5338
Size: 53 83 186 936 838 1950 382 635
Cuts at: 5338 275
Size: 1451
Fragments arranged by size:
1950 1451 936 838 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4947 5345 5619 5766 6177 4947
Size: 398 274 147 411 5284
Fragments arranged by size:
5284 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2046 2076 2647 2677 1178
Size: 30 165 30 643 30 571 30 5015
Fragments arranged by size:
5015 643 571 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6514
BseYI C'CCAG_C
Cuts at: 5717 6213 5717
Size: 496 6018
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2259 1163
Size: 573 523 5418
Fragments arranged by size:
5418 573 523
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3964 4725 5753 1540
Size: 275 186 1963 761 1028 2301
Fragments arranged by size:
2301 1963 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3014 3029 3177 3438 4575 4660 5731 3014
Size: 15 148 261 1137 85 1071 3797
Fragments arranged by size:
3797 1137 1071 261 148 85 15
BsmI GAATG_Cn'
Cuts at: 3691 4025 4118 3691
Size: 334 93 6087
Fragments arranged by size:
6087 334 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3014 3029 3177 3229 3438 3833 4575
Size: 399 1224 15 148 52 209 395 742
Cuts at: 4575 4660 5731 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1224 1071 742 399 395 209 148
85 52 15
BspEI T'CCGG_A
Cuts at: 6177 6177
Size: 6514
BspHI T'CATG_A
Cuts at: 2727 4403 6133 2727
Size: 1676 1730 3108
Fragments arranged by size:
3108 1730 1676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2225 2292 2499 3869 844
Size: 1381 67 207 1370 3489
Fragments arranged by size:
3489 1381 1370 207 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4401 1394
Size: 429 2578 3507
Fragments arranged by size:
3507 2578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2917 5009 5183 65
Size: 1183 1669 2092 174 1396
Fragments arranged by size:
2092 1669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5158 5345 1700
Size: 110 3348 187 2869
Fragments arranged by size:
3348 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3052 96
Size: 2956 3558
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6514
BssSI C'ACGA_G
Cuts at: 2583 4574 5586 2583
Size: 1991 1012 3511
Fragments arranged by size:
3511 1991 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3018 794
Size: 2224 4290
BstBI TT'CG_AA
Cuts at: 5318 5318
Size: 6514
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6514
BstYI r'GATC_y
Cuts at: 1972 2863 3844 4211 4596 4613 6065 6151 6163
Size: 891 981 367 385 17 1452 86 12
Cuts at: 6163 6509 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 981 891 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5023
Fragments arranged by size:
5023 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5775
Fragments arranged by size:
5775 683 56
BtsI GCAGTG_nn'
Cuts at: 4030 4824 4844 4030
Size: 794 20 5700
Fragments arranged by size:
5700 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6514
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3744 3779 529
Size: 35 3180 35 3264
Fragments arranged by size:
3264 3180 35 35
DraI TTT'AAA
Cuts at: 2416 3818 2416
Size: 1402 5112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5521 809
Size: 507 4205 1802
Fragments arranged by size:
4205 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2820 3961
Size: 54 1425 50 275 18 351 639 1141
Cuts at: 3961 4850 6479 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1141 889 639 351 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3961 1537
Size: 275 2149 4090
Fragments arranged by size:
4090 2149 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3530 4444 1353
Size: 2177 914 3423
Fragments arranged by size:
3423 2177 914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5101 5475 5621 387
Size: 1064 138 3512 374 146 1280
Fragments arranged by size:
3512 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3027 3027
Size: 6514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2292 2766 3024 3087 3181 3511
Size: 393 576 84 474 258 63 94 330
Cuts at: 3511 3530 3720 4577 5174 5961 6229 1239
Size: 19 190 857 597 787 268 1524
Fragments arranged by size:
1524 857 787 597 576 474 393 330
268 258 190 94 84 63 19
EcoO109I rG'GnC_Cy
Cuts at: 2638 2638
Size: 6514
EcoRI G'AATT_C
Cuts at: 3596 3823 4201 3596
Size: 227 378 5909
Fragments arranged by size:
5909 378 227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2231 2231
Size: 6514
FspI TGC'GCA
Cuts at: 5020 5020
Size: 6514
HaeII r_GCGC'y
Cuts at: 1678 5661 1678
Size: 3983 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 2906 2938 3389
Size: 32 135 32 776 32 526 32 451
Cuts at: 3389 3421 3642 3674 3773 3805 5155 5187 5229
Size: 32 221 32 99 32 1350 32 42
Cuts at: 5229 5261 1373
Size: 32 2626
Fragments arranged by size:
2626 1350 776 526 451 221 135 99
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 2700 3408 3858 4104 669
Size: 988 281 304 458 708 450 246 3079
Fragments arranged by size:
3079 988 708 458 450 304 281 246
HindIII A'AGCT_T
Cuts at: 748 2902 3285 748
Size: 2154 383 3977
Fragments arranged by size:
3977 2154 383
HpaI GTT'AAC
Cuts at: 4104 4104
Size: 6514
KpnI G_GTAC'C
Cuts at: 3839 3839
Size: 6514
MfeI C'AATT_G
Cuts at: 3370 4113 3370
Size: 743 5771
MluI A'CGCG_T
Cuts at: 6390 6390
Size: 6514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2622 2690 5628 5812 1305
Size: 1317 68 2938 184 2007
Fragments arranged by size:
2938 2007 1317 184 68
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2183 2822 10
Size: 54 1425 343 351 639 3702
Fragments arranged by size:
3702 1425 639 351 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4472 4831 4990 518
Size: 648 3306 359 159 2042
Fragments arranged by size:
3306 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2496 2765 3930 4607 5755 6000 1606
Size: 890 269 1165 677 1148 245 2120
Fragments arranged by size:
2120 1165 1148 890 677 269 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5023
Fragments arranged by size:
5023 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6514
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6514
NotI GC'GGCC_GC
Cuts at: 3961 3961
Size: 6514
NspI r_CATG'y
Cuts at: 1132 1229 3125 3872 5417 1132
Size: 97 1896 747 1545 2229
Fragments arranged by size:
2229 1896 1545 747 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6514
PciI A'CATG_T
Cuts at: 3121 5413 3121
Size: 2292 4222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6514
PfoI T'CCnGG_A
Cuts at: 2200 3001 2200
Size: 801 5713
PmeI GTTT'AAAC
Cuts at: 3818 3818
Size: 6514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4583 4615 6096 6128 4583
Size: 32 1481 32 4969
Fragments arranged by size:
4969 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2638 2638
Size: 6514
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6514
PsiI TTA'TAA
Cuts at: 3786 4084 3786
Size: 298 6216
PstI C_TGCA'G
Cuts at: 830 2289 2510 3781 3866 830
Size: 1459 221 1271 85 3478
Fragments arranged by size:
3478 1459 1271 221 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6514
PvuII CAG'CTG
Cuts at: 2765 2765
Size: 6514
SacI G_AGCT'C
Cuts at: 3029 3029
Size: 6514
SalI G'TCGA_C
Cuts at: 1936 3856 1936
Size: 1920 4594
SbfI CC_TGCA'GG
Cuts at: 3866 3866
Size: 6514
ScaI AGT'ACT
Cuts at: 3522 4762 6474 3522
Size: 1240 1712 3562
Fragments arranged by size:
3562 1712 1240
SfcI C'TryA_G
Cuts at: 826 1045 2285 2506 3449 3637 3777 3862 4997
Size: 219 1240 221 943 188 140 85 1135
Cuts at: 4997 5678 5869 826
Size: 681 191 1471
Fragments arranged by size:
1471 1240 1135 943 681 221 219 191
188 140 85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6514
SmaI CCC'GGG
Cuts at: 1872 3841 1872
Size: 1969 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2905 4618 5519 5781 820
Size: 197 926 962 1713 901 262 1553
Fragments arranged by size:
1713 1553 962 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4305 493
Size: 3812 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6514
SphI G_CATG'C
Cuts at: 1132 3872 1132
Size: 2740 3774
SspI AAT'ATT
Cuts at: 5 52 2734 4438 5
Size: 47 2682 1704 2081
Fragments arranged by size:
2682 2081 1704 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6514
StyI C'CwwG_G
Cuts at: 513 1065 2004 2122 2314 3953 513
Size: 552 939 118 192 1639 3074
Fragments arranged by size:
3074 1639 939 552 192 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4541 4558 4711 4896 5341 325
Size: 1255 2961 17 153 185 445 1498
Fragments arranged by size:
2961 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2690 2813 3052
Size: 274 80 33 51 1443 713 123 239
Cuts at: 3052 3520 4760 6472 96
Size: 468 1240 1712 138
Fragments arranged by size:
1712 1443 1240 713 468 274 239 138
123 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6474 6506 6474
Size: 32 6482
Tth111I GACn'n_nGTC
Cuts at: 1316 2549 1316
Size: 1233 5281
XbaI T'CTAG_A
Cuts at: 3850 3850
Size: 6514
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6514
XmaI C'CCGG_G
Cuts at: 1870 3839 1870
Size: 1969 4545
XmnI GAAnn'nnTTC
Cuts at: 4643 4643
Size: 6514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2372 4322 276
Size: 53 83 186 936 838 1950 2468
Fragments arranged by size:
2468 1950 936 838 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI