Restriction map for
pFN21AB6163
(Circular) (Six-base) MAPSORT of: pFN21AB6163.seq Check: 1066 from: 1 to: 6508
pFN21AB6163 6508 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 3851 1937
Size: 1593 321 4594
Fragments arranged by size:
4594 1593 321
Acc65I G'GTAC_C
Cuts at: 3463 3829 3463
Size: 366 6142
AclI AA'CG_TT
Cuts at: 1563 4635 5008 1563
Size: 3072 373 3063
Fragments arranged by size:
3072 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2373 4571 5955 6223 1632
Size: 741 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 741 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 5407 6384 1203
Size: 4204 977 1327
Fragments arranged by size:
4204 1327 977
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 5237 5237
Size: 6508
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3248 3258 1184
Size: 2064 10 4434
Fragments arranged by size:
4434 2064 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 6476
AlwNI CAG_nnn'CTG
Cuts at: 2282 2396 2965 3691 3745 4251 5823 2282
Size: 114 569 726 54 506 1572 2967
Fragments arranged by size:
2967 1572 726 569 506 114 54
ApaI G_GGCC'C
Cuts at: 2433 2809 2433
Size: 376 6132
ApaLI G'TGCA_C
Cuts at: 3077 4565 5721 3077
Size: 1488 1156 3864
Fragments arranged by size:
3864 1488 1156
ApoI r'AATT_y
Cuts at: 1493 3817 4043 4195 6304 1493
Size: 2324 226 152 2109 1697
Fragments arranged by size:
2324 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5062 160
Size: 872 4030 1606
Fragments arranged by size:
4030 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 2895 3032 3393 3536 3833 1870
Size: 73 952 137 361 143 297 4545
Fragments arranged by size:
4545 952 361 297 143 137 73
AvrII C'CTAG_G
Cuts at: 2515 2515
Size: 6508
BamHI G'GATC_C
Cuts at: 2453 3838 4205 2453
Size: 1385 367 4756
Fragments arranged by size:
4756 1385 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3050 3463 3829 5284 618
Size: 325 217 627 1263 413 366 1455 1842
Fragments arranged by size:
1842 1455 1263 627 413 366 325 217
BanII G_rGCy'C
Cuts at: 1391 2232 2433 2528 2809 2880 2988 3008 3797
Size: 841 201 95 281 71 108 20 789
Cuts at: 3797 3827 1391
Size: 30 4072
Fragments arranged by size:
4072 841 789 281 201 108 95 71
30 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3334 928
Size: 971 1435 4102
Fragments arranged by size:
4102 1435 971
BbvCI CC'TCA_GC
Cuts at: 2320 3508 2320
Size: 1188 5320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3245 3279 4699 4733 1903
Size: 34 1308 34 1420 34 3678
Fragments arranged by size:
3678 1420 1308 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3404 5119 136
Size: 107 122 71 631 605 1732 1715 1525
Fragments arranged by size:
1732 1715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3171 6503 3171
Size: 3332 3176
BlpI GC'TnA_GC
Cuts at: 2145 3925 2145
Size: 1780 4728
Bme1580I G_kGCm'C
Cuts at: 1790 2433 2809 3053 3081 4569 5725 1790
Size: 643 376 244 28 1488 1156 2573
Fragments arranged by size:
2573 1488 1156 643 376 244 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2454 3332 4239
Size: 416 53 83 399 24 1037 878 907
Cuts at: 4239 5197 442
Size: 958 1753
Fragments arranged by size:
1753 1037 958 907 878 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3120 1056
Size: 2064 4444
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 3005 3391 2143
Size: 862 386 5260
Fragments arranged by size:
5260 862 386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2853 3022 3149 3713 5168 1239
Size: 1614 169 127 564 1455 2579
Fragments arranged by size:
2579 1614 1455 564 169 127
Bpu10I CC'TnA_GC
Cuts at: 2320 2810 3508 2320
Size: 490 698 5320
Fragments arranged by size:
5320 698 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2253 2840 4633 5498 5796 2171
Size: 82 587 1793 865 298 2883
Fragments arranged by size:
2883 1793 865 587 298 82
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5171 882
Size: 617 3672 2219
Fragments arranged by size:
3672 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4299 493
Size: 711 3095 2702
Fragments arranged by size:
3095 2702 711
BsaBI GATnn'nnATC
Cuts at: 6502 6502
Size: 6508
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3665 4315 4697 5332
Size: 53 83 186 936 2132 650 382 635
Cuts at: 5332 275
Size: 1451
Fragments arranged by size:
2132 1451 936 650 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3038 3053 4941 5339 5613 5760 6171 3038
Size: 15 1888 398 274 147 411 3375
Fragments arranged by size:
3375 1888 411 398 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2404 2407 2434 2437 2867
Size: 30 165 30 1001 3 27 3 430
Cuts at: 2867 2897 3562 3592 1178
Size: 30 665 30 4094
Fragments arranged by size:
4094 1001 665 430 165 30 30 30
30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2428 3152 1186
Size: 1242 724 4542
Fragments arranged by size:
4542 1242 724
BseYI C'CCAG_C
Cuts at: 2361 2444 2506 2958 3186 3467 3499 3600 3790
Size: 83 62 452 228 281 32 101 190
Cuts at: 3790 3798 5711 6207 2361
Size: 8 1913 496 2662
Fragments arranged by size:
2662 1913 496 452 281 228 190 101
83 62 32 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3680 1163
Size: 573 1944 3991
Fragments arranged by size:
3991 1944 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2095 3958 4719 5747 1540
Size: 275 186 94 1863 761 1028 2301
Fragments arranged by size:
2301 1863 1028 761 275 186 94
BsiHKAI G_wGCw'C
Cuts at: 3081 4569 4654 5725 3081
Size: 1488 85 1071 3864
Fragments arranged by size:
3864 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 4019 4112 4019
Size: 93 6415
BsmBI CGTCTCn'nnnn_
Cuts at: 2021 2021
Size: 6508
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2433 2528 2809 2880 2988 3008
Size: 399 442 201 95 281 71 108 20
Cuts at: 3008 3053 3081 3797 3827 4569 4654 5725 1391
Size: 45 28 716 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 716 442 399 281 201
108 95 85 71 45 30 28 20
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 2736 4397 6127 2736
Size: 1661 1730 3117
Fragments arranged by size:
3117 1730 1661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3744 3863 844
Size: 2900 119 3489
Fragments arranged by size:
3489 2900 119
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 2695 3289 4395 1394
Size: 429 196 676 594 1106 3507
Fragments arranged by size:
3507 1106 676 594 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 3407 5003 5177 65
Size: 1183 2159 1596 174 1396
Fragments arranged by size:
2159 1596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5152 5339 1700
Size: 110 3342 187 2869
Fragments arranged by size:
3342 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2859 96
Size: 2763 3745
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 4568 5580 4568
Size: 1012 5496
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2557 3355 3409 3613 794
Size: 1763 798 54 204 3689
Fragments arranged by size:
3689 1763 798 204 54
BstBI TT'CG_AA
Cuts at: 5312 5312
Size: 6508
BstEII G'GTnAC_C
Cuts at: 1184 2047 1184
Size: 863 5645
BstXI CCAn_nnnn'nTGG
Cuts at: 2539 2539
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 2181 2272 2453 3171 3417 3838 4205 4590
Size: 209 91 181 718 246 421 367 385
Cuts at: 4590 4607 6059 6145 6157 6503 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 718 421 385 367 346 246
209 181 91 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3708 513
Size: 552 939 1704 3313
Fragments arranged by size:
3313 1704 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5769
Fragments arranged by size:
5769 683 56
BtsI GCAGTG_nn'
Cuts at: 2286 2386 2407 2425 4024 4818 4838 2286
Size: 100 21 18 1599 794 20 3956
Fragments arranged by size:
3956 1599 794 100 21 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6473
DraI TTT'AAA
Cuts at: 3812 3812
Size: 6508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5515 809
Size: 507 4199 1802
Fragments arranged by size:
4199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2664 2931 3955
Size: 54 1425 50 275 18 834 267 1024
Cuts at: 3955 4844 6473 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1024 889 834 275 267 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3955 1537
Size: 275 2143 4090
Fragments arranged by size:
4090 2143 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2392 2929 3296 4438 1353
Size: 1039 537 367 1142 3423
Fragments arranged by size:
3423 1142 1039 537 367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2840 3314 5095 5469 5615
Size: 1064 138 435 816 474 1781 374 146
Cuts at: 5615 387
Size: 1280
Fragments arranged by size:
1781 1280 1064 816 474 435 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2373 2853 3022 3149 3713 4571 5168
Size: 393 741 480 169 127 564 858 597
Cuts at: 5168 5955 6223 1239
Size: 787 268 1524
Fragments arranged by size:
1524 858 787 741 597 564 480 393
268 169 127
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 2618
Size: 6508
EcoO109I rG'GnC_Cy
Cuts at: 2034 2429 2430 2806 3028 3451 3541 3742 2034
Size: 395 1 376 222 423 90 201 4800
Fragments arranged by size:
4800 423 395 376 222 201 90 1
EcoRI G'AATT_C
Cuts at: 3817 4195 3817
Size: 378 6130
EcoRV GAT'ATC
Cuts at: 3489 3489
Size: 6508
FspI TGC'GCA
Cuts at: 3224 5014 3224
Size: 1790 4718
HaeII r_GCGC'y
Cuts at: 1678 3600 5655 1678
Size: 1922 2055 2531
Fragments arranged by size:
2531 2055 1922
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2084 2088 2109 2116
Size: 32 135 32 505 7 4 21 7
Cuts at: 2116 2120 2303 2335 2563 2576 2595 2608 2615
Size: 4 183 32 228 13 19 13 7
Cuts at: 2615 2647 2936 2962 2968 2994 3750 3782 5149
Size: 32 289 26 6 26 756 32 1367
Cuts at: 5149 5181 5223 5255 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1367 756 505 289 228 183 135
42 32 32 32 32 32 32 32
26 26 21 19 13 13 7 7
7 6 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3852 4098 669
Size: 988 281 1914 246 3079
Fragments arranged by size:
3079 1914 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6508
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KpnI G_GTAC'C
Cuts at: 3467 3833 3467
Size: 366 6142
MfeI C'AATT_G
Cuts at: 4107 4107
Size: 6508
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2967 3605 5622 5806 1305
Size: 1662 638 2017 184 2007
Fragments arranged by size:
2017 2007 1662 638 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2666 10
Size: 54 1425 343 834 3852
Fragments arranged by size:
3852 1425 834 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 4466 4825 4984 518
Size: 648 1494 1806 359 159 2042
Fragments arranged by size:
2042 1806 1494 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2279 2563 2569 3190 3691 3924 4601
Size: 552 121 284 6 621 501 233 677
Cuts at: 4601 5749 5994 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 621 552 501 284 245
233 121 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 3708 513
Size: 552 939 1704 3313
Fragments arranged by size:
3313 1704 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 3116 1052
Size: 2064 4444
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 2719 2860 3223 3866 5411 1132
Size: 97 1490 141 363 643 1545 2229
Fragments arranged by size:
2229 1545 1490 643 363 141 97
PasI CC'CwG_GG
Cuts at: 1348 3455 1348
Size: 2107 4401
PciI A'CATG_T
Cuts at: 5407 5407
Size: 6508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3634 1108
Size: 2526 3982
PfoI T'CCnGG_A
Cuts at: 2129 2619 2871 3491 2129
Size: 490 252 620 5146
Fragments arranged by size:
5146 620 490 252
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 4577 4609 6090 6122 2867
Size: 32 1678 32 1481 32 3253
Fragments arranged by size:
3253 1678 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6508
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PspOMI G'GGCC_C
Cuts at: 2429 2805 2429
Size: 376 6132
PstI C_TGCA'G
Cuts at: 830 2550 3315 3333 3351 3417 3741 3860 830
Size: 1720 765 18 18 66 324 119 3478
Fragments arranged by size:
3478 1720 765 324 119 66 18 18
PvuI CG_AT'CG
Cuts at: 2001 2095 2001
Size: 94 6414
PvuII CAG'CTG
Cuts at: 2158 2279 2569 3190 3691 2158
Size: 121 290 621 501 4975
Fragments arranged by size:
4975 621 501 290 121
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SapI GCTCTTCn'nnn_
Cuts at: 3296 3296
Size: 6508
SbfI CC_TGCA'GG
Cuts at: 3741 3860 3741
Size: 119 6389
ScaI AGT'ACT
Cuts at: 4756 6468 4756
Size: 1712 4796
SfcI C'TryA_G
Cuts at: 826 1045 2546 3311 3329 3347 3413 3431 3737
Size: 219 1501 765 18 18 66 18 306
Cuts at: 3737 3856 4991 5672 5863 826
Size: 119 1135 681 191 1471
Fragments arranged by size:
1501 1471 1135 765 681 306 219 191
119 66 18 18 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 2897 3034 3835 1872
Size: 1025 137 801 4545
Fragments arranged by size:
4545 1025 801 137
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 2268 2819 4612 5513 5775
Size: 197 926 243 82 551 1793 901 262
Cuts at: 5775 820
Size: 1553
Fragments arranged by size:
1793 1553 926 901 551 262 243 197
82
SnaBI TAC'GTA
Cuts at: 493 4299 493
Size: 3806 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3866 1132
Size: 2734 3774
SspI AAT'ATT
Cuts at: 5 52 4432 5
Size: 47 4380 2081
Fragments arranged by size:
4380 2081 47
StuI AGG'CCT
Cuts at: 1517 2520 1517
Size: 1003 5505
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 2515 3472 3708 3947 513
Size: 552 939 60 451 957 236 239 3074
Fragments arranged by size:
3074 957 939 552 451 239 236 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 3461 4535 4552 4705 4890 5335
Size: 1255 512 1369 1074 17 153 185 445
Cuts at: 5335 325
Size: 1498
Fragments arranged by size:
1498 1369 1255 1074 512 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 4754 6466
Size: 274 80 33 51 1443 882 1895 1712
Cuts at: 6466 96
Size: 138
Fragments arranged by size:
1895 1712 1443 882 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2404 2407 2436 2439 3363 3395 3562 3594 6468
Size: 3 29 3 924 32 167 32 2874
Cuts at: 6468 6500 2404
Size: 32 2412
Fragments arranged by size:
2874 2412 924 167 32 32 32 29
3 3
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6508
XbaI T'CTAG_A
Cuts at: 3844 3844
Size: 6508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3635 3635
Size: 6508
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6508
XmaI C'CCGG_G
Cuts at: 1870 2895 3032 3833 1870
Size: 1025 137 801 4545
Fragments arranged by size:
4545 1025 801 137
XmnI GAAnn'nnTTC
Cuts at: 4637 4637
Size: 6508
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI