Restriction map for
pFN21AB6162
(Circular) (Six-base) MAPSORT of: pFN21AB6162.seq Check: 8579 from: 1 to: 6646
pFN21AB6162 6646 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 3989 1937
Size: 1161 891 4594
Fragments arranged by size:
4594 1161 891
Acc65I G'GTAC_C
Cuts at: 3326 3902 3967 3326
Size: 576 65 6005
Fragments arranged by size:
6005 576 65
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3484 4709 6093 6361 1632
Size: 1852 1225 1384 268 1917
Fragments arranged by size:
1917 1852 1384 1225 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6449
AflIII A'CryG_T
Cuts at: 1203 2271 5545 6522 1203
Size: 1068 3274 977 1327
Fragments arranged by size:
3274 1327 1068 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 3554 1166
Size: 2388 4258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2522 1184
Size: 1338 5308
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2109 2141 2109
Size: 32 6614
AlwNI CAG_nnn'CTG
Cuts at: 3679 4389 5961 3679
Size: 710 1572 4364
Fragments arranged by size:
4364 1572 710
ApaLI G'TGCA_C
Cuts at: 4703 5859 4703
Size: 1156 5490
ApoI r'AATT_y
Cuts at: 1493 2219 3955 4181 4333 6442 1493
Size: 726 1736 226 152 2109 1697
Fragments arranged by size:
2109 1736 1697 726 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 2112 2378 3920 3971 1870
Size: 73 97 72 266 1542 51 4545
Fragments arranged by size:
4545 1542 266 97 73 72 51
BamHI G'GATC_C
Cuts at: 3306 3976 4343 3306
Size: 670 367 5609
Fragments arranged by size:
5609 670 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2845 2870 3139 3326
Size: 325 217 627 305 753 25 269 187
Cuts at: 3326 3902 3967 5422 618
Size: 576 65 1455 1842
Fragments arranged by size:
1842 1455 753 627 576 325 305 269
217 187 65 25
BanII G_rGCy'C
Cuts at: 1391 3495 3965 1391
Size: 2104 470 4072
Fragments arranged by size:
4072 2104 470
BbeI G_GCGC'C
Cuts at: 2874 2874
Size: 6646
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3470 3552 3585 928
Size: 971 1571 82 33 3989
Fragments arranged by size:
3989 1571 971 82 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3148 3182 4837 4871 1903
Size: 34 1211 34 1655 34 3678
Fragments arranged by size:
3678 1655 1211 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4538 5748 4538
Size: 1210 5436
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5257 136
Size: 107 122 71 631 605 337 3248 1525
Fragments arranged by size:
3248 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3933 6641 3933
Size: 2708 3938
BlpI GC'TnA_GC
Cuts at: 4063 4063
Size: 6646
Bme1580I G_kGCm'C
Cuts at: 1790 2850 3056 3144 4707 5863 1790
Size: 1060 206 88 1563 1156 2573
Fragments arranged by size:
2573 1563 1156 1060 206 88
BmgBI CAC'GTC
Cuts at: 1318 2809 1318
Size: 1491 5155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4377 5335 442
Size: 416 53 83 399 24 2960 958 1753
Fragments arranged by size:
2960 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2905 3712 3877 5306 1239
Size: 1666 807 165 1429 2579
Fragments arranged by size:
2579 1666 1429 807 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 2945 2959 3389 4771 5636 5934 2641
Size: 304 14 430 1382 865 298 3353
Fragments arranged by size:
3353 1382 865 430 304 298 14
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2615 5309 882
Size: 617 1116 2694 2219
Fragments arranged by size:
2694 2219 1116 617
BsaAI yAC'GTr
Cuts at: 493 1204 2025 4437 493
Size: 711 821 2412 2702
Fragments arranged by size:
2702 2412 821 711
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2871 3192 4453 4835
Size: 53 83 186 936 1338 321 1261 382
Cuts at: 4835 5470 275
Size: 635 1451
Fragments arranged by size:
1451 1338 1261 936 635 382 321 186
83 53
BsaWI w'CCGG_w
Cuts at: 5079 5477 5751 5898 6309 5079
Size: 398 274 147 411 5416
Fragments arranged by size:
5416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2109 2139 3123 3153 1178
Size: 30 165 30 706 30 984 30 4671
Fragments arranged by size:
4671 984 706 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 2471 2981 1186
Size: 944 341 510 4851
Fragments arranged by size:
4851 944 510 341
BseYI C'CCAG_C
Cuts at: 2073 2200 2482 2765 2980 3143 3564 5849 6345
Size: 127 282 283 215 163 421 2285 496
Cuts at: 6345 2073
Size: 2374
Fragments arranged by size:
2374 2285 496 421 283 282 215 163
127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2628 3434 1163
Size: 573 892 806 4375
Fragments arranged by size:
4375 892 806 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2260 4096 4857 5885 1540
Size: 275 186 259 1836 761 1028 2301
Fragments arranged by size:
2301 1836 1028 761 275 259 186
BsiHKAI G_wGCw'C
Cuts at: 2552 3283 3495 4707 4792 5863 2552
Size: 731 212 1212 85 1071 3335
Fragments arranged by size:
3335 1212 1071 731 212 85
BsmI GAATG_Cn'
Cuts at: 2437 4157 4250 2437
Size: 1720 93 4833
Fragments arranged by size:
4833 1720 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3526 3526
Size: 6646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2552 2850 3056 3144 3283 3495 3965
Size: 399 762 298 206 88 139 212 470
Cuts at: 3965 4707 4792 5863 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 762 742 470 399 298 212
206 139 88 85
BspEI T'CCGG_A
Cuts at: 6309 6309
Size: 6646
BspHI T'CATG_A
Cuts at: 2793 4535 6265 2793
Size: 1742 1730 3174
Fragments arranged by size:
3174 1742 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2640 4001 844
Size: 1796 1361 3489
Fragments arranged by size:
3489 1796 1361
BsrBI CCG'CTC
Cuts at: 1394 1823 2145 3726 4533 1394
Size: 429 322 1581 807 3507
Fragments arranged by size:
3507 1581 807 429 322
BsrDI GCAATG_nn'
Cuts at: 65 1248 2314 3150 3201 5141 5315 65
Size: 1183 1066 836 51 1940 174 1396
Fragments arranged by size:
1940 1396 1183 1066 836 174 51
BsrFI r'CCGG_y
Cuts at: 1700 1810 2579 3872 5290 5477 1700
Size: 110 769 1293 1418 187 2869
Fragments arranged by size:
2869 1418 1293 769 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 2545 4706 5718 2545
Size: 2161 1012 3473
Fragments arranged by size:
3473 2161 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2083 3292 794
Size: 1289 1209 4148
Fragments arranged by size:
4148 1289 1209
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6646
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 2617
Size: 6646
BstYI r'GATC_y
Cuts at: 1972 3306 3933 3976 4343 4728 4745 6197 6283
Size: 1334 627 43 367 385 17 1452 86
Cuts at: 6283 6295 6641 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1334 627 385 367 346 86
43 17 12
BstZ17I GTA'TAC
Cuts at: 3099 3099
Size: 6646
Bsu36I CC'TnA_GG
Cuts at: 3884 3884
Size: 6646
BtgI C'CryG_G
Cuts at: 513 1065 2004 2321 2635 3006 3661 513
Size: 552 939 317 314 371 655 3498
Fragments arranged by size:
3498 939 655 552 371 317 314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2575 2590 487
Size: 683 56 1349 15 4543
Fragments arranged by size:
4543 1349 683 56 15
BtsI GCAGTG_nn'
Cuts at: 2058 3388 3645 4162 4956 4976 2058
Size: 1330 257 517 794 20 3728
Fragments arranged by size:
3728 1330 794 517 257 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6611
DraI TTT'AAA
Cuts at: 3950 3950
Size: 6646
DraIII CAC_nnn'GTG
Cuts at: 3188 3188
Size: 6646
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2814 3009
Size: 54 1425 50 275 18 189 795 195
Cuts at: 3009 3532 4093 4982 6611 8
Size: 523 561 889 1629 43
Fragments arranged by size:
1629 1425 889 795 561 523 275 195
189 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4093 1537
Size: 275 2281 4090
Fragments arranged by size:
4090 2281 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 2400 3920 4576 1353
Size: 993 54 1520 656 3423
Fragments arranged by size:
3423 1520 993 656 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2267 3620 5233 5607 5753 387
Size: 1064 138 678 1353 1613 374 146 1280
Fragments arranged by size:
1613 1353 1280 1064 678 374 146 138
EcoICRI GAG'CTC
Cuts at: 3493 3493
Size: 6646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2905 3484 3712 3877 4709 5306 6093
Size: 393 1273 579 228 165 832 597 787
Cuts at: 6093 6361 1239
Size: 268 1524
Fragments arranged by size:
1524 1273 832 787 597 579 393 268
228 165
EcoNI CCTnn'n_nnAGG
Cuts at: 2447 2447
Size: 6646
EcoO109I rG'GnC_Cy
Cuts at: 2652 3364 3575 2652
Size: 712 211 5723
Fragments arranged by size:
5723 712 211
EcoRI G'AATT_C
Cuts at: 2219 3955 4333 2219
Size: 1736 378 4532
Fragments arranged by size:
4532 1736 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 3085 3313 2239
Size: 846 228 5572
Fragments arranged by size:
5572 846 228
FspI TGC'GCA
Cuts at: 3701 5152 3701
Size: 1451 5195
HaeII r_GCGC'y
Cuts at: 1678 2155 2443 2874 3277 3622 5793 1678
Size: 477 288 431 403 345 2171 2531
Fragments arranged by size:
2531 2171 477 431 403 345 288
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 2849 2881 5287
Size: 32 135 32 650 32 595 32 2406
Cuts at: 5287 5319 5361 5393 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2406 650 595 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3990 4236 669
Size: 988 281 2052 246 3079
Fragments arranged by size:
3079 2052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KasI G'GCGC_C
Cuts at: 2870 2870
Size: 6646
KpnI G_GTAC'C
Cuts at: 3330 3906 3971 3330
Size: 576 65 6005
Fragments arranged by size:
6005 576 65
MfeI C'AATT_G
Cuts at: 4245 4245
Size: 6646
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3255 5760 5944 1305
Size: 1950 2505 184 2007
Fragments arranged by size:
2505 2007 1950 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2021 2816 3011 10
Size: 54 1425 343 189 795 195 3645
Fragments arranged by size:
3645 1425 795 343 195 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2594 2807 3032 3554 4604 4963 5122
Size: 648 1428 213 225 522 1050 359 159
Cuts at: 5122 518
Size: 2042
Fragments arranged by size:
2042 1428 1050 648 522 359 225 213
159
MspA1I CmG'CkG
Cuts at: 1606 2515 2637 3262 3378 4062 4739 5887 6132
Size: 909 122 625 116 684 677 1148 245
Cuts at: 6132 1606
Size: 2120
Fragments arranged by size:
2120 1148 909 684 677 625 245 122
116
NaeI GCC'GGC
Cuts at: 3874 3874
Size: 6646
NarI GG'CG_CC
Cuts at: 2871 2871
Size: 6646
NcoI C'CATG_G
Cuts at: 513 1065 2004 3006 513
Size: 552 939 1002 4153
Fragments arranged by size:
4153 1002 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6646
NgoMIV G'CCGG_C
Cuts at: 3872 3872
Size: 6646
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NruI TCG'CGA
Cuts at: 2262 2262
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 2275 3673 3688 4004 5549 1132
Size: 97 1046 1398 15 316 1545 2229
Fragments arranged by size:
2229 1545 1398 1046 316 97 15
PasI CC'CwG_GG
Cuts at: 1348 3511 1348
Size: 2163 4483
PciI A'CATG_T
Cuts at: 2271 5545 2271
Size: 3274 3372
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3571 1108
Size: 2463 4183
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PmlI CAC'GTG
Cuts at: 2025 2025
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2109 2141 4715 4747 6228 6260 2109
Size: 32 2574 32 1481 32 2495
Fragments arranged by size:
2574 2495 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2652 3575 2652
Size: 923 5723
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6646
PsiI TTA'TAA
Cuts at: 4216 4216
Size: 6646
PstI C_TGCA'G
Cuts at: 830 3348 3998 830
Size: 2518 650 3478
Fragments arranged by size:
3478 2518 650
PvuI CG_AT'CG
Cuts at: 2001 2260 2001
Size: 259 6387
SacI G_AGCT'C
Cuts at: 3495 3495
Size: 6646
SacII CC_GC'GG
Cuts at: 2638 2638
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SanDI GG'GwC_CC
Cuts at: 3575 3575
Size: 6646
SapI GCTCTTCn'nnn_
Cuts at: 2346 2346
Size: 6646
SbfI CC_TGCA'GG
Cuts at: 3998 3998
Size: 6646
ScaI AGT'ACT
Cuts at: 2249 4894 6606 2249
Size: 2645 1712 2289
Fragments arranged by size:
2645 2289 1712
SfcI C'TryA_G
Cuts at: 826 1045 3344 3994 5129 5810 6001 826
Size: 219 2299 650 1135 681 191 1471
Fragments arranged by size:
2299 1471 1135 681 650 219 191
SfoI GGC'GCC
Cuts at: 2872 2872
Size: 6646
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3973 1872
Size: 2101 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2112 2656 2938 2960 3368 4750
Size: 197 926 169 544 282 22 408 1382
Cuts at: 4750 5651 5913 820
Size: 901 262 1553
Fragments arranged by size:
1553 1382 926 901 544 408 282 262
197 169 22
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 3673 4004 1132
Size: 2541 331 3774
Fragments arranged by size:
3774 2541 331
SspI AAT'ATT
Cuts at: 5 52 4570 5
Size: 47 4518 2081
Fragments arranged by size:
4518 2081 47
StuI AGG'CCT
Cuts at: 1517 2016 2342 2693 1517
Size: 499 326 351 5470
Fragments arranged by size:
5470 499 351 326
StyI C'CwwG_G
Cuts at: 513 1065 2004 3006 4085 513
Size: 552 939 1002 1079 3074
Fragments arranged by size:
3074 1079 1002 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2433 2590 4673 4690 4843 5028 5473
Size: 1255 853 157 2083 17 153 185 445
Cuts at: 5473 325
Size: 1498
Fragments arranged by size:
2083 1498 1255 853 445 185 157 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 2719 3348
Size: 274 80 33 51 1443 270 472 629
Cuts at: 3348 4892 6604 96
Size: 1544 1712 138
Fragments arranged by size:
1712 1544 1443 629 472 274 270 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 6606 6638 3123
Size: 32 3451 32 3131
Fragments arranged by size:
3451 3131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2861 1316
Size: 1545 5101
XbaI T'CTAG_A
Cuts at: 3457 3982 3457
Size: 525 6121
XhoI C'TCGA_G
Cuts at: 1943 2112 1943
Size: 169 6477
XmaI C'CCGG_G
Cuts at: 1870 3971 1870
Size: 2101 4545
XmnI GAAnn'nnTTC
Cuts at: 2219 4775 2219
Size: 2556 4090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI EcoRV FseI FspAI NsiI PacI
PfoI PspOMI PspXI PsrI PvuII RsrII SexAI SfiI
SrfI SwaI XcmI