Restriction map for
pFN21AB6159
(Circular) (Six-base) MAPSORT of: pFN21AB6159.seq Check: 2587 from: 1 to: 6502
pFN21AB6159 6502 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2872 3845 1937
Size: 935 973 4594
Fragments arranged by size:
4594 973 935
Acc65I G'GTAC_C
Cuts at: 2182 3823 2182
Size: 1641 4861
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2375 4565 5949 6217 1632
Size: 375 368 2190 1384 268 1917
Fragments arranged by size:
2190 1917 1384 375 368 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 2383 3651 3799 5401 6378 1203
Size: 1180 1268 148 1602 977 1327
Fragments arranged by size:
1602 1327 1268 1180 977 148
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 3132 1166
Size: 1966 4536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6502
AlwNI CAG_nnn'CTG
Cuts at: 4245 5817 4245
Size: 1572 4930
ApaLI G'TGCA_C
Cuts at: 4559 5715 4559
Size: 1156 5346
ApoI r'AATT_y
Cuts at: 1493 2437 3811 4037 4189 6298 1493
Size: 944 1374 226 152 2109 1697
Fragments arranged by size:
2109 1697 1374 944 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 2782 3827 1870
Size: 73 839 1045 4545
Fragments arranged by size:
4545 1045 839 73
BamHI G'GATC_C
Cuts at: 3832 4199 3832
Size: 367 6135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 3823 5278 618
Size: 325 217 627 395 1641 1455 1842
Fragments arranged by size:
1842 1641 1455 627 395 325 217
BanII G_rGCy'C
Cuts at: 1391 2649 3821 1391
Size: 1258 1172 4072
Fragments arranged by size:
4072 1258 1172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5531
BbvCI CC'TCA_GC
Cuts at: 2939 2939
Size: 6502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2107 2141 4693 4727 1903
Size: 34 170 34 2552 34 3678
Fragments arranged by size:
3678 2552 170 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3201 4394 5604 3201
Size: 1193 1210 4099
Fragments arranged by size:
4099 1210 1193
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5113 136
Size: 107 122 71 631 605 3441 1525
Fragments arranged by size:
3441 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2394 3271 6497 2394
Size: 877 3226 2399
Fragments arranged by size:
3226 2399 877
BlpI GC'TnA_GC
Cuts at: 2653 3919 2653
Size: 1266 5236
Bme1580I G_kGCm'C
Cuts at: 1790 4563 5719 1790
Size: 2773 1156 2573
Fragments arranged by size:
2773 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2039 2053 4233
Size: 416 53 83 399 24 622 14 2180
Cuts at: 4233 5191 442
Size: 958 1753
Fragments arranged by size:
2180 1753 958 622 416 399 83 53
24 14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 6502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 3176 3405 5162 1239
Size: 1061 876 229 1757 2579
Fragments arranged by size:
2579 1757 1061 876 229
Bpu10I CC'TnA_GC
Cuts at: 2939 2939
Size: 6502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2557 2731 4627 5492 5790 2557
Size: 174 1896 865 298 3269
Fragments arranged by size:
3269 1896 865 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5165 882
Size: 617 3666 2219
Fragments arranged by size:
3666 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3652 4293 493
Size: 711 2448 641 2702
Fragments arranged by size:
2702 2448 711 641
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4309 4691 5326 275
Size: 53 83 186 936 2776 382 635 1451
Fragments arranged by size:
2776 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2746 3131 4935 5333 5607 5754 6165 2746
Size: 385 1804 398 274 147 411 3083
Fragments arranged by size:
3083 1804 411 398 385 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6277
Fragments arranged by size:
6277 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2538 2573 3065 3431 3683 1186
Size: 1352 35 492 366 252 4005
Fragments arranged by size:
4005 1352 492 366 252 35
BseYI C'CCAG_C
Cuts at: 2585 2648 5705 6201 2585
Size: 63 3057 496 2886
Fragments arranged by size:
3057 2886 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2433 2696 2920 3004 3676 1163
Size: 573 697 263 224 84 672 3989
Fragments arranged by size:
3989 697 672 573 263 224 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3952 4713 5741 1540
Size: 275 186 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4563 4648 5719 4563
Size: 85 1071 5346
Fragments arranged by size:
5346 1071 85
BsmI GAATG_Cn'
Cuts at: 2579 4013 4106 2579
Size: 1434 93 4975
Fragments arranged by size:
4975 1434 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2649 3821 4563 4648 5719 1391
Size: 399 859 1172 742 85 1071 2174
Fragments arranged by size:
2174 1172 1071 859 742 399 85
BspEI T'CCGG_A
Cuts at: 6165 6165
Size: 6502
BspHI T'CATG_A
Cuts at: 4391 6121 4391
Size: 1730 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3857 844
Size: 3013 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3373 4389 1394
Size: 429 1550 1016 3507
Fragments arranged by size:
3507 1550 1016 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4997 5171 65
Size: 1183 3749 174 1396
Fragments arranged by size:
3749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5146 5333 1700
Size: 110 3336 187 2869
Fragments arranged by size:
3336 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3616 96
Size: 3520 2982
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 3368 4562 5574 3368
Size: 1194 1012 4296
Fragments arranged by size:
4296 1194 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2907 794
Size: 2113 4389
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstXI CCAn_nnnn'nTGG
Cuts at: 2454 2454
Size: 6502
BstYI r'GATC_y
Cuts at: 1972 2394 3271 3832 4199 4584 4601 6053 6139
Size: 422 877 561 367 385 17 1452 86
Cuts at: 6139 6151 6497 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 877 561 422 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2873 2873
Size: 6502
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5763
Fragments arranged by size:
5763 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 2908 3664 4018 4812 4832 2824
Size: 84 756 354 794 20 4494
Fragments arranged by size:
4494 794 756 354 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 2839 3806 2839
Size: 967 5535
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3415 3667 3949
Size: 54 1425 50 275 18 1585 252 282
Cuts at: 3949 4838 6467 8
Size: 889 1629 43
Fragments arranged by size:
1629 1585 1425 889 282 275 252 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 2228 2961 4432 1353
Size: 690 185 733 1471 3423
Fragments arranged by size:
3423 1471 733 690 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2300 2375 3176 3405 4565 5162
Size: 393 375 293 75 801 229 1160 597
Cuts at: 5162 5949 6217 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1160 801 787 597 393 375 293
268 229 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2619 2619
Size: 6502
EcoO109I rG'GnC_Cy
Cuts at: 2787 2787
Size: 6502
EcoRI G'AATT_C
Cuts at: 2437 3811 4189 2437
Size: 1374 378 4750
Fragments arranged by size:
4750 1374 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 6502
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 5649 1678
Size: 3971 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 2227 2259 2838
Size: 32 135 32 520 32 103 32 579
Cuts at: 2838 2870 3086 3118 5143 5175 5217 5249 1373
Size: 32 216 32 2025 32 42 32 2626
Fragments arranged by size:
2626 2025 579 520 216 135 103 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2381 3186 3354 3606 3846 4092
Size: 988 281 443 805 168 252 240 246
Cuts at: 4092 669
Size: 3079
Fragments arranged by size:
3079 988 805 443 281 252 246 240
168
HindIII A'AGCT_T
Cuts at: 748 2833 3001 3169 748
Size: 2085 168 168 4081
Fragments arranged by size:
4081 2085 168 168
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KpnI G_GTAC'C
Cuts at: 2186 3827 2186
Size: 1641 4861
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2958 3410 5616 5800 1305
Size: 1653 452 2206 184 2007
Fragments arranged by size:
2206 2007 1653 452 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4680
Fragments arranged by size:
4680 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3132 3300 3552 4460 4819 4978 518
Size: 648 1966 168 252 908 359 159 2042
Fragments arranged by size:
2042 1966 908 648 359 252 168 159
MspA1I CmG'CkG
Cuts at: 1606 3918 4595 5743 5988 1606
Size: 2312 677 1148 245 2120
Fragments arranged by size:
2312 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5950
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 2387 3860 5405 1132
Size: 97 1158 1473 1545 2229
Fragments arranged by size:
2229 1545 1473 1158 97
PasI CC'CwG_GG
Cuts at: 1348 2447 1348
Size: 1099 5403
PciI A'CATG_T
Cuts at: 2383 5401 2383
Size: 3018 3484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2069 2179 2541 2758 1108
Size: 961 110 362 217 4852
Fragments arranged by size:
4852 961 362 217 110
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 6084 6116 4571
Size: 32 1481 32 4957
Fragments arranged by size:
4957 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2787 2787
Size: 6502
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PstI C_TGCA'G
Cuts at: 830 3491 3854 830
Size: 2661 363 3478
Fragments arranged by size:
3478 2661 363
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SfcI C'TryA_G
Cuts at: 826 1045 2731 3487 3850 4985 5666 5857 826
Size: 219 1686 756 363 1135 681 191 1471
Fragments arranged by size:
1686 1471 1135 756 681 363 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 3829 1872
Size: 1957 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2572 2710 4606 5507 5769 820
Size: 197 926 629 138 1896 901 262 1553
Fragments arranged by size:
1896 1553 926 901 629 262 197 138
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6502
StyI C'CwwG_G
Cuts at: 513 1065 3941 513
Size: 552 2876 3074
Fragments arranged by size:
3074 2876 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4529 4546 4699 4884 5329 325
Size: 1255 2949 17 153 185 445 1498
Fragments arranged by size:
2949 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 3616 4748
Size: 274 80 33 51 1443 409 1230 1132
Cuts at: 4748 6460 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1230 1132 409 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 2241 1316
Size: 925 5577
XbaI T'CTAG_A
Cuts at: 3838 3838
Size: 6502
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 3827 1870
Size: 1957 4545
XmnI GAAnn'nnTTC
Cuts at: 2959 4631 2959
Size: 1672 4830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI