Restriction map for
pFN21AB6158
(Circular) (Six-base) MAPSORT of: pFN21AB6158.seq Check: 9033 from: 1 to: 6643
pFN21AB6158 6643 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2144 4453 278
Size: 53 83 186 936 608 2309 2468
Fragments arranged by size:
2468 2309 936 608 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3986 1937
Size: 2049 4594
Acc65I G'GTAC_C
Cuts at: 3673 3964 3673
Size: 291 6352
AclI AA'CG_TT
Cuts at: 1563 4770 5143 1563
Size: 3207 373 3063
Fragments arranged by size:
3207 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 2819 3240 4706 6090 6358 1632
Size: 989 198 421 1466 1384 268 1917
Fragments arranged by size:
1917 1466 1384 989 421 268 198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6446
AflIII A'CryG_T
Cuts at: 1203 5542 6519 1203
Size: 4339 977 1327
Fragments arranged by size:
4339 1327 977
AgeI A'CCGG_T
Cuts at: 5474 5474
Size: 6643
AhdI GACnn_n'nnGTC
Cuts at: 5372 5372
Size: 6643
AleI CACnn'nnGTG
Cuts at: 1166 3293 1166
Size: 2127 4516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6643
AlwNI CAG_nnn'CTG
Cuts at: 2255 2875 2929 2985 3182 3194 3218 3795 4386
Size: 620 54 56 197 12 24 577 591
Cuts at: 4386 5958 2255
Size: 1572 2940
Fragments arranged by size:
2940 1572 620 591 577 197 56 54
24 12
ApaI G_GGCC'C
Cuts at: 2082 3577 2082
Size: 1495 5148
ApaLI G'TGCA_C
Cuts at: 3515 4700 5856 3515
Size: 1185 1156 4302
Fragments arranged by size:
4302 1185 1156
ApoI r'AATT_y
Cuts at: 1493 2572 3952 4178 4330 6439 1493
Size: 1079 1380 226 152 2109 1697
Fragments arranged by size:
2109 1697 1380 1079 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5197 160
Size: 872 4165 1606
Fragments arranged by size:
4165 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2150 2901 3570 3968 1870
Size: 73 138 69 751 669 398 4545
Fragments arranged by size:
4545 751 669 398 138 73 69
BamHI G'GATC_C
Cuts at: 3973 4340 3973
Size: 367 6276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2154 2669 3673 3964 5419
Size: 325 217 627 367 515 1004 291 1455
Cuts at: 5419 618
Size: 1842
Fragments arranged by size:
1842 1455 1004 627 515 367 325 291
217
BanII G_rGCy'C
Cuts at: 1391 2082 2427 2777 3577 3804 3962 1391
Size: 691 345 350 800 227 158 4072
Fragments arranged by size:
4072 800 691 350 345 227 158
BbeI G_GCGC'C
Cuts at: 2158 2158
Size: 6643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2663 3228 928
Size: 971 764 565 4343
Fragments arranged by size:
4343 971 764 565
BbvCI CC'TCA_GC
Cuts at: 3585 3585
Size: 6643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2083 2117 4834 4868 1903
Size: 34 146 34 2717 34 3678
Fragments arranged by size:
3678 2717 146 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3605 4535 5745 3605
Size: 930 1210 4503
Fragments arranged by size:
4503 1210 930
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2866 3060 3368
Size: 107 122 71 631 605 1194 194 308
Cuts at: 3368 3497 5254 136
Size: 129 1757 1525
Fragments arranged by size:
1757 1525 1194 631 605 308 194 129
122 107 71
BglII A'GATC_T
Cuts at: 3646 6638 3646
Size: 2992 3651
BlpI GC'TnA_GC
Cuts at: 4060 4060
Size: 6643
Bme1580I G_kGCm'C
Cuts at: 1790 2082 3519 3570 3577 4704 5860 1790
Size: 292 1437 51 7 1127 1156 2573
Fragments arranged by size:
2573 1437 1156 1127 292 51 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2750 3648 4374
Size: 416 53 83 399 24 1333 898 726
Cuts at: 4374 5332 442
Size: 958 1753
Fragments arranged by size:
1753 1333 958 898 726 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2673 3156 3867 5303 1239
Size: 899 535 483 711 1436 2579
Fragments arranged by size:
2579 1436 899 711 535 483
Bpu10I CC'TnA_GC
Cuts at: 2112 3585 2112
Size: 1473 5170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2546 4768 5633 5931 2507
Size: 39 2222 865 298 3219
Fragments arranged by size:
3219 2222 865 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2343 2898 3112 5306 882
Size: 617 844 555 214 2194 2219
Fragments arranged by size:
2219 2194 844 617 555 214
BsaAI yAC'GTr
Cuts at: 493 1204 4434 493
Size: 711 3230 2702
Fragments arranged by size:
3230 2702 711
BsaBI GATnn'nnATC
Cuts at: 6637 6637
Size: 6643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 2155 4450 4832
Size: 53 83 186 936 608 14 2295 382
Cuts at: 4832 5467 275
Size: 635 1451
Fragments arranged by size:
2295 1451 936 635 608 382 186 83
53 14
BsaWI w'CCGG_w
Cuts at: 2641 3110 5076 5474 5748 5895 6306 2641
Size: 469 1966 398 274 147 411 2978
Fragments arranged by size:
2978 1966 469 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3596 3626 1178
Size: 30 165 30 2193 30 4195
Fragments arranged by size:
4195 2193 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3713 1186
Size: 2527 4116
BseYI C'CCAG_C
Cuts at: 3019 3187 3343 3372 3589 3795 3871 3937 5846
Size: 168 156 29 217 206 76 66 1909
Cuts at: 5846 6342 3019
Size: 496 3320
Fragments arranged by size:
3320 1909 496 217 206 168 156 76
66 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 1163
Size: 573 591 5479
Fragments arranged by size:
5479 591 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 2076 2182 2968 4093 4854
Size: 275 186 54 21 106 786 1125 761
Cuts at: 4854 5882 1540
Size: 1028 2301
Fragments arranged by size:
2301 1125 1028 786 761 275 186 106
54 21
BsiHKAI G_wGCw'C
Cuts at: 2427 3316 3468 3519 4704 4789 5860 2427
Size: 889 152 51 1185 85 1071 3210
Fragments arranged by size:
3210 1185 1071 889 152 85 51
BsmI GAATG_Cn'
Cuts at: 4154 4247 4154
Size: 93 6550
BsmBI CGTCTCn'nnnn_
Cuts at: 2780 2798 2780
Size: 18 6625
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2427 2777 3316 3468 3519 3570
Size: 399 292 345 350 539 152 51 51
Cuts at: 3570 3577 3804 3962 4704 4789 5860 1391
Size: 7 227 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 539 399 350 345 292
227 158 152 85 51 51 7
BspEI T'CCGG_A
Cuts at: 2641 6306 2641
Size: 3665 2978
BspHI T'CATG_A
Cuts at: 3633 4532 6262 3633
Size: 899 1730 4014
Fragments arranged by size:
4014 1730 899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 2508 2815 3685 3897 3998 844
Size: 1517 147 307 870 212 101 3489
Fragments arranged by size:
3489 1517 870 307 212 147 101
BsrBI CCG'CTC
Cuts at: 1394 1823 2066 3123 4530 1394
Size: 429 243 1057 1407 3507
Fragments arranged by size:
3507 1407 1057 429 243
BsrDI GCAATG_nn'
Cuts at: 65 1248 5138 5312 65
Size: 1183 3890 174 1396
Fragments arranged by size:
3890 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 5287 5474 1700
Size: 110 208 3269 187 2869
Fragments arranged by size:
3269 2869 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 3985
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6643
BssSI C'ACGA_G
Cuts at: 4703 5715 4703
Size: 1012 5631
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6643
BstBI TT'CG_AA
Cuts at: 5447 5447
Size: 6643
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6643
BstXI CCAn_nnnn'nTGG
Cuts at: 2683 3295 2683
Size: 612 6031
BstYI r'GATC_y
Cuts at: 1972 2322 2508 2925 3646 3973 4340 4725 4742
Size: 350 186 417 721 327 367 385 17
Cuts at: 4742 6194 6280 6292 6638 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 721 417 385 367 350 346
327 186 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2721 2721
Size: 6643
BtgI C'CryG_G
Cuts at: 513 1065 2004 2713 3235 3555 513
Size: 552 939 709 522 320 3601
Fragments arranged by size:
3601 939 709 552 522 320
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5904
Fragments arranged by size:
5904 683 56
BtsI GCAGTG_nn'
Cuts at: 2855 3259 3704 4159 4953 4973 2855
Size: 404 445 455 794 20 4525
Fragments arranged by size:
4525 794 455 445 404 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6608
DraI TTT'AAA
Cuts at: 3947 3947
Size: 6643
DraIII CAC_nnn'GTG
Cuts at: 2371 3236 2371
Size: 865 5778
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5650 809
Size: 507 4334 1802
Fragments arranged by size:
4334 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2073 2127
Size: 54 1425 50 275 18 222 21 54
Cuts at: 2127 2179 2877 3145 3285 3498 4090 4979 6608
Size: 52 698 268 140 213 592 889 1629
Cuts at: 6608 8
Size: 43
Fragments arranged by size:
1629 1425 889 698 592 275 268 222
213 140 54 54 52 50 43 21
18
EagI C'GGCC_G
Cuts at: 1537 1812 2052 2073 2179 4090 1537
Size: 275 240 21 106 1911 4090
Fragments arranged by size:
4090 1911 275 240 106 21
EarI CTCTTCn'nnn_
Cuts at: 1353 3068 3473 3657 4573 1353
Size: 1715 405 184 916 3423
Fragments arranged by size:
3423 1715 916 405 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2741 3672 5230 5604 5750 387
Size: 1064 138 1152 931 1558 374 146 1280
Fragments arranged by size:
1558 1280 1152 1064 931 374 146 138
EcoICRI GAG'CTC
Cuts at: 2425 2425
Size: 6643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2621 2673 2819 3156 3240 3867
Size: 393 506 483 52 146 337 84 627
Cuts at: 3867 4706 5303 6090 6358 1239
Size: 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 627 597 506 483 393
337 268 146 84 52
EcoNI CCTnn'n_nnAGG
Cuts at: 3635 3635
Size: 6643
EcoO109I rG'GnC_Cy
Cuts at: 2557 3574 2557
Size: 1017 5626
EcoRI G'AATT_C
Cuts at: 3952 4330 3952
Size: 378 6265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 3073 3445 2236
Size: 837 372 5434
Fragments arranged by size:
5434 837 372
FspI TGC'GCA
Cuts at: 3134 5149 3134
Size: 2015 4628
FspAI rTGC'GCAy
Cuts at: 3134 3134
Size: 6643
HaeII r_GCGC'y
Cuts at: 1678 2158 2265 5790 1678
Size: 480 107 3525 2531
Fragments arranged by size:
3525 2531 480 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2720 2752 2897 2929 2961
Size: 32 135 32 1148 32 145 32 32
Cuts at: 2961 2993 3067 3092 3099 3124 5284 5316 5358
Size: 32 74 25 7 25 2160 32 42
Cuts at: 5358 5390 1373
Size: 32 2626
Fragments arranged by size:
2626 2160 1148 145 135 74 42 32
32 32 32 32 32 32 32 25
25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3409 3987 4233 669
Size: 988 281 1471 578 246 3079
Fragments arranged by size:
3079 1471 988 578 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6643
HpaI GTT'AAC
Cuts at: 4233 4233
Size: 6643
KasI G'GCGC_C
Cuts at: 2154 2154
Size: 6643
KpnI G_GTAC'C
Cuts at: 3677 3968 3677
Size: 291 6352
MfeI C'AATT_G
Cuts at: 4242 4242
Size: 6643
MluI A'CGCG_T
Cuts at: 6519 6519
Size: 6643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2457 5757 5941 1305
Size: 1152 3300 184 2007
Fragments arranged by size:
3300 2007 1152 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3147 3287 10
Size: 54 1425 343 1315 140 3366
Fragments arranged by size:
3366 1425 1315 343 140 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3153 3293 3485 4601 4960 5119 518
Size: 648 1987 140 192 1116 359 159 2042
Fragments arranged by size:
2042 1987 1116 648 359 192 159 140
MspA1I CmG'CkG
Cuts at: 1606 2178 2229 2814 2985 3347 3589 3871 4059
Size: 572 51 585 171 362 242 282 188
Cuts at: 4059 4736 5884 6129 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 585 572 362 282 245
242 188 171 51
NarI GG'CG_CC
Cuts at: 2155 2155
Size: 6643
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5152
Fragments arranged by size:
5152 939 552
NdeI CA'TA_TG
Cuts at: 387 3486 387
Size: 3099 3544
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6643
NotI GC'GGCC_GC
Cuts at: 2052 2179 4090 2052
Size: 127 1911 4605
Fragments arranged by size:
4605 1911 127
NspI r_CATG'y
Cuts at: 1132 1229 4001 5546 1132
Size: 97 2772 1545 2229
Fragments arranged by size:
2772 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6643
PciI A'CATG_T
Cuts at: 5542 5542
Size: 6643
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6643
PfoI T'CCnGG_A
Cuts at: 3924 3924
Size: 6643
PmeI GTTT'AAAC
Cuts at: 3947 3947
Size: 6643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4712 4744 6225 6257 4712
Size: 32 1481 32 5098
Fragments arranged by size:
5098 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2557 2557
Size: 6643
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6643
PsiI TTA'TAA
Cuts at: 4213 4213
Size: 6643
PspOMI G'GGCC_C
Cuts at: 2078 3573 2078
Size: 1495 5148
PstI C_TGCA'G
Cuts at: 830 2358 2432 3582 3699 3995 830
Size: 1528 74 1150 117 296 3478
Fragments arranged by size:
3478 1528 1150 296 117 74
PvuI CG_AT'CG
Cuts at: 2001 2968 2001
Size: 967 5676
PvuII CAG'CTG
Cuts at: 2985 3589 3871 2985
Size: 604 282 5757
Fragments arranged by size:
5757 604 282
SacI G_AGCT'C
Cuts at: 2427 2427
Size: 6643
SalI G'TCGA_C
Cuts at: 1936 3985 1936
Size: 2049 4594
SapI GCTCTTCn'nnn_
Cuts at: 3473 3473
Size: 6643
SbfI CC_TGCA'GG
Cuts at: 3995 3995
Size: 6643
ScaI AGT'ACT
Cuts at: 2528 4891 6603 2528
Size: 2363 1712 2568
Fragments arranged by size:
2568 2363 1712
SfcI C'TryA_G
Cuts at: 826 1045 2354 2428 3578 3695 3991 5126 5807
Size: 219 1309 74 1150 117 296 1135 681
Cuts at: 5807 5998 826
Size: 191 1471
Fragments arranged by size:
1471 1309 1150 1135 681 296 219 191
117 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 3368 3497 3368
Size: 129 6514
SfoI GGC'GCC
Cuts at: 2156 2156
Size: 6643
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6643
SmaI CCC'GGG
Cuts at: 1872 2083 2152 3572 3970 1872
Size: 211 69 1420 398 4545
Fragments arranged by size:
4545 1420 398 211 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2522 2561 4747 5648 5910 820
Size: 197 926 579 39 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 579 262 197 39
SnaBI TAC'GTA
Cuts at: 493 4434 493
Size: 3941 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6643
SphI G_CATG'C
Cuts at: 1132 4001 1132
Size: 2869 3774
SrfI GCCC'GGGC
Cuts at: 2083 2083
Size: 6643
SspI AAT'ATT
Cuts at: 5 52 4567 5
Size: 47 4515 2081
Fragments arranged by size:
4515 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6643
StyI C'CwwG_G
Cuts at: 513 1065 2004 2949 4082 513
Size: 552 939 945 1133 3074
Fragments arranged by size:
3074 1133 945 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 4670 4687 4840 5025 5470 325
Size: 1255 1968 1122 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 1122 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2526 2754 3842
Size: 274 80 33 51 1443 549 228 1088
Cuts at: 3842 4889 6601 96
Size: 1047 1712 138
Fragments arranged by size:
1712 1443 1088 1047 549 274 228 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6603 6635 6603
Size: 32 6611
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6643
XbaI T'CTAG_A
Cuts at: 3979 3979
Size: 6643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3454 3454
Size: 6643
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6643
XmaI C'CCGG_G
Cuts at: 1870 2081 2150 3570 3968 1870
Size: 211 69 1420 398 4545
Fragments arranged by size:
4545 1420 398 211 69
XmnI GAAnn'nnTTC
Cuts at: 4772 4772
Size: 6643
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2142 4451 276
Size: 53 83 186 936 608 2309 2468
Fragments arranged by size:
2468 2309 936 608 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I EcoRV FseI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SwaI