Restriction map for
pFN21AB6157
(Circular) (Six-base) MAPSORT of: pFN21AB6157.seq Check: 574 from: 1 to: 6499
pFN21AB6157 6499 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3691 3691
Size: 6499
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4309 278
Size: 53 83 186 936 2773 2468
Fragments arranged by size:
2773 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3181 3220 3842 1937
Size: 1244 39 622 4594
Fragments arranged by size:
4594 1244 622 39
Acc65I G'GTAC_C
Cuts at: 3820 3820
Size: 6499
AclI AA'CG_TT
Cuts at: 1563 4626 4999 1563
Size: 3063 373 3063
Fragments arranged by size:
3063 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 3018 3462 4562 5946 6214 1632
Size: 971 415 444 1100 1384 268 1917
Fragments arranged by size:
1917 1384 1100 971 444 415 268
AflII C'TTAA_G
Cuts at: 820 1017 2876 820
Size: 197 1859 4443
Fragments arranged by size:
4443 1859 197
AflIII A'CryG_T
Cuts at: 1203 5398 6375 1203
Size: 4195 977 1327
Fragments arranged by size:
4195 1327 977
AgeI A'CCGG_T
Cuts at: 5330 5330
Size: 6499
AhdI GACnn_n'nnGTC
Cuts at: 3571 5228 3571
Size: 1657 4842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3797 1184
Size: 2613 3886
AlwNI CAG_nnn'CTG
Cuts at: 2026 4242 5814 2026
Size: 2216 1572 2711
Fragments arranged by size:
2711 2216 1572
ApaLI G'TGCA_C
Cuts at: 3290 4556 5712 3290
Size: 1266 1156 4077
Fragments arranged by size:
4077 1266 1156
ApoI r'AATT_y
Cuts at: 1493 2853 3808 4034 4186 6295 1493
Size: 1360 955 226 152 2109 1697
Fragments arranged by size:
2109 1697 1360 955 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5053 160
Size: 872 4021 1606
Fragments arranged by size:
4021 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
AvaI C'yCGr_G
Cuts at: 1870 1943 2819 2842 3342 3824 1870
Size: 73 876 23 500 482 4545
Fragments arranged by size:
4545 876 500 482 73 23
BamHI G'GATC_C
Cuts at: 3829 4196 3829
Size: 367 6132
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3684 3710 3820 5275 618
Size: 325 217 627 1897 26 110 1455 1842
Fragments arranged by size:
1897 1842 1455 627 325 217 110 26
BanII G_rGCy'C
Cuts at: 1391 3381 3770 3818 1391
Size: 1990 389 48 4072
Fragments arranged by size:
4072 1990 389 48
BbeI G_GCGC'C
Cuts at: 3688 3688
Size: 6499
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3207 3558 928
Size: 971 1308 351 3869
Fragments arranged by size:
3869 1308 971 351
BbvCI CC'TCA_GC
Cuts at: 3635 3635
Size: 6499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4690 4724 1903
Size: 34 2753 34 3678
Fragments arranged by size:
3678 2753 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4391 5601 4391
Size: 1210 5289
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6499
BfrBI ATG'CAT
Cuts at: 3774 3774
Size: 6499
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2775 5110 136
Size: 107 122 71 631 605 1103 2335 1525
Fragments arranged by size:
2335 1525 1103 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2043 3427 6494 2043
Size: 1384 3067 2048
Fragments arranged by size:
3067 2048 1384
BlpI GC'TnA_GC
Cuts at: 2530 3156 3916 2530
Size: 626 760 5113
Fragments arranged by size:
5113 760 626
Bme1580I G_kGCm'C
Cuts at: 1790 3294 4560 5716 1790
Size: 1504 1266 1156 2573
Fragments arranged by size:
2573 1504 1266 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2622 4230 5188
Size: 416 53 83 399 24 1205 1608 958
Cuts at: 5188 442
Size: 1753
Fragments arranged by size:
1753 1608 1205 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2738 1056
Size: 1682 4817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5159 1239
Size: 3920 2579
Bpu10I CC'TnA_GC
Cuts at: 3635 3635
Size: 6499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2681 3485 4624 5489 5787 2681
Size: 804 1139 865 298 3393
Fragments arranged by size:
3393 1139 865 804 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2820 5162 882
Size: 617 1321 2342 2219
Fragments arranged by size:
2342 2219 1321 617
BsaAI yAC'GTr
Cuts at: 493 1204 3072 4290 493
Size: 711 1868 1218 2702
Fragments arranged by size:
2702 1868 1218 711
BsaBI GATnn'nnATC
Cuts at: 6493 6493
Size: 6499
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4306 4688 5323
Size: 53 83 186 936 2152 621 382 635
Cuts at: 5323 275
Size: 1451
Fragments arranged by size:
2152 1451 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3470 4932 5330 5604 5751 6162 3470
Size: 1462 398 274 147 411 3807
Fragments arranged by size:
3807 1462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2499 2529 1178
Size: 30 165 30 1096 30 5148
Fragments arranged by size:
5148 1096 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2078 2132 2520 2574 2577 3467 3535
Size: 862 30 54 388 54 3 890 68
Cuts at: 3535 1186
Size: 4150
Fragments arranged by size:
4150 890 862 388 68 54 54 30
3
BseYI C'CCAG_C
Cuts at: 2019 2740 2764 3312 5702 6198 2019
Size: 721 24 548 2390 496 2320
Fragments arranged by size:
2390 2320 721 548 496 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2134 3803 1163
Size: 573 398 1669 3859
Fragments arranged by size:
3859 1669 573 398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3949 4710 5738 1540
Size: 275 186 1948 761 1028 2301
Fragments arranged by size:
2301 1948 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2851 3294 4560 4645 5716 2851
Size: 443 1266 85 1071 3634
Fragments arranged by size:
3634 1266 1071 443 85
BsmI GAATG_Cn'
Cuts at: 4010 4103 4010
Size: 93 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2851 3294 3381 3770 3818 4560 4645
Size: 399 1061 443 87 389 48 742 85
Cuts at: 4645 5716 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1061 742 443 399 389 87
85 48
BspEI T'CCGG_A
Cuts at: 3470 6162 3470
Size: 2692 3807
BspHI T'CATG_A
Cuts at: 4388 6118 4388
Size: 1730 4769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2255 3691 3775 3854 844
Size: 1411 1436 84 79 3489
Fragments arranged by size:
3489 1436 1411 84 79
BsrBI CCG'CTC
Cuts at: 1394 1823 4386 1394
Size: 429 2563 3507
Fragments arranged by size:
3507 2563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3774 4994 5168 65
Size: 1183 2526 1220 174 1396
Fragments arranged by size:
2526 1396 1220 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3114 3739 5143 5330 1700
Size: 110 1304 625 1404 187 2869
Fragments arranged by size:
2869 1404 1304 625 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6499
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6499
BssSI C'ACGA_G
Cuts at: 4559 5571 4559
Size: 1012 5487
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6499
BstBI TT'CG_AA
Cuts at: 5303 5303
Size: 6499
BstEII G'GTnAC_C
Cuts at: 1184 2515 1184
Size: 1331 5168
BstYI r'GATC_y
Cuts at: 1972 2043 2184 3427 3829 4196 4581 4598 6050
Size: 71 141 1243 402 367 385 17 1452
Cuts at: 6050 6136 6148 6494 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1243 402 385 367 346 141
86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 3731 3731
Size: 6499
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5947
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5760
Fragments arranged by size:
5760 683 56
BtsI GCAGTG_nn'
Cuts at: 3221 4015 4809 4829 3221
Size: 794 794 20 4891
Fragments arranged by size:
4891 794 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2239 2274 529
Size: 35 1675 35 4754
Fragments arranged by size:
4754 1675 35 35
DraI TTT'AAA
Cuts at: 3803 3803
Size: 6499
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5506 809
Size: 507 4190 1802
Fragments arranged by size:
4190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2260 3116 3558
Size: 54 1425 50 275 18 430 856 442
Cuts at: 3558 3946 4835 6464 8
Size: 388 889 1629 43
Fragments arranged by size:
1629 1425 889 856 442 430 388 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3946 1537
Size: 275 2134 4090
Fragments arranged by size:
4090 2134 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 2994 4429 1353
Size: 960 681 1435 3423
Fragments arranged by size:
3423 1435 960 681
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2005 2045 5086 5460 5606 387
Size: 1064 138 416 40 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 416 374 146 138 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 3018 3462 4562 5159 5946 6214
Size: 393 971 415 444 1100 597 787 268
Cuts at: 6214 1239
Size: 1524
Fragments arranged by size:
1524 1100 971 787 597 444 415 393
268
EcoRI G'AATT_C
Cuts at: 3808 4186 3808
Size: 378 6121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2189 3526 2189
Size: 1337 5162
FspI TGC'GCA
Cuts at: 5005 5005
Size: 6499
HaeII r_GCGC'y
Cuts at: 1678 3688 5646 1678
Size: 2010 1958 2531
Fragments arranged by size:
2531 2010 1958
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3557 3589 5140 5172 5214
Size: 32 135 32 1985 32 1551 32 42
Cuts at: 5214 5246 1373
Size: 32 2626
Fragments arranged by size:
2626 1985 1551 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2418 3843 4089 669
Size: 988 281 480 1425 246 3079
Fragments arranged by size:
3079 1425 988 480 281 246
HindIII A'AGCT_T
Cuts at: 748 2718 748
Size: 1970 4529
HpaI GTT'AAC
Cuts at: 4089 4089
Size: 6499
KasI G'GCGC_C
Cuts at: 3684 3684
Size: 6499
KpnI G_GTAC'C
Cuts at: 3824 3824
Size: 6499
MfeI C'AATT_G
Cuts at: 4098 4098
Size: 6499
MluI A'CGCG_T
Cuts at: 6375 6375
Size: 6499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5613 5797 1305
Size: 4308 184 2007
Fragments arranged by size:
4308 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2262 3560 10
Size: 54 1425 343 430 1298 2949
Fragments arranged by size:
2949 1425 1298 430 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3779 4457 4816 4975 518
Size: 648 2613 678 359 159 2042
Fragments arranged by size:
2613 2042 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 3595 3746 3915 4592 5740 5985 1606
Size: 420 1569 151 169 677 1148 245 2120
Fragments arranged by size:
2120 1569 1148 677 420 245 169 151
NaeI GCC'GGC
Cuts at: 3741 3741
Size: 6499
NarI GG'CG_CC
Cuts at: 3685 3685
Size: 6499
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5947
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6499
NgoMIV G'CCGG_C
Cuts at: 3739 3739
Size: 6499
NheI G'CTAG_C
Cuts at: 1052 2734 1052
Size: 1682 4817
NotI GC'GGCC_GC
Cuts at: 3946 3946
Size: 6499
NsiI A_TGCA'T
Cuts at: 3776 3776
Size: 6499
NspI r_CATG'y
Cuts at: 1132 1229 3857 5402 1132
Size: 97 2628 1545 2229
Fragments arranged by size:
2628 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3680 1348
Size: 2332 4167
PciI A'CATG_T
Cuts at: 5398 5398
Size: 6499
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2026 1108
Size: 918 5581
PfoI T'CCnGG_A
Cuts at: 2896 3147 2896
Size: 251 6248
PmeI GTTT'AAAC
Cuts at: 3803 3803
Size: 6499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4568 4600 6081 6113 4568
Size: 32 1481 32 4954
Fragments arranged by size:
4954 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6499
PsiI TTA'TAA
Cuts at: 4069 4069
Size: 6499
PstI C_TGCA'G
Cuts at: 830 2043 3386 3851 830
Size: 1213 1343 465 3478
Fragments arranged by size:
3478 1343 1213 465
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6499
PvuII CAG'CTG
Cuts at: 2026 3595 2026
Size: 1569 4930
SalI G'TCGA_C
Cuts at: 1936 3841 1936
Size: 1905 4594
SbfI CC_TGCA'GG
Cuts at: 3851 3851
Size: 6499
ScaI AGT'ACT
Cuts at: 4747 6459 4747
Size: 1712 4787
SfcI C'TryA_G
Cuts at: 826 1045 2039 3382 3847 4982 5663 5854 826
Size: 219 994 1343 465 1135 681 191 1471
Fragments arranged by size:
1471 1343 1135 994 681 465 219 191
SfoI GGC'GCC
Cuts at: 3686 3686
Size: 6499
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6499
SmaI CCC'GGG
Cuts at: 1872 3826 1872
Size: 1954 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 2819 2876 3342 3500 4603
Size: 197 926 753 123 57 466 158 1103
Cuts at: 4603 5504 5766 820
Size: 901 262 1553
Fragments arranged by size:
1553 1103 926 901 753 466 262 197
158 123 57
SnaBI TAC'GTA
Cuts at: 493 4290 493
Size: 3797 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6499
SphI G_CATG'C
Cuts at: 1132 3857 1132
Size: 2725 3774
SspI AAT'ATT
Cuts at: 5 52 2885 4423 5
Size: 47 2833 1538 2081
Fragments arranged by size:
2833 2081 1538 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6499
StyI C'CwwG_G
Cuts at: 513 1065 3938 513
Size: 552 2873 3074
Fragments arranged by size:
3074 2873 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4526 4543 4696 4881 5326 325
Size: 1255 2946 17 153 185 445 1498
Fragments arranged by size:
2946 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3361 4745 6457
Size: 274 80 33 51 1443 1384 1384 1712
Cuts at: 6457 96
Size: 138
Fragments arranged by size:
1712 1443 1384 1384 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6459 6491 6459
Size: 32 6467
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6499
XbaI T'CTAG_A
Cuts at: 3835 3835
Size: 6499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3700 3700
Size: 6499
XhoI C'TCGA_G
Cuts at: 1943 2819 3342 1943
Size: 876 523 5100
Fragments arranged by size:
5100 876 523
XmaI C'CCGG_G
Cuts at: 1870 3824 1870
Size: 1954 4545
XmnI GAAnn'nnTTC
Cuts at: 4628 4628
Size: 6499
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4307 276
Size: 53 83 186 936 2773 2468
Fragments arranged by size:
2773 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI