Restriction map for
pFN21AB6155
(Circular) (Six-base) MAPSORT of: pFN21AB6155.seq Check: 6244 from: 1 to: 6496
pFN21AB6155 6496 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3681 4306 278
Size: 53 83 186 936 2145 625 2468
Fragments arranged by size:
2468 2145 936 625 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3839 1937
Size: 1902 4594
Acc65I G'GTAC_C
Cuts at: 3817 3817
Size: 6496
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4559 5943 6211 1632
Size: 2927 1384 268 1917
Fragments arranged by size:
2927 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6299
AflIII A'CryG_T
Cuts at: 1203 5395 6372 1203
Size: 4192 977 1327
Fragments arranged by size:
4192 1327 977
AgeI A'CCGG_T
Cuts at: 5327 5327
Size: 6496
AhdI GACnn_n'nnGTC
Cuts at: 5225 5225
Size: 6496
AleI CACnn'nnGTG
Cuts at: 1166 3359 1166
Size: 2193 4303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3111 1184
Size: 1927 4569
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 2237
Size: 32 6464
AlwNI CAG_nnn'CTG
Cuts at: 2023 2122 2800 3508 4239 5811 2023
Size: 99 678 708 731 1572 2708
Fragments arranged by size:
2708 1572 731 708 678 99
ApaI G_GGCC'C
Cuts at: 2418 2418
Size: 6496
ApaLI G'TGCA_C
Cuts at: 4553 5709 4553
Size: 1156 5340
ApoI r'AATT_y
Cuts at: 1493 3805 4031 4183 6292 1493
Size: 2312 226 152 2109 1697
Fragments arranged by size:
2312 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5050 160
Size: 872 4018 1606
Fragments arranged by size:
4018 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 2294 3549 3821 1870
Size: 73 351 1255 272 4545
Fragments arranged by size:
4545 1255 351 272 73
BamHI G'GATC_C
Cuts at: 3826 4193 3826
Size: 367 6129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2554 2892 3304 3817
Size: 325 217 627 304 463 338 412 513
Cuts at: 3817 5272 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 513 463 412 338 325
304 217
BanII G_rGCy'C
Cuts at: 1391 2015 2160 2418 2570 2610 2717 3030 3243
Size: 624 145 258 152 40 107 313 213
Cuts at: 3243 3815 1391
Size: 572 4072
Fragments arranged by size:
4072 624 572 313 258 213 152 145
107 40
BbeI G_GCGC'C
Cuts at: 2095 2095
Size: 6496
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4687 4721 1903
Size: 34 50 34 2666 34 3678
Fragments arranged by size:
3678 2666 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4388 5598 4388
Size: 1210 5286
BclI T'GATC_A
Cuts at: 1548 3363 1548
Size: 1815 4681
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2046 5107 136
Size: 107 122 71 631 605 374 3061 1525
Fragments arranged by size:
3061 1525 631 605 374 122 107 71
BglII A'GATC_T
Cuts at: 6491 6491
Size: 6496
BlpI GC'TnA_GC
Cuts at: 3913 3913
Size: 6496
Bme1580I G_kGCm'C
Cuts at: 1790 2418 3309 4557 5713 1790
Size: 628 891 1248 1156 2573
Fragments arranged by size:
2573 1248 1156 891 628
BmgBI CAC'GTC
Cuts at: 1318 3789 1318
Size: 2471 4025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2084 3393 3496
Size: 416 53 83 399 24 667 1309 103
Cuts at: 3496 4227 5185 442
Size: 731 958 1753
Fragments arranged by size:
1753 1309 958 731 667 416 399 103
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2245 2999 3017 3086 3530 3743 5156 1239
Size: 1006 754 18 69 444 213 1413 2579
Fragments arranged by size:
2579 1413 1006 754 444 213 69 18
Bpu10I CC'TnA_GC
Cuts at: 2055 2055
Size: 6496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3218 4621 5486 5784 3218
Size: 1403 865 298 3930
Fragments arranged by size:
3930 1403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2190 5159 882
Size: 617 691 2969 2219
Fragments arranged by size:
2969 2219 691 617
BsaAI yAC'GTr
Cuts at: 493 1204 4287 493
Size: 711 3083 2702
Fragments arranged by size:
3083 2702 711
BsaBI GATnn'nnATC
Cuts at: 3698 6490 3698
Size: 2792 3704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2092 3678 4303 4685
Size: 53 83 186 936 559 1586 625 382
Cuts at: 4685 5320 275
Size: 635 1451
Fragments arranged by size:
1586 1451 936 635 625 559 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2238 4929 5327 5601 5748 6159 2238
Size: 2691 398 274 147 411 2575
Fragments arranged by size:
2691 2575 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2597 2627 3482
Size: 30 165 30 834 30 330 30 855
Cuts at: 3482 3512 3584 3614 1178
Size: 30 72 30 4060
Fragments arranged by size:
4060 855 834 330 165 72 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2293 2845 3038 3157 3250 3725 1186
Size: 1107 552 193 119 93 475 3957
Fragments arranged by size:
3957 1107 552 475 193 119 93
BseYI C'CCAG_C
Cuts at: 2410 2558 2800 2874 3595 5699 6195 2410
Size: 148 242 74 721 2104 496 2711
Fragments arranged by size:
2711 2104 721 496 242 148 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2324 3236 3596 1163
Size: 573 399 189 912 360 4063
Fragments arranged by size:
4063 912 573 399 360 189
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3146 3946 4707 5735 1540
Size: 275 186 1145 800 761 1028 2301
Fragments arranged by size:
2301 1145 1028 800 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3030 3075 4557 4642 5713 2031
Size: 999 45 1482 85 1071 2814
Fragments arranged by size:
2814 1482 1071 999 85 45
BsmI GAATG_Cn'
Cuts at: 4007 4100 4007
Size: 93 6403
BsmBI CGTCTCn'nnnn_
Cuts at: 3685 3779 3685
Size: 94 6402
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2031 2160 2418 2570 2610 2717
Size: 399 225 16 129 258 152 40 107
Cuts at: 2717 3030 3075 3243 3309 3815 4557 4642 5713
Size: 313 45 168 66 506 742 85 1071
Cuts at: 5713 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 506 399 313 258 225
168 152 129 107 85 66 45 40
16
BspEI T'CCGG_A
Cuts at: 2238 6159 2238
Size: 3921 2575
BspHI T'CATG_A
Cuts at: 4385 6115 4385
Size: 1730 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2256 3851 844
Size: 1412 1595 3489
Fragments arranged by size:
3489 1595 1412
BsrBI CCG'CTC
Cuts at: 1394 1823 2937 2983 4383 1394
Size: 429 1114 46 1400 3507
Fragments arranged by size:
3507 1400 1114 429 46
BsrDI GCAATG_nn'
Cuts at: 65 1248 4991 5165 65
Size: 1183 3743 174 1396
Fragments arranged by size:
3743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2094 2663 3045 3582 5140 5327 1700
Size: 110 284 569 382 537 1558 187 2869
Fragments arranged by size:
2869 1558 569 537 382 284 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 2863 2897 3203 4556 5568 2863
Size: 34 306 1353 1012 3791
Fragments arranged by size:
3791 1353 1012 306 34
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6496
BstBI TT'CG_AA
Cuts at: 2946 5300 2946
Size: 2354 4142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstYI r'GATC_y
Cuts at: 1972 2499 3198 3826 4193 4578 4595 6047 6133
Size: 527 699 628 367 385 17 1452 86
Cuts at: 6133 6145 6491 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 699 628 527 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2837 513
Size: 552 1772 4172
Fragments arranged by size:
4172 1772 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2409 2902 3145 487
Size: 683 56 1183 493 243 3838
Fragments arranged by size:
3838 1183 683 493 243 56
BtsI GCAGTG_nn'
Cuts at: 2256 4012 4806 4826 2256
Size: 1756 794 20 3926
Fragments arranged by size:
3926 1756 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6461
DraI TTT'AAA
Cuts at: 3800 3800
Size: 6496
DraIII CAC_nnn'GTG
Cuts at: 2899 2899
Size: 6496
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2282 5503 809
Size: 507 966 3221 1802
Fragments arranged by size:
3221 1802 966 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 3143 3943
Size: 54 1425 50 275 18 498 815 800
Cuts at: 3943 4832 6461 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 815 800 498 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3143 3943 1537
Size: 275 1331 800 4090
Fragments arranged by size:
4090 1331 800 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2828 4426 1353
Size: 683 792 1598 3423
Fragments arranged by size:
3423 1598 792 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 5457 5603 387
Size: 1064 138 3494 374 146 1280
Fragments arranged by size:
3494 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3028 3028
Size: 6496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2245 2999 3017 3086 3530 3743 4559
Size: 393 613 754 18 69 444 213 816
Cuts at: 4559 5156 5943 6211 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 816 787 754 613 597 444 393
268 213 69 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2549 3288 2549
Size: 739 5757
EcoO109I rG'GnC_Cy
Cuts at: 2119 2414 3150 3250 3293 3374 2119
Size: 295 736 100 43 81 5241
Fragments arranged by size:
5241 736 295 100 81 43
EcoRI G'AATT_C
Cuts at: 3805 4183 3805
Size: 378 6118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 6496
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 2095 2373 3775 5643 1678
Size: 417 278 1402 1868 2531
Fragments arranged by size:
2531 1868 1402 417 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2062 2094 2597 2629 2840
Size: 32 135 32 490 32 503 32 211
Cuts at: 2840 2872 3393 3425 3584 3616 5137 5169 5211
Size: 32 521 32 159 32 1521 32 42
Cuts at: 5211 5243 1373
Size: 32 2626
Fragments arranged by size:
2626 1521 521 503 490 211 159 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3840 4086 669
Size: 988 281 1902 246 3079
Fragments arranged by size:
3079 1902 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6496
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KasI G'GCGC_C
Cuts at: 2091 2091
Size: 6496
KpnI G_GTAC'C
Cuts at: 3821 3821
Size: 6496
MfeI C'AATT_G
Cuts at: 4095 4095
Size: 6496
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 2673 2787 3102 5610 5794 1305
Size: 741 627 114 315 2508 184 2007
Fragments arranged by size:
2508 2007 741 627 315 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 10
Size: 54 1425 343 498 4176
Fragments arranged by size:
4176 1425 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3359 4454 4813 4972 518
Size: 648 2193 1095 359 159 2042
Fragments arranged by size:
2193 2042 1095 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2581 2785 2839 3095 3465 3674 3912 4589
Size: 975 204 54 256 370 209 238 677
Cuts at: 4589 5737 5982 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 975 677 370 256 245 238
209 204 54
NaeI GCC'GGC
Cuts at: 2096 2096
Size: 6496
NarI GG'CG_CC
Cuts at: 2092 2092
Size: 6496
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5944
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6496
NgoMIV G'CCGG_C
Cuts at: 2094 2094
Size: 6496
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NruI TCG'CGA
Cuts at: 2082 2082
Size: 6496
NspI r_CATG'y
Cuts at: 1132 1229 3854 5399 1132
Size: 97 2625 1545 2229
Fragments arranged by size:
2625 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3284 3427 1348
Size: 1936 143 4417
Fragments arranged by size:
4417 1936 143
PciI A'CATG_T
Cuts at: 5395 5395
Size: 6496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2800 2911 2977 1108
Size: 1692 111 66 4627
Fragments arranged by size:
4627 1692 111 66
PfoI T'CCnGG_A
Cuts at: 2506 2679 3063 3411 3588 3705 2506
Size: 173 384 348 177 117 5297
Fragments arranged by size:
5297 384 348 177 173 117
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2237 2269 4565 4597 6078 6110 2237
Size: 32 2296 32 1481 32 2623
Fragments arranged by size:
2623 2296 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3293 3293
Size: 6496
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6496
PsiI TTA'TAA
Cuts at: 4066 4066
Size: 6496
PspOMI G'GGCC_C
Cuts at: 2414 2414
Size: 6496
PstI C_TGCA'G
Cuts at: 830 2358 2835 3198 3728 3848 830
Size: 1528 477 363 530 120 3478
Fragments arranged by size:
3478 1528 530 477 363 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 3095 3095
Size: 6496
SacI G_AGCT'C
Cuts at: 3030 3030
Size: 6496
SacII CC_GC'GG
Cuts at: 2840 2840
Size: 6496
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SapI GCTCTTCn'nnn_
Cuts at: 2036 2828 2036
Size: 792 5704
SbfI CC_TGCA'GG
Cuts at: 3848 3848
Size: 6496
ScaI AGT'ACT
Cuts at: 4744 6456 4744
Size: 1712 4784
SfcI C'TryA_G
Cuts at: 826 1045 2184 2354 2831 3194 3210 3724 3844
Size: 219 1139 170 477 363 16 514 120
Cuts at: 3844 4979 5660 5851 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1139 1135 681 514 477 363 219
191 170 120 16
SfoI GGC'GCC
Cuts at: 2093 2093
Size: 6496
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 2094 1700
Size: 394 6102
SmaI CCC'GGG
Cuts at: 1872 3551 3823 1872
Size: 1679 272 4545
Fragments arranged by size:
4545 1679 272
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3233 4600 5501 5763 820
Size: 197 926 1290 1367 901 262 1553
Fragments arranged by size:
1553 1367 1290 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6496
SphI G_CATG'C
Cuts at: 1132 3854 1132
Size: 2722 3774
SspI AAT'ATT
Cuts at: 5 52 4420 5
Size: 47 4368 2081
Fragments arranged by size:
4368 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6496
StyI C'CwwG_G
Cuts at: 513 1065 2191 3013 3935 513
Size: 552 1126 822 922 3074
Fragments arranged by size:
3074 1126 922 822 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2572 2653 3304 4523 4540 4693 4878
Size: 1255 992 81 651 1219 17 153 185
Cuts at: 4878 5323 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1219 992 651 445 185 153
81 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4742 6454 96
Size: 274 80 33 51 1443 2765 1712 138
Fragments arranged by size:
2765 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2885 2917 3482 3514 3759 3791 6456 6488 2885
Size: 32 565 32 245 32 2665 32 2893
Fragments arranged by size:
2893 2665 565 245 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2076 1316
Size: 760 5736
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3434 3713 3434
Size: 279 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 3549 3821 1870
Size: 1679 272 4545
Fragments arranged by size:
4545 1679 272
XmnI GAAnn'nnTTC
Cuts at: 2219 2627 4625 2219
Size: 408 1998 4090
Fragments arranged by size:
4090 1998 408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3679 4304 276
Size: 53 83 186 936 2145 625 2468
Fragments arranged by size:
2468 2145 936 625 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NsiI
PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI