Restriction map for
pFN21AB6151
(Circular) (Six-base) MAPSORT of: pFN21AB6151.seq Check: 5247 from: 1 to: 6493
pFN21AB6151 6493 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3836 1937
Size: 1899 4594
Acc65I G'GTAC_C
Cuts at: 3814 3814
Size: 6493
AclI AA'CG_TT
Cuts at: 1563 4620 4993 1563
Size: 3057 373 3063
Fragments arranged by size:
3063 3057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3126 4556 5940 6208 1632
Size: 1494 1430 1384 268 1917
Fragments arranged by size:
1917 1494 1430 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6296
AflIII A'CryG_T
Cuts at: 1203 2043 5392 6369 1203
Size: 840 3349 977 1327
Fragments arranged by size:
3349 1327 977 840
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6493
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2177 2209 2177
Size: 32 6461
AlwNI CAG_nnn'CTG
Cuts at: 3589 4236 5808 3589
Size: 647 1572 4274
Fragments arranged by size:
4274 1572 647
ApaI G_GGCC'C
Cuts at: 3567 3567
Size: 6493
ApaLI G'TGCA_C
Cuts at: 4550 5706 4550
Size: 1156 5337
ApoI r'AATT_y
Cuts at: 1493 2656 2760 2804 2872 3802 4028 4180 6289
Size: 1163 104 44 68 930 226 152 2109
Cuts at: 6289 1493
Size: 1697
Fragments arranged by size:
2109 1697 1163 930 226 152 104 68
44
AseI AT'TA_AT
Cuts at: 160 1032 5047 160
Size: 872 4015 1606
Fragments arranged by size:
4015 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 3818 1870
Size: 73 1875 4545
Fragments arranged by size:
4545 1875 73
BamHI G'GATC_C
Cuts at: 2399 3823 4190 2399
Size: 1424 367 4702
Fragments arranged by size:
4702 1424 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3814 5269 618
Size: 325 217 627 2027 1455 1842
Fragments arranged by size:
2027 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2889 3053 3264 3567 3812 1391
Size: 1498 164 211 303 245 4072
Fragments arranged by size:
4072 1498 303 245 211 164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3114 928
Size: 971 1215 4307
Fragments arranged by size:
4307 1215 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3033 3067 4684 4718 1903
Size: 34 1096 34 1617 34 3678
Fragments arranged by size:
3678 1617 1096 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 2622 3084 3213 1548
Size: 1074 462 129 4828
Fragments arranged by size:
4828 1074 462 129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3526 5104
Size: 107 122 71 631 605 337 1517 1578
Cuts at: 5104 136
Size: 1525
Fragments arranged by size:
1578 1525 1517 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2431 2577 6488 2431
Size: 146 3911 2436
Fragments arranged by size:
3911 2436 146
BlpI GC'TnA_GC
Cuts at: 2936 3910 2936
Size: 974 5519
Bme1580I G_kGCm'C
Cuts at: 1790 3567 4554 5710 1790
Size: 1777 987 1156 2573
Fragments arranged by size:
2573 1777 1156 987
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3016 3694 4224
Size: 416 53 83 399 24 1599 678 530
Cuts at: 4224 5182 442
Size: 958 1753
Fragments arranged by size:
1753 1599 958 678 530 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3317
Size: 6493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 2781 3287 3365 5153 1239
Size: 1280 262 506 78 1788 2579
Fragments arranged by size:
2579 1788 1280 506 262 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4618 5483 5781 4618
Size: 865 298 5330
Fragments arranged by size:
5330 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5156 882
Size: 617 3657 2219
Fragments arranged by size:
3657 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4284 493
Size: 711 3080 2702
Fragments arranged by size:
3080 2702 711
BsaBI GATnn'nnATC
Cuts at: 2451 6487 2451
Size: 4036 2457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4300 4682 5317 275
Size: 53 83 186 936 2767 382 635 1451
Fragments arranged by size:
2767 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4926 5324 5598 5745 6156 4926
Size: 398 274 147 411 5263
Fragments arranged by size:
5263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3131 3161 1178
Size: 30 165 30 1728 30 4510
Fragments arranged by size:
4510 1728 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2432 2897 3554 3590 1186
Size: 1246 465 657 36 4089
Fragments arranged by size:
4089 1246 657 465 36
BseYI C'CCAG_C
Cuts at: 3504 5696 6192 3504
Size: 2192 496 3805
Fragments arranged by size:
3805 2192 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5920
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2029 3943 4704 5732 1540
Size: 275 186 28 1914 761 1028 2301
Fragments arranged by size:
2301 1914 1028 761 275 186 28
BsiHKAI G_wGCw'C
Cuts at: 2298 2889 2937 3324 4554 4639 5710 2298
Size: 591 48 387 1230 85 1071 3081
Fragments arranged by size:
3081 1230 1071 591 387 85 48
BsmI GAATG_Cn'
Cuts at: 2480 2600 2769 2966 4004 4097 2480
Size: 120 169 197 1038 93 4876
Fragments arranged by size:
4876 1038 197 169 120 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2889 2937 3053 3264 3324 3567
Size: 399 508 591 48 116 211 60 243
Cuts at: 3567 3812 4554 4639 5710 1391
Size: 245 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 591 508 399 245 243
211 116 85 60 48
BspEI T'CCGG_A
Cuts at: 6156 6156
Size: 6493
BspHI T'CATG_A
Cuts at: 4382 6112 4382
Size: 1730 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3848 844
Size: 3004 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3487 3580 4380 1394
Size: 429 1664 93 800 3507
Fragments arranged by size:
3507 1664 800 429 93
BsrDI GCAATG_nn'
Cuts at: 65 1248 3729 4988 5162 65
Size: 1183 2481 1259 174 1396
Fragments arranged by size:
2481 1396 1259 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2218 3353 5137 5324 1700
Size: 110 408 1135 1784 187 2869
Fragments arranged by size:
2869 1784 1135 408 187 110
BsrGI T'GTAC_A
Cuts at: 96 3468 96
Size: 3372 3121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 4553 5565 4553
Size: 1012 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3589 794
Size: 2795 3698
BstBI TT'CG_AA
Cuts at: 5297 5297
Size: 6493
BstEII G'GTnAC_C
Cuts at: 1184 2504 1184
Size: 1320 5173
BstXI CCAn_nnnn'nTGG
Cuts at: 2582 2582
Size: 6493
BstYI r'GATC_y
Cuts at: 1972 2383 2399 2431 2452 2577 2898 3823 4190
Size: 411 16 32 21 125 321 925 367
Cuts at: 4190 4575 4592 6044 6130 6142 6488 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 925 411 385 367 346 321
125 86 32 21 17 16 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2463 2847 2994 3129 3450 513
Size: 552 939 459 384 147 135 321 3556
Fragments arranged by size:
3556 939 552 459 384 321 147 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2841 487
Size: 683 56 1615 4139
Fragments arranged by size:
4139 1615 683 56
BtsI GCAGTG_nn'
Cuts at: 3587 4009 4803 4823 3587
Size: 422 794 20 5257
Fragments arranged by size:
5257 794 422 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 3797 3797
Size: 6493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2610 3940
Size: 54 1425 50 275 18 196 584 1330
Cuts at: 3940 4829 6458 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1330 889 584 275 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2026 3940 1537
Size: 275 214 1914 4090
Fragments arranged by size:
4090 1914 275 214
EarI CTCTTCn'nnn_
Cuts at: 1353 2627 4423 1353
Size: 1274 1796 3423
Fragments arranged by size:
3423 1796 1274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3544 5080 5454 5600 387
Size: 1064 138 1955 1536 374 146 1280
Fragments arranged by size:
1955 1536 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2887 2887
Size: 6493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2781 3126 3287 3365 4556 5153
Size: 393 887 262 345 161 78 1191 597
Cuts at: 5153 5940 6208 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1191 887 787 597 393 345 268
262 161 78
EcoNI CCTnn'n_nnAGG
Cuts at: 3347 3347
Size: 6493
EcoO109I rG'GnC_Cy
Cuts at: 2839 3544 3563 3564 2839
Size: 705 19 1 5768
Fragments arranged by size:
5768 705 19 1
EcoRI G'AATT_C
Cuts at: 2656 2872 3802 4180 2656
Size: 216 930 378 4969
Fragments arranged by size:
4969 930 378 216
FseI GG_CCGG'CC
Cuts at: 3357 3357
Size: 6493
FspI TGC'GCA
Cuts at: 2095 4999 2095
Size: 2904 3589
HaeII r_GCGC'y
Cuts at: 1678 5640 1678
Size: 3962 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 3131 3163 3757
Size: 32 135 32 872 32 655 32 594
Cuts at: 3757 3789 5134 5166 5208 5240 1373
Size: 32 1345 32 42 32 2626
Fragments arranged by size:
2626 1345 872 655 594 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 3617 3837 4083 669
Size: 988 281 1234 445 220 246 3079
Fragments arranged by size:
3079 1234 988 445 281 246 220
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6493
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KpnI G_GTAC'C
Cuts at: 3818 3818
Size: 6493
MfeI C'AATT_G
Cuts at: 3072 4092 3072
Size: 1020 5473
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3684 3743 5607 5791 1305
Size: 2379 59 1864 184 2007
Fragments arranged by size:
2379 2007 1864 184 59
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 10
Size: 54 1425 343 780 3891
Fragments arranged by size:
3891 1425 780 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4451 4810 4969 518
Size: 648 3285 359 159 2042
Fragments arranged by size:
3285 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2225 2374 2734 2869 3508 3909 4586 5734
Size: 619 149 360 135 639 401 677 1148
Cuts at: 5734 5979 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 639 619 401 360 245
149 135
NaeI GCC'GGC
Cuts at: 2220 3355 2220
Size: 1135 5358
NcoI C'CATG_G
Cuts at: 513 1065 2004 2463 513
Size: 552 939 459 4543
Fragments arranged by size:
4543 939 552 459
NdeI CA'TA_TG
Cuts at: 387 2740 387
Size: 2353 4140
NgoMIV G'CCGG_C
Cuts at: 2218 3353 2218
Size: 1135 5358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6493
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NruI TCG'CGA
Cuts at: 2067 2067
Size: 6493
NspI r_CATG'y
Cuts at: 1132 1229 3037 3851 5396 1132
Size: 97 1808 814 1545 2229
Fragments arranged by size:
2229 1808 1545 814 97
PasI CC'CwG_GG
Cuts at: 1348 3560 1348
Size: 2212 4281
PciI A'CATG_T
Cuts at: 5392 5392
Size: 6493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2447 1108
Size: 1339 5154
PfoI T'CCnGG_A
Cuts at: 2082 2082
Size: 6493
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4562 4594 6075 6107 4562
Size: 32 1481 32 4948
Fragments arranged by size:
4948 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2839 3544 2839
Size: 705 5788
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6493
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PspOMI G'GGCC_C
Cuts at: 3563 3563
Size: 6493
PstI C_TGCA'G
Cuts at: 830 2868 2881 2965 3606 3845 830
Size: 2038 13 84 641 239 3478
Fragments arranged by size:
3478 2038 641 239 84 13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 2225 2734 2869 3508 2225
Size: 509 135 639 5210
Fragments arranged by size:
5210 639 509 135
SacI G_AGCT'C
Cuts at: 2889 2889
Size: 6493
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 4741 6453 4741
Size: 1712 4781
SfcI C'TryA_G
Cuts at: 826 1045 2864 2877 2961 3602 3841 4976 5657
Size: 219 1819 13 84 641 239 1135 681
Cuts at: 5657 5848 826
Size: 191 1471
Fragments arranged by size:
1819 1471 1135 681 641 239 219 191
84 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 3820 1872
Size: 1948 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4597 5498 5760 820
Size: 197 926 2654 901 262 1553
Fragments arranged by size:
2654 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 3229 152
Size: 3077 3416
SphI G_CATG'C
Cuts at: 1132 3851 1132
Size: 2719 3774
SspI AAT'ATT
Cuts at: 5 52 2664 4417 5
Size: 47 2612 1753 2081
Fragments arranged by size:
2612 2081 1753 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6493
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 2532 3652 3932 513
Size: 552 939 459 69 1120 280 3074
Fragments arranged by size:
3074 1120 939 552 459 280 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2378 4520 4537 4690 4875 5320 325
Size: 1255 798 2142 17 153 185 445 1498
Fragments arranged by size:
2142 1498 1255 798 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 3468 4739
Size: 274 80 33 51 1443 444 1047 1271
Cuts at: 4739 6451 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1271 1047 444 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6453 6485 6453
Size: 32 6461
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6493
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3707 3707
Size: 6493
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6493
XmaI C'CCGG_G
Cuts at: 1870 3818 1870
Size: 1948 4545
XmnI GAAnn'nnTTC
Cuts at: 4622 4622
Size: 6493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FspAI KasI NarI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI