Restriction map for
pFN21AB6149
(Circular) (Six-base) MAPSORT of: pFN21AB6149.seq Check: 9144 from: 1 to: 6493
pFN21AB6149 6493 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2190 2541 3075 3836 1937
Size: 253 351 534 761 4594
Fragments arranged by size:
4594 761 534 351 253
Acc65I G'GTAC_C
Cuts at: 3814 3814
Size: 6493
AclI AA'CG_TT
Cuts at: 1563 2853 4620 4993 1563
Size: 1290 1767 373 3063
Fragments arranged by size:
3063 1767 1290 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2322 3376 3384 3681 4556 5940 6208 1632
Size: 690 1054 8 297 875 1384 268 1917
Fragments arranged by size:
1917 1384 1054 875 690 297 268 8
AflII C'TTAA_G
Cuts at: 820 1017 3664 820
Size: 197 2647 3649
Fragments arranged by size:
3649 2647 197
AflIII A'CryG_T
Cuts at: 1203 5392 6369 1203
Size: 4189 977 1327
Fragments arranged by size:
4189 1327 977
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6493
AlwNI CAG_nnn'CTG
Cuts at: 2705 4236 5808 2705
Size: 1531 1572 3390
Fragments arranged by size:
3390 1572 1531
ApaI G_GGCC'C
Cuts at: 2026 2026
Size: 6493
ApaLI G'TGCA_C
Cuts at: 4550 5706 4550
Size: 1156 5337
ApoI r'AATT_y
Cuts at: 1493 2494 3241 3755 3802 4028 4180 6289 1493
Size: 1001 747 514 47 226 152 2109 1697
Fragments arranged by size:
2109 1697 1001 747 514 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 2816 5047 160
Size: 872 1784 2231 1606
Fragments arranged by size:
2231 1784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 3818 1870
Size: 73 1875 4545
Fragments arranged by size:
4545 1875 73
BamHI G'GATC_C
Cuts at: 3823 4190 3823
Size: 367 6126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3814 5269 618
Size: 325 217 627 2027 1455 1842
Fragments arranged by size:
2027 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2026 2088 3812 1391
Size: 635 62 1724 4072
Fragments arranged by size:
4072 1724 635 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4684 4718 1903
Size: 34 2747 34 3678
Fragments arranged by size:
3678 2747 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 2911 1548
Size: 1363 5130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5104 136
Size: 107 122 71 631 605 3432 1525
Fragments arranged by size:
3432 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6488 6488
Size: 6493
BlpI GC'TnA_GC
Cuts at: 3910 3910
Size: 6493
Bme1580I G_kGCm'C
Cuts at: 1790 2026 2236 4554 5710 1790
Size: 236 210 2318 1156 2573
Fragments arranged by size:
2573 2318 1156 236 210
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3603 4224 5182
Size: 416 53 83 399 24 2186 621 958
Cuts at: 5182 442
Size: 1753
Fragments arranged by size:
2186 1753 958 621 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 3437 3690 5153 1239
Size: 1019 1179 253 1463 2579
Fragments arranged by size:
2579 1463 1179 1019 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3327 4618 5483 5781 3327
Size: 1291 865 298 4039
Fragments arranged by size:
4039 1291 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5156 882
Size: 617 3657 2219
Fragments arranged by size:
3657 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4284 493
Size: 711 3080 2702
Fragments arranged by size:
3080 2702 711
BsaBI GATnn'nnATC
Cuts at: 6487 6487
Size: 6493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4300 4682 5317 275
Size: 53 83 186 936 2767 382 635 1451
Fragments arranged by size:
2767 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2955 4926 5324 5598 5745 6156 2955
Size: 1971 398 274 147 411 3292
Fragments arranged by size:
3292 1971 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2256 2286 1178
Size: 30 165 30 853 30 5385
Fragments arranged by size:
5385 853 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3396 1186
Size: 2210 4283
BseYI C'CCAG_C
Cuts at: 2119 5696 6192 2119
Size: 3577 496 2420
Fragments arranged by size:
3577 2420 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2831 1163
Size: 573 1095 4825
Fragments arranged by size:
4825 1095 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3943 4704 5732 1540
Size: 275 186 1942 761 1028 2301
Fragments arranged by size:
2301 1942 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 4554 4639 5710 2088
Size: 2466 85 1071 2871
Fragments arranged by size:
2871 2466 1071 85
BsmI GAATG_Cn'
Cuts at: 3533 3712 4004 4097 3533
Size: 179 292 93 5929
Fragments arranged by size:
5929 292 179 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2088 2236 3812 4554 4639 5710
Size: 399 236 62 148 1576 742 85 1071
Cuts at: 5710 1391
Size: 2174
Fragments arranged by size:
2174 1576 1071 742 399 236 148 85
62
BspEI T'CCGG_A
Cuts at: 6156 6156
Size: 6493
BspHI T'CATG_A
Cuts at: 4382 6112 4382
Size: 1730 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2853 3848 844
Size: 1226 783 995 3489
Fragments arranged by size:
3489 1226 995 783
BsrBI CCG'CTC
Cuts at: 1394 1823 4380 1394
Size: 429 2557 3507
Fragments arranged by size:
3507 2557 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4988 5162 65
Size: 1183 3740 174 1396
Fragments arranged by size:
3740 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3273 5137 5324 1700
Size: 110 1463 1864 187 2869
Fragments arranged by size:
2869 1864 1463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6493
BssHII G'CGCG_C
Cuts at: 1925 2031 2115 1925
Size: 106 84 6303
Fragments arranged by size:
6303 106 84
BssSI C'ACGA_G
Cuts at: 4553 5565 4553
Size: 1012 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 794
Size: 1256 5237
BstBI TT'CG_AA
Cuts at: 2803 5297 2803
Size: 2494 3999
BstEII G'GTnAC_C
Cuts at: 1184 2255 1184
Size: 1071 5422
BstXI CCAn_nnnn'nTGG
Cuts at: 2793 2793
Size: 6493
BstYI r'GATC_y
Cuts at: 1972 3157 3367 3688 3823 4190 4575 4592 6044
Size: 1185 210 321 135 367 385 17 1452
Cuts at: 6044 6130 6142 6488 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1185 385 367 346 321 210
135 86 17 12
BstZ17I GTA'TAC
Cuts at: 2542 2542
Size: 6493
Bsu36I CC'TnA_GG
Cuts at: 3491 3491
Size: 6493
BtgI C'CryG_G
Cuts at: 513 1065 2004 2026 2403 2943 513
Size: 552 939 22 377 540 4063
Fragments arranged by size:
4063 939 552 540 377 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5754
Fragments arranged by size:
5754 683 56
BtsI GCAGTG_nn'
Cuts at: 2331 2612 2771 4009 4803 4823 2331
Size: 281 159 1238 794 20 4001
Fragments arranged by size:
4001 1238 794 281 159 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 2587 3324 3797 2587
Size: 737 473 5283
Fragments arranged by size:
5283 737 473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3940 4829 6458
Size: 54 1425 50 275 18 2110 889 1629
Cuts at: 6458 8
Size: 43
Fragments arranged by size:
2110 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3940 1537
Size: 275 2128 4090
Fragments arranged by size:
4090 2128 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 2618 3027 3176 3263 4423 1353
Size: 986 279 409 149 87 1160 3423
Fragments arranged by size:
3423 1160 986 409 279 149 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5080 5454 5600 387
Size: 1064 138 3491 374 146 1280
Fragments arranged by size:
3491 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2086 2086
Size: 6493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2322 3376 3384 3437 3681 3690
Size: 393 626 64 1054 8 53 244 9
Cuts at: 3690 4556 5153 5940 6208 1239
Size: 866 597 787 268 1524
Fragments arranged by size:
1524 1054 866 787 626 597 393 268
244 64 53 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2264 2264
Size: 6493
EcoO109I rG'GnC_Cy
Cuts at: 2022 2269 3669 2022
Size: 247 1400 4846
Fragments arranged by size:
4846 1400 247
EcoRI G'AATT_C
Cuts at: 3802 4180 3802
Size: 378 6115
FspI TGC'GCA
Cuts at: 4999 4999
Size: 6493
HaeII r_GCGC'y
Cuts at: 1678 5640 1678
Size: 3962 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2272 2304 5134 5166 5208
Size: 32 135 32 700 32 2830 32 42
Cuts at: 5208 5240 1373
Size: 32 2626
Fragments arranged by size:
2830 2626 700 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3837 4083 669
Size: 988 281 1899 246 3079
Fragments arranged by size:
3079 1899 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3594 748
Size: 2846 3647
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KpnI G_GTAC'C
Cuts at: 3818 3818
Size: 6493
MfeI C'AATT_G
Cuts at: 4092 4092
Size: 6493
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5607 5791 1305
Size: 4302 184 2007
Fragments arranged by size:
4302 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4671
Fragments arranged by size:
4671 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2990 4451 4810 4969 518
Size: 648 1824 1461 359 159 2042
Fragments arranged by size:
2042 1824 1461 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2106 2157 3151 3744 3909 4586 5734
Size: 422 78 51 994 593 165 677 1148
Cuts at: 5734 5979 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 994 677 593 422 245 165
78 51
NcoI C'CATG_G
Cuts at: 513 1065 2004 2943 513
Size: 552 939 939 4063
Fragments arranged by size:
4063 939 939 552
NdeI CA'TA_TG
Cuts at: 387 2569 387
Size: 2182 4311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6493
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NspI r_CATG'y
Cuts at: 1132 1229 3028 3607 3851 5396 1132
Size: 97 1799 579 244 1545 2229
Fragments arranged by size:
2229 1799 1545 579 244 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6493
PciI A'CATG_T
Cuts at: 5392 5392
Size: 6493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 1108
Size: 1173 5320
PfoI T'CCnGG_A
Cuts at: 2274 3414 2274
Size: 1140 5353
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3730 3762 4562 4594 6075 6107 3730
Size: 32 800 32 1481 32 4116
Fragments arranged by size:
4116 1481 800 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2269
Size: 6493
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6493
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PspOMI G'GGCC_C
Cuts at: 2022 2022
Size: 6493
PstI C_TGCA'G
Cuts at: 830 3845 830
Size: 3015 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 2106 2157 3151 3744 2106
Size: 51 994 593 4855
Fragments arranged by size:
4855 994 593 51
SacI G_AGCT'C
Cuts at: 2088 2088
Size: 6493
SacII CC_GC'GG
Cuts at: 2029 2029
Size: 6493
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SapI GCTCTTCn'nnn_
Cuts at: 3263 3263
Size: 6493
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 2445 3340 4741 6453 2445
Size: 895 1401 1712 2485
Fragments arranged by size:
2485 1712 1401 895
SfcI C'TryA_G
Cuts at: 826 1045 3076 3841 4976 5657 5848 826
Size: 219 2031 765 1135 681 191 1471
Fragments arranged by size:
2031 1471 1135 765 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 3820 1872
Size: 1948 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3342 3664 4597 5498 5760 820
Size: 197 926 1399 322 933 901 262 1553
Fragments arranged by size:
1553 1399 933 926 901 322 262 197
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6493
SphI G_CATG'C
Cuts at: 1132 3851 1132
Size: 2719 3774
SspI AAT'ATT
Cuts at: 5 52 4417 5
Size: 47 4365 2081
Fragments arranged by size:
4365 2081 47
StuI AGG'CCT
Cuts at: 1517 3189 3489 1517
Size: 1672 300 4521
Fragments arranged by size:
4521 1672 300
StyI C'CwwG_G
Cuts at: 513 1065 2004 2194 2792 2943 3932 513
Size: 552 939 190 598 151 989 3074
Fragments arranged by size:
3074 989 939 598 552 190 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2682 2857 4520 4537 4690 4875 5320
Size: 1255 1102 175 1663 17 153 185 445
Cuts at: 5320 325
Size: 1498
Fragments arranged by size:
1663 1498 1255 1102 445 185 175 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2443 3036 3338
Size: 274 80 33 51 1443 466 593 302
Cuts at: 3338 3780 4739 6451 96
Size: 442 959 1712 138
Fragments arranged by size:
1712 1443 959 593 466 442 302 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2942 2974 6453 6485 2942
Size: 32 3479 32 2950
Fragments arranged by size:
3479 2950 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2289 1316
Size: 973 5520
XbaI T'CTAG_A
Cuts at: 3211 3829 3211
Size: 618 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2201 2401 2201
Size: 200 6293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6493
XmaI C'CCGG_G
Cuts at: 1870 3818 1870
Size: 1948 4545
XmnI GAAnn'nnTTC
Cuts at: 4622 4622
Size: 6493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI
SfoI SrfI SwaI