Restriction map for
pFN21AB6143
(Circular) (Six-base) MAPSORT of: pFN21AB6143.seq Check: 1417 from: 1 to: 6490
pFN21AB6143 6490 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3833 1937
Size: 1896 4594
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2981 4553 5937 6205 1632
Size: 1349 1572 1384 268 1917
Fragments arranged by size:
1917 1572 1384 1349 268
AfeI AGC'GCT
Cuts at: 3281 3281
Size: 6490
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 3642 5389 6366 1203
Size: 2439 1747 977 1327
Fragments arranged by size:
2439 1747 1327 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 2326 5219 2326
Size: 2893 3597
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AlwNI CAG_nnn'CTG
Cuts at: 2228 2480 4233 5805 2228
Size: 252 1753 1572 2913
Fragments arranged by size:
2913 1753 1572 252
ApaI G_GGCC'C
Cuts at: 2386 2520 3293 2386
Size: 134 773 5583
Fragments arranged by size:
5583 773 134
ApaLI G'TGCA_C
Cuts at: 4547 5703 4547
Size: 1156 5334
ApoI r'AATT_y
Cuts at: 1493 2178 2218 2610 2624 2697 2969 3067 3799
Size: 685 40 392 14 73 272 98 732
Cuts at: 3799 4025 4177 6286 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 732 685 392 272 226 152
98 73 40 14
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 3815 1870
Size: 73 76 1796 4545
Fragments arranged by size:
4545 1796 76 73
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3163 3811 5266 618
Size: 325 217 627 1376 648 1455 1842
Fragments arranged by size:
1842 1455 1376 648 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2386 2407 2520 3293 3809 1391
Size: 995 21 113 773 516 4072
Fragments arranged by size:
4072 995 773 516 113 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2511 928
Size: 971 612 4907
Fragments arranged by size:
4907 971 612
BbvCI CC'TCA_GC
Cuts at: 3758 3770 3758
Size: 12 6478
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3728 3762 4681 4715 1903
Size: 34 1791 34 919 34 3678
Fragments arranged by size:
3678 1791 919 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 5101 136
Size: 107 122 71 631 605 343 3086 1525
Fragments arranged by size:
3086 1525 631 605 343 122 107 71
BglII A'GATC_T
Cuts at: 3379 3457 6485 3379
Size: 78 3028 3384
Fragments arranged by size:
3384 3028 78
BlpI GC'TnA_GC
Cuts at: 2571 3907 2571
Size: 1336 5154
Bme1580I G_kGCm'C
Cuts at: 1790 2386 2520 3293 4551 5707 1790
Size: 596 134 773 1258 1156 2573
Fragments arranged by size:
2573 1258 1156 773 596 134
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2181 2368 2443
Size: 416 53 83 399 24 764 187 75
Cuts at: 2443 2506 2518 4221 5179 442
Size: 63 12 1703 958 1753
Fragments arranged by size:
1753 1703 958 764 416 399 187 83
75 63 53 24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 2421 3737 5150 1239
Size: 1117 65 1316 1413 2579
Fragments arranged by size:
2579 1413 1316 1117 65
Bpu10I CC'TnA_GC
Cuts at: 2268 3758 3770 2268
Size: 1490 12 4988
Fragments arranged by size:
4988 1490 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4615 5480 5778 4615
Size: 865 298 5327
Fragments arranged by size:
5327 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5153 882
Size: 617 3654 2219
Fragments arranged by size:
3654 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4281 493
Size: 711 3077 2702
Fragments arranged by size:
3077 2702 711
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4679 5314 275
Size: 53 83 186 936 2764 382 635 1451
Fragments arranged by size:
2764 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2101 3678 4923 5321 5595 5742 6153 2101
Size: 1577 1245 398 274 147 411 2438
Fragments arranged by size:
2438 1577 1245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2407 2437 1178
Size: 30 165 30 1004 30 5231
Fragments arranged by size:
5231 1004 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6490
BseYI C'CCAG_C
Cuts at: 2376 2385 5693 6189 2376
Size: 9 3308 496 2677
Fragments arranged by size:
3308 2677 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3268 1163
Size: 573 1532 4385
Fragments arranged by size:
4385 1532 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3940 4701 5729 1540
Size: 275 186 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3273 4551 4636 5707 3273
Size: 1278 85 1071 4056
Fragments arranged by size:
4056 1278 1071 85
BsmI GAATG_Cn'
Cuts at: 4001 4094 4001
Size: 93 6397
BsmBI CGTCTCn'nnnn_
Cuts at: 2897 2897
Size: 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2386 2407 2520 3273 3293 3809 4551
Size: 399 596 21 113 753 20 516 742
Cuts at: 4551 4636 5707 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 753 742 596 516 399 113
85 21 20
BspEI T'CCGG_A
Cuts at: 2101 6153 2101
Size: 4052 2438
BspHI T'CATG_A
Cuts at: 3135 4379 6109 3135
Size: 1244 1730 3516
Fragments arranged by size:
3516 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3845 844
Size: 3001 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4377 1394
Size: 429 2554 3507
Fragments arranged by size:
3507 2554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2323 4985 5159 65
Size: 1183 1075 2662 174 1396
Fragments arranged by size:
2662 1396 1183 1075 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5134 5321 1700
Size: 110 3324 187 2869
Fragments arranged by size:
3324 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 3434 3485 4550 5562 3434
Size: 51 1065 1012 4362
Fragments arranged by size:
4362 1065 1012 51
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3727 794
Size: 2933 3557
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstXI CCAn_nnnn'nTGG
Cuts at: 3229 3436 3229
Size: 207 6283
BstYI r'GATC_y
Cuts at: 1972 2770 3379 3457 3681 3820 4187 4572 4589
Size: 798 609 78 224 139 367 385 17
Cuts at: 4589 6041 6127 6139 6485 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 798 609 385 367 346 224
139 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3150 3228 513
Size: 552 939 1146 78 3775
Fragments arranged by size:
3775 1146 939 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5751
Fragments arranged by size:
5751 683 56
BtsI GCAGTG_nn'
Cuts at: 2317 2439 4006 4800 4820 2317
Size: 122 1567 794 20 3987
Fragments arranged by size:
3987 1567 794 122 20
ClaI AT'CG_AT
Cuts at: 1554 2845 1554
Size: 1291 5199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2945 5497 809
Size: 507 1629 2552 1802
Fragments arranged by size:
2552 1802 1629 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2449 2667
Size: 54 1425 50 275 18 177 442 218
Cuts at: 2667 3339 3937 4826 6455 8
Size: 672 598 889 1629 43
Fragments arranged by size:
1629 1425 889 672 598 442 275 218
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3937 1537
Size: 275 2125 4090
Fragments arranged by size:
4090 2125 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2533 2937 2958 3614 4420 1353
Size: 1180 404 21 656 806 3423
Fragments arranged by size:
3423 1180 806 656 404 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3695 5077 5451 5597 387
Size: 1064 138 2106 1382 374 146 1280
Fragments arranged by size:
2106 1382 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2356 2421 2981 3737 4553 5150 5937
Size: 393 724 65 560 756 816 597 787
Cuts at: 5937 6205 1239
Size: 268 1524
Fragments arranged by size:
1524 816 787 756 724 597 560 393
268 65
EcoO109I rG'GnC_Cy
Cuts at: 2382 3766 2382
Size: 1384 5106
EcoRI G'AATT_C
Cuts at: 3799 4177 3799
Size: 378 6112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2258 3518 2258
Size: 1260 5230
FspI TGC'GCA
Cuts at: 2984 4996 2984
Size: 2012 4478
FspAI rTGC'GCAy
Cuts at: 2984 2984
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 2571 3283 5637 1678
Size: 893 712 2354 2531
Fragments arranged by size:
2531 2354 893 712
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 2237 2269 2312
Size: 32 135 32 498 32 135 32 43
Cuts at: 2312 2344 2534 2566 3615 3647 5131 5163 5205
Size: 32 190 32 1049 32 1484 32 42
Cuts at: 5205 5237 1373
Size: 32 2626
Fragments arranged by size:
2626 1484 1049 498 190 135 135 43
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6490
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 3642 6366 3642
Size: 2724 3766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2692 5604 5788 1305
Size: 1387 2912 184 2007
Fragments arranged by size:
2912 2007 1387 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2669 3341 10
Size: 54 1425 343 177 660 672 3159
Fragments arranged by size:
3159 1425 672 660 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4448 4807 4966 518
Size: 648 3282 359 159 2042
Fragments arranged by size:
3282 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2555 3906 4583 5731 5976 1606
Size: 949 1351 677 1148 245 2120
Fragments arranged by size:
2120 1351 1148 949 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3150 3228 513
Size: 552 939 1146 78 3775
Fragments arranged by size:
3775 1146 939 552 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NspI r_CATG'y
Cuts at: 1132 1229 3848 5393 1132
Size: 97 2619 1545 2229
Fragments arranged by size:
2619 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 5389 5389
Size: 6490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2433 2480 1108
Size: 1325 47 5118
Fragments arranged by size:
5118 1325 47
PfoI T'CCnGG_A
Cuts at: 2629 2629
Size: 6490
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2142 2174 4559 4591 6072 6104 2142
Size: 32 2385 32 1481 32 2528
Fragments arranged by size:
2528 2385 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PspOMI G'GGCC_C
Cuts at: 2382 2516 3289 2382
Size: 134 773 5583
Fragments arranged by size:
5583 773 134
PstI C_TGCA'G
Cuts at: 830 3842 830
Size: 3012 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 2555 2555
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SexAI A'CCwGG_T
Cuts at: 2301 2301
Size: 6490
SfcI C'TryA_G
Cuts at: 826 1045 3838 4973 5654 5845 826
Size: 219 2793 1135 681 191 1471
Fragments arranged by size:
2793 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4594 5495 5757 820
Size: 197 926 2651 901 262 1553
Fragments arranged by size:
2651 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 3571 152
Size: 3419 3071
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 2462 3002 3326 1517
Size: 945 540 324 4681
Fragments arranged by size:
4681 945 540 324
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 2467 3150 3222 3228 3300
Size: 552 939 9 454 683 72 6 72
Cuts at: 3300 3929 513
Size: 629 3074
Fragments arranged by size:
3074 939 683 629 552 454 72 72
9 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4517 4534 4687 4872 5317 325
Size: 1255 2937 17 153 185 445 1498
Fragments arranged by size:
2937 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4736 6448 96
Size: 274 80 33 51 1443 2759 1712 138
Fragments arranged by size:
2759 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2208 2240 2407 2439 6450 6482 2208
Size: 32 167 32 4011 32 2216
Fragments arranged by size:
4011 2216 167 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2309
Size: 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 4619 4619
Size: 6490
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI