Restriction map for
pFN21AB6140
(Circular) (Six-base) MAPSORT of: pFN21AB6140.seq Check: 6026 from: 1 to: 6607
pFN21AB6140 6607 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3950 1937
Size: 2013 4594
Acc65I G'GTAC_C
Cuts at: 3928 3928
Size: 6607
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2100 2439 2468 2619 2966 3401 3521 4670
Size: 468 339 29 151 347 435 120 1149
Cuts at: 4670 6054 6322 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1149 468 435 347 339 268
151 120 29
AfeI AGC'GCT
Cuts at: 2046 2046
Size: 6607
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 3252 3636 5506 6483 1203
Size: 2049 384 1870 977 1327
Fragments arranged by size:
2049 1870 1327 977 384
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 2281 5336 2281
Size: 3055 3552
AleI CACnn'nnGTG
Cuts at: 1166 2993 1166
Size: 1827 4780
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6607
AlwNI CAG_nnn'CTG
Cuts at: 2549 4350 5922 2549
Size: 1801 1572 3234
Fragments arranged by size:
3234 1801 1572
ApaI G_GGCC'C
Cuts at: 2250 2250
Size: 6607
ApaLI G'TGCA_C
Cuts at: 3258 3815 4664 5820 3258
Size: 557 849 1156 4045
Fragments arranged by size:
4045 1156 849 557
ApoI r'AATT_y
Cuts at: 1493 3916 4142 4294 6403 1493
Size: 2423 226 152 2109 1697
Fragments arranged by size:
2423 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 2341 2347 2820 3086 3240 3600 3621
Size: 73 398 6 473 266 154 360 21
Cuts at: 3621 3932 1870
Size: 311 4545
Fragments arranged by size:
4545 473 398 360 311 266 154 73
21 6
BamHI G'GATC_C
Cuts at: 3937 4304 3937
Size: 367 6240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2830 3308 3928 5383 618
Size: 325 217 627 1043 478 620 1455 1842
Fragments arranged by size:
1842 1455 1043 627 620 478 325 217
BanII G_rGCy'C
Cuts at: 1391 2250 2348 2723 2758 3249 3477 3827 3926
Size: 859 98 375 35 491 228 350 99
Cuts at: 3926 1391
Size: 4072
Fragments arranged by size:
4072 859 491 375 350 228 99 98
35
BbeI G_GCGC'C
Cuts at: 3312 3312
Size: 6607
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3083 3767 928
Size: 971 1184 684 3768
Fragments arranged by size:
3768 1184 971 684
BbvCI CC'TCA_GC
Cuts at: 2186 3770 2186
Size: 1584 5023
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4798 4832 1903
Size: 34 2861 34 3678
Fragments arranged by size:
3678 2861 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4499 5709 4499
Size: 1210 5397
BclI T'GATC_A
Cuts at: 1548 2436 1548
Size: 888 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2822 3053 5218
Size: 107 122 71 631 605 1150 231 2165
Cuts at: 5218 136
Size: 1525
Fragments arranged by size:
2165 1525 1150 631 605 231 122 107
71
BglII A'GATC_T
Cuts at: 6602 6602
Size: 6607
BlpI GC'TnA_GC
Cuts at: 2757 4024 2757
Size: 1267 5340
Bme1580I G_kGCm'C
Cuts at: 1790 2250 2594 2835 3262 3449 3819 4668 5824
Size: 460 344 241 427 187 370 849 1156
Cuts at: 5824 1790
Size: 2573
Fragments arranged by size:
2573 1156 849 460 427 370 344 241
187
BmgBI CAC'GTC
Cuts at: 1318 2311 1318
Size: 993 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2829 3883 4338
Size: 416 53 83 399 24 1412 1054 455
Cuts at: 4338 5296 442
Size: 958 1753
Fragments arranged by size:
1753 1412 1054 958 455 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 3872 2356
Size: 1516 5091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 3009 3092 3194 3218 3571 3580 5267
Size: 1370 400 83 102 24 353 9 1687
Cuts at: 5267 1239
Size: 2579
Fragments arranged by size:
2579 1687 1370 400 353 102 83 24
9
Bpu10I CC'TnA_GC
Cuts at: 2154 2186 2970 3541 3770 2154
Size: 32 784 571 229 4991
Fragments arranged by size:
4991 784 571 229 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3341 4732 5597 5895 3341
Size: 1391 865 298 4053
Fragments arranged by size:
4053 1391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2287 5270 882
Size: 617 788 2983 2219
Fragments arranged by size:
2983 2219 788 617
BsaAI yAC'GTr
Cuts at: 493 1204 3282 3900 4398 493
Size: 711 2078 618 498 2702
Fragments arranged by size:
2702 2078 711 618 498
BsaBI GATnn'nnATC
Cuts at: 6601 6601
Size: 6607
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2693 2825 3309 3617
Size: 53 83 186 936 1160 132 484 308
Cuts at: 3617 3764 4414 4796 5431 275
Size: 147 650 382 635 1451
Fragments arranged by size:
1451 1160 936 650 635 484 382 308
186 147 132 83 53
BsaWI w'CCGG_w
Cuts at: 5040 5438 5712 5859 6270 5040
Size: 398 274 147 411 5377
Fragments arranged by size:
5377 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2557 2587 2790 2820 3809
Size: 30 165 30 1154 30 203 30 989
Cuts at: 3809 3839 1178
Size: 30 3946
Fragments arranged by size:
3946 1154 989 203 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2305 2798 3191 3311 3817 1186
Size: 1119 493 393 120 506 3976
Fragments arranged by size:
3976 1119 506 493 393 120
BseYI C'CCAG_C
Cuts at: 2704 2935 3109 3134 5810 6306 2704
Size: 231 174 25 2676 496 3005
Fragments arranged by size:
3005 2676 496 231 174 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2861 3206 3441 3617 1163
Size: 573 1125 345 235 176 4153
Fragments arranged by size:
4153 1125 573 345 235 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3045 3225 3834 4057 4818 5846
Size: 275 186 1044 180 609 223 761 1028
Cuts at: 5846 1540
Size: 2301
Fragments arranged by size:
2301 1044 1028 761 609 275 223 186
180
BsiHKAI G_wGCw'C
Cuts at: 2322 2561 2723 3262 3369 3819 3827 4668 4753
Size: 239 162 539 107 450 8 841 85
Cuts at: 4753 5824 2322
Size: 1071 3105
Fragments arranged by size:
3105 1071 841 539 450 239 162 107
85 8
BsmI GAATG_Cn'
Cuts at: 4118 4211 4118
Size: 93 6514
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2322 2348 2561 2594 2723 2758
Size: 399 460 72 26 213 33 129 35
Cuts at: 2758 2835 3249 3262 3369 3449 3477 3819 3827
Size: 77 414 13 107 80 28 342 8
Cuts at: 3827 3926 4668 4753 5824 1391
Size: 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 414 399 342 213
129 107 99 85 80 77 72 35
33 28 26 13 8
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 2403 4496 6226 2403
Size: 2093 1730 2784
Fragments arranged by size:
2784 2093 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2156 3496 3962 844
Size: 1312 1340 466 3489
Fragments arranged by size:
3489 1340 1312 466
BsrBI CCG'CTC
Cuts at: 1394 1823 2969 3306 3540 3879 4494 1394
Size: 429 1146 337 234 339 615 3507
Fragments arranged by size:
3507 1146 615 429 339 337 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 5102 5276 65
Size: 1183 3854 174 1396
Fragments arranged by size:
3854 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3375 3698 3728 5251 5438 1700
Size: 110 1565 323 30 1523 187 2869
Fragments arranged by size:
2869 1565 1523 323 187 110 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6607
BssSI C'ACGA_G
Cuts at: 2480 4667 5679 2480
Size: 2187 1012 3408
Fragments arranged by size:
3408 2187 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6607
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 3007 1184
Size: 1823 4784
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 2215 2995 3685 2209
Size: 6 780 690 5131
Fragments arranged by size:
5131 780 690 6
BstYI r'GATC_y
Cuts at: 1972 3090 3937 4304 4689 4706 6158 6244 6256
Size: 1118 847 367 385 17 1452 86 12
Cuts at: 6256 6602 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1118 847 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2202 2208 2214 2250 2367 3834 513
Size: 552 1137 6 6 36 117 1467 3286
Fragments arranged by size:
3286 1467 1137 552 117 36 6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2256 487
Size: 683 56 1030 4838
Fragments arranged by size:
4838 1030 683 56
BtsI GCAGTG_nn'
Cuts at: 2659 4123 4917 4937 2659
Size: 1464 794 20 4329
Fragments arranged by size:
4329 1464 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 2124 3911 2124
Size: 1787 4820
DraIII CAC_nnn'GTG
Cuts at: 2273 3496 2273
Size: 1223 5384
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5614 809
Size: 507 4298 1802
Fragments arranged by size:
4298 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2211 3042
Size: 54 1425 50 275 18 375 6 831
Cuts at: 3042 3222 3609 3675 3831 4054 4943 6572 8
Size: 180 387 66 156 223 889 1629 43
Fragments arranged by size:
1629 1425 889 831 387 375 275 223
180 156 66 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3042 3222 3831 4054 1537
Size: 275 1230 180 609 223 4090
Fragments arranged by size:
4090 1230 609 275 223 180
EarI CTCTTCn'nnn_
Cuts at: 1353 2638 3296 4537 1353
Size: 1285 658 1241 3423
Fragments arranged by size:
3423 1285 1241 658
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5194 5568 5714 387
Size: 1064 138 3605 374 146 1280
Fragments arranged by size:
3605 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2721 3825 2721
Size: 1104 5503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2439 2468 2609 2619 2966 3009
Size: 393 468 339 29 141 10 347 43
Cuts at: 3009 3092 3194 3218 3401 3521 3571 3580 4670
Size: 83 102 24 183 120 50 9 1090
Cuts at: 4670 5267 6054 6322 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1090 787 597 468 393 347 339
268 183 141 120 102 83 50 43
29 24 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3176 3440 3176
Size: 264 6343
EcoO109I rG'GnC_Cy
Cuts at: 2056 2398 2872 3783 2056
Size: 342 474 911 4880
Fragments arranged by size:
4880 911 474 342
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3406 3292
Size: 114 6493
FspI TGC'GCA
Cuts at: 2261 3062 5113 2261
Size: 801 2051 3755
Fragments arranged by size:
3755 2051 801
FspAI rTGC'GCAy
Cuts at: 2261 2261
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 2048 3059 3141 3312 3356 3404 3729 3744
Size: 370 1011 82 171 44 48 325 15
Cuts at: 3744 5754 1678
Size: 2010 2531
Fragments arranged by size:
2531 2010 1011 370 325 171 82 48
44 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 2557 2589 2844
Size: 32 135 32 695 32 258 32 255
Cuts at: 2844 2876 5248 5280 5322 5354 1373
Size: 32 2372 32 42 32 2626
Fragments arranged by size:
2626 2372 695 258 255 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2917 3951 4197 669
Size: 988 281 979 1034 246 3079
Fragments arranged by size:
3079 1034 988 979 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KasI G'GCGC_C
Cuts at: 3308 3308
Size: 6607
KpnI G_GTAC'C
Cuts at: 3932 3932
Size: 6607
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2195 2349 3774 5721 5905 1305
Size: 890 154 1425 1947 184 2007
Fragments arranged by size:
2007 1947 1425 890 184 154
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 10
Size: 54 1425 343 381 4404
Fragments arranged by size:
4404 1425 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2207 2213 2993 4565 4924 5083
Size: 648 978 63 6 780 1572 359 159
Cuts at: 5083 518
Size: 2042
Fragments arranged by size:
2042 1572 978 780 648 359 159 63
6
MspA1I CmG'CkG
Cuts at: 1606 2446 3070 3288 3430 3482 3595 4023 4700
Size: 840 624 218 142 52 113 428 677
Cuts at: 4700 5848 6093 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 840 677 624 428 245 218
142 113 52
NaeI GCC'GGC
Cuts at: 3377 3730 3377
Size: 353 6254
NarI GG'CG_CC
Cuts at: 3309 3309
Size: 6607
NcoI C'CATG_G
Cuts at: 513 1065 2208 2214 513
Size: 552 1143 6 4906
Fragments arranged by size:
4906 1143 552 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6607
NgoMIV G'CCGG_C
Cuts at: 3375 3728 3375
Size: 353 6254
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 3042 4054 3042
Size: 1012 5595
NspI r_CATG'y
Cuts at: 1132 1229 2022 2260 3256 3965 5510 1132
Size: 97 793 238 996 709 1545 2229
Fragments arranged by size:
2229 1545 996 793 709 238 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6607
PciI A'CATG_T
Cuts at: 3252 5506 3252
Size: 2254 4353
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6607
PfoI T'CCnGG_A
Cuts at: 3858 3858
Size: 6607
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PmlI CAC'GTG
Cuts at: 3282 3900 3282
Size: 618 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 4676 4708 6189 6221 3656
Size: 32 988 32 1481 32 4042
Fragments arranged by size:
4042 1481 988 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 3783 2056
Size: 1727 4880
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 4177 4177
Size: 6607
PspOMI G'GGCC_C
Cuts at: 2246 2246
Size: 6607
PspXI vC'TCGA_Gb
Cuts at: 2341 2341
Size: 6607
PstI C_TGCA'G
Cuts at: 830 3408 3441 3663 3959 830
Size: 2578 33 222 296 3478
Fragments arranged by size:
3478 2578 296 222 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 2446 3070 3595 2446
Size: 624 525 5458
Fragments arranged by size:
5458 624 525
SacI G_AGCT'C
Cuts at: 2723 3827 2723
Size: 1104 5503
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SanDI GG'GwC_CC
Cuts at: 2056 3783 2056
Size: 1727 4880
SapI GCTCTTCn'nnn_
Cuts at: 3296 3296
Size: 6607
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 4855 6567 4855
Size: 1712 4895
SexAI A'CCwGG_T
Cuts at: 3162 3162
Size: 6607
SfcI C'TryA_G
Cuts at: 826 1045 3404 3437 3578 3659 3955 5090 5771
Size: 219 2359 33 141 81 296 1135 681
Cuts at: 5771 5962 826
Size: 191 1471
Fragments arranged by size:
2359 1471 1135 681 296 219 191 141
81 33
SfoI GGC'GCC
Cuts at: 3310 3310
Size: 6607
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 2822 3242 3602 3934 1872
Size: 950 420 360 332 4545
Fragments arranged by size:
4545 950 420 360 332
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2341 3086 3356 4711 5612 5874
Size: 197 926 398 745 270 1355 901 262
Cuts at: 5874 820
Size: 1553
Fragments arranged by size:
1553 1355 926 901 745 398 270 262
197
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 2990 152
Size: 2838 3769
SphI G_CATG'C
Cuts at: 1132 2022 3965 1132
Size: 890 1943 3774
Fragments arranged by size:
3774 1943 890
SspI AAT'ATT
Cuts at: 5 52 4531 5
Size: 47 4479 2081
Fragments arranged by size:
4479 2081 47
StuI AGG'CCT
Cuts at: 1517 3584 1517
Size: 2067 4540
StyI C'CwwG_G
Cuts at: 513 1065 2176 2208 2214 3465 4046 513
Size: 552 1111 32 6 1251 581 3074
Fragments arranged by size:
3074 1251 1111 581 552 32 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 3115 3667 4634 4651 4804 4989
Size: 1255 444 1091 552 967 17 153 185
Cuts at: 4989 5434 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1091 967 552 445 444 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4853 6565 96
Size: 274 80 33 51 1443 2876 1712 138
Fragments arranged by size:
2876 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2790 2822 3266 3298 3628 3660 3809 3841 6567
Size: 32 444 32 330 32 149 32 2726
Cuts at: 6567 6599 2790
Size: 32 2798
Fragments arranged by size:
2798 2726 444 330 149 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6607
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XhoI C'TCGA_G
Cuts at: 1943 2341 3086 1943
Size: 398 745 5464
Fragments arranged by size:
5464 745 398
XmaI C'CCGG_G
Cuts at: 1870 2820 3240 3600 3932 1870
Size: 950 420 360 332 4545
Fragments arranged by size:
4545 950 420 360 332
XmnI GAAnn'nnTTC
Cuts at: 4736 4736
Size: 6607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI NruI NsiI PacI PsrI
RsrII SacII SfiI SrfI SwaI XcmI