Restriction map for
pFN21AB6136
(Circular) (Six-base) MAPSORT of: pFN21AB6136.seq Check: 6026 from: 1 to: 6622
pFN21AB6136 6622 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2671 3337 2671
Size: 666 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3965 1937
Size: 2028 4594
Acc65I G'GTAC_C
Cuts at: 2973 3131 3943 2973
Size: 158 812 5652
Fragments arranged by size:
5652 812 158
AclI AA'CG_TT
Cuts at: 1563 4749 5122 1563
Size: 3186 373 3063
Fragments arranged by size:
3186 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 2463 3026 3354 4685 6069 6337 1632
Size: 567 264 563 328 1331 1384 268 1917
Fragments arranged by size:
1917 1384 1331 567 563 328 268 264
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 5521 6498 1203
Size: 4318 977 1327
Fragments arranged by size:
4318 1327 977
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 2458 2950 5351 2458
Size: 492 2401 3729
Fragments arranged by size:
3729 2401 492
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6622
AlwNI CAG_nnn'CTG
Cuts at: 3127 3632 4365 5937 3127
Size: 505 733 1572 3812
Fragments arranged by size:
3812 1572 733 505
ApaI G_GGCC'C
Cuts at: 2362 3365 3649 3779 2362
Size: 1003 284 130 5205
Fragments arranged by size:
5205 1003 284 130
ApaLI G'TGCA_C
Cuts at: 4679 5835 4679
Size: 1156 5466
ApoI r'AATT_y
Cuts at: 1493 2198 2432 2654 3931 4157 4309 6418 1493
Size: 705 234 222 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 705 234 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 3650 3710 3780 3947 1870
Size: 73 1707 60 70 167 4545
Fragments arranged by size:
4545 1707 167 73 70 60
BamHI G'GATC_C
Cuts at: 3097 3952 4319 3097
Size: 855 367 5400
Fragments arranged by size:
5400 855 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2973 3036 3131 3590
Size: 325 217 627 255 931 63 95 459
Cuts at: 3590 3794 3943 5398 618
Size: 204 149 1455 1842
Fragments arranged by size:
1842 1455 931 627 459 325 255 217
204 149 95 63
BanII G_rGCy'C
Cuts at: 1391 2137 2323 2362 2719 3365 3426 3649 3657
Size: 746 186 39 357 646 61 223 8
Cuts at: 3657 3718 3779 3941 1391
Size: 61 61 162 4072
Fragments arranged by size:
4072 746 646 357 223 186 162 61
61 61 39 8
BbeI G_GCGC'C
Cuts at: 3040 3594 3040
Size: 554 6068
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5651
BbvCI CC'TCA_GC
Cuts at: 2484 2913 3012 3548 2484
Size: 429 99 536 5558
Fragments arranged by size:
5558 536 429 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3691 3725 4813 4847 1903
Size: 34 1754 34 1088 34 3678
Fragments arranged by size:
3678 1754 1088 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4514 5724 3386
Size: 1128 1210 4284
Fragments arranged by size:
4284 1210 1128
BclI T'GATC_A
Cuts at: 1548 3004 1548
Size: 1456 5166
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 2480 3599
Size: 107 122 71 631 605 475 333 1119
Cuts at: 3599 3866 5233 136
Size: 267 1367 1525
Fragments arranged by size:
1525 1367 1119 631 605 475 333 267
122 107 71
BglII A'GATC_T
Cuts at: 6617 6617
Size: 6622
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2239 2362 3365 3649 3779 4683 5839
Size: 283 166 123 1003 284 130 904 1156
Cuts at: 5839 1790
Size: 2573
Fragments arranged by size:
2573 1156 1003 904 284 283 166 130
123
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2365 3368 4353
Size: 416 53 83 399 24 948 1003 985
Cuts at: 4353 5311 442
Size: 958 1753
Fragments arranged by size:
1753 1003 985 958 948 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 6622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 2711 3212 3641 3764 3835 5282 1239
Size: 1161 311 501 429 123 71 1447 2579
Fragments arranged by size:
2579 1447 1161 501 429 311 123 71
Bpu10I CC'TnA_GC
Cuts at: 2484 2718 2913 3012 3102 3548 2484
Size: 234 195 99 90 446 5558
Fragments arranged by size:
5558 446 234 195 99 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4747 5612 5910 4747
Size: 865 298 5459
Fragments arranged by size:
5459 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5285 882
Size: 617 3786 2219
Fragments arranged by size:
3786 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4413 493
Size: 711 3209 2702
Fragments arranged by size:
3209 2702 711
BsaBI GATnn'nnATC
Cuts at: 2565 6616 2565
Size: 4051 2571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3037 3591 4429 4811
Size: 53 83 186 936 1504 554 838 382
Cuts at: 4811 5446 275
Size: 635 1451
Fragments arranged by size:
1504 1451 936 838 635 554 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2696 3672 5055 5453 5727 5874 6285 2696
Size: 976 1383 398 274 147 411 3033
Fragments arranged by size:
3033 1383 976 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2373 2403 2501 2531 3440
Size: 30 165 30 970 30 98 30 909
Cuts at: 3440 3470 1178
Size: 30 4330
Fragments arranged by size:
4330 970 909 165 98 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 2591 2906 2999 3002 1186
Size: 1336 69 315 93 3 4806
Fragments arranged by size:
4806 1336 315 93 69 3
BseYI C'CCAG_C
Cuts at: 2478 2521 3365 5825 6321 2478
Size: 43 844 2460 496 2779
Fragments arranged by size:
2779 2460 844 496 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2721 3110 3494 1163
Size: 573 985 389 384 4291
Fragments arranged by size:
4291 985 573 389 384
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2551 4072 4833 5861 1540
Size: 275 186 550 1521 761 1028 2301
Fragments arranged by size:
2301 1521 1028 761 550 275 186
BsiHKAI G_wGCw'C
Cuts at: 2719 3426 3657 3738 3821 4683 4768 5839 2719
Size: 707 231 81 83 862 85 1071 3502
Fragments arranged by size:
3502 1071 862 707 231 85 83 81
BsmI GAATG_Cn'
Cuts at: 2039 2221 4133 4226 2039
Size: 182 1912 93 4435
Fragments arranged by size:
4435 1912 182 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2137 2239 2323 2362 2719 3365
Size: 399 283 64 102 84 39 357 646
Cuts at: 3365 3426 3649 3657 3718 3738 3779 3821 3941
Size: 61 223 8 61 20 41 42 120
Cuts at: 3941 4683 4768 5839 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 646 399 357 283 223
120 102 85 84 64 61 61 42
41 39 20 8
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 2055 4511 6241 2055
Size: 2456 1730 2436
Fragments arranged by size:
2456 2436 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2671 3063 3337 3977 844
Size: 1827 392 274 640 3489
Fragments arranged by size:
3489 1827 640 392 274
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 4509 1394
Size: 429 230 2456 3507
Fragments arranged by size:
3507 2456 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 3176 5117 5291 65
Size: 1183 768 1160 1941 174 1396
Fragments arranged by size:
1941 1396 1183 1160 768 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5266 5453 1700
Size: 110 3456 187 2869
Fragments arranged by size:
3456 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6622
BssSI C'ACGA_G
Cuts at: 4682 5694 4682
Size: 1012 5610
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3632 3699 794
Size: 2838 67 3717
Fragments arranged by size:
3717 2838 67
BstBI TT'CG_AA
Cuts at: 5426 5426
Size: 6622
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6622
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2164
Size: 6622
BstYI r'GATC_y
Cuts at: 1972 2581 3097 3383 3883 3952 4319 4704 4721
Size: 609 516 286 500 69 367 385 17
Cuts at: 4721 6173 6259 6271 6617 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 609 516 500 385 367 346
286 86 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 2532 2574 2532
Size: 42 6580
BtgI C'CryG_G
Cuts at: 513 1065 2004 3525 513
Size: 552 939 1521 3610
Fragments arranged by size:
3610 1521 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3870 487
Size: 683 56 2644 3239
Fragments arranged by size:
3239 2644 683 56
BtsI GCAGTG_nn'
Cuts at: 3482 4138 4932 4952 3482
Size: 656 794 20 5152
Fragments arranged by size:
5152 794 656 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3487 3522 529
Size: 35 2923 35 3629
Fragments arranged by size:
3629 2923 35 35
DraI TTT'AAA
Cuts at: 3926 3926
Size: 6622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2422 5629 809
Size: 507 1106 3207 1802
Fragments arranged by size:
3207 1802 1106 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2595 2610 3600
Size: 54 1425 50 275 18 765 15 990
Cuts at: 3600 4069 4958 6587 8
Size: 469 889 1629 43
Fragments arranged by size:
1629 1425 990 889 765 469 275 54
50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4069 1537
Size: 275 2257 4090
Fragments arranged by size:
4090 2257 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3431 4552 1353
Size: 2078 1121 3423
Fragments arranged by size:
3423 2078 1121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2291 5209 5583 5729 387
Size: 1064 138 702 2918 374 146 1280
Fragments arranged by size:
2918 1280 1064 702 374 146 138
EcoICRI GAG'CTC
Cuts at: 2717 3424 3655 2717
Size: 707 231 5684
Fragments arranged by size:
5684 707 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2400 2463 2711 3026 3212 3354
Size: 393 567 201 63 248 315 186 142
Cuts at: 3354 3641 3764 3835 4685 5282 6069 6337 1239
Size: 287 123 71 850 597 787 268 1524
Fragments arranged by size:
1524 850 787 597 567 393 315 287
268 248 201 186 142 123 71 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 3044 3917 2096
Size: 948 873 4801
Fragments arranged by size:
4801 948 873
EcoO109I rG'GnC_Cy
Cuts at: 2089 3052 3362 3646 3748 3775 3776 2089
Size: 963 310 284 102 27 1 4935
Fragments arranged by size:
4935 963 310 284 102 27 1
EcoRI G'AATT_C
Cuts at: 2198 2432 3931 4309 2198
Size: 234 1499 378 4511
Fragments arranged by size:
4511 1499 378 234
FspI TGC'GCA
Cuts at: 5128 5128
Size: 6622
HaeII r_GCGC'y
Cuts at: 1678 3040 3230 3594 5769 1678
Size: 1362 190 364 2175 2531
Fragments arranged by size:
2531 2175 1362 364 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 2936 2968 3440
Size: 32 135 32 872 32 460 32 472
Cuts at: 3440 3472 5263 5295 5337 5369 1373
Size: 32 1791 32 42 32 2626
Fragments arranged by size:
2626 1791 872 472 460 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2779 3966 4212 669
Size: 988 281 841 1187 246 3079
Fragments arranged by size:
3079 1187 988 841 281 246
HindIII A'AGCT_T
Cuts at: 748 3114 748
Size: 2366 4256
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KasI G'GCGC_C
Cuts at: 3036 3590 3036
Size: 554 6068
KpnI G_GTAC'C
Cuts at: 2977 3135 3947 2977
Size: 158 812 5652
Fragments arranged by size:
5652 812 158
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 3783 5736 5920 1305
Size: 1563 915 1953 184 2007
Fragments arranged by size:
2007 1953 1563 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 3602 10
Size: 54 1425 343 780 990 3030
Fragments arranged by size:
3030 1425 990 780 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4580 4939 5098 518
Size: 648 843 2571 359 159 2042
Fragments arranged by size:
2571 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2378 2478 3086 3437 3542 3699 4038 4715
Size: 772 100 608 351 105 157 339 677
Cuts at: 4715 5863 6108 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 608 351 339 245
157 105 100
NarI GG'CG_CC
Cuts at: 3037 3591 3037
Size: 554 6068
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5131
Fragments arranged by size:
5131 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 3760 3980 5525 1132
Size: 97 2531 220 1545 2229
Fragments arranged by size:
2531 2229 1545 220 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6622
PciI A'CATG_T
Cuts at: 5521 5521
Size: 6622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6622
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3052 3052
Size: 6622
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6622
PsiI TTA'TAA
Cuts at: 2409 4192 2409
Size: 1783 4839
PspOMI G'GGCC_C
Cuts at: 2358 3361 3645 3775 2358
Size: 1003 284 130 5205
Fragments arranged by size:
5205 1003 284 130
PspXI vC'TCGA_Gb
Cuts at: 3650 3650
Size: 6622
PstI C_TGCA'G
Cuts at: 830 2208 2589 3261 3974 830
Size: 1378 381 672 713 3478
Fragments arranged by size:
3478 1378 713 672 381
PvuI CG_AT'CG
Cuts at: 2001 2551 2001
Size: 550 6072
PvuII CAG'CTG
Cuts at: 2378 3086 3437 3542 3699 2378
Size: 708 351 105 157 5301
Fragments arranged by size:
5301 708 351 157 105
SacI G_AGCT'C
Cuts at: 2719 3426 3657 2719
Size: 707 231 5684
Fragments arranged by size:
5684 707 231
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SapI GCTCTTCn'nnn_
Cuts at: 3431 3431
Size: 6622
SbfI CC_TGCA'GG
Cuts at: 2208 3974 2208
Size: 1766 4856
ScaI AGT'ACT
Cuts at: 4870 6582 4870
Size: 1712 4910
SfcI C'TryA_G
Cuts at: 826 1045 2204 2585 2844 3257 3970 5105 5786
Size: 219 1159 381 259 413 713 1135 681
Cuts at: 5786 5977 826
Size: 191 1471
Fragments arranged by size:
1471 1159 1135 713 681 413 381 259
219 191
SfoI GGC'GCC
Cuts at: 3038 3592 3038
Size: 554 6068
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 3782 3949 1872
Size: 1910 167 4545
Fragments arranged by size:
4545 1910 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3650 4726 5627 5889 820
Size: 197 926 1707 1076 901 262 1553
Fragments arranged by size:
1707 1553 1076 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3980 1132
Size: 2848 3774
SspI AAT'ATT
Cuts at: 5 52 2492 4546 5
Size: 47 2440 2054 2081
Fragments arranged by size:
2440 2081 2054 47
StuI AGG'CCT
Cuts at: 1517 2113 3269 1517
Size: 596 1156 4870
Fragments arranged by size:
4870 1156 596
StyI C'CwwG_G
Cuts at: 513 1065 2004 4061 513
Size: 552 939 2057 3074
Fragments arranged by size:
3074 2057 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 3152 4649 4666 4819 5004 5449
Size: 1255 513 1059 1497 17 153 185 445
Cuts at: 5449 325
Size: 1498
Fragments arranged by size:
1498 1497 1255 1059 513 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4868 6580 96
Size: 274 80 33 51 1443 2891 1712 138
Fragments arranged by size:
2891 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2373 2405 2501 2533 6582 6614 2373
Size: 32 96 32 4049 32 2381
Fragments arranged by size:
4049 2381 96 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6622
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2321 2605 2321
Size: 284 6338
XhoI C'TCGA_G
Cuts at: 1943 3650 1943
Size: 1707 4915
XmaI C'CCGG_G
Cuts at: 1870 3780 3947 1870
Size: 1910 167 4545
Fragments arranged by size:
4545 1910 167
XmnI GAAnn'nnTTC
Cuts at: 4751 4751
Size: 6622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI