Restriction map for
pFN21AB6135
(Circular) (Six-base) MAPSORT of: pFN21AB6135.seq Check: 4936 from: 1 to: 6481
pFN21AB6135 6481 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2589 3824 1937
Size: 652 1235 4594
Fragments arranged by size:
4594 1235 652
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 3147 4608 4981 1563
Size: 1584 1461 373 3063
Fragments arranged by size:
3063 1584 1461 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3155 4544 5928 6196 1632
Size: 1523 1389 1384 268 1917
Fragments arranged by size:
1917 1523 1389 1384 268
AfeI AGC'GCT
Cuts at: 2414 2516 2414
Size: 102 6379
AflII C'TTAA_G
Cuts at: 820 1017 2696 820
Size: 197 1679 4605
Fragments arranged by size:
4605 1679 197
AflIII A'CryG_T
Cuts at: 1203 2142 2910 3549 5380 6357 1203
Size: 939 768 639 1831 977 1327
Fragments arranged by size:
1831 1327 977 939 768 639
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 2973 3541 5210 2973
Size: 568 1669 4244
Fragments arranged by size:
4244 1669 568
AleI CACnn'nnGTG
Cuts at: 1166 2093 1166
Size: 927 5554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3005 3037 3275 3307 3005
Size: 32 238 32 6179
Fragments arranged by size:
6179 238 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4224 5796 4224
Size: 1572 4909
ApaI G_GGCC'C
Cuts at: 3215 3215
Size: 6481
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 2832 3790 4016 4168 6277 1493
Size: 1339 958 226 152 2109 1697
Fragments arranged by size:
2109 1697 1339 958 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 2293 3806 1870
Size: 73 350 1513 4545
Fragments arranged by size:
4545 1513 350 73
BamHI G'GATC_C
Cuts at: 3432 3811 4178 3432
Size: 379 367 5735
Fragments arranged by size:
5735 379 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3802 5257 618
Size: 325 217 627 2015 1455 1842
Fragments arranged by size:
2015 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 3215 3672 3800 1391
Size: 733 1091 457 128 4072
Fragments arranged by size:
4072 1091 733 457 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2340 928
Size: 971 441 5069
Fragments arranged by size:
5069 971 441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3680 3714 4672 4706 1903
Size: 34 50 34 1659 34 958 34 3678
Fragments arranged by size:
3678 1659 958 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4373 5583 4373
Size: 1210 5271
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6481
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5092 136
Size: 107 122 71 631 605 3420 1525
Fragments arranged by size:
3420 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3081 6476 3081
Size: 3395 3086
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 3171 3215 4542 5698 1790
Size: 1381 44 1327 1156 2573
Fragments arranged by size:
2573 1381 1327 1156 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2899 3580 4212
Size: 416 53 83 399 24 1482 681 632
Cuts at: 4212 5170 442
Size: 958 1753
Fragments arranged by size:
1753 1482 958 681 632 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2568 3437 3602 5141 1239
Size: 1329 869 165 1539 2579
Fragments arranged by size:
2579 1539 1329 869 165
Bpu10I CC'TnA_GC
Cuts at: 2304 2304
Size: 6481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2162 4606 5471 5769 2042
Size: 120 2444 865 298 2754
Fragments arranged by size:
2754 2444 865 298 120
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5144 882
Size: 617 3645 2219
Fragments arranged by size:
3645 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2911 4272 493
Size: 711 1707 1361 2702
Fragments arranged by size:
2702 1707 1361 711
BsaBI GATnn'nnATC
Cuts at: 3086 6475 3086
Size: 3389 3092
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 2607 4288 4670
Size: 53 83 186 936 567 507 1681 382
Cuts at: 4670 5305 275
Size: 635 1451
Fragments arranged by size:
1681 1451 936 635 567 507 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3429 4914 5312 5586 5733 6144 3429
Size: 1485 398 274 147 411 3766
Fragments arranged by size:
3766 1485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3275 3305 1178
Size: 30 165 30 1872 30 4354
Fragments arranged by size:
4354 1872 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2639 3068 3296 3785 1186
Size: 946 507 429 228 489 3882
Fragments arranged by size:
3882 946 507 489 429 228
BseYI C'CCAG_C
Cuts at: 2009 3313 5684 6180 2009
Size: 1304 2371 496 2310
Fragments arranged by size:
2371 2310 1304 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2281 2464 3089 3695 1163
Size: 573 402 143 183 625 606 3949
Fragments arranged by size:
3949 625 606 573 402 183 143
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 3078 3931 4692 5720 1540
Size: 275 186 46 1031 853 761 1028 2301
Fragments arranged by size:
2301 1031 1028 853 761 275 186 46
BsiHKAI G_wGCw'C
Cuts at: 2124 2300 3060 3233 4542 4627 5698 2124
Size: 176 760 173 1309 85 1071 2907
Fragments arranged by size:
2907 1309 1071 760 176 173 85
BsmI GAATG_Cn'
Cuts at: 3992 4085 3992
Size: 93 6388
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2300 3060 3171 3215 3233 3672
Size: 399 334 176 760 111 44 18 439
Cuts at: 3672 3800 4542 4627 5698 1391
Size: 128 742 85 1071 2174
Fragments arranged by size:
2174 1071 760 742 439 399 334 176
128 111 85 44 18
BspEI T'CCGG_A
Cuts at: 3429 6144 3429
Size: 2715 3766
BspHI T'CATG_A
Cuts at: 4370 6100 4370
Size: 1730 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2549 3836 844
Size: 1705 1287 3489
Fragments arranged by size:
3489 1705 1287
BsrBI CCG'CTC
Cuts at: 1394 1823 2040 2170 4368 1394
Size: 429 217 130 2198 3507
Fragments arranged by size:
3507 2198 429 217 130
BsrDI GCAATG_nn'
Cuts at: 65 1248 4976 5150 65
Size: 1183 3728 174 1396
Fragments arranged by size:
3728 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 3513 5125 5312 1700
Size: 110 208 1495 1612 187 2869
Fragments arranged by size:
2869 1612 1495 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 3107 4541 5553 3107
Size: 1434 1012 4035
Fragments arranged by size:
4035 1434 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2493 3237 794
Size: 1699 744 4038
Fragments arranged by size:
4038 1699 744
BstBI TT'CG_AA
Cuts at: 3729 5285 3729
Size: 1556 4925
BstEII G'GTnAC_C
Cuts at: 1184 2198 1184
Size: 1014 5467
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 2848
Size: 6481
BstYI r'GATC_y
Cuts at: 1972 3081 3432 3811 4178 4563 4580 6032 6118
Size: 1109 351 379 367 385 17 1452 86
Cuts at: 6118 6130 6476 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1109 385 379 367 351 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3051 3051
Size: 6481
BtgI C'CryG_G
Cuts at: 513 1065 2521 513
Size: 552 1456 4473
Fragments arranged by size:
4473 1456 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3073 3241 487
Size: 683 56 1847 168 3727
Fragments arranged by size:
3727 1847 683 168 56
BtsI GCAGTG_nn'
Cuts at: 3997 4791 4811 3997
Size: 794 20 5667
Fragments arranged by size:
5667 794 20
ClaI AT'CG_AT
Cuts at: 1554 2709 1554
Size: 1155 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 3471 3483 3785 3471
Size: 12 302 6167
Fragments arranged by size:
6167 302 12
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2392 3075
Size: 54 1425 50 275 18 214 348 683
Cuts at: 3075 3339 3515 3928 4817 6446 8
Size: 264 176 413 889 1629 43
Fragments arranged by size:
1629 1425 889 683 413 348 275 264
214 176 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2044 3075 3928 1537
Size: 275 232 1031 853 4090
Fragments arranged by size:
4090 1031 853 275 232
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 2405 4411 1353
Size: 876 176 2006 3423
Fragments arranged by size:
3423 2006 876 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 2234 5068 5442 5588 387
Size: 1064 138 453 192 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 453 374 192 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2122
Size: 6481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2568 3155 3437 3602 4544 5141 5928
Size: 393 936 587 282 165 942 597 787
Cuts at: 5928 6196 1239
Size: 268 1524
Fragments arranged by size:
1524 942 936 787 597 587 393 282
268 165
EcoO109I rG'GnC_Cy
Cuts at: 2461 2692 3013 3130 3212 3490 2461
Size: 231 321 117 82 278 5452
Fragments arranged by size:
5452 321 278 231 117 82
EcoRI G'AATT_C
Cuts at: 3790 4168 3790
Size: 378 6103
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 2416 2518 3314 3349 3599 5628 1678
Size: 738 102 796 35 250 2029 2531
Fragments arranged by size:
2531 2029 796 738 250 102 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2909 2941 2959 2991 3527
Size: 32 135 32 1337 32 18 32 536
Cuts at: 3527 3559 5122 5154 5196 5228 1373
Size: 32 1563 32 42 32 2626
Fragments arranged by size:
2626 1563 1337 536 135 42 32 32
32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3004 3825 4071 669
Size: 988 281 1066 821 246 3079
Fragments arranged by size:
3079 1066 988 821 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6481
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 3549 6357 3549
Size: 2808 3673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2926 5595 5779 1305
Size: 1621 2669 184 2007
Fragments arranged by size:
2669 2007 1621 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4659
Fragments arranged by size:
4659 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2846 4439 4798 4957 518
Size: 648 927 753 1593 359 159 2042
Fragments arranged by size:
2042 1593 927 753 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2536 3679 3897 4574 5722 5967 1606
Size: 930 1143 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 1143 930 677 245 218
NaeI GCC'GGC
Cuts at: 3515 3515
Size: 6481
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5929
NdeI CA'TA_TG
Cuts at: 387 2553 387
Size: 2166 4315
NgoMIV G'CCGG_C
Cuts at: 3513 3513
Size: 6481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 2044 3928 2044
Size: 1884 4597
NruI TCG'CGA
Cuts at: 2468 2468
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 2146 3031 3196 3839 5384 1132
Size: 97 917 885 165 643 1545 2229
Fragments arranged by size:
2229 1545 917 885 643 165 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 2142 5380 2142
Size: 3238 3243
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2367 3524 1108
Size: 1259 1157 4065
Fragments arranged by size:
4065 1259 1157
PfoI T'CCnGG_A
Cuts at: 2326 2657 3153 3414 2326
Size: 331 496 261 5393
Fragments arranged by size:
5393 496 331 261
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PmlI CAC'GTG
Cuts at: 2911 2911
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 4550 4582 6063 6095 3275
Size: 32 1243 32 1481 32 3661
Fragments arranged by size:
3661 1481 1243 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2692 3013 3130 3490 2692
Size: 321 117 360 5683
Fragments arranged by size:
5683 360 321 117
PshAI GACnn'nnGTC
Cuts at: 1613 2145 3633 1613
Size: 532 1488 4461
Fragments arranged by size:
4461 1488 532
PsiI TTA'TAA
Cuts at: 4051 4051
Size: 6481
PspOMI G'GGCC_C
Cuts at: 3211 3211
Size: 6481
PstI C_TGCA'G
Cuts at: 830 2546 2754 3833 830
Size: 1716 208 1079 3478
Fragments arranged by size:
3478 1716 1079 208
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
PvuII CAG'CTG
Cuts at: 3679 3679
Size: 6481
SacI G_AGCT'C
Cuts at: 2124 2124
Size: 6481
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SbfI CC_TGCA'GG
Cuts at: 2546 3833 2546
Size: 1287 5194
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SfcI C'TryA_G
Cuts at: 826 1045 2479 2542 2750 3124 3829 4964 5645
Size: 219 1434 63 208 374 705 1135 681
Cuts at: 5645 5836 826
Size: 191 1471
Fragments arranged by size:
1471 1434 1135 705 681 374 219 208
191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 2177 2696 4585 5486 5748
Size: 197 926 114 120 519 1889 901 262
Cuts at: 5748 820
Size: 1553
Fragments arranged by size:
1889 1553 926 901 519 262 197 120
114
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SspI AAT'ATT
Cuts at: 5 52 4405 5
Size: 47 4353 2081
Fragments arranged by size:
4353 2081 47
StuI AGG'CCT
Cuts at: 1517 2274 3207 1517
Size: 757 933 4791
Fragments arranged by size:
4791 933 757
StyI C'CwwG_G
Cuts at: 513 1065 2796 3920 513
Size: 552 1731 1124 3074
Fragments arranged by size:
3074 1731 1124 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 4508 4525 4678 4863 5308 325
Size: 1255 593 2335 17 153 185 445 1498
Fragments arranged by size:
2335 1498 1255 593 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4727 6439 96
Size: 274 80 33 51 1443 2750 1712 138
Fragments arranged by size:
2750 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6441 6473 6441
Size: 32 6449
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6481
XbaI T'CTAG_A
Cuts at: 3817 3817
Size: 6481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 6481
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 4610 4610
Size: 6481
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NarI NsiI PacI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI