Restriction map for
pFN21AB6134
(Circular) (Six-base) MAPSORT of: pFN21AB6134.seq Check: 8450 from: 1 to: 6598
pFN21AB6134 6598 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2963 4408 278
Size: 53 83 186 936 1427 1445 2468
Fragments arranged by size:
2468 1445 1427 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 3941 1937
Size: 694 1310 4594
Fragments arranged by size:
4594 1310 694
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 2999 3272 3434 3452 4661 6045 6313
Size: 1046 321 273 162 18 1209 1384 268
Cuts at: 6313 1632
Size: 1917
Fragments arranged by size:
1917 1384 1209 1046 321 273 268 162
18
AfeI AGC'GCT
Cuts at: 2837 3449 2837
Size: 612 5986
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 5497 6474 1203
Size: 4294 977 1327
Fragments arranged by size:
4294 1327 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 5327 5327
Size: 6598
AleI CACnn'nnGTG
Cuts at: 1166 3620 1166
Size: 2454 4144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2039 2818 1184
Size: 855 779 4964
Fragments arranged by size:
4964 855 779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 2700
Size: 32 6566
AlwNI CAG_nnn'CTG
Cuts at: 2547 2557 3061 3094 3538 4341 5913 2547
Size: 10 504 33 444 803 1572 3232
Fragments arranged by size:
3232 1572 803 504 444 33 10
ApaLI G'TGCA_C
Cuts at: 4655 5811 4655
Size: 1156 5442
ApoI r'AATT_y
Cuts at: 1493 3907 4133 4285 6394 1493
Size: 2414 226 152 2109 1697
Fragments arranged by size:
2414 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 3222 3923 1870
Size: 73 1248 31 701 4545
Fragments arranged by size:
4545 1248 701 73 31
BamHI G'GATC_C
Cuts at: 3770 3880 3928 4295 3770
Size: 110 48 367 6073
Fragments arranged by size:
6073 367 110 48
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2370 2685 3018 3156 3919
Size: 325 217 627 583 315 333 138 763
Cuts at: 3919 5374 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 763 627 583 333 325 315
217 138
BanII G_rGCy'C
Cuts at: 1391 2541 2565 2643 3917 1391
Size: 1150 24 78 1274 4072
Fragments arranged by size:
4072 1274 1150 78 24
BbeI G_GCGC'C
Cuts at: 3022 3160 3022
Size: 138 6460
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 2963 928
Size: 971 962 102 4563
Fragments arranged by size:
4563 971 962 102
BbvCI CC'TCA_GC
Cuts at: 2609 2609
Size: 6598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2923 2957 4789 4823 1903
Size: 34 986 34 1832 34 3678
Fragments arranged by size:
3678 1832 986 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2198 4490 5700 2198
Size: 2292 1210 3096
Fragments arranged by size:
3096 2292 1210
BclI T'GATC_A
Cuts at: 1548 2409 1548
Size: 861 5737
BfrBI ATG'CAT
Cuts at: 3658 3658
Size: 6598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2510 2519 2589
Size: 107 122 71 631 605 838 9 70
Cuts at: 2589 5209 136
Size: 2620 1525
Fragments arranged by size:
2620 1525 838 631 605 122 107 71
70 9
BglII A'GATC_T
Cuts at: 6593 6593
Size: 6598
BlpI GC'TnA_GC
Cuts at: 2540 4015 2540
Size: 1475 5123
Bme1580I G_kGCm'C
Cuts at: 1790 2688 4659 5815 1790
Size: 898 1971 1156 2573
Fragments arranged by size:
2573 1971 1156 898
BmgBI CAC'GTC
Cuts at: 1318 2062 1318
Size: 744 5854
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 4329 5287
Size: 416 53 83 399 24 1293 1619 958
Cuts at: 5287 442
Size: 1753
Fragments arranged by size:
1753 1619 1293 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2510 2579 3116 3317 3560 3693 5258
Size: 938 333 69 537 201 243 133 1565
Cuts at: 5258 1239
Size: 2579
Fragments arranged by size:
2579 1565 938 537 333 243 201 133
69
Bpu10I CC'TnA_GC
Cuts at: 2609 2609
Size: 6598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2081 4723 5588 5886 2012
Size: 69 2642 865 298 2724
Fragments arranged by size:
2724 2642 865 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5261 882
Size: 617 3762 2219
Fragments arranged by size:
3762 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2871 4389 493
Size: 711 1667 1518 2702
Fragments arranged by size:
2702 1667 1518 711
BsaBI GATnn'nnATC
Cuts at: 2933 6592 2933
Size: 3659 2939
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2960 3019 3157 4405
Size: 53 83 186 936 1427 59 138 1248
Cuts at: 4405 4787 5422 275
Size: 382 635 1451
Fragments arranged by size:
1451 1427 1248 936 635 382 186 138
83 59 53
BsaWI w'CCGG_w
Cuts at: 3685 5031 5429 5703 5850 6261 3685
Size: 1346 398 274 147 411 4022
Fragments arranged by size:
4022 1346 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3725 3755 1178
Size: 30 165 30 2322 30 4021
Fragments arranged by size:
4021 2322 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2507 3053 3083 3158 3161 3272 3275
Size: 1075 246 546 30 75 3 111 3
Cuts at: 3275 3314 3413 3452 3557 3733 1186
Size: 39 99 39 105 176 4051
Fragments arranged by size:
4051 1075 546 246 176 111 105 99
75 39 39 30 3 3
BseYI C'CCAG_C
Cuts at: 2091 2729 3528 3765 5801 6297 2091
Size: 638 799 237 2036 496 2392
Fragments arranged by size:
2392 2036 799 638 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 1163
Size: 573 378 5647
Fragments arranged by size:
5647 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4048 4809 5837 1540
Size: 275 186 2047 761 1028 2301
Fragments arranged by size:
2301 2047 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2271 2565 2643 4659 4744 5815 2271
Size: 294 78 2016 85 1071 3054
Fragments arranged by size:
3054 2016 1071 294 85 78
BsmI GAATG_Cn'
Cuts at: 4109 4202 4109
Size: 93 6505
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2271 2541 2565 2643 2688 3917 4659
Size: 399 481 270 24 78 45 1229 742
Cuts at: 4659 4744 5815 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1229 1071 742 481 399 270 85
78 45 24
BspEI T'CCGG_A
Cuts at: 6261 6261
Size: 6598
BspHI T'CATG_A
Cuts at: 4487 6217 4487
Size: 1730 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2445 3953 844
Size: 1601 1508 3489
Fragments arranged by size:
3489 1601 1508
BsrBI CCG'CTC
Cuts at: 1394 1823 3016 3079 3130 3513 4485 1394
Size: 429 1193 63 51 383 972 3507
Fragments arranged by size:
3507 1193 972 429 383 63 51
BsrDI GCAATG_nn'
Cuts at: 65 1248 3811 5093 5267 65
Size: 1183 2563 1282 174 1396
Fragments arranged by size:
2563 1396 1282 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3558 3648 5242 5429 1700
Size: 110 1748 90 1594 187 2869
Fragments arranged by size:
2869 1748 1594 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 2118 96
Size: 2022 4576
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 2624 4658 5670 2624
Size: 2034 1012 3552
Fragments arranged by size:
3552 2034 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3061 3316 3424 794
Size: 2267 255 108 3968
Fragments arranged by size:
3968 2267 255 108
BstBI TT'CG_AA
Cuts at: 5402 5402
Size: 6598
BstEII G'GTnAC_C
Cuts at: 1184 3688 1184
Size: 2504 4094
BstXI CCAn_nnnn'nTGG
Cuts at: 2929 3391 2929
Size: 462 6136
BstYI r'GATC_y
Cuts at: 1972 3770 3880 3928 4295 4680 4697 6149 6235
Size: 1798 110 48 367 385 17 1452 86
Cuts at: 6235 6247 6593 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1798 1452 385 367 346 110 86
48 17 12
Bsu36I CC'TnA_GG
Cuts at: 3864 3864
Size: 6598
BtgI C'CryG_G
Cuts at: 513 1065 2004 2148 513
Size: 552 939 144 4963
Fragments arranged by size:
4963 939 552 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5859
Fragments arranged by size:
5859 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 4114 4908 4928 2797
Size: 1317 794 20 4467
Fragments arranged by size:
4467 1317 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 2616 3027
Size: 54 1425 50 275 18 468 318 411
Cuts at: 3027 4045 4934 6563 8
Size: 1018 889 1629 43
Fragments arranged by size:
1629 1425 1018 889 468 411 318 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4045 1537
Size: 275 2233 4090
Fragments arranged by size:
4090 2233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2381 2570 2751 3030 3390 3399 3474
Size: 710 318 189 181 279 360 9 75
Cuts at: 3474 4528 1353
Size: 1054 3423
Fragments arranged by size:
3423 1054 710 360 318 279 189 181
75 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2351 3227 5185 5559 5705 387
Size: 1064 138 762 876 1958 374 146 1280
Fragments arranged by size:
1958 1280 1064 876 762 374 146 138
EcoICRI GAG'CTC
Cuts at: 2563 2641 2563
Size: 78 6520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2510 2579 2678 2999 3116 3272
Size: 393 545 333 69 99 321 117 156
Cuts at: 3272 3317 3434 3452 3560 3693 4661 5258 6045
Size: 45 117 18 108 133 968 597 787
Cuts at: 6045 6313 1239
Size: 268 1524
Fragments arranged by size:
1524 968 787 597 545 393 333 321
268 156 133 117 117 108 99 69
45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2237 2390 2660 2969 3521 2015
Size: 222 153 270 309 552 5092
Fragments arranged by size:
5092 552 309 270 222 153
EcoO109I rG'GnC_Cy
Cuts at: 2974 3325 3786 2974
Size: 351 461 5786
Fragments arranged by size:
5786 461 351
EcoRI G'AATT_C
Cuts at: 3907 4285 3907
Size: 378 6220
FspI TGC'GCA
Cuts at: 2015 5104 2015
Size: 3089 3509
HaeII r_GCGC'y
Cuts at: 1678 2839 2886 2905 3022 3160 3191 3451 3757
Size: 1161 47 19 117 138 31 260 306
Cuts at: 3757 5745 1678
Size: 1988 2531
Fragments arranged by size:
2531 1988 1161 306 260 138 117 47
31 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3347 3379 5239 5271 5313
Size: 32 135 32 1775 32 1860 32 42
Cuts at: 5313 5345 1373
Size: 32 2626
Fragments arranged by size:
2626 1860 1775 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3942 4188 669
Size: 988 281 2004 246 3079
Fragments arranged by size:
3079 2004 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6598
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KasI G'GCGC_C
Cuts at: 3018 3156 3018
Size: 138 6460
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 3663 4197 3663
Size: 534 6064
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5712 5896 1305
Size: 4407 184 2007
Fragments arranged by size:
4407 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 10
Size: 54 1425 343 786 3990
Fragments arranged by size:
3990 1425 786 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 2927 3620 3633 4556 4915 5074
Size: 648 1108 653 693 13 923 359 159
Cuts at: 5074 518
Size: 2042
Fragments arranged by size:
2042 1108 923 693 653 648 359 159
13
MspA1I CmG'CkG
Cuts at: 1606 2580 2722 2733 2788 2918 3001 3010 3061
Size: 974 142 11 55 130 83 9 51
Cuts at: 3061 3118 3277 3313 3430 3538 3765 4014 4691
Size: 57 159 36 117 108 227 249 677
Cuts at: 4691 5839 6084 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 974 677 249 245 227 159
142 130 117 108 83 57 55 51
36 11 9
NarI GG'CG_CC
Cuts at: 3019 3157 3019
Size: 138 6460
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5107
Fragments arranged by size:
5107 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6598
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NsiI A_TGCA'T
Cuts at: 3660 3660
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 2212 2845 3956 5501 1132
Size: 97 983 633 1111 1545 2229
Fragments arranged by size:
2229 1545 1111 983 633 97
PasI CC'CwG_GG
Cuts at: 1348 3775 1348
Size: 2427 4171
PciI A'CATG_T
Cuts at: 5497 5497
Size: 6598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6598
PfoI T'CCnGG_A
Cuts at: 2232 2556 2232
Size: 324 6274
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2974 3786 2974
Size: 812 5786
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6598
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspXI vC'TCGA_Gb
Cuts at: 3191 3191
Size: 6598
PstI C_TGCA'G
Cuts at: 830 2727 2946 3057 3066 3090 3108 3141 3183
Size: 1897 219 111 9 24 18 33 42
Cuts at: 3183 3489 3522 3950 830
Size: 306 33 428 3478
Fragments arranged by size:
3478 1897 428 306 219 111 42 33
33 24 18 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
PvuII CAG'CTG
Cuts at: 2722 2733 2918 3061 3430 3538 2722
Size: 11 185 143 369 108 5782
Fragments arranged by size:
5782 369 185 143 108 11
SacI G_AGCT'C
Cuts at: 2565 2643 2565
Size: 78 6520
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SapI GCTCTTCn'nnn_
Cuts at: 2570 3474 2570
Size: 904 5694
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SfcI C'TryA_G
Cuts at: 826 1045 2723 2942 3053 3062 3086 3104 3137
Size: 219 1678 219 111 9 24 18 33
Cuts at: 3137 3179 3485 3518 3946 5081 5762 5953 826
Size: 42 306 33 428 1135 681 191 1471
Fragments arranged by size:
1678 1471 1135 681 428 306 219 219
191 111 42 33 33 24 18 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2510 2519 2510
Size: 9 6589
SfoI GGC'GCC
Cuts at: 3020 3158 3020
Size: 138 6460
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 3224 3925 1872
Size: 1352 701 4545
Fragments arranged by size:
4545 1352 701
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 2096 3191 4702 5603 5865
Size: 197 926 84 69 1095 1511 901 262
Cuts at: 5865 820
Size: 1553
Fragments arranged by size:
1553 1511 1095 926 901 262 197 84
69
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6598
SphI G_CATG'C
Cuts at: 1132 3956 1132
Size: 2824 3774
SrfI GCCC'GGGC
Cuts at: 3224 3224
Size: 6598
SspI AAT'ATT
Cuts at: 5 52 4522 5
Size: 47 4470 2081
Fragments arranged by size:
4470 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6598
StyI C'CwwG_G
Cuts at: 513 1065 2004 3030 4037 513
Size: 552 939 1026 1007 3074
Fragments arranged by size:
3074 1026 1007 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2471 4625 4642 4795 4980 5425 325
Size: 1255 891 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 891 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2226 4844
Size: 274 80 33 51 1443 141 108 2618
Cuts at: 4844 6556 96
Size: 1712 138
Fragments arranged by size:
2618 1712 1443 274 141 138 108 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3725 3757 6558 6590 3725
Size: 32 2801 32 3733
Fragments arranged by size:
3733 2801 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6598
XbaI T'CTAG_A
Cuts at: 3934 3934
Size: 6598
XhoI C'TCGA_G
Cuts at: 1943 3191 1943
Size: 1248 5350
XmaI C'CCGG_G
Cuts at: 1870 3222 3923 1870
Size: 1352 701 4545
Fragments arranged by size:
4545 1352 701
XmnI GAAnn'nnTTC
Cuts at: 4727 4727
Size: 6598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2961 4406 276
Size: 53 83 186 936 1427 1445 2468
Fragments arranged by size:
2468 1445 1427 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BplI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI
SexAI SwaI XcmI