Restriction map for
pFN21AB6132
(Circular) (Six-base) MAPSORT of: pFN21AB6132.seq Check: 8170 from: 1 to: 6589
pFN21AB6132 6589 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2525 2525
Size: 6589
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2906 3932 1937
Size: 969 1026 4594
Fragments arranged by size:
4594 1026 969
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2906 4652 6036 6304 1632
Size: 1274 1746 1384 268 1917
Fragments arranged by size:
1917 1746 1384 1274 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 2373 2430 5488 6465 1203
Size: 1170 57 3058 977 1327
Fragments arranged by size:
3058 1327 1170 977 57
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2103 1184
Size: 919 5670
AlwNI CAG_nnn'CTG
Cuts at: 2405 2578 4332 5904 2405
Size: 173 1754 1572 3090
Fragments arranged by size:
3090 1754 1572 173
ApaI G_GGCC'C
Cuts at: 2044 3734 2044
Size: 1690 4899
ApaLI G'TGCA_C
Cuts at: 3161 4646 5802 3161
Size: 1485 1156 3948
Fragments arranged by size:
3948 1485 1156
ApoI r'AATT_y
Cuts at: 1493 3898 4124 4276 6385 1493
Size: 2405 226 152 2109 1697
Fragments arranged by size:
2405 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 2218 2289 2641 2735 2957 2988 3796
Size: 73 275 71 352 94 222 31 808
Cuts at: 3796 3914 1870
Size: 118 4545
Fragments arranged by size:
4545 808 352 275 222 118 94 73
71 31
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 2584 3263 3617 3839
Size: 325 217 627 541 256 679 354 222
Cuts at: 3839 3910 5365 618
Size: 71 1455 1842
Fragments arranged by size:
1842 1455 679 627 541 354 325 256
222 217 71
BanII G_rGCy'C
Cuts at: 1391 2013 2044 2227 2261 2268 3102 3375 3629
Size: 622 31 183 34 7 834 273 254
Cuts at: 3629 3734 3766 3908 1391
Size: 105 32 142 4072
Fragments arranged by size:
4072 834 622 273 254 183 142 105
34 32 31 7
BbeI G_GCGC'C
Cuts at: 2332 2332
Size: 6589
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3598 3697 928
Size: 971 1699 99 3820
Fragments arranged by size:
3820 1699 971 99
BbvCI CC'TCA_GC
Cuts at: 2343 2409 3305 2343
Size: 66 896 5627
Fragments arranged by size:
5627 896 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3127 3161 4780 4814 1903
Size: 34 1190 34 1619 34 3678
Fragments arranged by size:
3678 1619 1190 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4481 5691 4481
Size: 1210 5379
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2748 3558 3659
Size: 107 122 71 631 605 1076 810 101
Cuts at: 3659 5200 136
Size: 1541 1525
Fragments arranged by size:
1541 1525 1076 810 631 605 122 107
101 71
BglII A'GATC_T
Cuts at: 6584 6584
Size: 6589
BlpI GC'TnA_GC
Cuts at: 3101 3788 4006 3101
Size: 687 218 5684
Fragments arranged by size:
5684 687 218
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2589 3165 3734 4650 5806 1790
Size: 254 545 576 569 916 1156 2573
Fragments arranged by size:
2573 1156 916 576 569 545 254
BmgBI CAC'GTC
Cuts at: 1318 3046 1318
Size: 1728 4861
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4320 5278 442
Size: 416 53 83 399 24 2903 958 1753
Fragments arranged by size:
2903 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3537 3537
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2600 2996 3086 3116 3412 3478 3666
Size: 1013 348 396 90 30 296 66 188
Cuts at: 3666 5249 1239
Size: 1583 2579
Fragments arranged by size:
2579 1583 1013 396 348 296 188 90
66 30
Bpu10I CC'TnA_GC
Cuts at: 2343 2409 3305 3548 2343
Size: 66 896 243 5384
Fragments arranged by size:
5384 896 243 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4714 5579 5877 4714
Size: 865 298 5426
Fragments arranged by size:
5426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2464 5252 882
Size: 617 965 2788 2219
Fragments arranged by size:
2788 2219 965 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 2556 2803 3271 4380 493
Size: 711 1170 182 247 468 1109 2702
Fragments arranged by size:
2702 1170 1109 711 468 247 182
BsaBI GATnn'nnATC
Cuts at: 6583 6583
Size: 6589
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2329 2700 4396 4778
Size: 53 83 186 936 796 371 1696 382
Cuts at: 4778 5413 275
Size: 635 1451
Fragments arranged by size:
1696 1451 936 796 635 382 371 186
83 53
BsaWI w'CCGG_w
Cuts at: 3845 5022 5420 5694 5841 6252 3845
Size: 1177 398 274 147 411 4182
Fragments arranged by size:
4182 1177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3393 3423 3639 3669 1178
Size: 30 165 30 1990 30 216 30 4098
Fragments arranged by size:
4098 1990 216 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3680 3864 1186
Size: 2494 184 3911
Fragments arranged by size:
3911 2494 184
BseYI C'CCAG_C
Cuts at: 2833 2841 2894 5792 6288 2833
Size: 8 53 2898 496 3134
Fragments arranged by size:
3134 2898 496 53 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2146 2768 2825 3068 3162 1163
Size: 573 410 622 57 243 94 4590
Fragments arranged by size:
4590 622 573 410 243 94 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2215 2752 4039 4800 5828 1540
Size: 275 186 214 537 1287 761 1028 2301
Fragments arranged by size:
2301 1287 1028 761 537 275 214 186
BsiHKAI G_wGCw'C
Cuts at: 2227 2268 2802 3102 3165 4650 4735 5806 2227
Size: 41 534 300 63 1485 85 1071 3010
Fragments arranged by size:
3010 1485 1071 534 300 85 63 41
BsmI GAATG_Cn'
Cuts at: 4100 4193 4100
Size: 93 6496
BsmBI CGTCTCn'nnnn_
Cuts at: 2691 3396 3712 3873 2691
Size: 705 316 161 5407
Fragments arranged by size:
5407 705 316 161
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2044 2227 2261 2268 2589 2802
Size: 399 223 31 183 34 7 321 213
Cuts at: 2802 3102 3165 3375 3629 3734 3766 3908 4650
Size: 300 63 210 254 105 32 142 742
Cuts at: 4650 4735 5806 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 321 300 254 223
213 210 183 142 105 85 63 34
32 31 7
BspEI T'CCGG_A
Cuts at: 3845 6252 3845
Size: 2407 4182
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2525 2682 3944 844
Size: 1681 157 1262 3489
Fragments arranged by size:
3489 1681 1262 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2217 3000 4476 1394
Size: 429 394 783 1476 3507
Fragments arranged by size:
3507 1476 783 429 394
BsrDI GCAATG_nn'
Cuts at: 65 1248 5084 5258 65
Size: 1183 3836 174 1396
Fragments arranged by size:
3836 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 3821 5233 5420 1700
Size: 110 213 1798 1412 187 2869
Fragments arranged by size:
2869 1798 1412 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6589
BssHII G'CGCG_C
Cuts at: 1925 2033 1925
Size: 108 6481
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2405 3169 794
Size: 1611 764 4214
Fragments arranged by size:
4214 1611 764
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstXI CCAn_nnnn'nTGG
Cuts at: 2841 3064 3211 2841
Size: 223 147 6219
Fragments arranged by size:
6219 223 147
BstYI r'GATC_y
Cuts at: 1972 3919 4286 4671 4688 6140 6226 6238 6584
Size: 1947 367 385 17 1452 86 12 346
Cuts at: 6584 1972
Size: 1977
Fragments arranged by size:
1977 1947 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2907 2907
Size: 6589
BtgI C'CryG_G
Cuts at: 513 1065 2004 2045 2746 2793 3430 3586 3663
Size: 552 939 41 701 47 637 156 77
Cuts at: 3663 513
Size: 3439
Fragments arranged by size:
3439 939 701 637 552 156 77 47
41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3231 3570 487
Size: 683 56 2005 339 3506
Fragments arranged by size:
3506 2005 683 339 56
BtsI GCAGTG_nn'
Cuts at: 2403 2415 2710 3145 4105 4899 4919 2403
Size: 12 295 435 960 794 20 4073
Fragments arranged by size:
4073 960 794 435 295 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3893 3893
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5596 809
Size: 507 4280 1802
Fragments arranged by size:
4280 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2749 3433 3651
Size: 54 1425 50 275 18 919 684 218
Cuts at: 3651 3660 3666 4036 4925 6554 8
Size: 9 6 370 889 1629 43
Fragments arranged by size:
1629 1425 919 889 684 370 275 218
54 50 43 18 9 6
EagI C'GGCC_G
Cuts at: 1537 1812 2749 4036 1537
Size: 275 937 1287 4090
Fragments arranged by size:
4090 1287 937 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4519 1353
Size: 3166 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 2906 3756 5176 5550 5696
Size: 1064 138 522 795 850 1420 374 146
Cuts at: 5696 387
Size: 1280
Fragments arranged by size:
1420 1280 1064 850 795 522 374 146
138
EcoICRI GAG'CTC
Cuts at: 2225 2266 3100 2225
Size: 41 834 5714
Fragments arranged by size:
5714 834 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2600 2906 2996 3086 3116 3412
Size: 393 620 348 306 90 90 30 296
Cuts at: 3412 3478 3666 4652 5249 6036 6304 1239
Size: 66 188 986 597 787 268 1524
Fragments arranged by size:
1524 986 787 620 597 393 348 306
296 268 188 90 90 66 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2735 2735
Size: 6589
EcoO109I rG'GnC_Cy
Cuts at: 2040 2041 3003 3730 3731 2040
Size: 1 962 727 1 4898
Fragments arranged by size:
4898 962 727 1 1
EcoRI G'AATT_C
Cuts at: 3898 4276 3898
Size: 378 6211
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 2332 2578 3546 3601 5736 1678
Size: 654 246 968 55 2135 2531
Fragments arranged by size:
2531 2135 968 654 246 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3393 3425 3548 3580 3639
Size: 32 135 32 1821 32 123 32 59
Cuts at: 3639 3671 5230 5262 5304 5336 1373
Size: 32 1559 32 42 32 2626
Fragments arranged by size:
2626 1821 1559 135 123 59 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3933 4179 669
Size: 988 281 1995 246 3079
Fragments arranged by size:
3079 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KasI G'GCGC_C
Cuts at: 2328 2328
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 3464 4188 3464
Size: 724 5865
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3603 3783 5703 5887 1305
Size: 2298 180 1920 184 2007
Fragments arranged by size:
2298 2007 1920 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4767
Fragments arranged by size:
4767 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 3662 4547 4906 5065 518
Size: 648 919 1577 885 359 159 2042
Fragments arranged by size:
2042 1577 919 885 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2122 2314 2869 3362 3390 3432 3456
Size: 441 75 192 555 493 28 42 24
Cuts at: 3456 3748 3867 4005 4682 5830 6075 1606
Size: 292 119 138 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 555 493 441 292 245
192 138 119 75 42 28 24
NarI GG'CG_CC
Cuts at: 2329 2329
Size: 6589
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5098
Fragments arranged by size:
5098 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 3947 5492 1132
Size: 97 2718 1545 2229
Fragments arranged by size:
2718 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2323 3211 1348
Size: 975 888 4726
Fragments arranged by size:
4726 975 888
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6589
PfoI T'CCnGG_A
Cuts at: 2631 2631
Size: 6589
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PmlI CAC'GTG
Cuts at: 2556 2803 2556
Size: 247 6342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3003 3003
Size: 6589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 4159 4159
Size: 6589
PspOMI G'GGCC_C
Cuts at: 2040 3730 2040
Size: 1690 4899
PstI C_TGCA'G
Cuts at: 830 3017 3941 830
Size: 2187 924 3478
Fragments arranged by size:
3478 2187 924
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
PvuII CAG'CTG
Cuts at: 2314 2869 3390 3456 2314
Size: 555 521 66 5447
Fragments arranged by size:
5447 555 521 66
RsrII CG'GwC_CG
Cuts at: 2629 2629
Size: 6589
SacI G_AGCT'C
Cuts at: 2227 2268 3102 2227
Size: 41 834 5714
Fragments arranged by size:
5714 834 41
SacII CC_GC'GG
Cuts at: 2048 3433 2048
Size: 1385 5204
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SfcI C'TryA_G
Cuts at: 826 1045 2725 3013 3937 5072 5753 5944 826
Size: 219 1680 288 924 1135 681 191 1471
Fragments arranged by size:
1680 1471 1135 924 681 288 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2748 3659 2748
Size: 911 5678
SfoI GGC'GCC
Cuts at: 2330 2330
Size: 6589
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 2291 2990 3916 1872
Size: 419 699 926 4545
Fragments arranged by size:
4545 926 699 419
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2957 4693 5594 5856 820
Size: 197 926 1014 1736 901 262 1553
Fragments arranged by size:
1736 1553 1014 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3271 4380 493
Size: 2778 1109 2702
Fragments arranged by size:
2778 2702 1109
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 2639 3281 1517
Size: 1122 642 4825
Fragments arranged by size:
4825 1122 642
StyI C'CwwG_G
Cuts at: 513 1065 2004 3195 3337 3375 4028 513
Size: 552 939 1191 142 38 653 3074
Fragments arranged by size:
3074 1191 939 653 552 142 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2097 2709 2803 3329 3499 4616 4633
Size: 1255 517 612 94 526 170 1117 17
Cuts at: 4633 4786 4971 5416 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1117 612 526 517 445 185
170 153 94 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4835 6547 96
Size: 274 80 33 51 1443 2858 1712 138
Fragments arranged by size:
2858 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6549 6581 6549
Size: 32 6557
Tth111I GACn'n_nGTC
Cuts at: 1316 2479 2861 3407 1316
Size: 1163 382 546 4498
Fragments arranged by size:
4498 1163 546 382
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XhoI C'TCGA_G
Cuts at: 1943 2957 1943
Size: 1014 5575
XmaI C'CCGG_G
Cuts at: 1870 2289 2988 3914 1870
Size: 419 699 926 4545
Fragments arranged by size:
4545 926 699 419
XmnI GAAnn'nnTTC
Cuts at: 4718 4718
Size: 6589
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI Bsu36I
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI SanDI SapI SexAI SrfI
SwaI XcmI