Restriction map for
pFN21AB6120
(Circular) (Six-base) MAPSORT of: pFN21AB6120.seq Check: 2821 from: 1 to: 6454
pFN21AB6120 6454 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4264 278
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2154 3797 1937
Size: 217 1643 4594
Fragments arranged by size:
4594 1643 217
Acc65I G'GTAC_C
Cuts at: 3775 3775
Size: 6454
AclI AA'CG_TT
Cuts at: 1563 4581 4954 1563
Size: 3018 373 3063
Fragments arranged by size:
3063 3018 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 2600 3213 3507 3572 4517 5901 6169
Size: 651 317 613 294 65 945 1384 268
Cuts at: 6169 1632
Size: 1917
Fragments arranged by size:
1917 1384 945 651 613 317 294 268
65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6257
AflIII A'CryG_T
Cuts at: 1203 3544 5353 6330 1203
Size: 2341 1809 977 1327
Fragments arranged by size:
2341 1809 1327 977
AgeI A'CCGG_T
Cuts at: 5285 5285
Size: 6454
AhdI GACnn_n'nnGTC
Cuts at: 5183 5183
Size: 6454
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6454
AlwNI CAG_nnn'CTG
Cuts at: 2366 4197 5769 2366
Size: 1831 1572 3051
Fragments arranged by size:
3051 1831 1572
ApaLI G'TGCA_C
Cuts at: 4511 5667 4511
Size: 1156 5298
ApoI r'AATT_y
Cuts at: 1493 2054 2252 2765 3272 3763 3989 4141 6250
Size: 561 198 513 507 491 226 152 2109
Cuts at: 6250 1493
Size: 1697
Fragments arranged by size:
2109 1697 561 513 507 491 226 198
152
AseI AT'TA_AT
Cuts at: 160 1032 2282 5008 160
Size: 872 1250 2726 1606
Fragments arranged by size:
2726 1606 1250 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
AvaI C'yCGr_G
Cuts at: 1870 1943 3779 1870
Size: 73 1836 4545
Fragments arranged by size:
4545 1836 73
BamHI G'GATC_C
Cuts at: 3784 4151 3784
Size: 367 6087
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2794 3775 5230 618
Size: 325 217 627 1007 981 1455 1842
Fragments arranged by size:
1842 1455 1007 981 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3773 1391
Size: 2382 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3372 928
Size: 971 1473 4010
Fragments arranged by size:
4010 1473 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4645 4679 1903
Size: 34 50 34 2624 34 3678
Fragments arranged by size:
3678 2624 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4346 5556 4346
Size: 1210 5244
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6454
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5065 136
Size: 107 122 71 631 605 3393 1525
Fragments arranged by size:
3393 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2593 6449 2593
Size: 3856 2598
BlpI GC'TnA_GC
Cuts at: 2046 3871 2046
Size: 1825 4629
Bme1580I G_kGCm'C
Cuts at: 1790 2968 4515 5671 1790
Size: 1178 1547 1156 2573
Fragments arranged by size:
2573 1547 1178 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4185 5143 442
Size: 416 53 83 399 24 2768 958 1753
Fragments arranged by size:
2768 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3257 1056
Size: 2201 4253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2749 5114 1239
Size: 1510 2365 2579
Fragments arranged by size:
2579 2365 1510
Bpu10I CC'TnA_GC
Cuts at: 2925 2925
Size: 6454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4579 5444 5742 4579
Size: 865 298 5291
Fragments arranged by size:
5291 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5117 882
Size: 617 3618 2219
Fragments arranged by size:
3618 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4245 493
Size: 711 3041 2702
Fragments arranged by size:
3041 2702 711
BsaBI GATnn'nnATC
Cuts at: 6448 6448
Size: 6454
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4261 4643 5278 275
Size: 53 83 186 936 2728 382 635 1451
Fragments arranged by size:
2728 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4887 5285 5559 5706 6117 4887
Size: 398 274 147 411 5224
Fragments arranged by size:
5224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6229
Fragments arranged by size:
6229 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2951 1186
Size: 1765 4689
BseYI C'CCAG_C
Cuts at: 2466 5657 6153 2466
Size: 3191 496 2767
Fragments arranged by size:
3191 2767 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5881
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3904 4665 5693 1540
Size: 275 186 1903 761 1028 2301
Fragments arranged by size:
2301 1903 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4515 4600 5671 4515
Size: 85 1071 5298
Fragments arranged by size:
5298 1071 85
BsmI GAATG_Cn'
Cuts at: 3965 4058 3965
Size: 93 6361
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2968 3773 4515 4600 5671 1391
Size: 399 1178 805 742 85 1071 2174
Fragments arranged by size:
2174 1178 1071 805 742 399 85
BspEI T'CCGG_A
Cuts at: 6117 6117
Size: 6454
BspHI T'CATG_A
Cuts at: 4343 6073 4343
Size: 1730 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3809 844
Size: 2965 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4341 1394
Size: 429 2518 3507
Fragments arranged by size:
3507 2518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3756 4949 5123 65
Size: 1183 2508 1193 174 1396
Fragments arranged by size:
2508 1396 1193 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5098 5285 1700
Size: 110 3288 187 2869
Fragments arranged by size:
3288 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6454
BssSI C'ACGA_G
Cuts at: 4514 5526 4514
Size: 1012 5442
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3206 3266 794
Size: 2412 60 3982
Fragments arranged by size:
3982 2412 60
BstBI TT'CG_AA
Cuts at: 5258 5258
Size: 6454
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6454
BstYI r'GATC_y
Cuts at: 1972 2593 3784 4151 4536 4553 6005 6091 6103
Size: 621 1191 367 385 17 1452 86 12
Cuts at: 6103 6449 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1191 621 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5902
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5715
Fragments arranged by size:
5715 683 56
BtsI GCAGTG_nn'
Cuts at: 3276 3970 4764 4784 3276
Size: 694 794 20 4946
Fragments arranged by size:
4946 794 694 20
ClaI AT'CG_AT
Cuts at: 1554 3735 1554
Size: 2181 4273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6419
DraI TTT'AAA
Cuts at: 3758 3758
Size: 6454
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5461 809
Size: 507 4145 1802
Fragments arranged by size:
4145 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3901 4790 6419
Size: 54 1425 50 275 18 2071 889 1629
Cuts at: 6419 8
Size: 43
Fragments arranged by size:
2071 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3901 1537
Size: 275 2089 4090
Fragments arranged by size:
4090 2089 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 3033 3063 3189 3315 3381 4384 1353
Size: 959 721 30 126 126 66 1003 3423
Fragments arranged by size:
3423 1003 959 721 126 126 66 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5041 5415 5561 387
Size: 1064 138 3452 374 146 1280
Fragments arranged by size:
3452 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2600 2749 3213 3507 3572 4517
Size: 393 651 317 149 464 294 65 945
Cuts at: 4517 5114 5901 6169 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 945 787 651 597 464 393 317
294 268 149 65
EcoRI G'AATT_C
Cuts at: 3272 3763 4141 3272
Size: 491 378 5585
Fragments arranged by size:
5585 491 378
FspI TGC'GCA
Cuts at: 4960 4960
Size: 6454
HaeII r_GCGC'y
Cuts at: 1678 5601 1678
Size: 3923 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2908 2940 3188 3220 3226
Size: 32 135 32 1336 32 248 32 6
Cuts at: 3226 3258 5095 5127 5169 5201 1373
Size: 32 1837 32 42 32 2626
Fragments arranged by size:
2626 1837 1336 248 135 42 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2614 2706 3798 4044 669
Size: 988 281 676 92 1092 246 3079
Fragments arranged by size:
3079 1092 988 676 281 246 92
HindIII A'AGCT_T
Cuts at: 748 3348 748
Size: 2600 3854
HpaI GTT'AAC
Cuts at: 2706 4044 2706
Size: 1338 5116
KpnI G_GTAC'C
Cuts at: 3779 3779
Size: 6454
MfeI C'AATT_G
Cuts at: 4053 4053
Size: 6454
MluI A'CGCG_T
Cuts at: 6330 6330
Size: 6454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2937 3470 5568 5752 1305
Size: 1632 533 2098 184 2007
Fragments arranged by size:
2098 2007 1632 533 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4632
Fragments arranged by size:
4632 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4412 4771 4930 518
Size: 648 3246 359 159 2042
Fragments arranged by size:
3246 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3870 4547 5695 5940 1606
Size: 2264 677 1148 245 2120
Fragments arranged by size:
2264 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5902
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6454
NheI G'CTAG_C
Cuts at: 1052 3253 1052
Size: 2201 4253
NotI GC'GGCC_GC
Cuts at: 3901 3901
Size: 6454
NspI r_CATG'y
Cuts at: 1132 1229 2881 3812 5357 1132
Size: 97 1652 931 1545 2229
Fragments arranged by size:
2229 1652 1545 931 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6454
PciI A'CATG_T
Cuts at: 5353 5353
Size: 6454
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6454
PmeI GTTT'AAAC
Cuts at: 3758 3758
Size: 6454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4523 4555 6036 6068 4523
Size: 32 1481 32 4909
Fragments arranged by size:
4909 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6454
PsiI TTA'TAA
Cuts at: 2774 4024 2774
Size: 1250 5204
PstI C_TGCA'G
Cuts at: 830 2148 2371 3806 830
Size: 1318 223 1435 3478
Fragments arranged by size:
3478 1435 1318 223
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6454
SalI G'TCGA_C
Cuts at: 1936 3796 1936
Size: 1860 4594
SapI GCTCTTCn'nnn_
Cuts at: 3033 3063 3033
Size: 30 6424
SbfI CC_TGCA'GG
Cuts at: 3806 3806
Size: 6454
ScaI AGT'ACT
Cuts at: 4702 6414 4702
Size: 1712 4742
SexAI A'CCwGG_T
Cuts at: 2097 2097
Size: 6454
SfcI C'TryA_G
Cuts at: 826 1045 2144 2367 3802 4937 5618 5809 826
Size: 219 1099 223 1435 1135 681 191 1471
Fragments arranged by size:
1471 1435 1135 1099 681 223 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6454
SmaI CCC'GGG
Cuts at: 1872 3781 1872
Size: 1909 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4558 5459 5721 820
Size: 197 926 2615 901 262 1553
Fragments arranged by size:
2615 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4245 493
Size: 3752 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6454
SphI G_CATG'C
Cuts at: 1132 3812 1132
Size: 2680 3774
SspI AAT'ATT
Cuts at: 5 52 2683 2746 4378 5
Size: 47 2631 63 1632 2081
Fragments arranged by size:
2631 2081 1632 63 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6454
StyI C'CwwG_G
Cuts at: 513 1065 3893 513
Size: 552 2828 3074
Fragments arranged by size:
3074 2828 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4481 4498 4651 4836 5281 325
Size: 1255 2901 17 153 185 445 1498
Fragments arranged by size:
2901 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2908 3687 4700
Size: 274 80 33 51 1443 931 779 1013
Cuts at: 4700 6412 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1013 931 779 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6414 6446 6414
Size: 32 6422
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6454
XbaI T'CTAG_A
Cuts at: 2596 3121 3295 3790 2596
Size: 525 174 495 5260
Fragments arranged by size:
5260 525 495 174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 2402
Size: 6454
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6454
XmaI C'CCGG_G
Cuts at: 1870 3779 1870
Size: 1909 4545
XmnI GAAnn'nnTTC
Cuts at: 2283 2746 4583 2283
Size: 463 1837 4154
Fragments arranged by size:
4154 1837 463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4262 276
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI