Restriction map for
pFN21AB6119
(Circular) (Six-base) MAPSORT of: pFN21AB6119.seq Check: 2168 from: 1 to: 6574
pFN21AB6119 6574 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2823 4384 278
Size: 53 83 186 936 1287 1561 2468
Fragments arranged by size:
2468 1561 1287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2058 4637 6021 6289 1632
Size: 426 2579 1384 268 1917
Fragments arranged by size:
2579 1917 1384 426 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 2017 5473 6450 1203
Size: 814 3456 977 1327
Fragments arranged by size:
3456 1327 977 814
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6574
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 6542
AlwNI CAG_nnn'CTG
Cuts at: 4317 5889 4317
Size: 1572 5002
ApaLI G'TGCA_C
Cuts at: 4631 5787 4631
Size: 1156 5418
ApoI r'AATT_y
Cuts at: 1493 2080 2139 2204 2685 2789 3883 4109 4261
Size: 587 59 65 481 104 1094 226 152
Cuts at: 4261 6370 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1094 587 481 226 152 104
65 59
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 3899 1870
Size: 73 1956 4545
Fragments arranged by size:
4545 1956 73
BamHI G'GATC_C
Cuts at: 2590 3904 4271 2590
Size: 1314 367 4893
Fragments arranged by size:
4893 1314 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3626 3735 3895 5350 618
Size: 325 217 627 1839 109 160 1455 1842
Fragments arranged by size:
1842 1839 1455 627 325 217 160 109
BanII G_rGCy'C
Cuts at: 1391 2046 2750 3764 3893 1391
Size: 655 704 1014 129 4072
Fragments arranged by size:
4072 1014 704 655 129
BbeI G_GCGC'C
Cuts at: 3739 3739
Size: 6574
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2220 2309 928
Size: 971 321 89 5193
Fragments arranged by size:
5193 971 321 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4765 4799 1903
Size: 34 2828 34 3678
Fragments arranged by size:
3678 2828 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2044 3844 4466 5676 2044
Size: 1800 622 1210 2942
Fragments arranged by size:
2942 1800 1210 622
BclI T'GATC_A
Cuts at: 1548 3799 1548
Size: 2251 4323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5185 136
Size: 107 122 71 631 605 3513 1525
Fragments arranged by size:
3513 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2518 6569 2518
Size: 4051 2523
BlpI GC'TnA_GC
Cuts at: 3777 3991 3777
Size: 214 6360
Bme1580I G_kGCm'C
Cuts at: 1790 3631 4635 5791 1790
Size: 1841 1004 1156 2573
Fragments arranged by size:
2573 1841 1156 1004
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3766 4305 5263
Size: 416 53 83 399 24 2349 539 958
Cuts at: 5263 442
Size: 1753
Fragments arranged by size:
2349 1753 958 539 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2433 3360 5234 1239
Size: 1194 927 1874 2579
Fragments arranged by size:
2579 1874 1194 927
Bpu10I CC'TnA_GC
Cuts at: 2652 2652
Size: 6574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4699 5564 5862 4699
Size: 865 298 5411
Fragments arranged by size:
5411 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2609 5237 882
Size: 617 1110 2628 2219
Fragments arranged by size:
2628 2219 1110 617
BsaAI yAC'GTr
Cuts at: 493 1204 3656 4365 493
Size: 711 2452 709 2702
Fragments arranged by size:
2702 2452 711 709
BsaBI GATnn'nnATC
Cuts at: 2991 6568 2991
Size: 3577 2997
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2820 3736 4381 4763
Size: 53 83 186 936 1287 916 645 382
Cuts at: 4763 5398 275
Size: 635 1451
Fragments arranged by size:
1451 1287 936 916 645 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2839 5007 5405 5679 5826 6237 2839
Size: 2168 398 274 147 411 3176
Fragments arranged by size:
3176 2168 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3746 3776 1178
Size: 30 165 30 2343 30 3976
Fragments arranged by size:
3976 2343 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3522 3540 3597 3636 1186
Size: 2336 18 57 39 4124
Fragments arranged by size:
4124 2336 57 39 18
BseYI C'CCAG_C
Cuts at: 5777 6273 5777
Size: 496 6078
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6001
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4635 4720 5791 4635
Size: 85 1071 5418
Fragments arranged by size:
5418 1071 85
BsmI GAATG_Cn'
Cuts at: 4085 4178 4085
Size: 93 6481
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2750 3631 3764 3893 4635 4720
Size: 399 256 704 881 133 129 742 85
Cuts at: 4720 5791 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 881 742 704 399 256 133
129 85
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 2298 2680 4463 6193 2298
Size: 382 1783 1730 2679
Fragments arranged by size:
2679 1783 1730 382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3929 844
Size: 3085 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3787 4461 1394
Size: 429 1964 674 3507
Fragments arranged by size:
3507 1964 674 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2090 3771 5069 5243 65
Size: 1183 842 1681 1298 174 1396
Fragments arranged by size:
1681 1396 1298 1183 842 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3566 5218 5405 1700
Size: 110 1756 1652 187 2869
Fragments arranged by size:
2869 1756 1652 187 110
BsrGI T'GTAC_A
Cuts at: 96 2636 96
Size: 2540 4034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 4634 5646 4634
Size: 1012 5562
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3518 794
Size: 2724 3850
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstYI r'GATC_y
Cuts at: 1972 2508 2518 2572 2590 2986 3451 3904 4271
Size: 536 10 54 18 396 465 453 367
Cuts at: 4271 4656 4673 6125 6211 6223 6569 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 536 465 453 396 385 367
346 86 54 18 17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2997 2997
Size: 6574
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 3556 3646 3673 513
Size: 552 939 42 1510 90 27 3414
Fragments arranged by size:
3414 1510 939 552 90 42 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 4090 4884 4904 4090
Size: 794 20 5760
Fragments arranged by size:
5760 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 2199 2245 3878 2199
Size: 46 1633 4895
Fragments arranged by size:
4895 1633 46
DraIII CAC_nnn'GTG
Cuts at: 2168 2168
Size: 6574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3670 4021 4910
Size: 54 1425 50 275 18 1840 351 889
Cuts at: 4910 6539 8
Size: 1629 43
Fragments arranged by size:
1840 1629 1425 889 351 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 3051 4504 1353
Size: 681 1017 1453 3423
Fragments arranged by size:
3423 1453 1017 681
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5161 5535 5681 387
Size: 1064 138 3572 374 146 1280
Fragments arranged by size:
3572 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2433 3360 4637 5234 6021 6289
Size: 393 426 375 927 1277 597 787 268
Cuts at: 6289 1239
Size: 1524
Fragments arranged by size:
1524 1277 927 787 597 426 393 375
268
EcoO109I rG'GnC_Cy
Cuts at: 3587 3587
Size: 6574
EcoRI G'AATT_C
Cuts at: 2204 3883 4261 2204
Size: 1679 378 4517
Fragments arranged by size:
4517 1679 378
EcoRV GAT'ATC
Cuts at: 3371 3371
Size: 6574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 6574
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 3739 5721 1678
Size: 2061 1982 2531
Fragments arranged by size:
2531 2061 1982
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3571 3603 3733 3765 5215
Size: 32 135 32 1999 32 130 32 1450
Cuts at: 5215 5247 5289 5321 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1999 1450 135 130 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3918 4164 669
Size: 988 281 1980 246 3079
Fragments arranged by size:
3079 1980 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2193 748
Size: 1445 5129
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KasI G'GCGC_C
Cuts at: 3735 3735
Size: 6574
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2935 3353 3463 5688 5872 1305
Size: 1630 418 110 2225 184 2007
Fragments arranged by size:
2225 2007 1630 418 184 110
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4752
Fragments arranged by size:
4752 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2022 2706 3138 4532 4891 5050 518
Size: 648 856 684 432 1394 359 159 2042
Fragments arranged by size:
2042 1394 856 684 648 432 359 159
MspA1I CmG'CkG
Cuts at: 1606 2900 3558 3648 3675 3990 4667 5815 6060
Size: 1294 658 90 27 315 677 1148 245
Cuts at: 6060 1606
Size: 2120
Fragments arranged by size:
2120 1294 1148 677 658 315 245 90
27
NarI GG'CG_CC
Cuts at: 3736 3736
Size: 6574
NcoI C'CATG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 5041
Fragments arranged by size:
5041 939 552 42
NdeI CA'TA_TG
Cuts at: 387 3318 387
Size: 2931 3643
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2021 3932 5477 1132
Size: 97 792 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 792 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6574
PciI A'CATG_T
Cuts at: 2017 5473 2017
Size: 3456 3118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2600 3461 1108
Size: 1492 861 4221
Fragments arranged by size:
4221 1492 861
PfoI T'CCnGG_A
Cuts at: 3589 3589
Size: 6574
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 4643 4675 6156 6188 3746
Size: 32 865 32 1481 32 4132
Fragments arranged by size:
4132 1481 865 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3587 3587
Size: 6574
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PstI C_TGCA'G
Cuts at: 830 2065 3926 830
Size: 1235 1861 3478
Fragments arranged by size:
3478 1861 1235
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2900 2900
Size: 6574
SacII CC_GC'GG
Cuts at: 3559 3649 3676 3559
Size: 90 27 6457
Fragments arranged by size:
6457 90 27
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SapI GCTCTTCn'nnn_
Cuts at: 2034 2034
Size: 6574
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SfcI C'TryA_G
Cuts at: 826 1045 2010 2061 2979 3922 5057 5738 5929
Size: 219 965 51 918 943 1135 681 191
Cuts at: 5929 826
Size: 1471
Fragments arranged by size:
1471 1135 965 943 918 681 219 191
51
SfoI GGC'GCC
Cuts at: 3737 3737
Size: 6574
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3901 1872
Size: 2029 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4678 5579 5841 820
Size: 197 926 2735 901 262 1553
Fragments arranged by size:
2735 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3932 1132
Size: 2800 3774
SspI AAT'ATT
Cuts at: 5 52 3205 4498 5
Size: 47 3153 1293 2081
Fragments arranged by size:
3153 2081 1293 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6574
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 2769 3790 4013 513
Size: 552 939 42 723 1021 223 3074
Fragments arranged by size:
3074 1021 939 723 552 223 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4601 4618 4771 4956 5401 325
Size: 1255 3021 17 153 185 445 1498
Fragments arranged by size:
3021 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2636 4820 6532
Size: 274 80 33 51 1443 659 2184 1712
Cuts at: 6532 96
Size: 138
Fragments arranged by size:
2184 1712 1443 659 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6534 6566 6534
Size: 32 6542
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3434 3434
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 3899 1870
Size: 2029 4545
XmnI GAAnn'nnTTC
Cuts at: 2082 2538 2778 3474 4703 2082
Size: 456 240 696 1229 3953
Fragments arranged by size:
3953 1229 696 456 240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2821 4382 276
Size: 53 83 186 936 1287 1561 2468
Fragments arranged by size:
2468 1561 1287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI
SwaI