Restriction map for
pFN21AB6115
(Circular) (Six-base) MAPSORT of: pFN21AB6115.seq Check: 8490 from: 1 to: 6565
pFN21AB6115 6565 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2647 2647
Size: 6565
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4375 278
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3908 1937
Size: 1971 4594
Acc65I G'GTAC_C
Cuts at: 3886 3886
Size: 6565
AclI AA'CG_TT
Cuts at: 1563 4692 5065 1563
Size: 3129 373 3063
Fragments arranged by size:
3129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2746 3616 4628 6012 6280 1632
Size: 1114 870 1012 1384 268 1917
Fragments arranged by size:
1917 1384 1114 1012 870 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6368
AflIII A'CryG_T
Cuts at: 1203 5464 6441 1203
Size: 4261 977 1327
Fragments arranged by size:
4261 1327 977
AgeI A'CCGG_T
Cuts at: 5396 5396
Size: 6565
AhdI GACnn_n'nnGTC
Cuts at: 5294 5294
Size: 6565
AleI CACnn'nnGTG
Cuts at: 1166 2954 1166
Size: 1788 4777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6565
AlwNI CAG_nnn'CTG
Cuts at: 2769 3325 3591 4308 5880 2769
Size: 556 266 717 1572 3454
Fragments arranged by size:
3454 1572 717 556 266
ApaI G_GGCC'C
Cuts at: 2081 2081
Size: 6565
ApaLI G'TGCA_C
Cuts at: 4622 5778 4622
Size: 1156 5409
ApoI r'AATT_y
Cuts at: 1493 3874 4100 4252 6361 1493
Size: 2381 226 152 2109 1697
Fragments arranged by size:
2381 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5119 160
Size: 872 4087 1606
Fragments arranged by size:
4087 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
AvaI C'yCGr_G
Cuts at: 1870 1943 2608 3794 3890 1870
Size: 73 665 1186 96 4545
Fragments arranged by size:
4545 1186 665 96 73
BamHI G'GATC_C
Cuts at: 2535 3895 4262 2535
Size: 1360 367 4838
Fragments arranged by size:
4838 1360 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2291 3718 3886 5341 618
Size: 325 217 627 504 1427 168 1455 1842
Fragments arranged by size:
1842 1455 1427 627 504 325 217 168
BanII G_rGCy'C
Cuts at: 1391 2067 2081 2097 2165 2529 3306 3345 3884
Size: 676 14 16 68 364 777 39 539
Cuts at: 3884 1391
Size: 4072
Fragments arranged by size:
4072 777 676 539 364 68 39 16
14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 2789 3088 3226 3409 928
Size: 971 132 758 299 138 183 4084
Fragments arranged by size:
4084 971 758 299 183 138 132
BbvCI CC'TCA_GC
Cuts at: 3217 3217
Size: 6565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2185 2219 4756 4790 1903
Size: 34 248 34 2537 34 3678
Fragments arranged by size:
3678 2537 248 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4457 5667 4457
Size: 1210 5355
BclI T'GATC_A
Cuts at: 1548 3294 1548
Size: 1746 4819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5176 136
Size: 107 122 71 631 605 3504 1525
Fragments arranged by size:
3504 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2403 6560 2403
Size: 4157 2408
BlpI GC'TnA_GC
Cuts at: 3982 3982
Size: 6565
Bme1580I G_kGCm'C
Cuts at: 1790 2081 3138 3723 4626 5782 1790
Size: 291 1057 585 903 1156 2573
Fragments arranged by size:
2573 1156 1057 903 585 291
BmgBI CAC'GTC
Cuts at: 1318 3148 3450 1318
Size: 1830 302 4433
Fragments arranged by size:
4433 1830 302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3679 4296 5254
Size: 416 53 83 399 24 2262 617 958
Cuts at: 5254 442
Size: 1753
Fragments arranged by size:
2262 1753 958 617 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6565
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 2144
Size: 6565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2578 2889 2986 5225 1239
Size: 1339 311 97 2239 2579
Fragments arranged by size:
2579 2239 1339 311 97
Bpu10I CC'TnA_GC
Cuts at: 2058 2897 3217 2058
Size: 839 320 5406
Fragments arranged by size:
5406 839 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3842 4690 5555 5853 3842
Size: 848 865 298 4554
Fragments arranged by size:
4554 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 5228 882
Size: 617 681 3048 2219
Fragments arranged by size:
3048 2219 681 617
BsaAI yAC'GTr
Cuts at: 493 1204 2827 4356 493
Size: 711 1623 1529 2702
Fragments arranged by size:
2702 1623 1529 711
BsaBI GATnn'nnATC
Cuts at: 2580 3176 6559 2580
Size: 596 3383 2586
Fragments arranged by size:
3383 2586 596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2927 4372 4754 5389
Size: 53 83 186 936 1394 1445 382 635
Cuts at: 5389 275
Size: 1451
Fragments arranged by size:
1451 1445 1394 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4998 5396 5670 5817 6228 4998
Size: 398 274 147 411 5335
Fragments arranged by size:
5335 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6340
Fragments arranged by size:
6340 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2500 3070 1186
Size: 1314 570 4681
Fragments arranged by size:
4681 1314 570
BseYI C'CCAG_C
Cuts at: 2330 3551 3682 3687 5768 6264 2330
Size: 1221 131 5 2081 496 2631
Fragments arranged by size:
2631 2081 1221 496 131 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2480 2654 3311 1163
Size: 573 408 336 174 657 4417
Fragments arranged by size:
4417 657 573 408 336 174
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4015 4776 5804 1540
Size: 275 186 2014 761 1028 2301
Fragments arranged by size:
2301 2014 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 2529 3345 3471 4626 4711 5782 2097
Size: 432 816 126 1155 85 1071 2880
Fragments arranged by size:
2880 1155 1071 816 432 126 85
BsmI GAATG_Cn'
Cuts at: 4076 4169 4076
Size: 93 6472
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2081 2097 2165 2529 3138 3306
Size: 399 277 14 16 68 364 609 168
Cuts at: 3306 3345 3471 3723 3884 4626 4711 5782 1391
Size: 39 126 252 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 609 399 364 277 252
168 161 126 85 68 39 16 14
BspEI T'CCGG_A
Cuts at: 6228 6228
Size: 6565
BspHI T'CATG_A
Cuts at: 4454 6184 4454
Size: 1730 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2319 2647 3920 844
Size: 1475 328 1273 3489
Fragments arranged by size:
3489 1475 1273 328
BsrBI CCG'CTC
Cuts at: 1394 1823 4452 1394
Size: 429 2629 3507
Fragments arranged by size:
3507 2629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2325 3786 5060 5234 65
Size: 1183 1077 1461 1274 174 1396
Fragments arranged by size:
1461 1396 1274 1183 1077 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2913 3443 5209 5396 1700
Size: 110 1103 530 1766 187 2869
Fragments arranged by size:
2869 1766 1103 530 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6565
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6565
BssSI C'ACGA_G
Cuts at: 4625 5637 4625
Size: 1012 5553
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3133 794
Size: 2339 4226
BstBI TT'CG_AA
Cuts at: 5369 5369
Size: 6565
BstEII G'GTnAC_C
Cuts at: 1184 2857 1184
Size: 1673 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 2074 2687 3487 3682 3715 2074
Size: 613 800 195 33 4924
Fragments arranged by size:
4924 800 613 195 33
BstYI r'GATC_y
Cuts at: 1972 2403 2535 3895 4262 4647 4664 6116 6202
Size: 431 132 1360 367 385 17 1452 86
Cuts at: 6202 6214 6560 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1360 431 385 367 346 132
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2788 2788
Size: 6565
BtgI C'CryG_G
Cuts at: 513 1065 2025 2043 2073 2153 2166 2520 2686
Size: 552 960 18 30 80 13 354 166
Cuts at: 2686 2711 3228 3273 3708 3714 513
Size: 25 517 45 435 6 3364
Fragments arranged by size:
3364 960 552 517 435 354 166 80
45 30 25 18 13 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2467 3181 3679 487
Size: 683 56 1241 714 498 3373
Fragments arranged by size:
3373 1241 714 683 498 56
BtsI GCAGTG_nn'
Cuts at: 2539 2835 4081 4875 4895 2539
Size: 296 1246 794 20 4209
Fragments arranged by size:
4209 1246 794 296 20
ClaI AT'CG_AT
Cuts at: 1554 3309 1554
Size: 1755 4810
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2266 2301 529
Size: 35 1702 35 4793
Fragments arranged by size:
4793 1702 35 35
DraI TTT'AAA
Cuts at: 3869 3869
Size: 6565
DraIII CAC_nnn'GTG
Cuts at: 3133 3133
Size: 6565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3044 5572 809
Size: 507 1728 2528 1802
Fragments arranged by size:
2528 1802 1728 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 2365 2946
Size: 54 1425 50 275 18 414 121 581
Cuts at: 2946 3150 3711 4012 4901 6530 8
Size: 204 561 301 889 1629 43
Fragments arranged by size:
1629 1425 889 581 561 414 301 275
204 121 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4012 1537
Size: 275 2200 4090
Fragments arranged by size:
4090 2200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 2510 3350 3359 4495 1353
Size: 968 189 840 9 1136 3423
Fragments arranged by size:
3423 1136 968 840 189 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3836 5152 5526 5672 387
Size: 1064 138 2247 1316 374 146 1280
Fragments arranged by size:
2247 1316 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2095 2527 3343 2095
Size: 432 816 5317
Fragments arranged by size:
5317 816 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2578 2746 2889 2986 3616 4628 5225
Size: 393 946 168 143 97 630 1012 597
Cuts at: 5225 6012 6280 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1012 946 787 630 597 393 268
168 143 97
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 2771 2105
Size: 666 5899
EcoO109I rG'GnC_Cy
Cuts at: 2705 3759 2705
Size: 1054 5511
EcoRI G'AATT_C
Cuts at: 3874 4252 3874
Size: 378 6187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 6565
FspI TGC'GCA
Cuts at: 5071 5071
Size: 6565
HaeII r_GCGC'y
Cuts at: 1678 2495 3273 5712 1678
Size: 817 778 2439 2531
Fragments arranged by size:
2531 2439 817 778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3456 3488 3642 3674 5206
Size: 32 135 32 1884 32 154 32 1532
Cuts at: 5206 5238 5280 5312 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1884 1532 154 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2905 3909 4155 669
Size: 988 281 967 1004 246 3079
Fragments arranged by size:
3079 1004 988 967 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6565
HpaI GTT'AAC
Cuts at: 4155 4155
Size: 6565
KpnI G_GTAC'C
Cuts at: 3890 3890
Size: 6565
MfeI C'AATT_G
Cuts at: 4164 4164
Size: 6565
MluI A'CGCG_T
Cuts at: 6441 6441
Size: 6565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5679 5863 1305
Size: 4374 184 2007
Fragments arranged by size:
4374 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 3152 3713 10
Size: 54 1425 343 414 906 561 2862
Fragments arranged by size:
2862 1425 906 561 414 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2586 2954 3485 3713 4523 4882 5041
Size: 648 1420 368 531 228 810 359 159
Cuts at: 5041 518
Size: 2042
Fragments arranged by size:
2042 1420 810 648 531 368 359 228
159
MspA1I CmG'CkG
Cuts at: 1606 2155 2390 2713 3322 3547 3981 4658 5806
Size: 549 235 323 609 225 434 677 1148
Cuts at: 5806 6051 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 609 549 434 323 245
235 225
NaeI GCC'GGC
Cuts at: 3445 3445
Size: 6565
NcoI C'CATG_G
Cuts at: 513 1065 2686 3273 3708 3714 513
Size: 552 1621 587 435 6 3364
Fragments arranged by size:
3364 1621 587 552 435 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6565
NgoMIV G'CCGG_C
Cuts at: 3443 3443
Size: 6565
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6565
NotI GC'GGCC_GC
Cuts at: 4012 4012
Size: 6565
NspI r_CATG'y
Cuts at: 1132 1229 3923 5468 1132
Size: 97 2694 1545 2229
Fragments arranged by size:
2694 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2968 3256 1348
Size: 1620 288 4657
Fragments arranged by size:
4657 1620 288
PciI A'CATG_T
Cuts at: 5464 5464
Size: 6565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2253 1108
Size: 1145 5420
PfoI T'CCnGG_A
Cuts at: 2552 3426 2552
Size: 874 5691
PmeI GTTT'AAAC
Cuts at: 3869 3869
Size: 6565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4634 4666 6147 6179 4634
Size: 32 1481 32 5020
Fragments arranged by size:
5020 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3759 3759
Size: 6565
PshAI GACnn'nnGTC
Cuts at: 1613 2035 1613
Size: 422 6143
PsiI TTA'TAA
Cuts at: 4135 4135
Size: 6565
PspOMI G'GGCC_C
Cuts at: 2077 2077
Size: 6565
PstI C_TGCA'G
Cuts at: 830 3749 3917 830
Size: 2919 168 3478
Fragments arranged by size:
3478 2919 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6565
PvuII CAG'CTG
Cuts at: 3322 3322
Size: 6565
SacI G_AGCT'C
Cuts at: 2097 2529 3345 2097
Size: 432 816 5317
Fragments arranged by size:
5317 816 432
SacII CC_GC'GG
Cuts at: 2156 2714 2156
Size: 558 6007
SalI G'TCGA_C
Cuts at: 1936 3907 1936
Size: 1971 4594
SapI GCTCTTCn'nnn_
Cuts at: 2321 3350 3359 2321
Size: 1029 9 5527
Fragments arranged by size:
5527 1029 9
SbfI CC_TGCA'GG
Cuts at: 3917 3917
Size: 6565
ScaI AGT'ACT
Cuts at: 2651 4813 6525 2651
Size: 2162 1712 2691
Fragments arranged by size:
2691 2162 1712
SfcI C'TryA_G
Cuts at: 826 1045 3031 3745 3913 5048 5729 5920 826
Size: 219 1986 714 168 1135 681 191 1471
Fragments arranged by size:
1986 1471 1135 714 681 219 191 168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6565
SmaI CCC'GGG
Cuts at: 1872 3892 1872
Size: 2020 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3857 4669 5570 5832 820
Size: 197 926 1914 812 901 262 1553
Fragments arranged by size:
1914 1553 926 901 812 262 197
SnaBI TAC'GTA
Cuts at: 493 4356 493
Size: 3863 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6565
SphI G_CATG'C
Cuts at: 1132 3923 1132
Size: 2791 3774
SspI AAT'ATT
Cuts at: 5 52 4489 5
Size: 47 4437 2081
Fragments arranged by size:
4437 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6565
StyI C'CwwG_G
Cuts at: 513 1065 2686 3153 3273 3708 3714 4004 513
Size: 552 1621 467 120 435 6 290 3074
Fragments arranged by size:
3074 1621 552 467 435 290 120 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2966 3266 4592 4609 4762 4947 5392
Size: 1255 1386 300 1326 17 153 185 445
Cuts at: 5392 325
Size: 1498
Fragments arranged by size:
1498 1386 1326 1255 445 300 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 3558 4811
Size: 274 80 33 51 1443 672 909 1253
Cuts at: 4811 6523 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1253 909 672 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6525 6557 6525
Size: 32 6533
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6565
XbaI T'CTAG_A
Cuts at: 3901 3901
Size: 6565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2684 2824 3145 3280 3706 2684
Size: 140 321 135 426 5543
Fragments arranged by size:
5543 426 321 140 135
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6565
XmaI C'CCGG_G
Cuts at: 1870 3890 1870
Size: 2020 4545
XmnI GAAnn'nnTTC
Cuts at: 4694 4694
Size: 6565
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4373 276
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI
SfiI SfoI SrfI SwaI