Restriction map for
pFN21AB6113
(Circular) (Six-base) MAPSORT of: pFN21AB6113.seq Check: 5729 from: 1 to: 6565
pFN21AB6113 6565 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4375 278
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3765 3908 1937
Size: 1828 143 4594
Fragments arranged by size:
4594 1828 143
Acc65I G'GTAC_C
Cuts at: 3886 3886
Size: 6565
AclI AA'CG_TT
Cuts at: 1563 4692 5065 1563
Size: 3129 373 3063
Fragments arranged by size:
3129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 2508 2538 3246 4628 6012 6280 1632
Size: 545 331 30 708 1382 1384 268 1917
Fragments arranged by size:
1917 1384 1382 708 545 331 268 30
AfeI AGC'GCT
Cuts at: 2306 2306
Size: 6565
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6368
AflIII A'CryG_T
Cuts at: 1203 3760 5464 6441 1203
Size: 2557 1704 977 1327
Fragments arranged by size:
2557 1704 1327 977
AgeI A'CCGG_T
Cuts at: 5396 5396
Size: 6565
AhdI GACnn_n'nnGTC
Cuts at: 5294 5294
Size: 6565
AleI CACnn'nnGTG
Cuts at: 1166 3098 1166
Size: 1932 4633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6565
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2386 2418 2386
Size: 32 6533
AlwNI CAG_nnn'CTG
Cuts at: 4308 5880 4308
Size: 1572 4993
ApaLI G'TGCA_C
Cuts at: 4622 5778 4622
Size: 1156 5409
ApoI r'AATT_y
Cuts at: 1493 3560 3874 4100 4252 6361 1493
Size: 2067 314 226 152 2109 1697
Fragments arranged by size:
2109 2067 1697 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5119 160
Size: 872 4087 1606
Fragments arranged by size:
4087 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
AvaI C'yCGr_G
Cuts at: 1870 1943 3890 1870
Size: 73 1947 4545
Fragments arranged by size:
4545 1947 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2993 3026 3703 3736 2993
Size: 33 677 33 5822
Fragments arranged by size:
5822 677 33 33
BamHI G'GATC_C
Cuts at: 3895 4262 3895
Size: 367 6198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2353 3554 3886 5341 618
Size: 325 217 627 566 1201 332 1455 1842
Fragments arranged by size:
1842 1455 1201 627 566 332 325 217
BanII G_rGCy'C
Cuts at: 1391 2175 3093 3884 1391
Size: 784 918 791 4072
Fragments arranged by size:
4072 918 791 784
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2496 3717 928
Size: 971 597 1221 3776
Fragments arranged by size:
3776 1221 971 597
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4756 4790 1903
Size: 34 2819 34 3678
Fragments arranged by size:
3678 2819 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3021 4457 5667 3021
Size: 1436 1210 3919
Fragments arranged by size:
3919 1436 1210
BclI T'GATC_A
Cuts at: 1548 3532 1548
Size: 1984 4581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2117 5176 136
Size: 107 122 71 631 605 445 3059 1525
Fragments arranged by size:
3059 1525 631 605 445 122 107 71
BglII A'GATC_T
Cuts at: 6560 6560
Size: 6565
BlpI GC'TnA_GC
Cuts at: 3982 3982
Size: 6565
Bme1580I G_kGCm'C
Cuts at: 1790 2437 4626 5782 1790
Size: 647 2189 1156 2573
Fragments arranged by size:
2573 2189 1156 647
BmgBI CAC'GTC
Cuts at: 1318 3763 1318
Size: 2445 4120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3035 4296 5254
Size: 416 53 83 399 24 1618 1261 958
Cuts at: 5254 442
Size: 1753
Fragments arranged by size:
1753 1618 1261 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6565
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3610 3610
Size: 6565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2268 2432 3302 3805 5225 1239
Size: 1029 164 870 503 1420 2579
Fragments arranged by size:
2579 1420 1029 870 503 164
Bpu10I CC'TnA_GC
Cuts at: 2910 2910
Size: 6565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4690 5555 5853 4690
Size: 865 298 5402
Fragments arranged by size:
5402 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3368 3790 5228 882
Size: 617 1869 422 1438 2219
Fragments arranged by size:
2219 1869 1438 617 422
BsaAI yAC'GTr
Cuts at: 493 1204 4356 493
Size: 711 3152 2702
Fragments arranged by size:
3152 2702 711
BsaBI GATnn'nnATC
Cuts at: 6559 6559
Size: 6565
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4372 4754 5389 275
Size: 53 83 186 936 2839 382 635 1451
Fragments arranged by size:
2839 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3006 3722 4998 5396 5670 5817 6228 3006
Size: 716 1276 398 274 147 411 3343
Fragments arranged by size:
3343 1276 716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2386 2416 2979 3009 1178
Size: 30 165 30 983 30 563 30 4734
Fragments arranged by size:
4734 983 563 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3000 3233 1186
Size: 1814 233 4518
Fragments arranged by size:
4518 1814 233
BseYI C'CCAG_C
Cuts at: 2307 3351 3382 5768 6264 2307
Size: 1044 31 2386 496 2608
Fragments arranged by size:
2608 2386 1044 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3281 1163
Size: 573 1545 4447
Fragments arranged by size:
4447 1545 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4015 4776 5804 1540
Size: 275 186 2014 761 1028 2301
Fragments arranged by size:
2301 2014 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 3093 3324 4626 4711 5782 2175
Size: 918 231 1302 85 1071 2958
Fragments arranged by size:
2958 1302 1071 918 231 85
BsmI GAATG_Cn'
Cuts at: 2461 4076 4169 2461
Size: 1615 93 4857
Fragments arranged by size:
4857 1615 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2437 3093 3324 3884 4626 4711
Size: 399 385 262 656 231 560 742 85
Cuts at: 4711 5782 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 656 560 399 385 262
231 85
BspEI T'CCGG_A
Cuts at: 6228 6228
Size: 6565
BspHI T'CATG_A
Cuts at: 4454 6184 4454
Size: 1730 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3920 844
Size: 3076 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4452 1394
Size: 429 2629 3507
Fragments arranged by size:
3507 2629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 5060 5234 65
Size: 1183 1284 2528 174 1396
Fragments arranged by size:
2528 1396 1284 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2298 5209 5396 1700
Size: 110 488 2911 187 2869
Fragments arranged by size:
2911 2869 488 187 110
BsrGI T'GTAC_A
Cuts at: 96 3735 96
Size: 3639 2926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6565
BssSI C'ACGA_G
Cuts at: 4625 5637 4625
Size: 1012 5553
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2223 794
Size: 1429 5136
BstBI TT'CG_AA
Cuts at: 5369 5369
Size: 6565
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6565
BstXI CCAn_nnnn'nTGG
Cuts at: 2981 3301 2981
Size: 320 6245
BstYI r'GATC_y
Cuts at: 1972 3895 4262 4647 4664 6116 6202 6214 6560
Size: 1923 367 385 17 1452 86 12 346
Cuts at: 6560 1972
Size: 1977
Fragments arranged by size:
1977 1923 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2565 2565
Size: 6565
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5826
Fragments arranged by size:
5826 683 56
BtsI GCAGTG_nn'
Cuts at: 2576 3121 3781 4081 4875 4895 2576
Size: 545 660 300 794 20 4246
Fragments arranged by size:
4246 794 660 545 300 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6565
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6530
DraI TTT'AAA
Cuts at: 2083 3869 2083
Size: 1786 4779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3831 5572 809
Size: 507 2515 1741 1802
Fragments arranged by size:
2515 1802 1741 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3809 4012 4901
Size: 54 1425 50 275 18 1979 203 889
Cuts at: 4901 6530 8
Size: 1629 43
Fragments arranged by size:
1979 1629 1425 889 275 203 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4012 1537
Size: 275 2200 4090
Fragments arranged by size:
4090 2200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 2586 2961 3198 3420 4495 1353
Size: 1125 108 375 237 222 1075 3423
Fragments arranged by size:
3423 1125 1075 375 237 222 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5152 5526 5672 387
Size: 1064 138 3563 374 146 1280
Fragments arranged by size:
3563 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2173 3091 2173
Size: 918 5647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2268 2432 2508 2538 3246 3302
Size: 393 545 91 164 76 30 708 56
Cuts at: 3302 3805 4628 5225 6012 6280 1239
Size: 503 823 597 787 268 1524
Fragments arranged by size:
1524 823 787 708 597 545 503 393
268 164 91 76 56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3284 3425 3284
Size: 141 6424
EcoO109I rG'GnC_Cy
Cuts at: 3289 3289
Size: 6565
EcoRI G'AATT_C
Cuts at: 3874 4252 3874
Size: 378 6187
FspI TGC'GCA
Cuts at: 5071 5071
Size: 6565
HaeII r_GCGC'y
Cuts at: 1678 2308 5712 1678
Size: 630 3404 2531
Fragments arranged by size:
3404 2531 630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3614 3646 3763 3795 5206
Size: 32 135 32 2042 32 117 32 1411
Cuts at: 5206 5238 5280 5312 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2042 1411 135 117 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2103 3836 3909 4155 669
Size: 988 281 165 1733 73 246 3079
Fragments arranged by size:
3079 1733 988 281 246 165 73
HindIII A'AGCT_T
Cuts at: 748 2417 748
Size: 1669 4896
HpaI GTT'AAC
Cuts at: 2103 4155 2103
Size: 2052 4513
KpnI G_GTAC'C
Cuts at: 3890 3890
Size: 6565
MfeI C'AATT_G
Cuts at: 4164 4164
Size: 6565
MluI A'CGCG_T
Cuts at: 6441 6441
Size: 6565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2471 5679 5863 1305
Size: 1166 3208 184 2007
Fragments arranged by size:
3208 2007 1166 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4743
Fragments arranged by size:
4743 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3098 3494 4523 4882 5041 518
Size: 648 1932 396 1029 359 159 2042
Fragments arranged by size:
2042 1932 1029 648 396 359 159
MspA1I CmG'CkG
Cuts at: 1606 2410 3265 3355 3981 4658 5806 6051 1606
Size: 804 855 90 626 677 1148 245 2120
Fragments arranged by size:
2120 1148 855 804 677 626 245 90
NaeI GCC'GGC
Cuts at: 2300 2300
Size: 6565
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6013
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6565
NgoMIV G'CCGG_C
Cuts at: 2298 2298
Size: 6565
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6565
NotI GC'GGCC_GC
Cuts at: 4012 4012
Size: 6565
NspI r_CATG'y
Cuts at: 1132 1229 3391 3435 3923 5468 1132
Size: 97 2162 44 488 1545 2229
Fragments arranged by size:
2229 2162 1545 488 97 44
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6565
PciI A'CATG_T
Cuts at: 5464 5464
Size: 6565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6565
PfoI T'CCnGG_A
Cuts at: 2022 2637 2804 2022
Size: 615 167 5783
Fragments arranged by size:
5783 615 167
PmeI GTTT'AAAC
Cuts at: 3869 3869
Size: 6565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2418 4634 4666 6147 6179 2386
Size: 32 2216 32 1481 32 2772
Fragments arranged by size:
2772 2216 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6565
PsiI TTA'TAA
Cuts at: 4135 4135
Size: 6565
PstI C_TGCA'G
Cuts at: 830 2815 3373 3917 830
Size: 1985 558 544 3478
Fragments arranged by size:
3478 1985 558 544
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6565
PvuII CAG'CTG
Cuts at: 2410 3265 2410
Size: 855 5710
SacI G_AGCT'C
Cuts at: 2175 3093 2175
Size: 918 5647
SalI G'TCGA_C
Cuts at: 1936 3907 1936
Size: 1971 4594
SbfI CC_TGCA'GG
Cuts at: 3917 3917
Size: 6565
ScaI AGT'ACT
Cuts at: 2597 4813 6525 2597
Size: 2216 1712 2637
Fragments arranged by size:
2637 2216 1712
SfcI C'TryA_G
Cuts at: 826 1045 2811 3369 3607 3913 5048 5729 5920
Size: 219 1766 558 238 306 1135 681 191
Cuts at: 5920 826
Size: 1471
Fragments arranged by size:
1766 1471 1135 681 558 306 238 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6565
SmaI CCC'GGG
Cuts at: 1872 3892 1872
Size: 2020 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4669 5570 5832 820
Size: 197 926 2726 901 262 1553
Fragments arranged by size:
2726 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4356 493
Size: 3863 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6565
SphI G_CATG'C
Cuts at: 1132 3923 1132
Size: 2791 3774
SspI AAT'ATT
Cuts at: 5 52 4489 5
Size: 47 4437 2081
Fragments arranged by size:
4437 2081 47
StuI AGG'CCT
Cuts at: 1517 2991 1517
Size: 1474 5091
StyI C'CwwG_G
Cuts at: 513 1065 2841 4004 513
Size: 552 1776 1163 3074
Fragments arranged by size:
3074 1776 1163 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2299 3061 3365 4592 4609 4762 4947
Size: 1255 719 762 304 1227 17 153 185
Cuts at: 4947 5392 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1227 762 719 445 304 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3537 3735
Size: 274 80 33 51 1443 618 942 198
Cuts at: 3735 4811 6523 96
Size: 1076 1712 138
Fragments arranged by size:
1712 1443 1076 942 618 274 198 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 2979 3011 6525 6557 2375
Size: 32 572 32 3514 32 2383
Fragments arranged by size:
3514 2383 572 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6565
XbaI T'CTAG_A
Cuts at: 3901 3901
Size: 6565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2645 3466 2113
Size: 532 821 5212
Fragments arranged by size:
5212 821 532
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6565
XmaI C'CCGG_G
Cuts at: 1870 3890 1870
Size: 2020 4545
XmnI GAAnn'nnTTC
Cuts at: 2065 2757 4694 2065
Size: 692 1937 3936
Fragments arranged by size:
3936 1937 692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4373 276
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI