Restriction map for
pFN21AB6112
(Circular) (Six-base) MAPSORT of: pFN21AB6112.seq Check: 8413 from: 1 to: 6562
pFN21AB6112 6562 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3432 4372 278
Size: 53 83 186 936 1896 940 2468
Fragments arranged by size:
2468 1896 940 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3447 3905 1937
Size: 1510 458 4594
Fragments arranged by size:
4594 1510 458
Acc65I G'GTAC_C
Cuts at: 3883 3883
Size: 6562
AclI AA'CG_TT
Cuts at: 1563 3387 4689 5062 1563
Size: 1824 1302 373 3063
Fragments arranged by size:
3063 1824 1302 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 2903 2969 3389 3648 3755 4625 6009
Size: 1004 267 66 420 259 107 870 1384
Cuts at: 6009 6277 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1004 870 420 268 267 259
107 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6365
AflIII A'CryG_T
Cuts at: 1203 2047 2850 3377 5461 6438 1203
Size: 844 803 527 2084 977 1327
Fragments arranged by size:
2084 1327 977 844 803 527
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 3517 5291 3517
Size: 1774 4788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6562
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3607 3639 3607
Size: 32 6530
AlwNI CAG_nnn'CTG
Cuts at: 2757 4305 5877 2757
Size: 1548 1572 3442
Fragments arranged by size:
3442 1572 1548
ApaI G_GGCC'C
Cuts at: 2482 2482
Size: 6562
ApaLI G'TGCA_C
Cuts at: 4619 5775 4619
Size: 1156 5406
ApoI r'AATT_y
Cuts at: 1493 3871 4097 4249 6358 1493
Size: 2378 226 152 2109 1697
Fragments arranged by size:
2378 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 2208 3522 3749 3887 1870
Size: 73 265 1314 227 138 4545
Fragments arranged by size:
4545 1314 265 227 138 73
BamHI G'GATC_C
Cuts at: 3892 4259 3892
Size: 367 6195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2330 2872 3682 3883 5338
Size: 325 217 627 543 542 810 201 1455
Cuts at: 5338 618
Size: 1842
Fragments arranged by size:
1842 1455 810 627 543 542 325 217
201
BanII G_rGCy'C
Cuts at: 1391 2308 2482 2964 3222 3881 1391
Size: 917 174 482 258 659 4072
Fragments arranged by size:
4072 917 659 482 258 174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2142 2502 3490 928
Size: 971 243 360 988 4000
Fragments arranged by size:
4000 988 971 360 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 4753 4787 1903
Size: 34 1793 34 989 34 3678
Fragments arranged by size:
3678 1793 989 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4454 5664 4454
Size: 1210 5352
BclI T'GATC_A
Cuts at: 1548 2646 1548
Size: 1098 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3060 5173 136
Size: 107 122 71 631 605 1388 2113 1525
Fragments arranged by size:
2113 1525 1388 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3797 6557 3797
Size: 2760 3802
BlpI GC'TnA_GC
Cuts at: 3298 3979 3298
Size: 681 5881
Bme1580I G_kGCm'C
Cuts at: 1790 2442 2482 3687 4623 5779 1790
Size: 652 40 1205 936 1156 2573
Fragments arranged by size:
2573 1205 1156 936 652 40
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2476 3727 3793
Size: 416 53 83 399 24 1059 1251 66
Cuts at: 3793 4293 5251 442
Size: 500 958 1753
Fragments arranged by size:
1753 1251 1059 958 500 416 399 83
66 53 24
BmtI G_CTAG'C
Cuts at: 1056 3573 1056
Size: 2517 4045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 3225 3658 3681 5222 1239
Size: 1056 930 433 23 1541 2579
Fragments arranged by size:
2579 1541 1056 930 433 23
Bpu10I CC'TnA_GC
Cuts at: 2411 2799 3257 2411
Size: 388 458 5716
Fragments arranged by size:
5716 458 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2839 3806 4687 5552 5850 2180
Size: 659 967 881 865 298 2892
Fragments arranged by size:
2892 967 881 865 659 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2994 3201 5225 882
Size: 617 1495 207 2024 2219
Fragments arranged by size:
2219 2024 1495 617 207
BsaAI yAC'GTr
Cuts at: 493 1204 4353 493
Size: 711 3149 2702
Fragments arranged by size:
3149 2702 711
BsaBI GATnn'nnATC
Cuts at: 2271 2567 6556 2271
Size: 296 3989 2277
Fragments arranged by size:
3989 2277 296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2499 3429 4369 4751
Size: 53 83 186 936 966 930 940 382
Cuts at: 4751 5386 275
Size: 635 1451
Fragments arranged by size:
1451 966 940 936 930 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2091 4995 5393 5667 5814 6225 2091
Size: 2904 398 274 147 411 2428
Fragments arranged by size:
2904 2428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2654 2684 3204 3234 3679
Size: 30 165 30 1251 30 520 30 445
Cuts at: 3679 3709 1178
Size: 30 4031
Fragments arranged by size:
4031 1251 520 445 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2298 2334 1186
Size: 1112 36 5414
Fragments arranged by size:
5414 1112 36
BseYI C'CCAG_C
Cuts at: 3420 5765 6261 3420
Size: 2345 496 3721
Fragments arranged by size:
3721 2345 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2452 3642 1163
Size: 573 716 1190 4083
Fragments arranged by size:
4083 1190 716 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4012 4773 5801 1540
Size: 275 186 2011 761 1028 2301
Fragments arranged by size:
2301 2011 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2308 2964 4623 4708 5779 2308
Size: 656 1659 85 1071 3091
Fragments arranged by size:
3091 1659 1071 656 85
BsmI GAATG_Cn'
Cuts at: 2277 3261 3622 3722 3851 4073 4166 2277
Size: 984 361 100 129 222 93 4673
Fragments arranged by size:
4673 984 361 222 129 100 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3386 3386
Size: 6562
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2308 2442 2482 2964 3222 3687 3881
Size: 399 518 134 40 482 258 465 194
Cuts at: 3881 4623 4708 5779 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 518 482 465 399 258
194 134 85 40
BspEI T'CCGG_A
Cuts at: 6225 6225
Size: 6562
BspHI T'CATG_A
Cuts at: 2649 3855 4451 6181 2649
Size: 1206 596 1730 3030
Fragments arranged by size:
3030 1730 1206 596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3917 844
Size: 3073 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2923 4449 1394
Size: 429 1100 1526 3507
Fragments arranged by size:
3507 1526 1100 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2190 3470 3555 5057 5231 65
Size: 1183 942 1280 85 1502 174 1396
Fragments arranged by size:
1502 1396 1280 1183 942 174 85
BsrFI r'CCGG_y
Cuts at: 1700 1810 5206 5393 1700
Size: 110 3396 187 2869
Fragments arranged by size:
3396 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2835 96
Size: 2739 3823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6562
BssSI C'ACGA_G
Cuts at: 4622 5634 4622
Size: 1012 5550
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3175 794
Size: 2381 4181
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6562
BstYI r'GATC_y
Cuts at: 1972 2886 3633 3797 3837 3892 4259 4644 4661
Size: 914 747 164 40 55 367 385 17
Cuts at: 4661 6113 6199 6211 6557 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 914 747 385 367 346 164
86 55 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 2774 2774
Size: 6562
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5071
Fragments arranged by size:
5071 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5823
Fragments arranged by size:
5823 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 4078 4872 4892 2440
Size: 1638 794 20 4110
Fragments arranged by size:
4110 1638 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 3866 3866
Size: 6562
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2244 5569 809
Size: 507 928 3325 1802
Fragments arranged by size:
3325 1802 928 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3061 4009 4898
Size: 54 1425 50 275 18 1231 948 889
Cuts at: 4898 6527 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1231 948 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4009 1537
Size: 275 2197 4090
Fragments arranged by size:
4090 2197 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2122 2587 2781 2952 3149 3624 4492 1353
Size: 769 465 194 171 197 475 868 3423
Fragments arranged by size:
3423 868 769 475 465 197 194 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5149 5523 5669 387
Size: 1064 138 435 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2306 2962 2306
Size: 656 5906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2295 2636 2903 2969 3225 3389 3648
Size: 393 663 341 267 66 256 164 259
Cuts at: 3648 3658 3681 3755 4625 5222 6009 6277 1239
Size: 10 23 74 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 663 597 393 341 268
267 259 256 164 74 66 23 10
EcoO109I rG'GnC_Cy
Cuts at: 2084 2346 3243 2084
Size: 262 897 5403
Fragments arranged by size:
5403 897 262
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
EcoRV GAT'ATC
Cuts at: 2565 2565
Size: 6562
FspI TGC'GCA
Cuts at: 5068 5068
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 3024 3459 5709 1678
Size: 1346 435 2250 2531
Fragments arranged by size:
2531 2250 1346 435
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2333 2365 2530
Size: 32 135 32 434 32 295 32 165
Cuts at: 2530 2562 2792 2824 2837 2869 2963 2995 3204
Size: 32 230 32 13 32 94 32 209
Cuts at: 3204 3236 3503 3535 5203 5235 5277 5309 1373
Size: 32 267 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 434 295 267 230 209 165
135 94 42 32 32 32 32 32
32 32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 3906 4152 669
Size: 988 281 592 1376 246 3079
Fragments arranged by size:
3079 1376 988 592 281 246
HindIII A'AGCT_T
Cuts at: 748 3335 748
Size: 2587 3975
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KpnI G_GTAC'C
Cuts at: 3887 3887
Size: 6562
MfeI C'AATT_G
Cuts at: 3081 4161 3081
Size: 1080 5482
MluI A'CGCG_T
Cuts at: 2047 3377 6438 2047
Size: 1330 3061 2171
Fragments arranged by size:
3061 2171 1330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5676 5860 1305
Size: 4371 184 2007
Fragments arranged by size:
4371 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3063 10
Size: 54 1425 343 1231 3509
Fragments arranged by size:
3509 1425 1231 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2040 3475 4520 4879 5038 518
Size: 648 874 1435 1045 359 159 2042
Fragments arranged by size:
2042 1435 1045 874 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3978 4655 5803 6048 1606
Size: 2372 677 1148 245 2120
Fragments arranged by size:
2372 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5071
Fragments arranged by size:
5071 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6562
NheI G'CTAG_C
Cuts at: 1052 3569 1052
Size: 2517 4045
NotI GC'GGCC_GC
Cuts at: 4009 4009
Size: 6562
NruI TCG'CGA
Cuts at: 3030 3030
Size: 6562
NspI r_CATG'y
Cuts at: 1132 1229 2854 3920 5465 1132
Size: 97 1625 1066 1545 2229
Fragments arranged by size:
2229 1625 1545 1066 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6562
PciI A'CATG_T
Cuts at: 2850 5461 2850
Size: 2611 3951
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6562
PfoI T'CCnGG_A
Cuts at: 3222 3222
Size: 6562
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2084 2346 2084
Size: 262 6300
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PspOMI G'GGCC_C
Cuts at: 2478 2478
Size: 6562
PspXI vC'TCGA_Gb
Cuts at: 3749 3749
Size: 6562
PstI C_TGCA'G
Cuts at: 830 3914 830
Size: 3084 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
SacI G_AGCT'C
Cuts at: 2308 2964 2308
Size: 656 5906
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SanDI GG'GwC_CC
Cuts at: 2084 2084
Size: 6562
SapI GCTCTTCn'nnn_
Cuts at: 2952 2952
Size: 6562
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 2506 3185 4810 6522 2506
Size: 679 1625 1712 2546
Fragments arranged by size:
2546 1712 1625 679
SfcI C'TryA_G
Cuts at: 826 1045 3910 5045 5726 5917 826
Size: 219 2865 1135 681 191 1471
Fragments arranged by size:
2865 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6562
SmaI CCC'GGG
Cuts at: 1872 3524 3889 1872
Size: 1652 365 4545
Fragments arranged by size:
4545 1652 365
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 2818 3749 3821 4666 5567
Size: 197 926 216 659 931 72 845 901
Cuts at: 5567 5829 820
Size: 262 1553
Fragments arranged by size:
1553 931 926 901 845 659 262 216
197 72
SnaBI TAC'GTA
Cuts at: 493 4353 493
Size: 3860 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 3920 1132
Size: 2788 3774
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 2697 3809 1517
Size: 1180 1112 4270
Fragments arranged by size:
4270 1180 1112
StyI C'CwwG_G
Cuts at: 513 1065 2004 3828 4001 513
Size: 552 939 1824 173 3074
Fragments arranged by size:
3074 1824 939 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2136 2890 3225 3676 4589 4606 4759
Size: 1255 556 754 335 451 913 17 153
Cuts at: 4759 4944 5389 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 913 754 556 451 445 335
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2504 2835 3183
Size: 274 80 33 51 1443 527 331 348
Cuts at: 3183 4808 6520 96
Size: 1625 1712 138
Fragments arranged by size:
1712 1625 1443 527 348 331 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2089 2121 3679 3711 6522 6554 2089
Size: 32 1558 32 2811 32 2097
Fragments arranged by size:
2811 2097 1558 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2738 1316
Size: 1422 5140
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XhoI C'TCGA_G
Cuts at: 1943 3749 1943
Size: 1806 4756
XmaI C'CCGG_G
Cuts at: 1870 3522 3887 1870
Size: 1652 365 4545
Fragments arranged by size:
4545 1652 365
XmnI GAAnn'nnTTC
Cuts at: 2360 3036 4691 2360
Size: 676 1655 4231
Fragments arranged by size:
4231 1655 676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3430 4370 276
Size: 53 83 186 936 1896 940 2468
Fragments arranged by size:
2468 1896 940 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI
PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI
SwaI XcmI