Restriction map for
pFN21AB6106
(Circular) (Six-base) MAPSORT of: pFN21AB6106.seq Check: 9998 from: 1 to: 6508
pFN21AB6106 6508 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3851 1937
Size: 1914 4594
Acc65I G'GTAC_C
Cuts at: 3829 3829
Size: 6508
AclI AA'CG_TT
Cuts at: 1563 3807 4635 5008 1563
Size: 2244 828 373 3063
Fragments arranged by size:
3063 2244 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 4571 5955 6223 1632
Size: 876 2063 1384 268 1917
Fragments arranged by size:
2063 1917 1384 876 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 5407 6384 1203
Size: 4204 977 1327
Fragments arranged by size:
4204 1327 977
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 3595 5237 3595
Size: 1642 4866
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2837 1184
Size: 1653 4855
AlwNI CAG_nnn'CTG
Cuts at: 2977 3361 4251 5823 2977
Size: 384 890 1572 3662
Fragments arranged by size:
3662 1572 890 384
ApaLI G'TGCA_C
Cuts at: 4565 5721 4565
Size: 1156 5352
ApoI r'AATT_y
Cuts at: 1493 2559 2676 3817 4043 4195 6304 1493
Size: 1066 117 1141 226 152 2109 1697
Fragments arranged by size:
2109 1697 1141 1066 226 152 117
AseI AT'TA_AT
Cuts at: 160 1032 5062 160
Size: 872 4030 1606
Fragments arranged by size:
4030 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 3566 3833 1870
Size: 73 1359 264 267 4545
Fragments arranged by size:
4545 1359 267 264 73
BamHI G'GATC_C
Cuts at: 2620 3045 3838 4205 2620
Size: 425 793 367 4923
Fragments arranged by size:
4923 793 425 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3829 5284 618
Size: 325 217 627 2042 1455 1842
Fragments arranged by size:
2042 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3827 1391
Size: 2436 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 3594 3663 3699 3720 928
Size: 971 162 1533 69 36 21 3716
Fragments arranged by size:
3716 1533 971 162 69 36 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4699 4733 1903
Size: 34 2762 34 3678
Fragments arranged by size:
3678 2762 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5119 136
Size: 107 122 71 631 605 3447 1525
Fragments arranged by size:
3447 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6503 6503
Size: 6508
BlpI GC'TnA_GC
Cuts at: 2130 3365 3925 2130
Size: 1235 560 4713
Fragments arranged by size:
4713 1235 560
Bme1580I G_kGCm'C
Cuts at: 1790 4569 5725 1790
Size: 2779 1156 2573
Fragments arranged by size:
2779 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3104 3612 1318
Size: 1786 508 4214
Fragments arranged by size:
4214 1786 508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2035 4239 5197
Size: 416 53 83 399 24 618 2204 958
Cuts at: 5197 442
Size: 1753
Fragments arranged by size:
2204 1753 958 618 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2837 5168 1239
Size: 1598 2331 2579
Fragments arranged by size:
2579 2331 1598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4633 5498 5796 4633
Size: 865 298 5345
Fragments arranged by size:
5345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2303 5171 882
Size: 617 804 2868 2219
Fragments arranged by size:
2868 2219 804 617
BsaAI yAC'GTr
Cuts at: 493 1204 4299 493
Size: 711 3095 2702
Fragments arranged by size:
3095 2702 711
BsaBI GATnn'nnATC
Cuts at: 3399 6502 3399
Size: 3103 3405
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 4315 4697 5332
Size: 53 83 186 936 1762 1020 382 635
Cuts at: 5332 275
Size: 1451
Fragments arranged by size:
1762 1451 1020 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4941 5339 5613 5760 6171 4941
Size: 398 274 147 411 5278
Fragments arranged by size:
5278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6283
Fragments arranged by size:
6283 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2540 1186
Size: 1354 5154
BseYI C'CCAG_C
Cuts at: 3351 5711 6207 3351
Size: 2360 496 3652
Fragments arranged by size:
3652 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5935
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3958 4719 5747 1540
Size: 275 186 1957 761 1028 2301
Fragments arranged by size:
2301 1957 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4569 4654 5725 4569
Size: 85 1071 5352
Fragments arranged by size:
5352 1071 85
BsmI GAATG_Cn'
Cuts at: 2159 2903 4019 4112 2159
Size: 744 1116 93 4555
Fragments arranged by size:
4555 1116 744 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3827 4569 4654 5725 1391
Size: 399 2037 742 85 1071 2174
Fragments arranged by size:
2174 2037 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 2722 4397 6127 2722
Size: 1675 1730 3103
Fragments arranged by size:
3103 1730 1675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3863 844
Size: 3019 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4395 1394
Size: 429 2572 3507
Fragments arranged by size:
3507 2572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5003 5177 65
Size: 1183 3755 174 1396
Fragments arranged by size:
3755 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5152 5339 1700
Size: 110 3342 187 2869
Fragments arranged by size:
3342 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3459 96
Size: 3363 3145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 3479 4568 5580 3479
Size: 1089 1012 4407
Fragments arranged by size:
4407 1089 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3361 794
Size: 2567 3941
BstBI TT'CG_AA
Cuts at: 5312 5312
Size: 6508
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 2620 3045 3838 4205 4590 4607 6059 6145
Size: 648 425 793 367 385 17 1452 86
Cuts at: 6145 6157 6503 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 793 648 425 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3194 3194
Size: 6508
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5017
Fragments arranged by size:
5017 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2477 487
Size: 683 56 1251 4518
Fragments arranged by size:
4518 1251 683 56
BtsI GCAGTG_nn'
Cuts at: 4024 4818 4838 4024
Size: 794 20 5694
Fragments arranged by size:
5694 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6473
DraI TTT'AAA
Cuts at: 2947 3115 3812 2947
Size: 168 697 5643
Fragments arranged by size:
5643 697 168
DraIII CAC_nnn'GTG
Cuts at: 2455 2455
Size: 6508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5515 809
Size: 507 4199 1802
Fragments arranged by size:
4199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3225 3483 3525
Size: 54 1425 50 275 18 1395 258 42
Cuts at: 3525 3955 4844 6473 8
Size: 430 889 1629 43
Fragments arranged by size:
1629 1425 1395 889 430 275 258 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3955 1537
Size: 275 2143 4090
Fragments arranged by size:
4090 2143 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2484 2517 4438 1353
Size: 1131 33 1921 3423
Fragments arranged by size:
3423 1921 1131 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2870 5095 5469 5615 387
Size: 1064 138 1281 2225 374 146 1280
Fragments arranged by size:
2225 1281 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 2837 4571 5168 5955 6223 1239
Size: 393 876 329 1734 597 787 268 1524
Fragments arranged by size:
1734 1524 876 787 597 393 329 268
EcoO109I rG'GnC_Cy
Cuts at: 2122 2122
Size: 6508
EcoRI G'AATT_C
Cuts at: 2676 3817 4195 2676
Size: 1141 378 4989
Fragments arranged by size:
4989 1141 378
EcoRV GAT'ATC
Cuts at: 2720 2720
Size: 6508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3203 3737 3203
Size: 534 5974
FspI TGC'GCA
Cuts at: 5014 5014
Size: 6508
HaeII r_GCGC'y
Cuts at: 1678 5655 1678
Size: 3977 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2307 2339 3285 3317 3501
Size: 32 135 32 735 32 946 32 184
Cuts at: 3501 3526 3533 3558 3581 3613 3752 3784 5149
Size: 25 7 25 23 32 139 32 1365
Cuts at: 5149 5181 5223 5255 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1365 946 735 184 139 135 42
32 32 32 32 32 32 32 32
25 25 23 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3852 4098 669
Size: 988 281 1914 246 3079
Fragments arranged by size:
3079 1914 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6508
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KpnI G_GTAC'C
Cuts at: 3833 3833
Size: 6508
MfeI C'AATT_G
Cuts at: 2205 4107 2205
Size: 1902 4606
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2286 2763 5622 5806 1305
Size: 981 477 2859 184 2007
Fragments arranged by size:
2859 2007 981 477 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4686
Fragments arranged by size:
4686 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4466 4825 4984 518
Size: 648 3300 359 159 2042
Fragments arranged by size:
3300 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2857 2893 3523 3577 3924 4601 5749 5994
Size: 1251 36 630 54 347 677 1148 245
Cuts at: 5994 1606
Size: 2120
Fragments arranged by size:
2120 1251 1148 677 630 347 245 54
36
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5017
Fragments arranged by size:
5017 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6508
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 3866 5411 1132
Size: 97 2637 1545 2229
Fragments arranged by size:
2637 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6508
PciI A'CATG_T
Cuts at: 5407 5407
Size: 6508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6508
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4609 6090 6122 4577
Size: 32 1481 32 4963
Fragments arranged by size:
4963 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2122
Size: 6508
PshAI GACnn'nnGTC
Cuts at: 1613 3417 1613
Size: 1804 4704
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PstI C_TGCA'G
Cuts at: 830 3860 830
Size: 3030 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6508
PvuII CAG'CTG
Cuts at: 2857 2893 3523 2857
Size: 36 630 5842
Fragments arranged by size:
5842 630 36
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SbfI CC_TGCA'GG
Cuts at: 3860 3860
Size: 6508
ScaI AGT'ACT
Cuts at: 2138 4756 6468 2138
Size: 2618 1712 2178
Fragments arranged by size:
2618 2178 1712
SfcI C'TryA_G
Cuts at: 826 1045 3856 4991 5672 5863 826
Size: 219 2811 1135 681 191 1471
Fragments arranged by size:
2811 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 3835 1872
Size: 1963 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3302 4612 5513 5775 820
Size: 197 926 1359 1310 901 262 1553
Fragments arranged by size:
1553 1359 1310 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4299 493
Size: 3806 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3866 1132
Size: 2734 3774
SspI AAT'ATT
Cuts at: 5 52 2798 4432 5
Size: 47 2746 1634 2081
Fragments arranged by size:
2746 2081 1634 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6508
StyI C'CwwG_G
Cuts at: 513 1065 2004 3947 513
Size: 552 939 1943 3074
Fragments arranged by size:
3074 1943 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3465 4535 4552 4705 4890 5335 325
Size: 1255 1885 1070 17 153 185 445 1498
Fragments arranged by size:
1885 1498 1255 1070 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 3459 4754
Size: 274 80 33 51 1443 159 1323 1295
Cuts at: 4754 6466 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1323 1295 274 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2852 2884 6468 6500 2852
Size: 32 3584 32 2860
Fragments arranged by size:
3584 2860 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6508
XbaI T'CTAG_A
Cuts at: 3844 3844
Size: 6508
XhoI C'TCGA_G
Cuts at: 1943 3302 1943
Size: 1359 5149
XmaI C'CCGG_G
Cuts at: 1870 3833 1870
Size: 1963 4545
XmnI GAAnn'nnTTC
Cuts at: 2507 3696 4637 2507
Size: 1189 941 4378
Fragments arranged by size:
4378 1189 941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI