Restriction map for
pFN21AB6102
(Circular) (Six-base) MAPSORT of: pFN21AB6102.seq Check: 7024 from: 1 to: 6547
pFN21AB6102 6547 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3820 3820
Size: 6547
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3890 1937
Size: 1953 4594
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2891 4610 5994 6262 1632
Size: 1259 1719 1384 268 1917
Fragments arranged by size:
1917 1719 1384 1259 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6350
AflIII A'CryG_T
Cuts at: 1203 5446 6423 1203
Size: 4243 977 1327
Fragments arranged by size:
4243 1327 977
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 5276 5276
Size: 6547
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2862 1184
Size: 1678 4869
AlwNI CAG_nnn'CTG
Cuts at: 2644 3594 3673 4290 5862 2644
Size: 950 79 617 1572 3329
Fragments arranged by size:
3329 1572 950 617 79
ApaLI G'TGCA_C
Cuts at: 2720 4604 5760 2720
Size: 1884 1156 3507
Fragments arranged by size:
3507 1884 1156
ApoI r'AATT_y
Cuts at: 1493 2504 3381 3856 4082 4234 6343 1493
Size: 1011 877 475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1011 877 475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 2613 2873 3872 1870
Size: 73 670 260 999 4545
Fragments arranged by size:
4545 999 670 260 73
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 2279 3648 3868 5323
Size: 325 217 627 326 166 1369 220 1455
Cuts at: 5323 618
Size: 1842
Fragments arranged by size:
1842 1455 1369 627 326 325 220 217
166
BanII G_rGCy'C
Cuts at: 1391 3689 3731 3866 1391
Size: 2298 42 135 4072
Fragments arranged by size:
4072 2298 135 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5576
BbvCI CC'TCA_GC
Cuts at: 3221 3417 3221
Size: 196 6351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2594 2628 3777 3811 4738 4772 1903
Size: 34 657 34 1149 34 927 34 3678
Fragments arranged by size:
3678 1149 927 657 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4439 5649 3320
Size: 1119 1210 4218
Fragments arranged by size:
4218 1210 1119
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6547
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3693 5158 136
Size: 107 122 71 631 605 2021 1465 1525
Fragments arranged by size:
2021 1525 1465 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6542 6542
Size: 6547
BlpI GC'TnA_GC
Cuts at: 2649 3964 2649
Size: 1315 5232
Bme1580I G_kGCm'C
Cuts at: 1790 2284 2724 2862 3762 4608 5764 1790
Size: 494 440 138 900 846 1156 2573
Fragments arranged by size:
2573 1156 900 846 494 440 138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2632 2736 3649
Size: 416 53 83 399 24 1215 104 913
Cuts at: 3649 4278 5236 442
Size: 629 958 1753
Fragments arranged by size:
1753 1215 958 913 629 416 399 104
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2223 3232 5207 1239
Size: 984 1009 1975 2579
Fragments arranged by size:
2579 1975 1009 984
Bpu10I CC'TnA_GC
Cuts at: 2793 3221 3417 2793
Size: 428 196 5923
Fragments arranged by size:
5923 428 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2552 4672 5537 5835 2552
Size: 2120 865 298 3264
Fragments arranged by size:
3264 2120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3250 5210 882
Size: 617 1751 1960 2219
Fragments arranged by size:
2219 1960 1751 617
BsaAI yAC'GTr
Cuts at: 493 1204 3331 4338 493
Size: 711 2127 1007 2702
Fragments arranged by size:
2702 2127 1007 711
BsaBI GATnn'nnATC
Cuts at: 6541 6541
Size: 6547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4354 4736 5371 275
Size: 53 83 186 936 2821 382 635 1451
Fragments arranged by size:
2821 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4980 5378 5652 5799 6210 4980
Size: 398 274 147 411 5317
Fragments arranged by size:
5317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2773 2803 1178
Size: 30 165 30 1370 30 4922
Fragments arranged by size:
4922 1370 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2253 2311 2767 2823 3551 3721 1186
Size: 1067 58 456 56 728 170 4012
Fragments arranged by size:
4012 1067 728 456 170 58 56
BseYI C'CCAG_C
Cuts at: 2084 3761 3766 5750 6246 2084
Size: 1677 5 1984 496 2385
Fragments arranged by size:
2385 1984 1677 496 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2319 2588 3291 1163
Size: 573 583 269 703 4419
Fragments arranged by size:
4419 703 583 573 269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3707 3997 4758 5786 1540
Size: 275 186 1706 290 761 1028 2301
Fragments arranged by size:
2301 1706 1028 761 290 275 186
BsiHKAI G_wGCw'C
Cuts at: 2374 2724 3463 4608 4693 5764 2374
Size: 350 739 1145 85 1071 3157
Fragments arranged by size:
3157 1145 1071 739 350 85
BsmI GAATG_Cn'
Cuts at: 4058 4151 4058
Size: 93 6454
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2374 2724 2862 3463 3689 3731
Size: 399 494 90 350 138 601 226 42
Cuts at: 3731 3762 3866 4608 4693 5764 1391
Size: 31 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 494 399 350 226
138 104 90 85 42 31
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 2865 4436 6166 2865
Size: 1571 1730 3246
Fragments arranged by size:
3246 1730 1571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2586 3681 3820 3902 844
Size: 1742 1095 139 82 3489
Fragments arranged by size:
3489 1742 1095 139 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3646 4434 1394
Size: 429 1823 788 3507
Fragments arranged by size:
3507 1823 788 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2251 2314 2431 2679 3292 5042 5216
Size: 1183 1003 63 117 248 613 1750 174
Cuts at: 5216 65
Size: 1396
Fragments arranged by size:
1750 1396 1183 1003 613 248 174 117
63
BsrFI r'CCGG_y
Cuts at: 1700 1810 3702 5191 5378 1700
Size: 110 1892 1489 187 2869
Fragments arranged by size:
2869 1892 1489 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2690 3509 3594 794
Size: 1896 819 85 3747
Fragments arranged by size:
3747 1896 819 85
BstBI TT'CG_AA
Cuts at: 5351 5351
Size: 6547
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6547
BstXI CCAn_nnnn'nTGG
Cuts at: 2084 3005 2084
Size: 921 5626
BstYI r'GATC_y
Cuts at: 1972 2159 3877 4244 4629 4646 6098 6184 6196
Size: 187 1718 367 385 17 1452 86 12
Cuts at: 6196 6542 1972
Size: 346 1977
Fragments arranged by size:
1977 1718 1452 385 367 346 187 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2050 2050
Size: 6547
BtgI C'CryG_G
Cuts at: 513 1065 2004 3543 3691 513
Size: 552 939 1539 148 3369
Fragments arranged by size:
3369 1539 939 552 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2135 2670 3780 487
Size: 683 56 909 535 1110 3254
Fragments arranged by size:
3254 1110 909 683 535 56
BtsI GCAGTG_nn'
Cuts at: 2605 3595 4063 4857 4877 2605
Size: 990 468 794 20 4275
Fragments arranged by size:
4275 990 794 468 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6512
DraI TTT'AAA
Cuts at: 3851 3851
Size: 6547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3000 5554 809
Size: 507 1684 2554 1802
Fragments arranged by size:
2554 1802 1684 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2619 3565
Size: 54 1425 50 275 18 193 596 946
Cuts at: 3565 3679 3694 3704 3994 4883 6512 8
Size: 114 15 10 290 889 1629 43
Fragments arranged by size:
1629 1425 946 889 596 290 275 193
114 54 50 43 18 15 10
EagI C'GGCC_G
Cuts at: 1537 1812 3704 3994 1537
Size: 275 1892 290 4090
Fragments arranged by size:
4090 1892 290 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2729 2754 2910 4477 1353
Size: 1376 25 156 1567 3423
Fragments arranged by size:
3423 1567 1376 156 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3733 5134 5508 5654 387
Size: 1064 138 2144 1401 374 146 1280
Fragments arranged by size:
2144 1401 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2891 3232 4610 5207 5994 6262
Size: 393 591 668 341 1378 597 787 268
Cuts at: 6262 1239
Size: 1524
Fragments arranged by size:
1524 1378 787 668 597 591 393 341
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3736 3736
Size: 6547
EcoRI G'AATT_C
Cuts at: 2504 3856 4234 2504
Size: 1352 378 4817
Fragments arranged by size:
4817 1352 378
FseI GG_CCGG'CC
Cuts at: 3706 3706
Size: 6547
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 3136 3157 5694 1678
Size: 1458 21 2537 2531
Fragments arranged by size:
2537 2531 1458 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2728 2760 3767 3799 5188
Size: 32 135 32 1156 32 1007 32 1389
Cuts at: 5188 5220 5262 5294 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1389 1156 1007 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3891 4137 669
Size: 988 281 1953 246 3079
Fragments arranged by size:
3079 1953 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6547
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 2351 2496 2784 4146 2351
Size: 145 288 1362 4752
Fragments arranged by size:
4752 1362 288 145
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5661 5845 1305
Size: 4356 184 2007
Fragments arranged by size:
4356 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2025 3681 10
Size: 54 1425 343 193 1656 2876
Fragments arranged by size:
2876 1656 1425 343 193 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2717 2727 4505 4864 5023 518
Size: 648 1551 10 1778 359 159 2042
Fragments arranged by size:
2042 1778 1551 648 359 159 10
MspA1I CmG'CkG
Cuts at: 1606 2593 3709 3963 4640 5788 6033 1606
Size: 987 1116 254 677 1148 245 2120
Fragments arranged by size:
2120 1148 1116 987 677 254 245
NaeI GCC'GGC
Cuts at: 3704 3704
Size: 6547
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5056
Fragments arranged by size:
5056 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NgoMIV G'CCGG_C
Cuts at: 3702 3702
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 3588 3905 5450 1132
Size: 97 2359 317 1545 2229
Fragments arranged by size:
2359 2229 1545 317 97
PasI CC'CwG_GG
Cuts at: 1348 3263 1348
Size: 1915 4632
PciI A'CATG_T
Cuts at: 5446 5446
Size: 6547
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 1108
Size: 1536 5011
PfoI T'CCnGG_A
Cuts at: 2200 2200
Size: 6547
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PstI C_TGCA'G
Cuts at: 830 2592 3755 3899 830
Size: 1762 1163 144 3478
Fragments arranged by size:
3478 1762 1163 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SbfI CC_TGCA'GG
Cuts at: 3899 3899
Size: 6547
ScaI AGT'ACT
Cuts at: 4795 6507 4795
Size: 1712 4835
SfcI C'TryA_G
Cuts at: 826 1045 2588 3717 3751 3895 5030 5711 5902
Size: 219 1543 1129 34 144 1135 681 191
Cuts at: 5902 826
Size: 1471
Fragments arranged by size:
1543 1471 1135 1129 681 219 191 144
34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 2875 3874 1872
Size: 1003 999 4545
Fragments arranged by size:
4545 1003 999
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 4651 5552 5814 820
Size: 197 926 624 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 624 262 197
SnaBI TAC'GTA
Cuts at: 493 3331 4338 493
Size: 2838 1007 2702
Fragments arranged by size:
2838 2702 1007
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 3905 1132
Size: 2773 3774
SspI AAT'ATT
Cuts at: 5 52 4471 5
Size: 47 4419 2081
Fragments arranged by size:
4419 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6547
StyI C'CwwG_G
Cuts at: 513 1065 2004 3986 513
Size: 552 939 1982 3074
Fragments arranged by size:
3074 1982 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2224 3251 4574 4591 4744 4929 5374
Size: 1255 644 1027 1323 17 153 185 445
Cuts at: 5374 325
Size: 1498
Fragments arranged by size:
1498 1323 1255 1027 644 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3400 4793 6505
Size: 274 80 33 51 1443 1423 1393 1712
Cuts at: 6505 96
Size: 138
Fragments arranged by size:
1712 1443 1423 1393 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 2521 2553 2773 2805 6507 6539 2121
Size: 32 368 32 220 32 3702 32 2129
Fragments arranged by size:
3702 2129 368 220 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6547
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3659 3659
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6547
XmaI C'CCGG_G
Cuts at: 1870 2873 3872 1870
Size: 1003 999 4545
Fragments arranged by size:
4545 1003 999
XmnI GAAnn'nnTTC
Cuts at: 2661 4676 2661
Size: 2015 4532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI