Restriction map for
pFN21AB6017
(Circular) (Six-base) MAPSORT of: pFN21AB6017.seq Check: 768 from: 1 to: 7186
pFN21AB6017 7186 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4134 4134
Size: 7186
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4996 278
Size: 53 83 186 936 3460 2468
Fragments arranged by size:
3460 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3441 4529 1937
Size: 1504 1088 4594
Fragments arranged by size:
4594 1504 1088
Acc65I G'GTAC_C
Cuts at: 2784 4507 2784
Size: 1723 5463
AclI AA'CG_TT
Cuts at: 1563 5313 5686 1563
Size: 3750 373 3063
Fragments arranged by size:
3750 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3739 4285 4301 4409 5249 6633 6901 1632
Size: 2107 546 16 108 840 1384 268 1917
Fragments arranged by size:
2107 1917 1384 840 546 268 108 16
AfeI AGC'GCT
Cuts at: 2404 2404
Size: 7186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6989
AflIII A'CryG_T
Cuts at: 1203 4428 6085 7062 1203
Size: 3225 1657 977 1327
Fragments arranged by size:
3225 1657 1327 977
AgeI A'CCGG_T
Cuts at: 6017 6017
Size: 7186
AhdI GACnn_n'nnGTC
Cuts at: 5915 5915
Size: 7186
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7186
AlwNI CAG_nnn'CTG
Cuts at: 2198 3004 4929 6501 2198
Size: 806 1925 1572 2883
Fragments arranged by size:
2883 1925 1572 806
ApaI G_GGCC'C
Cuts at: 3373 3373
Size: 7186
ApaLI G'TGCA_C
Cuts at: 3449 5243 6399 3449
Size: 1794 1156 4236
Fragments arranged by size:
4236 1794 1156
ApoI r'AATT_y
Cuts at: 1493 4495 4721 4873 6982 1493
Size: 3002 226 152 2109 1697
Fragments arranged by size:
3002 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5740 160
Size: 872 4708 1606
Fragments arranged by size:
4708 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7186
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 2849 3160 3823 4511 1870
Size: 73 894 12 311 663 688 4545
Fragments arranged by size:
4545 894 688 663 311 73 12
BamHI G'GATC_C
Cuts at: 3075 4516 4883 3075
Size: 1441 367 5378
Fragments arranged by size:
5378 1441 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 2279 2436 2516 2657
Size: 325 217 627 417 75 157 80 141
Cuts at: 2657 2784 2939 3746 4507 5962 618
Size: 127 155 807 761 1455 1842
Fragments arranged by size:
1842 1455 807 761 627 417 325 217
157 155 141 127 80 75
BanII G_rGCy'C
Cuts at: 1391 2087 2636 3373 3696 3767 4320 4505 1391
Size: 696 549 737 323 71 553 185 4072
Fragments arranged by size:
4072 737 696 553 549 323 185 71
BbeI G_GCGC'C
Cuts at: 2440 2440
Size: 7186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2281 3919 3998 4087 928
Size: 971 382 1638 79 89 4027
Fragments arranged by size:
4027 1638 971 382 89 79
BbvCI CC'TCA_GC
Cuts at: 2934 2934
Size: 7186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5377 5411 1903
Size: 34 3440 34 3678
Fragments arranged by size:
3678 3440 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5078 6288 5078
Size: 1210 5976
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7186
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2631 2816 3377
Size: 107 122 71 631 605 959 185 561
Cuts at: 3377 5797 136
Size: 2420 1525
Fragments arranged by size:
2420 1525 959 631 605 561 185 122
107 71
BglII A'GATC_T
Cuts at: 2577 7181 2577
Size: 4604 2582
BlpI GC'TnA_GC
Cuts at: 2357 4370 4603 2357
Size: 2013 233 4940
Fragments arranged by size:
4940 2013 233
Bme1580I G_kGCm'C
Cuts at: 1790 3373 3453 5247 6403 1790
Size: 1583 80 1794 1156 2573
Fragments arranged by size:
2573 1794 1583 1156 80
BmgBI CAC'GTC
Cuts at: 1318 3508 1318
Size: 2190 4996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4917 5875 442
Size: 416 53 83 399 24 3500 958 1753
Fragments arranged by size:
3500 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2573 3596 4463 5846 1239
Size: 1334 1023 867 1383 2579
Fragments arranged by size:
2579 1383 1334 1023 867
Bpu10I CC'TnA_GC
Cuts at: 2934 3080 4466 2934
Size: 146 1386 5654
Fragments arranged by size:
5654 1386 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2095 4304 5311 6176 6474 2095
Size: 2209 1007 865 298 2807
Fragments arranged by size:
2807 2209 1007 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 3848 5849 882
Size: 617 552 1797 2001 2219
Fragments arranged by size:
2219 2001 1797 617 552
BsaAI yAC'GTr
Cuts at: 493 1204 2980 3040 3445 4429 4450 4977 493
Size: 711 1776 60 405 984 21 527 2702
Fragments arranged by size:
2702 1776 984 711 527 405 60 21
BsaBI GATnn'nnATC
Cuts at: 7180 7180
Size: 7186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2437 3056 3915 4092
Size: 53 83 186 936 904 619 859 177
Cuts at: 4092 4993 5375 6010 275
Size: 901 382 635 1451
Fragments arranged by size:
1451 936 904 901 859 635 619 382
186 177 83 53
BsaWI w'CCGG_w
Cuts at: 3072 4158 5619 6017 6291 6438 6849 3072
Size: 1086 1461 398 274 147 411 3409
Fragments arranged by size:
3409 1461 1086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4046 4076 4349 4379 1178
Size: 30 165 30 2643 30 273 30 3985
Fragments arranged by size:
3985 2643 273 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 3296 3704 1186
Size: 1384 726 408 4668
Fragments arranged by size:
4668 1384 726 408
BseYI C'CCAG_C
Cuts at: 2088 2120 2224 2380 2859 2884 3004 3031 3258
Size: 32 104 156 479 25 120 27 227
Cuts at: 3258 3541 3718 4177 6389 6885 2088
Size: 283 177 459 2212 496 2389
Fragments arranged by size:
2389 2212 496 479 459 283 227 177
156 120 104 32 27 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2787 3062 3221 3239 1163
Size: 573 1051 275 159 18 5110
Fragments arranged by size:
5110 1051 573 275 159 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3396 4636 5397 6425 1540
Size: 275 186 1072 323 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1072 1028 761 323 275 186
BsiHKAI G_wGCw'C
Cuts at: 3453 3696 3975 4320 4344 4449 5247 5332 6403
Size: 243 279 345 24 105 798 85 1071
Cuts at: 6403 3453
Size: 4236
Fragments arranged by size:
4236 1071 798 345 279 243 105 85
24
BsmI GAATG_Cn'
Cuts at: 3394 4697 4790 3394
Size: 1303 93 5790
Fragments arranged by size:
5790 1303 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2368 2368
Size: 7186
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2636 3373 3453 3696 3767 3975
Size: 399 297 549 737 80 243 71 208
Cuts at: 3975 4320 4344 4449 4505 5247 5332 6403 1391
Size: 345 24 105 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 737 549 399 345 297
243 208 105 85 80 71 56 24
BspEI T'CCGG_A
Cuts at: 6849 6849
Size: 7186
BspHI T'CATG_A
Cuts at: 5075 6805 5075
Size: 1730 5456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4134 4541 844
Size: 3290 407 3489
Fragments arranged by size:
3489 3290 407
BsrBI CCG'CTC
Cuts at: 1394 1823 5073 1394
Size: 429 3250 3507
Fragments arranged by size:
3507 3250 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3897 5681 5855 65
Size: 1183 2649 1784 174 1396
Fragments arranged by size:
2649 1784 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2772 3139 3416 3512 5830 6017 1700
Size: 110 962 367 277 96 2318 187 2869
Fragments arranged by size:
2869 2318 962 367 277 187 110 96
BsrGI T'GTAC_A
Cuts at: 96 4062 96
Size: 3966 3220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7186
BssSI C'ACGA_G
Cuts at: 2037 2318 5246 6258 2037
Size: 281 2928 1012 2965
Fragments arranged by size:
2965 2928 1012 281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4348 794
Size: 3554 3632
BstBI TT'CG_AA
Cuts at: 3462 5990 3462
Size: 2528 4658
BstEII G'GTnAC_C
Cuts at: 1184 3151 1184
Size: 1967 5219
BstXI CCAn_nnnn'nTGG
Cuts at: 3266 4117 3266
Size: 851 6335
BstYI r'GATC_y
Cuts at: 1972 2577 3075 4516 4883 5268 5285 6737 6823
Size: 605 498 1441 367 385 17 1452 86
Cuts at: 6823 6835 7181 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1441 605 498 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3442 3442
Size: 7186
Bsu36I CC'TnA_GG
Cuts at: 2812 2812
Size: 7186
BtgI C'CryG_G
Cuts at: 513 1065 2902 3228 3265 3427 3789 3858 4050
Size: 552 1837 326 37 162 362 69 192
Cuts at: 4050 4352 513
Size: 302 3347
Fragments arranged by size:
3347 1837 552 362 326 302 192 162
69 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2602 2914 487
Size: 683 56 1376 312 4759
Fragments arranged by size:
4759 1376 683 312 56
BtsI GCAGTG_nn'
Cuts at: 2140 2727 2776 3771 4367 4702 5496 5516 2140
Size: 587 49 995 596 335 794 20 3810
Fragments arranged by size:
3810 995 794 596 587 335 49 20
ClaI AT'CG_AT
Cuts at: 1554 2640 2712 1554
Size: 1086 72 6028
Fragments arranged by size:
6028 1086 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7151
DraI TTT'AAA
Cuts at: 4490 4490
Size: 7186
DraIII CAC_nnn'GTG
Cuts at: 3508 3508
Size: 7186
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3959 6193 809
Size: 507 2643 2234 1802
Fragments arranged by size:
2643 2234 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3351 3393 3510
Size: 54 1425 50 275 18 1521 42 117
Cuts at: 3510 3882 4419 4633 5522 7151 8
Size: 372 537 214 889 1629 43
Fragments arranged by size:
1629 1521 1425 889 537 372 275 214
117 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3393 4633 1537
Size: 275 1581 1240 4090
Fragments arranged by size:
4090 1581 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5116 1353
Size: 3763 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2582 5773 6147 6293 387
Size: 1064 138 993 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 993 374 146 138
EcoICRI GAG'CTC
Cuts at: 3694 4318 3694
Size: 624 6562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2573 3596 3739 4285 4301 4409 4463
Size: 393 941 1023 143 546 16 108 54
Cuts at: 4463 5249 5846 6633 6901 1239
Size: 786 597 787 268 1524
Fragments arranged by size:
1524 1023 941 787 786 597 546 393
268 143 108 54 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 2552 3473 3563 4130 2321
Size: 231 921 90 567 5377
Fragments arranged by size:
5377 921 567 231 90
EcoO109I rG'GnC_Cy
Cuts at: 2113 2314 2748 2907 2986 3369 3370 3555 3934
Size: 201 434 159 79 383 1 185 379
Cuts at: 3934 4009 2113
Size: 75 5290
Fragments arranged by size:
5290 434 383 379 201 185 159 79
75 1
EcoRI G'AATT_C
Cuts at: 4495 4873 4495
Size: 378 6808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3358 4111 3358
Size: 753 6433
FspI TGC'GCA
Cuts at: 5692 5692
Size: 7186
HaeII r_GCGC'y
Cuts at: 1678 2406 2440 2795 3116 3685 3838 4143 6333
Size: 728 34 355 321 569 153 305 2190
Cuts at: 6333 1678
Size: 2531
Fragments arranged by size:
2531 2190 728 569 355 321 305 153
34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2904 2936 5827 5859 5901
Size: 32 135 32 1332 32 2891 32 42
Cuts at: 5901 5933 1373
Size: 32 2626
Fragments arranged by size:
2891 2626 1332 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3964 4530 4776 669
Size: 988 281 2026 566 246 3079
Fragments arranged by size:
3079 2026 988 566 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7186
HpaI GTT'AAC
Cuts at: 4776 4776
Size: 7186
KasI G'GCGC_C
Cuts at: 2436 2436
Size: 7186
KpnI G_GTAC'C
Cuts at: 2788 4511 2788
Size: 1723 5463
MfeI C'AATT_G
Cuts at: 4785 4785
Size: 7186
MluI A'CGCG_T
Cuts at: 7062 7062
Size: 7186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 3792 4169 4185 6300 6484 1305
Size: 1086 1401 377 16 2115 184 2007
Fragments arranged by size:
2115 2007 1401 1086 377 184 16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3353 3884 10
Size: 54 1425 343 1521 531 3312
Fragments arranged by size:
3312 1521 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2273 2663 3704 3902 4415 5144 5503
Size: 648 1107 390 1041 198 513 729 359
Cuts at: 5503 5662 518
Size: 159 2042
Fragments arranged by size:
2042 1107 1041 729 648 513 390 359
198 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2380 2447 3046 3574 3664 4602 5279
Size: 514 260 67 599 528 90 938 677
Cuts at: 5279 6427 6672 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 938 677 599 528 514 260
245 90 67
NaeI GCC'GGC
Cuts at: 2774 3141 3514 2774
Size: 367 373 6446
Fragments arranged by size:
6446 373 367
NarI GG'CG_CC
Cuts at: 2437 2437
Size: 7186
NcoI C'CATG_G
Cuts at: 513 1065 3228 3427 3789 3858 513
Size: 552 2163 199 362 69 3841
Fragments arranged by size:
3841 2163 552 362 199 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7186
NgoMIV G'CCGG_C
Cuts at: 2772 3139 3512 2772
Size: 367 373 6446
Fragments arranged by size:
6446 373 367
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7186
NotI GC'GGCC_GC
Cuts at: 4633 4633
Size: 7186
NspI r_CATG'y
Cuts at: 1132 1229 4351 4544 6089 1132
Size: 97 3122 193 1545 2229
Fragments arranged by size:
3122 2229 1545 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7186
PciI A'CATG_T
Cuts at: 6085 6085
Size: 7186
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4197 1108
Size: 3089 4097
PmeI GTTT'AAAC
Cuts at: 4490 4490
Size: 7186
PmlI CAC'GTG
Cuts at: 3040 4450 3040
Size: 1410 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5255 5287 6768 6800 5255
Size: 32 1481 32 5641
Fragments arranged by size:
5641 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2748 2907 2986 3555 3934 2748
Size: 159 79 569 379 6000
Fragments arranged by size:
6000 569 379 159 79
PshAI GACnn'nnGTC
Cuts at: 1613 4112 1613
Size: 2499 4687
PsiI TTA'TAA
Cuts at: 4756 4756
Size: 7186
PspOMI G'GGCC_C
Cuts at: 3369 3369
Size: 7186
PstI C_TGCA'G
Cuts at: 830 2147 3663 4106 4131 4538 830
Size: 1317 1516 443 25 407 3478
Fragments arranged by size:
3478 1516 1317 443 407 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7186
PvuII CAG'CTG
Cuts at: 2380 3574 3664 2380
Size: 1194 90 5902
Fragments arranged by size:
5902 1194 90
RsrII CG'GwC_CG
Cuts at: 3414 3414
Size: 7186
SacI G_AGCT'C
Cuts at: 3696 4320 3696
Size: 624 6562
SalI G'TCGA_C
Cuts at: 1936 4528 1936
Size: 2592 4594
SanDI GG'GwC_CC
Cuts at: 2907 2907
Size: 7186
SbfI CC_TGCA'GG
Cuts at: 4538 4538
Size: 7186
ScaI AGT'ACT
Cuts at: 5434 7146 5434
Size: 1712 5474
SexAI A'CCwGG_T
Cuts at: 2457 2988 2457
Size: 531 6655
SfcI C'TryA_G
Cuts at: 826 1045 2143 2589 3659 4102 4127 4534 5669
Size: 219 1098 446 1070 443 25 407 1135
Cuts at: 5669 6350 6541 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1098 1070 681 446 443 407
219 191 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 2816 2816
Size: 7186
SfoI GGC'GCC
Cuts at: 2438 2438
Size: 7186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7186
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7186
SmaI CCC'GGG
Cuts at: 1872 4513 1872
Size: 2641 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2074 4319 5290 6191 6453 820
Size: 197 926 131 2245 971 901 262 1553
Fragments arranged by size:
2245 1553 971 926 901 262 197 131
SnaBI TAC'GTA
Cuts at: 493 4977 493
Size: 4484 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7186
SphI G_CATG'C
Cuts at: 1132 4351 4544 1132
Size: 3219 193 3774
Fragments arranged by size:
3774 3219 193
SspI AAT'ATT
Cuts at: 5 52 5110 5
Size: 47 5058 2081
Fragments arranged by size:
5058 2081 47
StuI AGG'CCT
Cuts at: 1517 2417 3468 3644 1517
Size: 900 1051 176 5059
Fragments arranged by size:
5059 1051 900 176
StyI C'CwwG_G
Cuts at: 513 1065 2109 3210 3228 3327 3427 3474 3564
Size: 552 1044 1101 18 99 100 47 90
Cuts at: 3564 3789 3858 4625 513
Size: 225 69 767 3074
Fragments arranged by size:
3074 1101 1044 767 552 225 100 99
90 69 47 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2267 2876 3272 5213 5230 5383 5568
Size: 1255 687 609 396 1941 17 153 185
Cuts at: 5568 6013 325
Size: 445 1498
Fragments arranged by size:
1941 1498 1255 687 609 445 396 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4062 4071 5432
Size: 274 80 33 51 1443 2085 9 1361
Cuts at: 5432 7144 96
Size: 1712 138
Fragments arranged by size:
2085 1712 1443 1361 274 138 80 51
33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4349 4381 7146 7178 4349
Size: 32 2765 32 4357
Fragments arranged by size:
4357 2765 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7186
XbaI T'CTAG_A
Cuts at: 4522 4522
Size: 7186
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2096 3266 3361 3571 4359 2096
Size: 1170 95 210 788 4923
Fragments arranged by size:
4923 1170 788 210 95
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7186
XmaI C'CCGG_G
Cuts at: 1870 4511 1870
Size: 2641 4545
XmnI GAAnn'nnTTC
Cuts at: 5315 5315
Size: 7186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4994 276
Size: 53 83 186 936 3460 2468
Fragments arranged by size:
3460 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI EcoRV
FseI FspAI NruI NsiI PacI PfoI PspXI PsrI
SacII SapI SrfI SwaI