Restriction map for
pFN21AB6016
(Circular) (Six-base) MAPSORT of: pFN21AB6016.seq Check: 6280 from: 1 to: 7528
pFN21AB6016 7528 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5338 278
Size: 53 83 186 936 3802 2468
Fragments arranged by size:
3802 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4871 1937
Size: 2934 4594
Acc65I G'GTAC_C
Cuts at: 2877 4849 2877
Size: 1972 5556
AclI AA'CG_TT
Cuts at: 1563 2554 5655 6028 1563
Size: 991 3101 373 3063
Fragments arranged by size:
3101 3063 991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2853 4059 4416 4547 5591 6975 7243 1632
Size: 1221 1206 357 131 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1221 1206 1044 357 268 131
AflII C'TTAA_G
Cuts at: 820 1017 4790 820
Size: 197 3773 3558
Fragments arranged by size:
3773 3558 197
AflIII A'CryG_T
Cuts at: 1203 4247 6427 7404 1203
Size: 3044 2180 977 1327
Fragments arranged by size:
3044 2180 1327 977
AgeI A'CCGG_T
Cuts at: 6359 6359
Size: 7528
AhdI GACnn_n'nnGTC
Cuts at: 6257 6257
Size: 7528
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7528
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3801 1184
Size: 2617 4911
AlwNI CAG_nnn'CTG
Cuts at: 2045 3707 4317 5271 6843 2045
Size: 1662 610 954 1572 2730
Fragments arranged by size:
2730 1662 1572 954 610
ApaLI G'TGCA_C
Cuts at: 4009 5585 6741 4009
Size: 1576 1156 4796
Fragments arranged by size:
4796 1576 1156
ApoI r'AATT_y
Cuts at: 1493 2690 2932 3188 3366 3465 3671 4155 4585
Size: 1197 242 256 178 99 206 484 430
Cuts at: 4585 4837 5063 5215 7324 1493
Size: 252 226 152 2109 1697
Fragments arranged by size:
2109 1697 1197 484 430 256 252 242
226 206 178 152 99
AseI AT'TA_AT
Cuts at: 160 1032 6082 160
Size: 872 5050 1606
Fragments arranged by size:
5050 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7528
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 4004 4853 1870
Size: 73 445 1616 849 4545
Fragments arranged by size:
4545 1616 849 445 73
BamHI G'GATC_C
Cuts at: 3060 4306 4858 5225 3060
Size: 1246 552 367 5363
Fragments arranged by size:
5363 1246 552 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2877 4669 4849 6304
Size: 325 217 627 308 782 1792 180 1455
Cuts at: 6304 618
Size: 1842
Fragments arranged by size:
1842 1792 1455 782 627 325 308 217
180
BanII G_rGCy'C
Cuts at: 1391 3594 3656 4847 1391
Size: 2203 62 1191 4072
Fragments arranged by size:
4072 2203 1191 62
BbeI G_GCGC'C
Cuts at: 2099 2099
Size: 7528
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2386 3999 928
Size: 971 487 1613 4457
Fragments arranged by size:
4457 1613 971 487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2049 2083 3995 4029 4334 4368 5719
Size: 34 112 34 1912 34 305 34 1351
Cuts at: 5719 5753 1903
Size: 34 3678
Fragments arranged by size:
3678 1912 1351 305 112 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4373 5420 6630 4373
Size: 1047 1210 5271
Fragments arranged by size:
5271 1210 1047
BclI T'GATC_A
Cuts at: 1548 2679 1548
Size: 1131 6397
BfrBI ATG'CAT
Cuts at: 2428 4485 2428
Size: 2057 5471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2057 6139
Size: 107 122 71 631 605 343 42 4082
Cuts at: 6139 136
Size: 1525
Fragments arranged by size:
4082 1525 631 605 343 122 107 71
42
BglII A'GATC_T
Cuts at: 3178 7523 3178
Size: 4345 3183
BlpI GC'TnA_GC
Cuts at: 4945 4945
Size: 7528
Bme1580I G_kGCm'C
Cuts at: 1790 4013 4674 5589 6745 1790
Size: 2223 661 915 1156 2573
Fragments arranged by size:
2573 2223 1156 915 661
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 6700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5259 6217 442
Size: 416 53 83 399 24 3842 958 1753
Fragments arranged by size:
3842 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2067 2477 4286 6188 1239
Size: 828 410 1809 1902 2579
Fragments arranged by size:
2579 1902 1809 828 410
Bpu10I CC'TnA_GC
Cuts at: 3140 3140
Size: 7528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3670 4429 4540 5653 6518 6816 3670
Size: 759 111 1113 865 298 4382
Fragments arranged by size:
4382 1113 865 759 298 111
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4684 6191 882
Size: 617 3185 1507 2219
Fragments arranged by size:
3185 2219 1507 617
BsaAI yAC'GTr
Cuts at: 493 1204 4248 5319 493
Size: 711 3044 1071 2702
Fragments arranged by size:
3044 2702 1071 711
BsaBI GATnn'nnATC
Cuts at: 3729 7522 3729
Size: 3793 3735
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 5335 5717 6352
Size: 53 83 186 936 563 3239 382 635
Cuts at: 6352 275
Size: 1451
Fragments arranged by size:
3239 1451 936 635 563 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3998 5961 6359 6633 6780 7191 3998
Size: 1963 398 274 147 411 4335
Fragments arranged by size:
4335 1963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2502 2532 2982 3012 1178
Size: 30 165 30 1099 30 450 30 5694
Fragments arranged by size:
5694 1099 450 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3446 1186
Size: 2260 5268
BseYI C'CCAG_C
Cuts at: 4310 6731 7227 4310
Size: 2421 496 4611
Fragments arranged by size:
4611 2421 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2280 3098 3999 1163
Size: 573 544 818 901 4692
Fragments arranged by size:
4692 901 818 573 544
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2086 4978 5739 6767 1540
Size: 275 186 85 2892 761 1028 2301
Fragments arranged by size:
2892 2301 1028 761 275 186 85
BsiHKAI G_wGCw'C
Cuts at: 3075 3594 3656 4013 4193 5589 5674 6745 3075
Size: 519 62 357 180 1396 85 1071 3858
Fragments arranged by size:
3858 1396 1071 519 357 180 85 62
BsiWI C'GTAC_G
Cuts at: 2139 2139
Size: 7528
BsmI GAATG_Cn'
Cuts at: 5039 5132 5039
Size: 93 7435
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3075 3594 3656 4013 4193 4674 4847
Size: 399 1285 519 62 357 180 481 173
Cuts at: 4847 5589 5674 6745 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1285 1071 742 519 481 399 357
180 173 85 62
BspEI T'CCGG_A
Cuts at: 3998 7191 3998
Size: 3193 4335
BspHI T'CATG_A
Cuts at: 2157 2646 5417 7147 2157
Size: 489 2771 1730 2538
Fragments arranged by size:
2771 2538 1730 489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4883 844
Size: 4039 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2015 5415 1394
Size: 429 192 3400 3507
Fragments arranged by size:
3507 3400 429 192
BsrDI GCAATG_nn'
Cuts at: 65 1248 6023 6197 65
Size: 1183 4775 174 1396
Fragments arranged by size:
4775 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 4693 6172 6359 1700
Size: 110 320 2563 1479 187 2869
Fragments arranged by size:
2869 2563 1479 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7528
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7528
BssSI C'ACGA_G
Cuts at: 5588 6600 5588
Size: 1012 6516
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4278 794
Size: 3484 4044
BstBI TT'CG_AA
Cuts at: 6332 6332
Size: 7528
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7528
BstYI r'GATC_y
Cuts at: 1972 3060 3178 4306 4399 4462 4785 4858 5225
Size: 1088 118 1128 93 63 323 73 367
Cuts at: 5225 5610 5627 7079 7165 7177 7523 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1128 1088 385 367 346 323
118 93 86 73 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 4458 4458
Size: 7528
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2055 2191 3632 4321 513
Size: 552 939 27 24 136 1441 689 3720
Fragments arranged by size:
3720 1441 939 689 552 136 27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6789
Fragments arranged by size:
6789 683 56
BtsI GCAGTG_nn'
Cuts at: 3305 4011 4288 5044 5838 5858 3305
Size: 706 277 756 794 20 4975
Fragments arranged by size:
4975 794 756 706 277 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7493
DraI TTT'AAA
Cuts at: 3321 4832 3321
Size: 1511 6017
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6535 809
Size: 507 5219 1802
Fragments arranged by size:
5219 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2028 2083
Size: 54 1425 50 275 18 177 21 55
Cuts at: 2083 2768 4807 4975 5864 7493 8
Size: 685 2039 168 889 1629 43
Fragments arranged by size:
2039 1629 1425 889 685 275 177 168
55 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2083 4975 1537
Size: 275 271 2892 4090
Fragments arranged by size:
4090 2892 275 271
EarI CTCTTCn'nnn_
Cuts at: 1353 3633 3819 3861 4035 5458 1353
Size: 2280 186 42 174 1423 3423
Fragments arranged by size:
3423 2280 1423 186 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2091 6115 6489 6635 387
Size: 1064 138 502 4024 374 146 1280
Fragments arranged by size:
4024 1280 1064 502 374 146 138
EcoICRI GAG'CTC
Cuts at: 3592 3654 3592
Size: 62 7466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2477 2853 4059 4286 4416 4547
Size: 393 435 410 376 1206 227 130 131
Cuts at: 4547 5591 6188 6975 7243 1239
Size: 1044 597 787 268 1524
Fragments arranged by size:
1524 1206 1044 787 597 435 410 393
376 268 227 131 130
EcoNI CCTnn'n_nnAGG
Cuts at: 3290 3290
Size: 7528
EcoO109I rG'GnC_Cy
Cuts at: 2725 3607 2725
Size: 882 6646
EcoRI G'AATT_C
Cuts at: 3465 4585 4837 5215 3465
Size: 1120 252 378 5778
Fragments arranged by size:
5778 1120 378 252
EcoRV GAT'ATC
Cuts at: 2173 2453 2173
Size: 280 7248
FspI TGC'GCA
Cuts at: 6034 6034
Size: 7528
HaeII r_GCGC'y
Cuts at: 1678 2099 6675 1678
Size: 421 4576 2531
Fragments arranged by size:
4576 2531 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2460 2492 2502 2534 4371
Size: 32 135 32 888 32 10 32 1837
Cuts at: 4371 4384 4403 4416 6169 6201 6243 6275 1373
Size: 13 19 13 1753 32 42 32 2626
Fragments arranged by size:
2626 1837 1753 888 135 42 32 32
32 32 32 32 19 13 13 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2230 4872 5118 669
Size: 988 281 292 2642 246 3079
Fragments arranged by size:
3079 2642 988 292 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7528
HpaI GTT'AAC
Cuts at: 5118 5118
Size: 7528
KasI G'GCGC_C
Cuts at: 2095 2095
Size: 7528
KpnI G_GTAC'C
Cuts at: 2881 4853 2881
Size: 1972 5556
MfeI C'AATT_G
Cuts at: 5127 5127
Size: 7528
MluI A'CGCG_T
Cuts at: 7404 7404
Size: 7528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 3712 4110 6642 6826 1305
Size: 880 1527 398 2532 184 2007
Fragments arranged by size:
2532 2007 1527 880 398 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2770 4809 10
Size: 54 1425 343 938 2039 2729
Fragments arranged by size:
2729 2039 1425 938 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2310 2591 2631 3131 5486 5845 6004
Size: 648 1144 281 40 500 2355 359 159
Cuts at: 6004 518
Size: 2042
Fragments arranged by size:
2355 2042 1144 648 500 359 281 159
40
MspA1I CmG'CkG
Cuts at: 1606 2033 2045 2057 2199 2440 4317 4551 4944
Size: 427 12 12 142 241 1877 234 393
Cuts at: 4944 5621 6769 7014 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1877 1148 677 427 393 245 241
234 142 12 12
NaeI GCC'GGC
Cuts at: 2132 2132
Size: 7528
NarI GG'CG_CC
Cuts at: 2096 2096
Size: 7528
NcoI C'CATG_G
Cuts at: 513 1065 2004 3632 4321 513
Size: 552 939 1628 689 3720
Fragments arranged by size:
3720 1628 939 689 552
NdeI CA'TA_TG
Cuts at: 387 3209 387
Size: 2822 4706
NgoMIV G'CCGG_C
Cuts at: 2130 2130
Size: 7528
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7528
NotI GC'GGCC_GC
Cuts at: 4975 4975
Size: 7528
NsiI A_TGCA'T
Cuts at: 2430 4487 2430
Size: 2057 5471
NspI r_CATG'y
Cuts at: 1132 1229 3136 3359 4219 4886 6431 1132
Size: 97 1907 223 860 667 1545 2229
Fragments arranged by size:
2229 1907 1545 860 667 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7528
PciI A'CATG_T
Cuts at: 6427 6427
Size: 7528
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3213 3920 1108
Size: 2105 707 4716
Fragments arranged by size:
4716 2105 707
PfoI T'CCnGG_A
Cuts at: 4302 4421 4302
Size: 119 7409
PmeI GTTT'AAAC
Cuts at: 4832 4832
Size: 7528
PmlI CAC'GTG
Cuts at: 4248 4248
Size: 7528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5597 5629 7110 7142 5597
Size: 32 1481 32 5983
Fragments arranged by size:
5983 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 3607 2725
Size: 882 6646
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7528
PsiI TTA'TAA
Cuts at: 2420 5098 2420
Size: 2678 4850
PstI C_TGCA'G
Cuts at: 830 4283 4880 830
Size: 3453 597 3478
Fragments arranged by size:
3478 3453 597
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7528
PvuII CAG'CTG
Cuts at: 2045 2199 2440 4317 2045
Size: 154 241 1877 5256
Fragments arranged by size:
5256 1877 241 154
SacI G_AGCT'C
Cuts at: 3594 3656 3594
Size: 62 7466
SacII CC_GC'GG
Cuts at: 2034 2058 2034
Size: 24 7504
SalI G'TCGA_C
Cuts at: 1936 4870 1936
Size: 2934 4594
SbfI CC_TGCA'GG
Cuts at: 4880 4880
Size: 7528
ScaI AGT'ACT
Cuts at: 5776 7488 5776
Size: 1712 5816
SfcI C'TryA_G
Cuts at: 826 1045 3051 4279 4876 6011 6692 6883 826
Size: 219 2006 1228 597 1135 681 191 1471
Fragments arranged by size:
2006 1471 1228 1135 681 597 219 191
SfoI GGC'GCC
Cuts at: 2097 2097
Size: 7528
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7528
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7528
SmaI CCC'GGG
Cuts at: 1872 4855 1872
Size: 2983 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2388 3649 4408 4555 4790 5632
Size: 197 926 445 1261 759 147 235 842
Cuts at: 5632 6533 6795 820
Size: 901 262 1553
Fragments arranged by size:
1553 1261 926 901 842 759 445 262
235 197 147
SnaBI TAC'GTA
Cuts at: 493 5319 493
Size: 4826 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7528
SphI G_CATG'C
Cuts at: 1132 3136 4219 4886 1132
Size: 2004 1083 667 3774
Fragments arranged by size:
3774 2004 1083 667
SspI AAT'ATT
Cuts at: 5 52 2810 5452 5
Size: 47 2758 2642 2081
Fragments arranged by size:
2758 2642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7528
StyI C'CwwG_G
Cuts at: 513 1065 2004 3632 4321 4967 513
Size: 552 939 1628 689 646 3074
Fragments arranged by size:
3074 1628 939 689 646 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2743 5555 5572 5725 5910 6355 325
Size: 1255 1163 2812 17 153 185 445 1498
Fragments arranged by size:
2812 1498 1255 1163 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 3289 5774
Size: 274 80 33 51 1443 141 1171 2485
Cuts at: 5774 7486 96
Size: 1712 138
Fragments arranged by size:
2485 1712 1443 1171 274 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2982 3014 7488 7520 2982
Size: 32 4474 32 2990
Fragments arranged by size:
4474 2990 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7528
XbaI T'CTAG_A
Cuts at: 4145 4864 4145
Size: 719 6809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3232 4619 3232
Size: 1387 6141
XhoI C'TCGA_G
Cuts at: 1943 2388 1943
Size: 445 7083
XmaI C'CCGG_G
Cuts at: 1870 4853 1870
Size: 2983 4545
XmnI GAAnn'nnTTC
Cuts at: 2153 3568 4206 5657 2153
Size: 1415 638 1451 4024
Fragments arranged by size:
4024 1451 1415 638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5336 276
Size: 53 83 186 936 3802 2468
Fragments arranged by size:
3802 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI
NruI PacI PspOMI PspXI PsrI RsrII SanDI SapI
SexAI SfiI SrfI SwaI