Restriction map for
pFN21AB6006
(Circular) (Six-base) MAPSORT of: pFN21AB6006.seq Check: 8768 from: 1 to: 12109
pFN21AB6006 12109 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2166 9244 2166
Size: 7078 5031
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9919 278
Size: 53 83 186 936 8383 2468
Fragments arranged by size:
8383 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4764 5151 5369 5414 6994 9452 1937
Size: 2827 387 218 45 1580 2458 4594
Fragments arranged by size:
4594 2827 2458 1580 387 218 45
Acc65I G'GTAC_C
Cuts at: 3332 9300 9430 3332
Size: 5968 130 6011
Fragments arranged by size:
6011 5968 130
AclI AA'CG_TT
Cuts at: 1563 10236 10609 1563
Size: 8673 373 3063
Fragments arranged by size:
8673 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 2598 2789 3390 3557 3659 3789 3963
Size: 669 297 191 601 167 102 130 174
Cuts at: 3963 4266 4560 4625 4722 4797 5073 5103 5148
Size: 303 294 65 97 75 276 30 45
Cuts at: 5148 5586 5595 5712 6114 6591 6973 7080 7824
Size: 438 9 117 402 477 382 107 744
Cuts at: 7824 7875 8085 8172 8373 8523 8565 10172 11556
Size: 51 210 87 201 150 42 1607 1384
Cuts at: 11556 11824 1632
Size: 268 1917
Fragments arranged by size:
1917 1607 1384 744 669 601 477 438
402 382 303 297 294 276 268 210
201 191 174 167 150 130 117 107
102 97 87 75 65 51 45 42
30 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11912
AflIII A'CryG_T
Cuts at: 1203 4885 6627 11008 11985 1203
Size: 3682 1742 4381 977 1327
Fragments arranged by size:
4381 3682 1742 1327 977
AgeI A'CCGG_T
Cuts at: 10940 10940
Size: 12109
AhdI GACnn_n'nnGTC
Cuts at: 6166 7672 10838 6166
Size: 1506 3166 7437
Fragments arranged by size:
7437 3166 1506
AleI CACnn'nnGTG
Cuts at: 1166 2313 2372 3587 3713 4890 1166
Size: 1147 59 1215 126 1177 8385
Fragments arranged by size:
8385 1215 1177 1147 126 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2390 3320 3689 6993 9266 1184
Size: 1206 930 369 3304 2273 4027
Fragments arranged by size:
4027 3304 2273 1206 930 369
AlwNI CAG_nnn'CTG
Cuts at: 3547 3812 4385 4424 4820 4847 5168 6341 6782
Size: 265 573 39 396 27 321 1173 441
Cuts at: 6782 8695 9852 11424 3547
Size: 1913 1157 1572 4232
Fragments arranged by size:
4232 1913 1572 1173 1157 573 441 396
321 265 39 27
ApaLI G'TGCA_C
Cuts at: 10166 11322 10166
Size: 1156 10953
ApoI r'AATT_y
Cuts at: 1493 3353 4778 5339 5477 5654 6095 6947 9128
Size: 1860 1425 561 138 177 441 852 2181
Cuts at: 9128 9418 9644 9796 11905 1493
Size: 290 226 152 2109 1697
Fragments arranged by size:
2181 2109 1860 1697 1425 852 561 441
290 226 177 152 138
AseI AT'TA_AT
Cuts at: 160 1032 8310 10663 160
Size: 872 7278 2353 1606
Fragments arranged by size:
7278 2353 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12109
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 5421 9434 1870
Size: 73 1053 2425 4013 4545
Fragments arranged by size:
4545 4013 2425 1053 73
BamHI G'GATC_C
Cuts at: 6218 9439 9806 6218
Size: 3221 367 8521
Fragments arranged by size:
8521 3221 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3332 3577 5174 6275 9300
Size: 325 217 627 1545 245 1597 1101 3025
Cuts at: 9300 9430 10885 618
Size: 130 1455 1842
Fragments arranged by size:
3025 1842 1597 1545 1455 1101 627 325
245 217 130
BanII G_rGCy'C
Cuts at: 1391 2187 2454 2997 3020 4746 5227 5394 5774
Size: 796 267 543 23 1726 481 167 380
Cuts at: 5774 6153 6686 7164 7491 8392 9195 9428 1391
Size: 379 533 478 327 901 803 233 4072
Fragments arranged by size:
4072 1726 901 803 796 543 533 481
478 380 379 327 267 233 167 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3917 3951 4596 5016 5136 5331 5655
Size: 971 2018 34 645 420 120 195 324
Cuts at: 5655 6420 6555 6630 7598 7691 7844 8553 8735
Size: 765 135 75 968 93 153 709 182
Cuts at: 8735 9141 928
Size: 406 3896
Fragments arranged by size:
3896 2018 971 968 765 709 645 420
406 324 195 182 153 135 120 93
75 34
BbvCI CC'TCA_GC
Cuts at: 4572 4857 6732 9186 4572
Size: 285 1875 2454 7495
Fragments arranged by size:
7495 2454 1875 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5141 5175 8976 9010 10300 10334 1903
Size: 34 3204 34 3801 34 1290 34 3678
Fragments arranged by size:
3801 3678 3204 1290 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10001 11211 10001
Size: 1210 10899
BclI T'GATC_A
Cuts at: 1548 2340 2385 1548
Size: 792 45 11272
Fragments arranged by size:
11272 792 45
BfrBI ATG'CAT
Cuts at: 4955 4955
Size: 12109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2063 3863 4382
Size: 107 122 71 631 605 391 1800 519
Cuts at: 4382 6674 10720 136
Size: 2292 4046 1525
Fragments arranged by size:
4046 2292 1800 1525 631 605 519 391
122 107 71
BglII A'GATC_T
Cuts at: 3382 5663 6920 8716 9328 12104 3382
Size: 2281 1257 1796 612 2776 3387
Fragments arranged by size:
3387 2776 2281 1796 1257 612
BlpI GC'TnA_GC
Cuts at: 9526 9526
Size: 12109
Bme1580I G_kGCm'C
Cuts at: 1790 3582 8513 9057 10170 11326 1790
Size: 1792 4931 544 1113 1156 2573
Fragments arranged by size:
4931 2573 1792 1156 1113 544
BmgBI CAC'GTC
Cuts at: 1318 5428 1318
Size: 4110 7999
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3481 5221 9840
Size: 416 53 83 399 24 2064 1740 4619
Cuts at: 9840 10798 442
Size: 958 1753
Fragments arranged by size:
4619 2064 1753 1740 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 5805 6757 7183 7352 7354 8530 2719
Size: 3086 952 426 169 2 1176 6298
Fragments arranged by size:
6298 3086 1176 952 426 169 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 3143 3831 4428 4598 4760 4842 5298
Size: 1205 699 688 597 170 162 82 456
Cuts at: 5298 6303 6987 7078 7592 8385 8592 10769 1239
Size: 1005 684 91 514 793 207 2177 2579
Fragments arranged by size:
2579 2177 1205 1005 793 699 688 684
597 514 456 207 170 162 91 82
Bpu10I CC'TnA_GC
Cuts at: 4572 4857 6732 6966 9186 4572
Size: 285 1875 234 2220 7495
Fragments arranged by size:
7495 2220 1875 285 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3284 8345 10234 11099 11397 3284
Size: 5061 1889 865 298 3996
Fragments arranged by size:
5061 3996 1889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3788 5435 7769 8954 9302 10772 882
Size: 617 2289 1647 2334 1185 348 1470 2219
Fragments arranged by size:
2334 2289 2219 1647 1470 1185 617 348
BsaAI yAC'GTr
Cuts at: 493 1204 3724 4888 5675 6630 8586 8939 9160
Size: 711 2520 1164 787 955 1956 353 221
Cuts at: 9160 9900 493
Size: 740 2702
Fragments arranged by size:
2702 2520 1956 1164 955 787 740 711
353 221
BsaBI GATnn'nnATC
Cuts at: 7149 12103 7149
Size: 4954 7155
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 5849 9916 10298
Size: 53 83 186 936 536 3780 4067 382
Cuts at: 10298 10933 275
Size: 635 1451
Fragments arranged by size:
4067 3780 1451 936 635 536 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2444 10542 10940 11214 11361 11772 2444
Size: 8098 398 274 147 411 2781
Fragments arranged by size:
8098 2781 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2604 2634 3038 3068 7115
Size: 30 165 30 1201 30 404 30 4047
Cuts at: 7115 7145 7732 7762 8902 8932 1178
Size: 30 587 30 1140 30 4355
Fragments arranged by size:
4355 4047 1201 1140 587 404 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 3971 4227 4748 4865 4874 5072 5084
Size: 979 1806 256 521 117 9 198 12
Cuts at: 5084 5111 5195 5327 7139 7224 7257 9203 1186
Size: 27 84 132 1812 85 33 1946 4092
Fragments arranged by size:
4092 1946 1812 1806 979 521 256 198
132 117 85 84 33 27 12 9
BseYI C'CCAG_C
Cuts at: 3827 8015 9194 11312 11808 3827
Size: 4188 1179 2118 496 4128
Fragments arranged by size:
4188 4128 2118 1179 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 7044 8919 1163
Size: 573 5308 1875 4353
Fragments arranged by size:
5308 4353 1875 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 8526 9559 10320 11348 1540
Size: 275 186 12 6513 1033 761 1028 2301
Fragments arranged by size:
6513 2301 1033 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2157 2187 2454 2997 6686 7164 10170 10255 11326
Size: 30 267 543 3689 478 3006 85 1071
Cuts at: 11326 2157
Size: 2940
Fragments arranged by size:
3689 3006 2940 1071 543 478 267 85
30
BsmI GAATG_Cn'
Cuts at: 2225 6845 8974 9620 9713 2225
Size: 4620 2129 646 93 4621
Fragments arranged by size:
4621 4620 2129 646 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5418 5450 5418
Size: 32 12077
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2187 2454 2997 3020 3582 4746
Size: 399 367 30 267 543 23 562 1164
Cuts at: 4746 5227 5394 5774 6153 6686 7164 7491 8392
Size: 481 167 380 379 533 478 327 901
Cuts at: 8392 8513 9057 9195 9428 10170 10255 11326 1391
Size: 121 544 138 233 742 85 1071 2174
Fragments arranged by size:
2174 1164 1071 901 742 562 544 543
533 481 478 399 380 379 367 327
267 233 167 138 121 85 30 23
BspEI T'CCGG_A
Cuts at: 11772 11772
Size: 12109
BspHI T'CATG_A
Cuts at: 5260 9274 9998 11728 5260
Size: 4014 724 1730 5641
Fragments arranged by size:
5641 4014 1730 724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 5254 5542 9244 9464 844
Size: 1322 3088 288 3702 220 3489
Fragments arranged by size:
3702 3489 3088 1322 288 220
BsrBI CCG'CTC
Cuts at: 1394 1823 5551 9996 1394
Size: 429 3728 4445 3507
Fragments arranged by size:
4445 3728 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7975 10604 10778 65
Size: 1183 6727 2629 174 1396
Fragments arranged by size:
6727 2629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4796 10753 10940 1700
Size: 110 2986 5957 187 2869
Fragments arranged by size:
5957 2986 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 12109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12109
BssSI C'ACGA_G
Cuts at: 2414 7371 8255 10169 11181 2414
Size: 4957 884 1914 1012 3342
Fragments arranged by size:
4957 3342 1914 1012 884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3443 794
Size: 2649 9460
BstBI TT'CG_AA
Cuts at: 10913 10913
Size: 12109
BstEII G'GTnAC_C
Cuts at: 1184 5905 1184
Size: 4721 7388
BstXI CCAn_nnnn'nTGG
Cuts at: 3589 5234 5401 8006 3589
Size: 1645 167 2605 7692
Fragments arranged by size:
7692 2605 1645 167
BstYI r'GATC_y
Cuts at: 1972 2298 3382 3562 4507 5663 6218 6536 6920
Size: 326 1084 180 945 1156 555 318 384
Cuts at: 6920 8716 9328 9439 9806 10191 10208 11660 11746
Size: 1796 612 111 367 385 17 1452 86
Cuts at: 11746 11758 12104 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1796 1452 1156 1084 945 612 555
385 384 367 346 326 318 180 111
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3702 4503 4731 5334 5901 5943 7629 8160 8922
Size: 801 228 603 567 42 1686 531 762
Cuts at: 8922 3702
Size: 6889
Fragments arranged by size:
6889 1686 801 762 603 567 531 228
42
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 5400 5640 6141 8658 513
Size: 552 939 9 3387 240 501 2517 3964
Fragments arranged by size:
3964 3387 2517 939 552 501 240 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 487
Size: 683 56 812 10558
Fragments arranged by size:
10558 812 683 56
BtsI GCAGTG_nn'
Cuts at: 2435 2838 3329 5343 5998 8202 9009 9625 10419
Size: 403 491 2014 655 2204 807 616 794
Cuts at: 10419 10439 2435
Size: 20 4105
Fragments arranged by size:
4105 2204 2014 807 794 655 616 491
403 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7044 7079 529
Size: 35 6480 35 5559
Fragments arranged by size:
6480 5559 35 35
DraI TTT'AAA
Cuts at: 4269 6544 9413 4269
Size: 2275 2869 6965
Fragments arranged by size:
6965 2869 2275
DraIII CAC_nnn'GTG
Cuts at: 7601 7601
Size: 12109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7939 8579 9040 11116 809
Size: 507 6623 640 461 2076 1802
Fragments arranged by size:
6623 2076 1802 640 507 461
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3549 3571 4983
Size: 54 1425 50 275 18 1719 22 1412
Cuts at: 4983 5236 6421 6952 7996 8523 8679 8997 9556
Size: 253 1185 531 1044 527 156 318 559
Cuts at: 9556 10445 12074 8
Size: 889 1629 43
Fragments arranged by size:
1719 1629 1425 1412 1185 1044 889 559
531 527 318 275 253 156 54 50
43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 8523 9556 1537
Size: 275 6711 1033 4090
Fragments arranged by size:
6711 4090 1033 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3122 3831 4032 4071 4146 4287 4521 4536
Size: 1769 709 201 39 75 141 234 15
Cuts at: 4536 4590 4827 4863 4964 5049 5448 5641 5762
Size: 54 237 36 101 85 399 193 121
Cuts at: 5762 7207 7462 7851 8499 8940 10039 1353
Size: 1445 255 389 648 441 1099 3423
Fragments arranged by size:
3423 1769 1445 1099 709 648 441 399
389 255 237 234 201 193 141 121
101 85 75 54 39 36 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2877 4958 10696 11070 11216 387
Size: 1064 138 1288 2081 5738 374 146 1280
Fragments arranged by size:
5738 2081 1288 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2185 2452 2995 6684 7162 2185
Size: 267 543 3689 478 7132
Fragments arranged by size:
7132 3689 543 478 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2444 2598 2789 3143 3390 3557
Size: 393 669 143 154 191 354 247 167
Cuts at: 3557 3659 3789 3831 3963 4266 4428 4560 4598
Size: 102 130 42 132 303 162 132 38
Cuts at: 4598 4625 4722 4760 4797 4842 5073 5103 5148
Size: 27 97 38 37 45 231 30 45
Cuts at: 5148 5298 5586 5595 5712 6114 6303 6591 6973
Size: 150 288 9 117 402 189 288 382
Cuts at: 6973 6987 7078 7080 7592 7824 7875 8085 8172
Size: 14 91 2 512 232 51 210 87
Cuts at: 8172 8373 8385 8523 8565 8592 10172 10769 11556
Size: 201 12 138 42 27 1580 597 787
Cuts at: 11556 11824 1239
Size: 268 1524
Fragments arranged by size:
1580 1524 787 669 597 512 402 393
382 354 303 288 288 268 247 232
231 210 201 191 189 167 162 154
150 143 138 132 132 130 117 102
97 91 87 51 45 45 42 42
38 38 37 30 27 27 14 12
9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2580 3063 7574 8403 2580
Size: 483 4511 829 6286
Fragments arranged by size:
6286 4511 829 483
EcoO109I rG'GnC_Cy
Cuts at: 4485 4664 7644 8011 4485
Size: 179 2980 367 8583
Fragments arranged by size:
8583 2980 367 179
EcoRI G'AATT_C
Cuts at: 3353 5339 5477 6095 9418 9796 3353
Size: 1986 138 618 3323 378 5666
Fragments arranged by size:
5666 3323 1986 618 378 138
EcoRV GAT'ATC
Cuts at: 5382 7354 5382
Size: 1972 10137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 12109
FspI TGC'GCA
Cuts at: 3677 10615 3677
Size: 6938 5171
HaeII r_GCGC'y
Cuts at: 1678 4902 11256 1678
Size: 3224 6354 2531
Fragments arranged by size:
6354 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2290 2315 2322 2604
Size: 32 135 32 711 7 25 7 282
Cuts at: 2604 2636 2861 2893 5396 5428 5447 5479 5717
Size: 32 225 32 2503 32 19 32 238
Cuts at: 5717 5749 6020 6052 6152 6184 6617 6649 6698
Size: 32 271 32 100 32 433 32 49
Cuts at: 6698 6730 6905 6937 7115 7147 7216 7238 7248
Size: 32 175 32 178 32 69 22 10
Cuts at: 7248 7270 7400 7432 7463 7495 7658 7690 8780
Size: 22 130 32 31 32 163 32 1090
Cuts at: 8780 8812 8840 8872 9256 9288 10750 10782 10824
Size: 32 28 32 384 32 1462 32 42
Cuts at: 10824 10856 1373
Size: 32 2626
Fragments arranged by size:
2626 2503 1462 1090 711 433 384 282
271 238 225 178 175 163 135 130
100 69 49 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 31 28 25 22 22 19 10
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 5152 6995 9453 9699 669
Size: 988 281 3214 1843 2458 246 3079
Fragments arranged by size:
3214 3079 2458 1843 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 12109
HpaI GTT'AAC
Cuts at: 9699 9699
Size: 12109
KpnI G_GTAC'C
Cuts at: 3336 9304 9434 3336
Size: 5968 130 6011
Fragments arranged by size:
6011 5968 130
MfeI C'AATT_G
Cuts at: 9708 9708
Size: 12109
MluI A'CGCG_T
Cuts at: 11985 11985
Size: 12109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2923 3489 8535 9201 11223 11407 1305
Size: 1618 566 5046 666 2022 184 2007
Fragments arranged by size:
5046 2022 2007 1618 666 566 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3551 3573 4985 6423 6954
Size: 54 1425 343 1719 22 1412 1438 531
Cuts at: 6954 7998 8681 10
Size: 1044 683 3438
Fragments arranged by size:
3438 1719 1438 1425 1412 1044 683 531
343 54 22
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 2372 3122 3587 3713 4890 5399
Size: 648 1147 59 750 465 126 1177 509
Cuts at: 5399 6557 8649 9254 10067 10426 10585 518
Size: 1158 2092 605 813 359 159 2042
Fragments arranged by size:
2092 2042 1177 1158 1147 813 750 648
605 509 465 359 159 126 59
MspA1I CmG'CkG
Cuts at: 1606 2980 4262 4397 4424 4439 4576 5738 8828
Size: 1374 1282 135 27 15 137 1162 3090
Cuts at: 8828 9525 10202 11350 11595 1606
Size: 697 677 1148 245 2120
Fragments arranged by size:
3090 2120 1374 1282 1162 1148 697 677
245 137 135 27 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 5400 5640 6141 8658 513
Size: 552 939 3396 240 501 2517 3964
Fragments arranged by size:
3964 3396 2517 939 552 501 240
NdeI CA'TA_TG
Cuts at: 387 6639 7873 387
Size: 6252 1234 4623
Fragments arranged by size:
6252 4623 1234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12109
NotI GC'GGCC_GC
Cuts at: 9556 9556
Size: 12109
NsiI A_TGCA'T
Cuts at: 4957 4957
Size: 12109
NspI r_CATG'y
Cuts at: 1132 1229 2974 6731 7087 9253 9396 9467 11012
Size: 97 1745 3757 356 2166 143 71 1545
Cuts at: 11012 1132
Size: 2229
Fragments arranged by size:
3757 2229 2166 1745 1545 356 143 97
71
PasI CC'CwG_GG
Cuts at: 1348 3013 5818 1348
Size: 1665 2805 7639
Fragments arranged by size:
7639 2805 1665
PciI A'CATG_T
Cuts at: 11008 11008
Size: 12109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3247 1108
Size: 2139 9970
PfoI T'CCnGG_A
Cuts at: 2800 3498 6807 6896 8401 9207 2800
Size: 698 3309 89 1505 806 5702
Fragments arranged by size:
5702 3309 1505 806 698 89
PmeI GTTT'AAAC
Cuts at: 9413 9413
Size: 12109
PmlI CAC'GTG
Cuts at: 3724 8939 3724
Size: 5215 6894
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10178 10210 11691 11723 10178
Size: 32 1481 32 10564
Fragments arranged by size:
10564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4485 4664 7644 8011 4485
Size: 179 2980 367 8583
Fragments arranged by size:
8583 2980 367 179
PshAI GACnn'nnGTC
Cuts at: 1613 6853 6990 7071 1613
Size: 5240 137 81 6651
Fragments arranged by size:
6651 5240 137 81
PsiI TTA'TAA
Cuts at: 9679 9679
Size: 12109
PspXI vC'TCGA_Gb
Cuts at: 2996 2996
Size: 12109
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 8095 8127 8095
Size: 32 12077
PstI C_TGCA'G
Cuts at: 830 4396 5164 5875 7423 8140 8209 9016 9461
Size: 3566 768 711 1548 717 69 807 445
Cuts at: 9461 830
Size: 3478
Fragments arranged by size:
3566 3478 1548 807 768 717 711 445
69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12109
PvuII CAG'CTG
Cuts at: 4262 4397 4424 4439 4576 5738 4262
Size: 135 27 15 137 1162 10633
Fragments arranged by size:
10633 1162 137 135 27 15
SacI G_AGCT'C
Cuts at: 2187 2454 2997 6686 7164 2187
Size: 267 543 3689 478 7132
Fragments arranged by size:
7132 3689 543 478 267
SalI G'TCGA_C
Cuts at: 1936 5150 6993 9451 1936
Size: 3214 1843 2458 4594
Fragments arranged by size:
4594 3214 2458 1843
SanDI GG'GwC_CC
Cuts at: 8011 8011
Size: 12109
SapI GCTCTTCn'nnn_
Cuts at: 3831 5762 3831
Size: 1931 10178
SbfI CC_TGCA'GG
Cuts at: 9461 9461
Size: 12109
ScaI AGT'ACT
Cuts at: 10357 12069 10357
Size: 1712 10397
SexAI A'CCwGG_T
Cuts at: 7597 7640 7597
Size: 43 12066
SfcI C'TryA_G
Cuts at: 826 1045 4392 5160 5871 6051 7014 7069 7419
Size: 219 3347 768 711 180 963 55 350
Cuts at: 7419 8136 8205 9012 9457 10592 11273 11464 826
Size: 717 69 807 445 1135 681 191 1471
Fragments arranged by size:
3347 1471 1135 963 807 768 717 711
681 445 350 219 191 180 69 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12109
SmaI CCC'GGG
Cuts at: 1872 9436 1872
Size: 7564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2996 3263 5421 8324 10213 11114
Size: 197 926 1053 267 2158 2903 1889 901
Cuts at: 11114 11376 820
Size: 262 1553
Fragments arranged by size:
2903 2158 1889 1553 1053 926 901 267
262 197
SnaBI TAC'GTA
Cuts at: 493 9900 493
Size: 9407 2702
SpeI A'CTAG_T
Cuts at: 152 6356 152
Size: 6204 5905
SphI G_CATG'C
Cuts at: 1132 6731 9396 9467 1132
Size: 5599 2665 71 3774
Fragments arranged by size:
5599 3774 2665 71
SspI AAT'ATT
Cuts at: 5 52 2638 10033 5
Size: 47 2586 7395 2081
Fragments arranged by size:
7395 2586 2081 47
StuI AGG'CCT
Cuts at: 1517 7187 8675 9063 1517
Size: 5670 1488 388 4563
Fragments arranged by size:
5670 4563 1488 388
StyI C'CwwG_G
Cuts at: 513 1065 2004 3903 4515 4938 5400 5640 6141
Size: 552 939 1899 612 423 462 240 501
Cuts at: 6141 8658 8676 8805 9548 513
Size: 2517 18 129 743 3074
Fragments arranged by size:
3074 2517 1899 939 743 612 552 501
462 423 240 129 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2162 2552 2925 2948 3500 3569 3751
Size: 1255 582 390 373 23 552 69 182
Cuts at: 3751 5013 5810 6865 7010 7154 7697 8492 10136
Size: 1262 797 1055 145 144 543 795 1644
Cuts at: 10136 10153 10306 10491 10936 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1644 1498 1262 1255 1055 797 795 582
552 543 445 390 373 185 182 153
145 144 69 23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3489 10355 12067
Size: 274 80 33 51 1443 1512 6866 1712
Cuts at: 12067 96
Size: 138
Fragments arranged by size:
6866 1712 1512 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2524 2556 4648 4680 7732 7764 8902 8934 12069
Size: 32 2092 32 3052 32 1138 32 3135
Cuts at: 12069 12101 2524
Size: 32 2532
Fragments arranged by size:
3135 3052 2532 2092 1138 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5461 8761 9040 1316
Size: 4145 3300 279 4385
Fragments arranged by size:
4385 4145 3300 279
XbaI T'CTAG_A
Cuts at: 3526 7943 9445 3526
Size: 4417 1502 6190
Fragments arranged by size:
6190 4417 1502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 5710 6670 7567 2011
Size: 3699 960 897 6553
Fragments arranged by size:
6553 3699 960 897
XhoI C'TCGA_G
Cuts at: 1943 2996 5421 1943
Size: 1053 2425 8631
Fragments arranged by size:
8631 2425 1053
XmaI C'CCGG_G
Cuts at: 1870 9434 1870
Size: 7564 4545
XmnI GAAnn'nnTTC
Cuts at: 2840 3357 6240 10238 2840
Size: 517 2883 3998 4711
Fragments arranged by size:
4711 3998 2883 517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9917 276
Size: 53 83 186 936 8383 2468
Fragments arranged by size:
8383 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI RsrII SacII SfiI SfoI SrfI SwaI